Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents单独成分

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents单独成分

APPLICATIONS

  • Individual reagents from SaltRx 1, SaltRx 2, and SaltRx HT for screening, optimization and production

FEATURES

  • 185 ml volume
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

HR2-907-** and HR2-909-**
** Refers to the reagent number in the kit.
For example, SaltRx 1 reagent number 1 = SaltRx HT reagent A1 = HR2-907-01
For example, SaltRx 2 reagent number 1 = SaltRx HT reagent E1 = HR2-909-01

CAT NO

HR2-907-01

NAME

SaltRx 1 #01 / SaltRx HT A1 185ml

DESCRIPTION

1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-02

NAME

SaltRx 1 #02 / SaltRx HT A2 185ml

DESCRIPTION

2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-03

NAME

SaltRx 1 #03 / SaltRx HT A3 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-04

NAME

SaltRx 1 #04 / SaltRx HT A4 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-05

NAME

SaltRx 1 #05 / SaltRx HT A5 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-06

NAME

SaltRx 1 #06 / SaltRx HT A6 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-07

NAME

SaltRx 1 #07 / SaltRx HT A7 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-08

NAME

SaltRx 1 #08 / SaltRx HT A8 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-09

NAME

SaltRx 1 #09 / SaltRx HT A9 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-10

NAME

SaltRx 1 #10 / SaltRx HT A10 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-11

NAME

SaltRx 1 #11 / SaltRx HT A11 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-12

NAME

SaltRx 1 #12 / SaltRx HT A12 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-13

NAME

SaltRx 1 #13 / SaltRx HT B1 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-14

NAME

SaltRx 1 #14 / SaltRx HT B2 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-15

NAME

SaltRx 1 #15 / SaltRx HT B3 185ml

DESCRIPTION

1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-16

NAME

SaltRx 1 #16 / SaltRx HT B4 185ml

DESCRIPTION

1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-17

NAME

SaltRx 1 #17 / SaltRx HT B5 185ml

DESCRIPTION

1.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-18

NAME

SaltRx 1 #18 / SaltRx HT B6 185ml

DESCRIPTION

2.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-19

NAME

SaltRx 1 #19 / SaltRx HT B7 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-20

NAME

SaltRx 1 #20 / SaltRx HT B8 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-21

NAME

SaltRx 1 #21 / SaltRx HT B9 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-22

NAME

SaltRx 1 #22 / SaltRx HT B10 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-23

NAME

SaltRx 1 #23 / SaltRx HT B11 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-24

NAME

SaltRx 1 #24 / SaltRx HT B12 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-25

NAME

SaltRx 1 #25 / SaltRx HT C1 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-26

NAME

SaltRx 1 #26 / SaltRx HT C2 185ml

DESCRIPTION

0.7 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-27

NAME

SaltRx 1 #27 / SaltRx HT C3 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-28

NAME

SaltRx 1 #28 / SaltRx HT C4 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-29

NAME

SaltRx 1 #29 / SaltRx HT C5 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-30

NAME

SaltRx 1 #30 / SaltRx HT C6 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-31

NAME

SaltRx 1 #31 / SaltRx HT C7 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-32

NAME

SaltRx 1 #32 / SaltRx HT C8 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-33

NAME

SaltRx 1 #33 / SaltRx HT C9 185ml

DESCRIPTION

1.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-34

NAME

SaltRx 1 #34 / SaltRx HT C10 185ml

DESCRIPTION

2.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-35

NAME

SaltRx 1 #35 / SaltRx HT C11 185ml

DESCRIPTION

1.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-36

NAME

SaltRx 1 #36 / SaltRx HT C12 185ml

DESCRIPTION

2.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-37

NAME

SaltRx 1 #37 / SaltRx HT D1 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-38

NAME

SaltRx 1 #38 / SaltRx HT D2 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-39

NAME

SaltRx 1 #39 / SaltRx HT D3 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-40

NAME

SaltRx 1 #40 / SaltRx HT D4 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-41

NAME

SaltRx 1 #41 / SaltRx HT D5 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-42

NAME

SaltRx 1 #42 / SaltRx HT D6 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-43

NAME

SaltRx 1 #43 / SaltRx HT D7 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-44

NAME

SaltRx 1 #44 / SaltRx HT D8 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-45

NAME

SaltRx 1 #45 / SaltRx HT D9 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-46

NAME

SaltRx 1 #46 / SaltRx HT D10 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-47

NAME

SaltRx 1 #47 / SaltRx HT D11 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-48

NAME

SaltRx 1 #48 / SaltRx HT D12 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-01

NAME

SaltRx 2 #01 / SaltRx HT E1 185ml

DESCRIPTION

1.0 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-02

NAME

SaltRx 2 #02 / SaltRx HT E2 185ml

DESCRIPTION

1.8 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-03

NAME

SaltRx 2 #03 / SaltRx HT E3 185ml

DESCRIPTION

1.5 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-04

NAME

SaltRx 2 #04 / SaltRx HT E4 185ml

DESCRIPTION

2.4 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-05

NAME

SaltRx 2 #05 / SaltRx HT E5 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 (0.98 M Sodium phosphate monobasic monohydrate, 0.02 M Potassium phosphate dibasic)

 CAT NO

HR2-909-06

NAME

SaltRx 2 #06 / SaltRx HT E6 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 (0.35 M Sodium phosphate monobasic monohydrate, 0.65 M Potassium phosphate dibasic)

 CAT NO

HR2-909-07

NAME

SaltRx 2 #07 / SaltRx HT E7 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 (0.04 M Sodium phosphate monobasic monohydrate, 0.96 M Potassium phosphate dibasic)

 CAT NO

HR2-909-08

NAME

SaltRx 2 #08 / SaltRx HT E8 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 (1.764 M Sodium phosphate monobasic monohydrate, 0.036 M Potassium phosphate dibasic)

 CAT NO

HR2-909-09

NAME

SaltRx 2 #09 / SaltRx HT E9 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 (0.63 M Sodium phosphate monobasic monohydrate, 1.17 M Potassium phosphate dibasic)

 CAT NO

HR2-909-10

NAME

SaltRx 2 #10 / SaltRx HT E10 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 (0.072 M Sodium phosphate monobasic monohydrate, 1.728 M Potassium phosphate dibasic)

 CAT NO

HR2-909-11

NAME

SaltRx 2 #11 / SaltRx HT E11 185ml

DESCRIPTION

0.5 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-12

NAME

SaltRx 2 #12 / SaltRx HT E12 185ml

DESCRIPTION

1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-13

NAME

SaltRx 2 #13 / SaltRx HT F1 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-14

NAME

SaltRx 2 #14 / SaltRx HT F2 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-15

NAME

SaltRx 2 #15 / SaltRx HT F3 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-16

NAME

SaltRx 2 #16 / SaltRx HT F4 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-17

NAME

SaltRx 2 #17 / SaltRx HT F5 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-18

NAME

SaltRx 2 #18 / SaltRx HT F6 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-19

NAME

SaltRx 2 #19 / SaltRx HT F7 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-20

NAME

SaltRx 2 #20 / SaltRx HT F8 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-21

NAME

SaltRx 2 #21 / SaltRx HT F9 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-22

NAME

SaltRx 2 #22 / SaltRx HT F10 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-23

NAME

SaltRx 2 #23 / SaltRx HT F11 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-24

NAME

SaltRx 2 #24 / SaltRx HT F12 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-25

NAME

SaltRx 2 #25 / SaltRx HT G1 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-26

NAME

SaltRx 2 #26 / SaltRx HT G2 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-27

NAME

SaltRx 2 #27 / SaltRx HT G3 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-28

NAME

SaltRx 2 #28 / SaltRx HT G4 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-29

