Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications

Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品

Features

  Membrane protein sparse matrix screens

  Sparse matrix formula efficiently samples salts, polymers, organics and pH

  pH range 4.6 – 8.5

  Formulated for use with detergents

   Tube or Deep Well block format

   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format

Hampton 聚乙二醇4,000 Polyethylene glycol 4,000

Hampton 聚乙二醇4,000 Polyethylene glycol 4,000

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势:货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimize Reagents > Optimize – Polymers > Polyethylene glycol 4,000

聚乙二醇4,000

应用

  • 结晶级聚乙二醇4,000用于筛选或优化

特征

  • 无菌过滤液
  • 在1+超纯水中配制:在25°C下电阻率为18.2兆,总有机碳<5 ppb,无细菌(<1细菌(CFU/ml)),无热原(<0.03内毒素(EU/ml)),无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

描述

聚乙二醇4,000

同义词:PEG 4,000
H(Och)22)n
Mr 3,500-4,500
核证机关编号[25322-68-3]
教统会编号500-038-2
默克14,7568
RTECS TQ 3500000
MDL号码MFCD 00081839
PubChem物质ID 24890217
熔点(原料)58.0-61.0℃
醛无检出
过氧化物无检出

HR2-529测量电导率范围:34.0-46.9微秒/厘米,25°C
HR2-529测量折射率范围:1.40172~1.40279℃,20°C

Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

CAT NO NAME DESCRIPTION
HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton Dialysis Buttons 透析按钮

Hampton Dialysis Buttons 透析按钮

透析按钮

应用

  • 透析结晶,蛋白质折叠,小容量样品透析

特征

  • 低容量透析
  • 适用于24个井板
  • 一遍又一遍地使用

描述

在透析法中,样品被半透膜从“沉淀剂”中分离出来,这种膜允许小的试剂分子通过,但防止生物大分子穿过膜。1-3

透析按钮是注射成型的聚苯乙烯,大小与一个小按钮。样品被放置在这个房间,以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部,并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米,外径约为10毫米而没有O形环,以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米,外径约为15毫米,没有O形环,而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头,以协助将透析膜应用到按钮上,并与所提供的o-环固定在一起。

Dialysis Buttons

APPLICATIONS

  • Crystallization by dialysis, protein folding, & small volume sample dialysis

FEATURES

  • Low volume dialysis
  • Fits in 24 well plates
  • Use over and over again

DESCRIPTION

In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3

Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

CAT NO NAME DESCRIPTION
HR3-336 Dialysis Buttons Sampler 5 of each size listed below
HR3-314 5 µl Dialysis Button 50 pack
HR3-316 10 µl Dialysis Button 50 pack
HR3-318 15 µl Dialysis Button 50 pack
HR3-320 20 µl Dialysis Button 50 pack
HR3-326 50 µl Dialysis Button 50 pack
HR3-328 100 µl Dialysis Button 50 pack
HR3-330 200 µl Dialysis Button 50 pack
HR3-332 350 µl Dialysis Button 50 pack

RELATED ITEM(S)

REFERENCES

1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).

2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).

3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.

4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

Hampton 蛋白结晶删选试剂– Index HT

Hampton 蛋白结晶删选试剂– Index HT

Applications

Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

多样结晶试剂系统删选试剂盒,用于蛋白质,复合物,肽,核酸,和水溶性的小分子。

Features

 Developed at Hampton Research

Screens classic, contemporary, & modern crystallization reagents

Samples pH 3 to 9

Compatible with microbatch, vapor diffusion, & liquid diffusion methods

Specially formulated reagent zones:

1 .Traditional salts versus pH

2.Neutralized organic acids

3.High [salt] with low [polymer]

4.High [polymer] with low [salt]

5.Low ionic strength versus pH

6.PEG & Salt versus pH

7.PEG & Salt

 Tube or Deep Well block format

Description
Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format.

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.

Index contains 96 unique reagents, 10 ml each.

Index HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C

Apo Kinase crystal. Initial hit from the Hampton Research Index Screen. Annie Hassell, Glaxo Smithkline.

Index • Index HT

Crystal of a heme-based sensor from Thermoanaerobacter Tengcongensis grown using the Hampton Research Index screen. Patricia Pellicena & John Kuriyan at University of California, Berkeley, USA.

CAT NO NAME DESCRIPTION
HR2-144 Index 10 ml, tube format
HR2-134 Index HT 1 ml, Deep Well block format

Hampton HEPES sodium Buffer

Hampton HEPES sodium Buffer

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS

  • Crystallization grade HEPES sodium buffer for formulating screens or for optimization

FEATURES

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

HEPES sodium

Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C

HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

HR2-577 1.0 M HEPES sodium 100 mL
HR2-733 1.0 M HEPES sodium pH 7.5 100 mL

RELATED ITEM(S)  相关产品

REFERENCES 参考文献

1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.