Hampton 蛋白结晶96孔板 Diaplate
Hampton蛋白结晶试剂耗材
Hampton Research代理
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APPLICATIONS
- Protein and peptide purification/concentration
- Variation of crystallisation conditions for crystal growth
- Removal of dyes after protein labelling
- Electro-elution of proteins
- Protein and DNA desalting/buffer exchange
- Protein in vitro translation
- Glycoprotein modification and engineering
- Enzyme activity/binding assays/detergent removal
FEATURES
- Protein crystallisation applications allow for unique matrix changing
- Simple handling, ready to use, easy sample recovery
- Complete kit, no additional materials required
- Complies with ANSI/SBS standards for automation
- Pipetting can be performed manually or using a robot
- Ideal for the desalting of up to 3.2µl protein solution
- No cross contamination or leakage between wells guaranteed
- 96 individual regenerated cellulose membranes; sulphur and heavy metal free
- Molecular weight cut off of 10,000 Daltons
- Low protein binding and high protein retention
- Gentle and efficient desalting without disturbing valuable protein complexes
- High throughput; simultaneous processing of 96 experiments
- Reliable and uniform, stable construction
- Single use – disposable device; no cleaning required
DESCRIPTION
The disposable Diaplate is a 96 well micro-dialysis plate ideal for the desalting of protein from very small volumes of up to a maximum of 3.2 µl.
The sample solution is pipetted into each of the 96 wells with up to 0.35ml dialysis solution used for exchange. The Diaplate is ANSI/SLAS 1 2004 standards providing high throughput use with robotic dispensing. Dialysis is recommended for 2-3 hours and then the dialysis buffer is changed being left for another 2-3 hours to achieve over 90% exchange. Further dialysis steps can be carried out as required. Incubation, rocking table for speed and moisture chamber for full humidification can be applied to the experiment if desired.
Each of the 96 wells has a separate regenerated cellulose membrane inside meaning no cross contamination or leakage between wells. The size of the compounds that pass through the membrane are determined by its Molecular Weight Cut Off (MWCO). The Swissci Diaplate comes as standard with a specially selected membrane that has a MWCOs of 10,000 Daltons. The Diaplate kit includes: Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron UVP Cover Film, engraved UV Protective Cover and a sealing Paddle. Swissci W82010.
Diaplate Specifications
Membrane molecular weight cut-off (MWCO) 10kDa (10,000 Daltons)
Maximum sample capacity 3.2 µl
Maximum dialysate or reagent capacity 350 µl
Plate dimensions 127.76 +/- 0.5 x 85.48 +/- 0.5 mm
应用
蛋白质和肽纯化/浓缩
晶体生长的结晶条件的变化
蛋白质标记后染料的去除
蛋白质的电洗脱
蛋白质和 DNA 脱盐/缓冲液交换
蛋白质体外翻译
糖蛋白修饰和工程
酶活性/结合测定/去垢剂去除
特征
蛋白质结晶应用允许独特的基质变化
操作简单，随时可用，样品回收容易
完整套件，无需额外材料
符合 ANSI/SBS 自动化标准
移液可以手动或使用机器人进行
最适合对高达 3.2µl 的蛋白质溶液进行脱盐
保证井间无交叉污染或泄漏
96 个单独的再生纤维素膜；不含硫和重金属
截留分子量为 10,000 道尔顿
低蛋白质结合和高蛋白质保留
温和高效的脱盐，不会干扰有价值的蛋白质复合物
高吞吐量；同时处理 96 个实验
可靠均匀，结构稳定
一次性使用 – 一次性设备；无需清洁
描述
一次性 Diaplate 是一种 96 孔微量透析板，非常适合从极小体积到最大 3.2 µl 的蛋白质脱盐。
将样品溶液移入 96 个孔中的每个孔中，最多 0.35ml 透析溶液用于交换。 Diaplate 符合 ANSI/SLAS 1 2004 标准，可通过机器人点胶实现高吞吐量使用。建议透析 2-3 小时，然后更换透析缓冲液，再放置 2-3 小时以达到 90% 以上的交换。可以根据需要进行进一步的透析步骤。如果需要，可以将孵化、速度摇床和湿度室用于完全加湿。
96 个孔中的每一个都有一个单独的再生纤维素膜，这意味着孔之间没有交叉污染或泄漏。通过膜的化合物的大小由其截留分子量 (MWCO) 决定。 Swissci Diaplate 标配特别选择的膜，其 MWCO 为 10,000 道尔顿。 Diaplate 套件包括： Diaplate 与 200 微米压力粘合剂垫片、200 微米 UVP 覆盖膜、雕刻的 UV 保护盖和密封桨。 Swissci W82010。
表盘规格
膜截留分子量 (MWCO) 10kDa（10,000 道尔顿）
最大样品容量 3.2 µl
最大透析液或试剂容量 350 µl
板尺寸 127.76 +/- 0.5 x 85.48 +/- 0.5 毫米
| HR3-253 | Diaplate | 10 kDa MWCO 3/pack |