NAME

SaltRx 2 #29 / SaltRx HT G5 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-30

NAME

SaltRx 2 #30 / SaltRx HT G6 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-31

NAME

SaltRx 2 #31 / SaltRx HT G7 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-32

NAME

SaltRx 2 #32 / SaltRx HT G8 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-33

NAME

SaltRx 2 #33 / SaltRx HT G9 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-34

NAME

SaltRx 2 #34 / SaltRx HT G10 185ml

DESCRIPTION

1.0 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-35

NAME

SaltRx 2 #35 / SaltRx HT G11 185ml

DESCRIPTION

1.3 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-36

NAME

SaltRx 2 #36 / SaltRx HT G12 185ml

DESCRIPTION

1.4 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-37

NAME

SaltRx 2 #37 / SaltRx HT H1 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-38

NAME

SaltRx 2 #38 / SaltRx HT H2 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-39

NAME

SaltRx 2 #39 / SaltRx HT H3 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-40

NAME

SaltRx 2 #40 / SaltRx HT H4 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-41

NAME

SaltRx 2 #41 / SaltRx HT H5 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-42

NAME

SaltRx 2 #42 / SaltRx HT H6 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-43

NAME

SaltRx 2 #43 / SaltRx HT H7 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-44

NAME

SaltRx 2 #44 / SaltRx HT H8 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-45

NAME

SaltRx 2 #45 / SaltRx HT H9 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-46

NAME

SaltRx 2 #46 / SaltRx HT H10 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-47

NAME

SaltRx 2 #47 / SaltRx HT H11 185ml

DESCRIPTION

35% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-48

NAME

SaltRx 2 #48 / SaltRx HT H12 185ml

DESCRIPTION

60% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

Mitegen Crystal Mounts and Loops环

Mitegen Crystal Mounts and Loops环

Crystal Mounts and Loops

Simplify crystal harvesting, and obtain the best possible data from your crystals, using the most advanced lithographic cryo-loops available.

Composed of X-ray transparent material, crystals can be mounted directly on the loops, reducing excess fluid, preventing damage, and making getting the crystals out of the drop easier.

简化晶体收获,并获得最好的数据,从你的晶体,使用最先进的光刻低温循环可用。

晶体由x射线透明材料组成,可以直接安装在回路上,减少多余的液体,防止损坏,并使晶体更容易从液滴中取出。

MicroMounts™ are the world’s highest performance tools for retrieving and mounting protein crystals, virus crystals, and small molecule/inorganic crystals. They can be also used for handling small fragile samples of all sorts, including biological, archaeological and mineralogical samples. MicroMounts™ are compatible with all standard X-ray hardware, and can be inserted in 0.7 mm mechanical pencils or micromanipulators for easy handling.Micromounts™ consist of thin polymer tips attached to beveled non-magnetic 0.025″ (0.64 mm) diameter stainless steel rods. Their patented design provides an excellent combination of X-ray and optical transparency, mechanical rigidity and flexibility, and precision dimensions.

The polymer is unoriented, so the background scatter does not show the sharp peaks of competing mounts, and has the lowest possible birefringence.

Curving the tip by wrapping its base around the rod makes it rigid, and provides a scoop-like action in retrieving samples. The tip can also flex and bend flat against e.g., a well or glass slide, to dislodge and then slip under a sample.

A wicking aperture draws excess liquid away from the sample, reducing background scatter and allowing faster sample cooling for cryocrystallography.

Since 2008, MicroMounts™ (and all of our other products) have been made using the most mechanically robust unoriented polymer available. With proper care – especially during cleaning – each MicroMount™ can be used to harvest and measure dozens of crystals.

Extremely easy to use, novices can be mounting samples in minutes. MicroMounts™ make your crystallography pipeline more efficient by maximizing the odds of successful mounting, cooling and data collection.

MicroMounts™ 是世界上用于回收和安装蛋白质晶体、病毒晶体和小分子/无机晶体的最高性能工具。它们还可用于处理各种小型易碎样品,包括生物、考古和矿物样品。MicroMounts™ 与所有标准 X 射线硬件兼容,可插入 0.7 毫米自动铅笔或显微操作器中,以便于操作。

Micromounts™ 由薄聚合物尖端组成,连接到斜面非磁性 0.025 英寸(0.64 毫米)直径不锈钢杆。其专利设计完美结合了 X 射线和光学透明度、机械刚性和灵活性以及精密尺寸。

该聚合物是无取向的,因此背景散射不会显示竞争支架的尖峰,并且具有尽可能低的双折射。

通过将其基部缠绕在杆上来弯曲尖端,使其变得坚硬,并在取回样品时提供类似勺子的动作。尖端还可以相对于例如孔或载玻片平直地弯曲和弯曲,以移动并然后滑到样品下方。

芯吸孔将多余的液体从样品中吸走,减少背景散射,并允许更快地冷却样品以进行冷冻晶体学。

自 2008 年以来,MicroMounts™(以及我们所有其他产品)均采用现有机械性能最坚固的无取向聚合物制成。通过适当的保养——尤其是在清洁过程中——每个 MicroMount™ 都可以用来收获和测量数十个晶体。

使用极其简单,新手可以在几分钟内安装样品。MicroMounts™ 通过最大限度地提高成功安装、冷却和数据收集的几率,使您的晶体学流程更加高效。

MicroMounts™ consist of a thin microfabricated polyimide film attached to a solid non-magnetic 0.025″ (0.64 mm) stainless steel rod.

Films

Polyimide:
Used in Kapton® tape, which is employed for X-ray transparent windows on X-ray beam lines. Has low atomic number (Z) constituents and low density, and produces less background scatter than e.g., nylon. Optically transparent with an orange-gold hue.

Film Curvature:
Obtained by wrapping polyimide film around the steel rod. Provides excellent stiffness even with very thin (7.5 micrometer) films, and a convenient, scoop-like action in retrieving and handling samples.

Film Pattern

Sample aperture (A) sizes from 10 to 600 micrometers, with minimal polyimide width (B) around the aperture to minimize background scatter in all orientations.

Wicking hole (C) connected via drainage channel (D) to the sample aperture. Hole size is compatible with size 15 or XF paper wicks. “Fountain-pen”-like design allows easier removal of excess liquid – without ever touching the sample with the wick.

Alignment cross (E) located a fixed distance from the center of the sample aperture. Allows easy automated alignment of the sample aperture (A).

Sample aperture size code (F) around the alignment cross. Allows automated recognition of mount design and of aperture and sample size, which determines boundaries of aperture (to be avoided), required alignment tolerance, and area of sample available to be scanned for optimum diffraction. Initial beam size can then be automatically selected. Aperture size (G) in μm can be read directly off the mount.Orientation mark (H) allows the front and back of the mount to be automatically distinguished.

Rods
Solid 316 non-magnetic stainless steel rods. Diameter of 0.025″ (0.64 mm) is compatible with all standard goniometer bases / caps, and can be inserted and held in 0.7 mm mechanical pencils. Rods will not be pulled out or trapped by magnets in tools or in automounting hardware. Available in four standard lengths: 11 mm, 19 mm, SPINE, and 25 mm. The 11, 19 and 25 mm rod lengths give rod base-to-sample center distances comparable to those of nylon loops mounted in 10, 18, and 24 mm rods, respectively. Custom rod lengths available on request.

Unlike competing mounts in hollow rods, our solid rods will not suck up samples and liquid.

Beveled at film end. Allows good visibility of the sample for a wider range of rod orientations during sample retrieval than unbeveled rods.

Can be cut using spring-steel-compatible pliers/cutters to any desired length. Can be easily bent to place sample in desired orientation. Fit all standard bases.

