Hampton 蛋白结晶试剂–Crystal Screen
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
应用
- 蛋白质、可溶性肽、核酸和水溶性小分子的初级筛选
- 稀疏矩阵加性筛
特征
- 原始稀疏矩阵屏幕
- 稀疏矩阵公式有效取样盐、聚合物、有机物和pH
- 对1,000多个生物大分子有效
- 管式或深井块格式
描述
设计了晶体屏和晶体屏2试剂试剂盒，为大分子结晶提供了一种高效、快速的筛选方法。该筛具有简单实用的特点，可用于寻找初始结晶条件。对1000多个蛋白质、多肽、寡核苷酸和小分子的初始结晶条件进行了晶体筛选。
克服穷尽寻找合适结晶条件的一种非常有效的方法是使用稀疏矩阵试制条件法，这种方法是偏倚的，并从已知的大分子结晶条件中选择。在晶体屏幕和晶体屏幕2中使用的配方评估了96种独特的pH、盐类、聚合物和有机物的混合物，以及它们促进晶体生长的能力。
水晶屏幕包含50种独特的试剂，每个10毫升，是基于稀疏矩阵公式首次描述由Jancarik和Kim在1991年。
水晶屏幕2，一个水晶屏幕的延伸，包含48种独特的试剂，每个10毫升，是基于1994年由Cudney等人首次描述的配方。
晶体屏幕HT包含1ml的每个试剂1-48从水晶屏幕和所有48试剂从水晶屏幕2在一个单一的深井块格式。
准备使用的试剂是无菌过滤和配制超纯1型水，使用最高纯度的盐类，聚合物，有机物和缓冲液.个别试剂可通过汉普顿研究定制商店获得。
Applications
Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
Sparse matrix additive screen
Features
The original sparse matrix screen
Sparse matrix formula efficiently samples salts, polymers, organics, & pH
Proven effective with more than 1,000 biological macromolecules
Tube or Deep Well block format
Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.
A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.
Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.
Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.
Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.
Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.
| CAT NO | NAME | DESCRIPTION |
| HR2-110 | Crystal Screen | 10 ml, tube format |
| HR2-112 | Crystal Screen 2 | 10 ml, tube format |
| HR2-130 | Crystal Screen HT | 1 ml, Deep Well block format |