Hampton 产生蛋白质晶体的种子试剂盒 Seed Bead™ Kits

Hampton 产生蛋白质晶体的种子试剂盒 Seed Bead™ Kits

Hampton research蛋白结晶试剂代理–上海金畔生物
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Tools & Seeding > Seed Bead > Seed Bead™ Kits

应用

  • 产生蛋白质晶体的种子

特征

  • 容易产生一致的种子
  • 用连续稀释法控制种子进入滴滴的数量。
  • 微种基质筛选(MMS)种子库
  • 聚四氟乙烯、不锈钢、硅酸锆陶瓷或玻璃种子珠

描述

种子珠套件用于创建种子库,以执行后续的种子试验。种子珠袋包含24个种子珠管,这是一种特殊的1.5毫升微离心管,形状可以在混合过程中将种子保持在样品中。每个管配置聚四氟乙烯,不锈钢,锆硅酸盐陶瓷,或玻璃种子珠。

种子允许在亚稳态区域生长晶体。在这个区域的结晶提供了控制,重现性和提高成功结晶实验的可能性。另外,晶体可以从种子中生长,但不能自发成核。通过将种子或溶液放置在一滴饱和到亚稳态区域的种子中,人们可以利用种子生长更大的单晶。通过控制引入液滴的种子数量,就可以控制生长的晶体数量。实际上不可能测量和知道引入一滴种子的数量,但通过对浓缩种子进行连续稀释,就可以控制滴滴中生长的晶体数量。

利用种子珠套件,我们可以为后续的播种实验创建水晶种子库。晶体被放置在带有种子珠的微离心管中,可以是涡状的,也可以是声纳的,以产生种子。然后,通过进行系列稀释,可以控制实验中晶体的数量和尺寸。

种子珠工具包有助于为自动化和半自动微型播种准备种子储存(D‘Arcy,2007年;Harlos,2008年)。

种子珠袋(HR2-320)包含24个特殊的微离心管,每管包含一个单一的3mm聚四氟乙烯种子珠。

种子珠钢盒(HR4-780)包含24根特殊的微离心管,每管含有6颗1.6毫米不锈钢种子珠。

种子珠陶瓷盒(HR4-781)包含24个特殊的微离心管,每管含有10颗1.0毫米硅酸锆陶瓷种子珠。

种子珠玻璃盒(HR4-782)包含24个特殊的微离心管,每管含有10颗1.0毫米的玻璃种子珠。

APPLICATIONS

  • Generate seeds of protein crystals

FEATURES

  • Easily generate consistent seed stocks
  • Use serial dilution to control the number of seeds introduced into the drop
  • Microseed matrix screening (MMS) seed stock
  • PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Bead

DESCRIPTION

The Seed Bead kits are used to create a seed stock for performing subsequent seeding experiments. The Seed Bead kits contains 24 Seed Bead Tubes, a special 1.5 ml microcentrifuge tube, shaped to maintain the seed in the sample during mixing. Each tube is configured with a PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Beads.

Seeding allows one to grow crystals in the metastable zone. Crystallization in this zone provides control, reproducibility and an improved likelihood of a successful crystallization experiment. Also, crystals can grow from seeds but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated to the metastable zone, one can use the seeds to grow larger single crystals. By controlling the number of seeds introduced into the drop, one can control the number of crystals grown. It is not practically possible to measure and know the number of seeds introduced to a drop, but by performing serial dilutions from a concentrated seed stock, one can control the number of crystals grown in the drop.

Using the Seed Bead kits, one can create crystal seed stock for subsequent seeding experiments. Crystals are placed in the microcentrifuge tube with the Seed Bead(s) and either vortexed or sonicated to generate a seed stock. Then by performing serial dilutions, one can control the number and size of crystals in the experiment.

The Seed Bead kit is useful for the preparation of seed stocks for automated and semi-automated microseeding (D’Arcy 2007, Harlos 2008).

Each Seed Bead Kit (HR2-320) contains 24 special microcentrifuge tubes, each tube containing a single 3mm PTFE Seed Bead.

Each Seed Bead Steel Kit (HR4-780) contains 24 special microcentrifuge tubes, each tube containing a six 1.6 mm Stainless Steel Seed Beads.

Each Seed Bead Ceramic Kit (HR4-781) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Zirconium Silicate Ceramic Seed Beads.

Each Seed Bead Glass Kit (HR4-782)

PTFE - Steel - Ceramic - Glass

HR2-320 Seed Bead Kit 24 tubes with PTFE Seed Bead
HR4-780 Seed Bead Steel Kit 24 tubes with Steel Seed Beads
HR4-781 Seed Bead Ceramic Kit 24 tubes with Ceramic Seed Beads
HR4-782 Seed Bead Glass Kit 24 tubes with Glass Seed Beads