MicroMounts™ 由附着在实心非磁性 0.025 英寸(0.64 毫米)不锈钢棒上的微加工聚酰亚胺薄膜组成。

聚酰亚胺:
用于 Kapton® 胶带,用于 X 射线光束线上的 X 射线透明窗口。具有低原子序数 (Z) 成分和低密度,并且比尼龙等产生更少的背景散射。光学透明,呈橙金色色调。

薄膜曲率:
通过将聚酰亚胺薄膜缠绕在钢棒上而获得。即使对于非常薄(7.5 微米)的薄膜也能提供出色的刚度,并在检索和处理样品时提供方便的勺状动作。

薄膜图案

样品孔径 (A) 尺寸为 10 至 600 微米,孔径周围具有最小的聚酰亚胺宽度 (B),以最大限度地减少所有方向的背景散射。

芯吸孔 (C) 通过排水通道 (D) 连接至样品孔。孔尺寸与 15 号或 XF 号纸芯兼容。“钢笔”式的设计可以更轻松地去除多余的液体,而无需用吸芯接触样品。

对准十字 (E) 位于距样品孔径中心固定距离的位置。可以轻松自动对准样品孔径 (A)。

对准十字周围的孔径尺寸代码 (F) 示例。允许自动识别安装设计以及孔径和样品尺寸,从而确定孔径边界(要避免)、所需的对准公差以及可用于扫描以获得最佳衍射的样品区域。然后可以自动选择初始光束尺寸。 孔径尺寸 (G)(以微米为单位)可以直接从安装座上读取。方向标记 (H) 可以自动区分安装座的正面和背面。

实心 316 无磁不锈钢棒。直径为 0.025 英寸(0.64 毫米),与所有标准测角仪底座/盖子兼容,并且可以插入并固定在 0.7 毫米自动铅笔中。杆不会被工具或自动安装硬件中的磁铁拉出或卡住。有四种标准长度可供选择:11 毫米、19 毫米、SPINE 和 25 毫米。11、19 和 25 毫米的杆长度使杆底座到样品的中心距离分别与安装在 10、18 和 24 毫米杆中的尼龙环的距离相当。可根据要求提供定制杆长度。

与空心棒中的竞争安装座不同,我们的实心棒不会吸收样品和液体。

薄膜末端斜切。与无斜角的杆相比,在样品检索过程中,可以在更广泛的杆方向范围内实现良好的样品可视性。

可以使用弹簧钢兼容的钳子/切割器切割成任何所需的长度。可以 轻松弯曲 以将样品放置在所需的方向。适合所有标准底座。

清洁 MiTeGen 工具的最佳且最简单的方法是将其浸泡在含有清洁剂的溶液中。当安装在 MiTeGen 底座支架上时,可以一次清洁大量安装件。

为了去除蛋白质残留物,我们推荐使用含酶洗涤剂,例如 Alconox 的 Tergazyme 或 Decon 的 Contrex EZ;否则,标准实验室玻璃器皿/塑料器皿清洁剂(例如 Alconox)就足够了。我们建议最初浸泡 10 到 15 分钟,然后在水浴中快速冲洗以去除洗涤剂。所需浸泡的最佳长度将取决于安装件或工具上残留的残留材料(蛋白质等)以及自上次使用以来的时间。使用后越早清洁它们就越容易。

MiTeGen 的底座支架 使清洁变得容易:

•将包含环的底座装入支架中。
•然后将支架放入塑料或玻璃桶或盘中。
•按照洗涤剂盒上的说明混合含有洗涤剂的溶液。
•然后将清洁剂倒入桶中,直至液位升至底座支架上的填充线。这将确保环形尖端被浸泡,同时保持底座的不锈钢部分远离溶液并防止腐蚀。
在极少数情况下,当浸泡无法去除沉积物时,您可以使用中号 (M) 或细号 (F) 纸芯。为了最方便地操作,请使用剃须刀刀片从最宽端切掉,使其直径适合 0.7 毫米自动铅笔。然后将另一端浸入水、清洁剂溶液或异丙醇中,并从底部到尖端轻轻抚摸聚合物。

处理注意事项:

MiTeGen 的 MicroMounts™、MicroLoops™ 和其他工具的微加工尖端由最耐用的无取向聚合物制成,可实现超低背景 X 射线散射。与尼龙等定向纤维相比,如果使用不当,非定向聚合物更容易撕裂或切割。请按照以下说明操作,以最大限度地延长我们产品的使用寿命。

1. 切勿 用手触摸尖端。始终沿销轴而不是尖端使用我们的重型镊子。

2. 切勿 将斜面钢销推入尖端抵靠坚硬的表面。


3. 应做: 将吸头固定在 MiTeGen 底座支架中,并浸泡在清洁剂中来清洁吸头。

请勿: 长时间超声处理。超声处理几分钟左右就可以了,但长时间超声处理可能会损坏一些尖端。使用含酶洗涤剂,无需超声处理。


4. 应做: 使用 MiTeGen 纸芯去除顽固残留物

请勿: 使用棉签或泡沫头棉签。棉签可以捕获并撕裂聚合物。


5. 清洁后要干燥吸头,请让其风干。您可以短暂浸入异丙醇中以加快干燥速度。 避免吹干。如果喷嘴距离尖端太近,产生的湍流阻力可能会损坏尖端。喷嘴距尖端至少 20 厘米。

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Mitegen Micromounts™ 和 MicroLoops环用于高分子晶体学使用指南

Mitegen Micromounts™ 和 MicroLoops环用于高分子晶体学使用指南

Using Micromounts™ and MicroLoops™ for Macromolecular Crystallography

MicroMounts™ and MicroLoops™ are used in much the same way as nylon loop mounts, and are much easier to use than glass fibers and other mounting methods traditionally used in small-molecule crystallography. Their non-magnetic stainless steel rods are compatible with all existing goniometer bases (caps) and mounting hardware. Our suggested procedures follow.

Select a mount with a sample aperture size slightly smaller than the crystal size. Because competing nylon loops and mylar mounts produce so much more background diffraction than MiTeGen’s products, most crystallographers try to mount their crystal in the middle of the aperture, far from the nylon or mylar, and held in place by a liquid meniscus spanning the loop. This keeps the X-ray beam away from the strongly scattering nylon or mylar. But all that extra liquid boosts your background and can crush your crystal when it freezes. With MiTeGen’s products, you never have to worry about background from the X-ray beam hitting the mount.

Mount your crystal so that it rests on top of the aperture, so as to minimize excess liquid and associated background X-ray scatter. Choosing a slightly smaller aperture also makes it much easier to fish the crystal out of its solution. You don’t “loop” your crystal, you scoop it! (Of course, you can still mount your crystals the old way if you’re more comfortable with that, but our approach will usually give you better data.)

Insert the stainless steel rod of the MicroMount™ / MicroLoop™ into one of MiTeGen’s reusable goniometer bases, or else into a standard goniometer base using a small amount of, e.g., epoxy, glue or Dow-Corning #976V high vacuum grease to hold it in place. For easier handling, attach the base to a magnetic wand. The rod can also be inserted into a 0.7 mm mechanical pencil.

Slowly and carefully insert the gold-colored polyimide tip of the MicroMount™ / MicroLoop™ into the crystal-containing drop, trying to minimize fluid disturbances and motion in the drop. Slide the sample aperture under your crystal, and then carefully remove the crystal + mount from the drop, keeping the crystal centered over the sample aperture if possible. It’s often helpful to brace the magnetic wand or pencil against your other hand or, e.g., a piece of 1/4″ thick foam to help steady the mount and allow smaller, slower motions.

To retrieve crystals that have settled to the bottom of a crystallization plate, try pressing downward to bend the tip of the mount against the bottom so that it slides flat along the bottom. You can often dislodge crystals with gentle pressure.

