Hampton Dialysis Buttons 透析按钮
透析按钮
应用
- 透析结晶,蛋白质折叠,小容量样品透析
特征
- 低容量透析
- 适用于24个井板
- 一遍又一遍地使用
描述
在透析法中,样品被半透膜从“沉淀剂”中分离出来,这种膜允许小的试剂分子通过,但防止生物大分子穿过膜。1-3
透析按钮是注射成型的聚苯乙烯,大小与一个小按钮。样品被放置在这个房间,以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部,并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米,外径约为10毫米而没有O形环,以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米,外径约为15毫米,没有O形环,而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头,以协助将透析膜应用到按钮上,并与所提供的o-环固定在一起。
Dialysis Buttons
APPLICATIONS
- Crystallization by dialysis, protein folding, & small volume sample dialysis
FEATURES
- Low volume dialysis
- Fits in 24 well plates
- Use over and over again
DESCRIPTION
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3
Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.
CAT NO | NAME | DESCRIPTION |
HR3-336 | Dialysis Buttons Sampler | 5 of each size listed below |
HR3-314 | 5 µl Dialysis Button | 50 pack |
HR3-316 | 10 µl Dialysis Button | 50 pack |
HR3-318 | 15 µl Dialysis Button | 50 pack |
HR3-320 | 20 µl Dialysis Button | 50 pack |
HR3-326 | 50 µl Dialysis Button | 50 pack |
HR3-328 | 100 µl Dialysis Button | 50 pack |
HR3-330 | 200 µl Dialysis Button | 50 pack |
HR3-332 | 350 µl Dialysis Button | 50 pack |
RELATED ITEM(S)
REFERENCES
1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.