If using MicroMounts™, you can remove excess liquid by slowly and carefully inserting a size 15 paper wick into the large opening that connects to the sample hole. If you have lots of liquid and you insert the wick too quickly, the crystal will flow with the liquid to the wick. For viscous liquids you may need to “mop up” around the crystal with the wick. Liquid can also be removed by touching a piece of absorbant paper to the back side of the aperture.

Mounted correctly, you should have very little liquid around your crystal. The flash cooling rate will be much faster and the cryoprotectant concentrations required to prevent icing much smaller. If there’s just a thin layer of liquid, it will tend to shatter (like the shell of an egg) on cooling rather than crush your sample.

To minimize ice formation on cooling and to keep your crystal from drying out during handling, first transfer it to a drop of our LV Cryo Oil™ and then mount it. If necessary, remove excess oil by wicking it away, or by flicking it away by gently tapping on the steel rod of the mount.

Flash cool the crystal by your favorite method. We recommend using the hyperquenching protocol of Warkentin et al. (J. Appl. Cryst. (2006) 39, 805-811) , a variant on standard plunge cooling in liquid nitrogen in which you you blow away the cold gas layer that forms above the liquid nitrogen just before you plunge in your sample. You can also use liquid nitrogen that has been vacuum-pumped to near its freezing temperature (to reduce boiling), or (more dangerous) liquid propane, but the hyperquenching method is safer, easier and generally gives the best results.
Comments or suggestions? Please contact us!

使用 Micromounts™ 和 MicroLoops™ 进行高分子晶体学

MicroMounts™ 和 MicroLoops™ 的使用方式与尼龙环安装座大致相同,并且比玻璃纤维和小分子晶体学中传统使用的其他安装方法更容易使用。它们的非磁性不锈钢杆与所有现有的测角仪底座(盖)和安装硬件兼容。我们建议的程序如下。

选择样品孔径尺寸略小于晶体尺寸的安装座。 由于竞争的尼龙环和聚酯薄膜安装座比 MiTeGen 的产品产生更多的背景衍射,因此大多数晶体学家尝试将晶体安装在孔径中间,远离尼龙或聚酯薄膜,并通过横跨环的液体弯月面固定到位。这使得 X 射线束远离强散射的尼龙或聚酯薄膜。但 所有多余的液体都会增强您的背景,并且在冻结时可能会压碎您的晶体。 使用 MiTeGen 的产品,您永远不必担心 X 射线束撞击底座的背景。

安装晶体,使其位于孔径顶部,以尽量减少多余的液体和相关的背景 X 射线散射。选择稍小的孔径也可以更容易地将晶体从溶液中捞出。 你不是“圈”你的水晶,而是舀它! (当然,如果您对此更满意,仍然可以用旧的方式安装晶体,但我们的方法通常会给您提供更好的数据。)

将 MicroMount™ / MicroLoop™ 的不锈钢杆插入 MiTeGen 可重复使用的测角器底座之一,或者 使用少量环氧树脂、胶水或 Dow-Corning #976V 高真空油脂将其固定在标准测角器底座 中到位。为了更方便操作,请将底座连接到磁棒上。该杆还可插入 0.7 毫米自动铅笔。

缓慢而小心地将 MicroMount™ / MicroLoop™ 的金色聚酰亚胺尖端插入含有晶体的液滴中,尽量减少液滴中的流体扰动和运动。 将样品孔滑到晶体下方,然后小心地从水滴中取出晶体 + 安装座,如果可能,将晶体保持在样品孔的中心位置。将磁棒或铅笔靠在另一只手上或者例如一块 1/4 英寸厚的泡沫上通常会很有帮助,以帮助稳定安装座并允许更小、更慢的运动。

要取出已沉降到结晶板底部的晶体,请尝试向下按压以将底座的尖端弯曲到底部,使其沿着底部平坦滑动。 您通常可以通过轻微的压力来去除晶体。

如果使用 MicroMounts™,您可以通过缓慢、小心地将 15 号纸芯 插入连接样品孔的大开口来去除多余的液体。如果您有大量液体并且插入吸芯的速度太快,晶体会随着液体流到吸芯。对于粘性液体,您可能需要用吸芯“擦拭”晶体周围。也可以通过将一张吸水纸接触孔的背面来去除液体。

正确安装后,晶体周围应该只有很少的液体。闪蒸冷却速率将快得多,并且防止结冰所需的冷冻保护剂浓度也会小得多。如果只有一层薄薄的液体,它在冷却时往往会破碎(就像鸡蛋壳一样),而不是压碎样品。

为了最大限度地减少冷却时结冰并防止晶体在处理过程中变干,请先将其转移到一滴 LV Cryo Oil™ 中,然后安装。如有必要,通过吸走多余的油,或轻轻敲击安装座的钢杆将其弹走。

用您最喜欢的方法快速冷却晶体。我们建议使用 Warkentin等人 的超淬火方案 。(J. Appl. Cryst. (2006) 39, 805-811) ,液氮中标准浸入式冷却的一种变体,在浸入样品之前,您需要吹走液氮上方形成的冷气体层。您还可以使用已真空泵至接近其冰点温度的液氮(以减少沸腾)或(更危险的)液态丙烷,但超淬火方法更安全、更容易,并且通常会给出最佳结果。
意见或建议?请联系我们

上海同步辐射装置 (SSRF) –蛋白解决loop环、底座、puck选型指南

上海同步辐射装置 (SSRF) –蛋白解决loop环、底座、puck选型指南

BEAMLINE GUIDE  选型指南
SSRF BL17U1


Shanghai Synchrotron Radiation Facility
Pudong, Shanghai, China
ACTOR Automounter

BL17U at Shanghai Synchrotron Radiation Facility (SSRF) is a PX beamline designed to high resolution and high throughput structural determination of macromolecules and their complexes. The energy range for BL17U is 5-18 keV. The sample changing robot, ACTOR of Rigaku, is installed. The cassette is Uni-Puck and one puck can holds 16 protein crystals.1

上海同步辐射设施
浦东,上海,中国
自动加载器的演员

上海同步辐射设施(SSRF)的BL17U是一种用于高分辨率、高通量大分子及其配合物结构测定的PX光束线。BL17U的能量范围为5- 18kev。安装了Rigaku的ACTOR换样机器人。盒式是Uni-Puck,一个puck可以容纳16个蛋白质晶体

OTHER LINKS

  • Beamline Website
  • Contact Beamline
  • Contact MiTeGen

Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style Uni-Puck, ALS, ACTOR
Mount/Loop Style Any
Rod Length 18mm/SPINE
Base Style B1A-Reusable, B1-R, B3-R, B3S-R, B5-R
*Remote Access of the beamline BL17U at Shanghai Synchrotron Radiation FacilityTo purchase ACTOR pucks, please contact us.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

光束线样品制备套件可根据您的晶体尺寸提供3种孔径尺寸组合,包括:

100水晶安装组件,18毫米引脚,可在以下分类:
20 x双厚度MicroLoops™LD(孔径尺寸分别为10、20、30和50 μm各5个)
40 x双厚度MicroMount™(各10个75,100,150和200 μm孔径尺寸)
20 x双厚度MicroLoops™(孔径大小分别为300、400、500和600 μm各5个)
20 x MicroMeshes™(孔径尺寸分别为10、25、50和25L μm各5个)
安装在100个B5-R可重复使用基地或100个常规基地
包装和存储在5个MiTeGen基础持有人

 

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

Mitegen loop环底座型号 Base Styles Available

Mitegen loop环底座型号 Base Styles Available

B1 (SSRL)

B1A (ALS)

B3 (SSRL)

B3S (ALS)

B5 (SPINE)

快速将环组装到盖子中以进行晶体收获,无需使用胶水或等待环氧树脂干燥。
Quickly assemble loops into caps for crystal harvesting, without using glue or waiting for epoxy to dry.

  • Save Time and Costs

    Eliminate time spent glueing loops into bases.

  • Get Robot Compatible Styles

    Designs available for every all automated beamline sample placement robots

  • Cryogenic and Room Temperature Use

    The bases grip the loops securely at both cryogenic and room temperture

You no longer need to spend time gluing crystal mount/loop pins into your goniometer bases (“caps”) before harvesting your crystals. Gluing and removing glue can be time consuming and messy. Once glued, the loop and base must be used as a single unit. If the loop fails or becomes bent, both loop and base become unusable. If the glue fails during automated handling, the loop and pin can fall out and jam the automounter.


  • 节省时间和成本

    消除将环粘到底座上所花费的时间。

  • 获取机器人兼容样式

    适用于所有自动化光束线样品放置机器人的设计

  • 低温和室温使用

    底座在低温和室温下都能牢固地抓住环

在收获晶体之前,您不再需要花时间将晶体安装/环销粘到测角仪底座(“盖子”)上。粘合和去除胶水可能既耗时又混乱。粘合后,环和底座必须作为一个整体使用。如果环路失效或弯曲,则环路和基座都将无法使用。如果在自动处理过程中胶水失效,环和销可能会脱落并卡住自动安装机。

REUSABLE BASE INSTRUCTIONS FOR USE AND CARE

可重复使用底座的使用和保养说明

For optimum performance and maximum usable life of your reusable bases, we recommend the following procedures:

  1. Make sure the bases are at room temperature before attempting to insert or remove sample mounts. Attempting to insert or remove sample mounts immediately after removal from cryogenic storage can damage the gripping equipment.
  2. Make sure that all parts are clean and dry, and do not use lubricants. Moisture, oils or lubricants will decrease the gripping strength.
  3. Grip the crystal mount’s stainless steel rod firmly using heavy-duty serrated-end tweezers, such as those sold by Mitegen. These minimize rod slip and twisting during insertion and removal.
  4. Insert the stainless steel rod straight down into the center of the post. Avoid tilting the rod to the side or inserting at an angle.
  5. As you insert the rod, rotate the base slowly to help guide the rod into the base and to ensure a strong, uniform hold.
  6. The rod should insert smoothly without excessive force. If insertion is difficult or the grip is not secure, remove the pin and repeat steps 1-3. Do not force the steel rod into the base.
  7. Although these bases are reusable, the will eventually wear out, and may be damaged by improper use. If you cannot obtain a secure grip, either dispose of the base or use epoxy or glue (as with a conventional base.) Do not send loosely held samples to the synchrotron.

为了获得可重复使用底座的最佳性能和最大使用寿命,我们建议执行以下程序:

  1.  在尝试插入或移除样品支架之前,请确保底座处于室温。从低温储存中取出后立即尝试插入或取出样品架可能会损坏夹持设备。
  2. 确保所有部件清洁、干燥,并且 不要使用润滑剂。水分、油或润滑剂会降低握持强度。
  3. 使用重型锯齿状端镊子(例如 Mitegen 出售的镊子)牢牢握住晶体安装座的不锈钢杆 。这些可以最大限度地减少插入和移除过程中杆的滑动和扭曲。
  4. 将不锈钢杆笔直向下插入立柱的中心。 避免将杆倾斜到一侧或以一定角度插入。
  5. 插入杆时,缓慢旋转底座, 以帮助将杆引导到底座中并确保牢固、均匀的固定。
  6. 杆应顺利插入,不得用力过猛。 如果插入困难或抓握不牢固,请拆下销钉并重复步骤 1-3。请勿将钢棒强行插入底座。
  7. 尽管这些底座可以重复使用,但最终会磨损,并且可能因使用不当而损坏。 如果无法牢固抓握,请丢弃底座或使用环氧树脂或胶水(与传统底座一样)。 请勿将松散固定的样品发送到同步加速器。

Technical Specifications for Goniometer Bases B1, B1A, B3, B3S, B4, and B5

Bases
Materials 416 magnetic stainless steel (B1, B1A, B5), copper (B3, B3S) and brass (B4)
Base diameter at bottom B1, B1A, B3, B3S, B5: inside: 9.70 mm; outside: 11.95 mm; B4: 3 mm
Dimensions of tubing capture region diameter: 1.93 mm, length: 5 mm (4 mm for B4).
Central hole diameter 0.81 mm
Central hole bottom – height above goniometer head B1,B3: 0.043″ (1.1 mm)
Height of MicroMount aperture above goniometer head magnet B1: 21 mm for 19 mm pins; B3: 21 mm for 19 mm pins; 19.7 mm for SPINE length pins (as specified in SPINE standard)

测角仪底座 B1、B1A、B3、B3S、B4 和 B5 的技术规格

基地
材料 416磁性不锈钢(B1、B1A、B5)、铜(B3、B3S)和黄铜(B4)
底部直径 B1、B1A、B3、B3S、B5:内侧:9.70 毫米;外侧:11.95 毫米;B4:3毫米
管道捕获区域尺寸 直径:1.93 毫米,长度:5 毫米(B4 为 4 毫米)。
中心孔径 0.81毫米
中心孔底部 – 测角器头上方的高度 B1、B3:0.043 英寸(1.1 毫米)
测角仪头磁体上方 MicroMount 孔径的高度 B1:19 毫米引脚为 21 毫米;B3:19 毫米引脚为 21 毫米;SPINE 长度引脚为 19.7 mm(如 SPINE 标准中指定)

B1A(ALS 型)测角仪底座

用于固定水晶环的磁性底座/盖子。与 ALS 型机器人进样器完全兼容

  • 独特的设计与MicroRT™  毛细管兼容,可用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 款式有 MiTeGen 的专利可重复使用无胶设计或常规需胶设计

B5(SPINE 型)测角仪底座

  • 与MicroRT™毛细管兼容,  用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 设计用于与 ALS 型机器人进样器配合使用。

(正在寻找 GBV SKU?  CryoVials 现已单独出售)

B5(SPINE 型)测角仪底座

用于固定水晶环的磁性底座/盖子。与 SPINE 型机器人进样器完全兼容

  • 独特的设计与MicroRT™  毛细管兼容,可用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 款式有 MiTeGen 的专利可重复使用无胶设计或常规需胶设计

B3(SSRL/SAM 型)测角仪底座

  • 与MicroRT™毛细管兼容,  用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 设计用于与 SSRL/SAM 型机器人进样器配合使用

(正在寻找 GBV SKU?  CryoVials 现已单独出售)

B3(SSRL/SAM 型)测角仪底座

用于固定水晶环的磁性底座/盖子。与 SSRL/SAM 型机器人进样器完全兼容

  • 独特的设计与MicroRT™  毛细管兼容,可用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 款式有 MiTeGen 的专利可重复使用无胶设计或常规需胶设计

B3S(ALS 型)测角仪底座

  • 与MicroRT™毛细管兼容,  用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 设计用于与 ALS 型机器人进样器配合使用。

(正在寻找 GBV SKU?  CryoVials 现已单独出售)

B3S(ALS 型)测角仪底座

用于固定水晶环的磁性底座/盖子。与 ALS 型机器人进样器完全兼容

  • 独特的设计与MicroRT™  毛细管兼容,可用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 款式有 MiTeGen 的专利可重复使用无胶设计或常规需胶设计

 

B4 测角仪底座

底座 B4 是我们的 IUCr 标准“黄铜针”型底座版本,常用于小分子晶体学,经过修改可与MicroMounts™MicroRT™系统一起使用。与其他 3 毫米/1/8 英寸直径底座不同,我们的 B4 具有较小直径的中心孔,可紧密捕获 MicroMounts™ 的不锈钢棒,并具有延伸部分,可捕获 MicroRT™ 毛细管。用于室温和冷冻晶体学应用。

测角仪底座样式

  • 采用最优质的材料精密加工而成,具有耐用性和可靠性。
  • 与MicroRT™毛细管兼容,  用于室温筛选和晶体脱水研究。
  • 可与所有晶体安装座/环一起使用,包括MicroMounts™  、MicroLoops™  、MicroGrippers™  和MicroMesh™  。
  • 唯一接受 19 毫米和 SPINE 长度安装的铜柱底座。
  • 与所有标准冷冻管兼容。

Hampton 带磁力结晶环(ALS型)底座 CrystalCap™ ALS HT

Hampton 带磁力结晶环(ALS型)底座 CrystalCap™ ALS HT

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
  • 2D alphanumeric coding for sample tracking & management
  • Color coded CryoLoop size

DESCRIPTION

The CrystalCap ALS HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS HT attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS HT is compatible with ALS style grippers, auto mounters and sample handlers.

The CrystalCap ALS HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

Note: CrystalCap ALS HT with Mounted CryoLoops are sold without vials. Vials HR4-904 sold separately.

Color Coded Cap…………CryoLoop Size
Red…………………………….0.025-0.05 mm
Green…………………………….0.05-0.1 mm
Blue………………………………..0.2-0.3 mm
Blue/Red………………………….0.3-0.4 mm
Green/Red………………………..0.4-0.5 mm
Yellow/Green……………………..0.7-1.0 mm

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

结晶帽™ALS HT

应用

  • 冰晶术

特征

  • AlS型结晶架
  • 结晶帽ALS HT磁性附着在结晶帽Vial磁力仪基座
  • 可与ALS风格的夹持器、自动装配器和示例处理程序兼容的自由锥形形状
  • 用于样本跟踪和管理的二维字母数字编码
  • 彩色编码冷冻环尺寸

描述

结晶帽ALS HT是一个磁性样品安装(也称为盖子,引脚或测角器底座),为接受ALS型样品安装的冰晶系统设计。结晶帽ALS HT附着在一个磁晶帽和磁测角仪的基座上。水晶帽的顶端接受安装的冷冻环™。

水晶瓶盖是一个1.8毫升的冰瓶,有两个小通风口。将环形磁铁模压到瓶的开口端,以便当盖被放置在瓶中时,环磁铁在低温储存期间将盖保持在瓶上。HR4-904水晶帽瓶底部没有磁铁。

水晶帽ALS HT的无棱镜锥形形状与ALS风格的夹持器、自动装配机和样品处理程序兼容。

水晶帽ALS HT的特点是一个二维字母数字16×16数据矩阵代码在帽子的底部。每个帽子的颜色也编码为Cryoloop大小。

注:水晶帽ALS HT与安装超低温是出售没有小瓶。小瓶HR4-904单独出售.

颜色编码,第.章,冷冻环尺寸
Red..0.025-0.05 mm
Green..0.05-0.1 mm
Blue..0.2-0.3 mm
Blue/Red..0.3-0.4 mm
绿/红.0.4-0.5毫米
黄/绿.0.7至1.0毫米

与下列同步辐射光束线兼容

北美和南美洲
·高级光源,加州伯克利,ALS 4.2.2,ALS 5.0.1,ALS 5.0.2,ALS 5.0.3,ALS 8.2.1,ALS 8.3.1,ALS 11.3.1,ALS 12.2.2,ALS 12.3.2,ALS 12.3.1-APX
*高级光子源,阿贡,伊利诺斯州APS 14-BM-C BioCARS,APS 14-ID-B BioCARS,APS 17-ID IMCA-CAT,APS 19-BM,APS 19-ID,APS 22-BM SER-CAT,APS 22-ID SER-CAT,PS 23-BM-B GA/CA,APS 23-ID-B GA/CA,APS 23-ID-D GA/CA,APS 24-ID-C NE-CAT,APS 24 ID-E NE-CAT,APS 31-ID LR-CAT
·先进微结构和器件中心,巴吞鲁日,路易斯安那州CAMD GCPCC
·康奈尔高能,同步加速器源,伊萨卡,纽约国际象棋A1,国际象棋F1
加拿大光源,加拿大萨斯喀彻温省,CLS 08ID-1,CLS 08B1-1
*巴西同步加速器,光实验室,巴西圣保罗,LNLS D03B-MX1,LNLS W01B-MX2
·斯坦福同步辐射实验室,加州门罗公园,SSRL BL 7-1,SSRL BL 9-1,SSRL BL 9-2,SSRL BL 11-1,SSRL BL 12-2,SSRL BL 14-1

欧洲
·库尔恰托夫同步辐射和纳米技术中心,俄罗斯莫斯科KCSRNT K4.4
·瑞典隆德大学实验室,Max II I 711,Max II I 911-2,Max II I 911-3
*MPG/DESY,德国汉堡,MPGDESY BW6
太阳、圣奥宾、法国太阳PROXIMA 1、太阳PROXIMA 2

亚洲和澳大利亚
·上海同步辐射装置,中国上海SSRF BL17U1
·国家同步辐射研究中心,台湾NSRRC BL13B1,NSRRC BL13C1
·波航加速器实验室,韩国POHANG,PAL 2D,PAL 5C,PAL 7A
·光子工厂,筑波,日本PF BL-5A,PF BL-17A,PF AR-NW12A
超级光子环-8 GeV,日本Spring-8 BL12B2,Spring-8 BL24XU,Spring-8 BL26B1,Spring-8 BL26B2,Spring-8 BL32B2,Spring-8 BL32XU,Spring-8 BL38B1,Spring-8 BL41XU,Spring-8 BL44B2,Spring-8 BL44XU

HR8-200 CrystalCap ALS HT 0.025-0.05 mm CryoLoop – 30 pack
HR8-202 CrystalCap ALS HT 0.05-0.1 mm CryoLoop – 30 pack
HR8-204 CrystalCap ALS HT 0.1-0.2 mm CryoLoop – 30 pack
HR8-206 CrystalCap ALS HT 0.2-0.3 mm CryoLoop – 30 pack
HR8-208 CrystalCap ALS HT 0.3-0.4 mm CryoLoop – 30 pack

RELATED ITEM(S)

Swissci Diaplate 96 Experiment 3.2μl Capacity Dialyser 96孔板 3.2μl 容量

Swissci Diaplate 96 Experiment 3.2μl Capacity Dialyser 96孔板 3.2μl 容量

The disposable Diaplate™ is a 96-well micro-dialysis plate ideal for the desalting of protein from very small volumes up to 3.2μl.

Facts

  • ANSI/SLAS 1-2004 Standard
  • 96 individual membranes
  • 3.2 μl capacity

Key Features

  • Multiple application
  • Complete kit
  • High throughput use

Diaplate 96 Experiment 3.2μl 容量透析器

一次性 Diaplate™ 是一种 96 孔微量透析板,适用于从非常小的体积到 3.2μl 的蛋白质脱盐。

事实

  • ANSI/SLAS 1-2004 标准
  • 96 个单独的膜
  • 3.2 微升容量

主要特征

  • 多次申请
  • 完整套件
  • 高通量使用
  • Description

About

The disposable Diaplate ™ is a 96-well micro-dialysis plate ideal for the dialysis of protein from very small volumes of up to a maximum of 3.2μl.
Each of the 96 wells has a separate regenerated cellulose membrane. ANSI/ SLAS 1-2004 standards provide high-throughput use with robotic dispensing.
The SWISSCI  Diaplate™ comes with a choice of 3, 5, 10, or 30 KDa MWCO membranes. The Diaplate™ kit includes Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron UVP Cover Film, UVXPO evaporation cover plate, and a Sealing Paddle.

 

Applications

 

• Protein and peptide purification/concentration
• Cryo-EM Sample preparation
• Variation of crystallization conditions for crystal growth and Growth of micro-crystals for serial crystallography
• Sample preparation for biophysical assays i.e ThermoFlour®
• Removal of dyes after protein labeling
• Electro-elution of proteins
• Protein and DNA desalting/buffer exchange
• Protein in vitro translation
• Glycoprotein modification and engineering
• Enzyme activity/binding assays/detergent removal

 

Product Specifications

• Simple handling, ready to use, easy sample recovery
• Complies with ANSI/SLAS standards for automation
• Pipetting can be performed manually or using a robot
• Ideal for the desalting of up to 3.2μl protein solution
• No cross-contamination or leakage between wells guaranteed
• 96 individual regenerated cellulose membranes; sulfur and heavy metal-free
• A choice of different MW cut-off membranes
• Protein crystallization applications allow for unique matrix changing
• Low protein binding and high protein retention
• Dialysis complete in 2-4 hours
• Gentle and efficient desalting without disturbing valuable protein complexes

Order Information

Item: Diaplate
Code: W82003; W82005; W82010; W82030.
Details: Pack of 3

  • 描述

关于

一次性 Diaplate™ 是一种 96 孔微量透析板,非常适合透析从非常小体积到最大 3.2μl 的蛋白质。
96 个孔中的每个孔都有单独的再生纤维素膜。ANSI/ SLAS 1-2004 标准提供了机器人点胶的高通量使用。
SWISSCI Diaplate™ 可选择 3、5、10 或 30 KDa MWCO 膜。Diaplate™ 套件包括带 200 微米压力粘合剂垫片的 Diaplate、200 微米 UVP 覆盖膜、UVXPO 蒸发盖板和密封桨。

 

应用

 

• 蛋白质和多肽纯化/浓缩
• 冷冻电镜样品制备
• 晶体生长和微晶生长的结晶条件变化用于连续晶体学 •
生物物理分析样品制备,即 ThermoFlour®
• 蛋白质标记后去除染料
• 电洗脱蛋白质
• 蛋白质和 DNA 脱盐/缓冲液交换
• 蛋白质体外翻译
• 糖蛋白修饰和工程
• 酶活性/结合分析/去垢剂去除

 

产品规格

• 操作简单,随时可用,易于样品回收
• 符合 ANSI/SLAS 自动化标准
• 可手动或使用机器人进行移液
• 最适合 3.2μl 蛋白质溶液脱盐
• 之间无交叉污染或泄漏孔得到保证
• 96 个单独的再生纤维素膜;不含硫和重金属
• 可选择不同的 MW 截止膜
• 蛋白质结晶应用允许独特的基质变化
• 低蛋白质结合和高蛋白质保留
• 透析在 2-4 小时内完成
• 温和高效的脱盐,不会干扰有价值的蛋白质情结

订单信息

项目:  Diaplate
货号: W82003;W82005;W82010;W82030。

DIAPLATE™ Instructions For Use

Preparing the protein
Step 1. Prepare the protein in buffer at the desired concentration and centrifuge (>16,000 g, 10 min) to pellet any unwanted material.
Step 2. Aliquot the protein into 200-μL PCR tubes or similar.
Setting up the DIAPLATE™
Step 3. Peel back and remove the red adhesive cover tape from the 200-μm pressure adhesive spacer. Take note of the
position of A1.
Step 4. Using a multi-channel pipette, load up to a maximum of 3.2 μL of protein into each well
Step 5. Position the 200-μm UV Cover Film onto the 96 wells and check integrity. Please ensure the protective film is
facing up.
Step 6. Use the included sealing paddle to press down over the UV Cover Film to activate and seal the pressure adhesive.
Step 7. Invert the plate and take note of the position of A1. The well positions are now mirrored.
Step 8. Load up to a maximum of 0.35 mL of dialysis solution into each of the wells.
Step 9. Carefully seal the wells with the UVXPO Evaporation Cover Plate by peeling away the red protective film, revealing the adhesive layer. Carefully line up the cover and press down against the wells using the provided paddle
(A) Sealing Plate. Note: Pressure adhesive must be fully activated via paddle. Ensure the evaporation cover is
flush to plate. Alternatively, leave the red protective layer on and use the UVXPO Evaporation Cover Plate as
dust protec-tion during dialysis by placing onto the wells with the red adhesive facing up (recommended if
stepwise dialysis is required) (B) Non Sealed Plate.
Step 10. Place the plate (inverted) in a suitable temperature-controlled incubator for Dialysis/Crystal Growth
DS214.V2
(A) Sealing plate (B) Non Sealed Plate

准备蛋白质
步骤1。在所需浓度的缓冲液中制备蛋白质,然后离心(> 16000 g, 10分钟)以使任何不需要的物质成球。
步骤2。将蛋白分装到200 μ l或类似的PCR管中。
设置diapplate™
步骤3。揭下200 μm压力胶垫片上的红色胶皮。注意
A1的位置。
步骤4。使用多通道移液器,在每孔中最多加载3.2 μL的蛋白质
第5步。将200 μm UV Cover Film放置在96口井上,并检查完整性。请确保保护膜是
面对。
步骤6。使用附带的密封桨压在UV覆盖膜上,以激活和密封压力粘合剂。
步骤7。把钢板倒过来,注意A1的位置。现在,井的位置是镜像的。
步骤8。每个孔中最多装入0.35 mL透析液。
第9步。用UVXPO蒸发盖板小心地密封孔,剥去红色保护膜,露出粘合层。小心地将阀盖对齐,并使用所提供的桨向下压
(A)密封板。注意:压力胶必须通过桨片完全激活。确保蒸发盖是
与盘子平齐。或者,保留红色保护层,使用UVXPO蒸发盖板作为
在透析过程中,通过将红色粘合剂朝上放置在孔上进行防尘保护(建议如果
(B)非密封板。
第10步。将平板(倒置)置于合适的温控培养箱中进行透析/晶体生长
DS214。V2
(A)密封板(B)非密封板

Hampton HR2-122 Crystal Screen Cryo 冷冻删选试剂

Hampton HR2-122 Crystal Screen Cryo 冷冻删选试剂
Hampton蛋白结晶试剂耗材
Hampton Research代理
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APPLICATIONS

  • Primary biased sparse matrix screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules.

应用程序

  • 主要偏置稀疏基质筛选与冷冻蛋白质,可溶性肽,核酸,和水溶性小分子。

FEATURES

  • Developed at Hampton Research
  • Cryo ready screening reagents for cryocrystallography
  • Sparse matrix formula efficiently samples salts, polymers, organics, & pH
  • Tube or Deep Well block format
  • Screen for cryo and crystallization conditions using only one screen

特点

  • 在汉普顿研究所开发的
  • 冷冻冷冻准备筛选试剂
  • 高效样品盐、聚合物、有机物和PH值
  • 管形或深井块格式
  • 只有一个屏幕的冷冻和结晶条件屏幕

DESCRIPTION

Determining initial and optimal cryoprotectant concentration is often a process of trial and error. One must find suitable cryoprotectant concentrations which stabilize the crystal while at the same time combine with the crystallization reagent to form an amorphous glass.

The Crystal Screen Cryo kits removes the guess work from determining the preliminary glycerol concentration required to mix with the crystallization reagent to form an amorphous glass. Crystal Screen Cryo reagents are formulated with the appropriate amount of glycerol required to form an amorphous glass with each unique reagent composition. Crystals obtained in Crystal Screen Cryo kits will already have a suitable concentration of glycerol in the reagent. This avoids exhaustive empirical searches for preliminary cryoprotectant conditions. From the preliminary cryoprotectant conditions, cryoprotectant concentration can be optimized to minimize mosaic spread and maximize diffraction resolution.

Crystal Screen Cryo kits are biased sparse matrices designed for rapid screening of crystallization conditions of biological macromolecules in the presence of glycerol, the most frequently utilized cryoprotectant. The Crystal Screen Cryo kits utilize the original Crystal Screen™ and Crystal Screen 2™ protocol optimized to include the appropriate concentration of glycerol required to form an amorphous glass at 100°K. The primary screen variables are salt, pH, precipitant (salts, polymers, volatile organics, and non-volatile organics), and cryoprotectant concentration. The screen is a straightforward, effective, and practical kit for determining preliminary crystallization conditions and provides a good starting point for finding suitable cryoprotectant conditions for macromolecular crystals grown in a wide range of reagents. Crystal Screen Cryo is also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.

Crystal Screen Cryo contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991 and refined for cryo by Garman in 1996.

Crystal Screen 2 Cryo contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994 and refined for cryo by Snell et al in 2002.

Crystal Screen Cryo HT contains 1 ml each of reagents 1-48 from Crystal Screen Cryo and 48 reagents from Crystal Screen 2 Cryo in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers.

描述

测定初始和最佳低温保护剂浓度通常是一个反复试验和错误的过程。必须找到合适的低温保护剂浓度,稳定晶体,同时与结晶试剂结合形成非晶玻璃。

晶屏冷冻包去除了猜测工作,以确定所需的初步甘油浓度与结晶试剂混合形成非晶态玻璃。晶屏冷冻试剂是用适当量的甘油制备的,以形成一个无定形的玻璃和每个独特的试剂组合。在晶体屏幕上获得的冰柜中的晶体在试剂中已经有了适当浓度的甘油。这避免了对初步低温保护剂条件的详尽的实证研究。从初步的低温保护剂条件出发,可以优化低温保护剂浓度,以最小化马赛克扩散和最大化衍射分辨率。

晶体筛冰柜是偏置稀疏矩阵,用于快速筛选甘油(最常用的低温保护剂)下生物大分子的结晶条件。水晶屏冰柜利用原来的水晶屏和2晶屏协议进行优化,以包括在100°K时形成非晶玻璃所需的甘油的适当浓度。主要的筛选变量是盐、PH值、沉淀剂(盐、聚合物、挥发性有机物和非挥发性有机物)和低温保护剂浓度。该筛是一个简单、有效和实用的工具包,用于确定初步结晶条件,并提供了一个良好的起点,以寻找适合的冷冻保护条件的大分子晶体生长在广泛的试剂。在测定大分子在大量沉淀剂和PH中的溶解度时,晶体筛冷冻也是有效的。

水晶屏冷冻含有50种独特的试剂,每一种有10毫升,它是根据Jancari和Kim于1991年首次描述的稀疏的基质配方,1996年加曼对其进行了精炼。

水晶屏2冷冻含有48种独特的试剂,每一种10毫升,是根据1994年由库德尼等人首次描述的配方,2002年由斯内尔等人提炼而成。

水晶屏冰柜中含有1毫升的试剂1-48从水晶屏冰柜和48毫升的试剂从水晶屏2冰柜以单一深井块格式。

现成的试剂是无菌过滤和使用最高纯度盐,聚合物,有机物和缓冲剂的超纯1型水编制。

HR2-122 Crystal Screen Cryo 10 ml, tube format
HR2-121 Crystal Screen 2 Cryo 10 ml, tube format
HR2-133 Crystal Screen Cryo HT 1 ml, Deep Well block format

Hampton 9 Well Glass Plate & Sandwich Box Setup

Hampton 9 Well Glass Plate & Sandwich Box Setup

APPLICATIONS

  • Sitting drop crystallization
  • Seeding
  • Heavy atom soaks
  • Cryo transfers
  • Large drops to produce large crystals for neutron diffraction studies

应用程序
静滴结晶
播种
沉重的原子泡
低温转移
用于中子衍射研究的大液滴产生大晶体

FEATURES 特性

  • Siliconized, 9 well glass plates
  • Concave wells
  • Polystyrene box and support

硅化,9孔玻璃板
凹井
聚苯乙烯盒和支架

DESCRIPTION

When setting up a Sandwich Box, one pipets 25 ml of crystallization reagent or dehydrant into the bottom of the Sandwich Box, then places a support into the box. The 9 well depression plate is placed upon the support. Drops of sample and reagent are pipetted into the depressions and the lid of the Sandwich Box is sealed closed with vacuum grease.

Why Sandwich Boxes? They allow one to use very, very large drops. They are optically superior to plastic plates and glass slides, and offer different equilibration kinetics than other crystallization plates. Each of the Sandwich Box components can be washed and reused so there is little waste with the Sandwich Box Setup. These plates can be used during screening and optimization, but are best suited for final optimization and production of crystals. Since the glass plates can be removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual components so one can customize the system to meet their needs. Square cover slides can be used to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box Setup consists of a square plastic box (4 5/16” x 4 5/16” x 1 1/8”), a polystyrene plate support, and a siliconized, glass plate (4” L x 3 3/8” W (100 mm x 85 mm)) with nine concave depressions (7/8” O.D. x 1/4” D (22 mm x 7 mm)).

Drop sizes up to 800 microliters are possible with the Sandwich Box Setup.

描述
当设置一个三明治盒,一个移液管25毫升的结晶试剂或脱水剂到三明治盒的底部,然后放置一个支撑在盒子里。9孔压板放置在支架上。样品和试剂滴入凹陷处,三明治盒的盖子用真空润滑脂密封。

为什么是三明治盒?它们允许人们使用非常非常大的水滴。它们在光学上优于塑料板和玻璃载玻片,并提供不同于其他晶化板的平衡动力学。每个三明治盒组件都可以清洗和重复使用,因此三明治盒设置几乎没有浪费。这些板可用于筛选和优化,但最适合最终优化和生产晶体。由于玻璃板可以从盒子上拆下来,水晶安装很方便。硅化9孔凹板也适用于重原子浸泡,冷溶液稀释和转移,和播种实验,其中涉及一系列稀释。三明治盒设置可作为一个完整的集合或作为单独的组件,因此可以自定义系统,以满足他们的需求。方形盖滑块可用于密封单个储层,但通常不用于结晶装置。三明治盒设置包括一个方形塑料盒(4 5/16“x 4 5/16“x 1 1/8“),一个聚苯乙烯板支撑,和一个硅化,玻璃板(4“长x 3 3/8“宽(100毫米x 85毫米))与九个凹凹陷(7/8“od x 1/4“D(22毫米x 7毫米))。

滴大小可达800微升与三明治盒设置。

RELATED ITEM(S)

REFERENCES 参考文献

1. In situ X-ray crystallography. A. McPherson. J. Appl. Cryst. (2000). 33, 397-400.

2. Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum. Kovalevsky, A. Y., Hanson, B. L., Seaver, S., Fisher, S. Z., Mustyakimov, M. & Langan, P. (2011). Acta Cryst. F67, 283-286.

3. Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments. Acta Crystallographica Section D Structural Biology. 75. 10.1107/S2059798319012695.   Andersson, Rebecka & Safari, Cecilia & Båth, Petra & Bosman, Robert & Shilova, Anastasya & Dahl, Peter & Ghosh, Swagatha & Dunge, Andreas & Kjeldsen-Jensen, Rasmus & Nan, Jie & Shoeman, Robert & Kloos, Marco & Doak, R. & Mueller, Uwe & Neutze, Richard & Brändén, Gisela. (2019).

Hampton HR6-104 0.1毫米50号玻璃毛细管

Hampton HR6-104 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用

X射线数据采集

液-液扩散结晶

凝胶针状结晶

特征

50号硼硅玻璃

壁厚 10微米

描述

硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。

请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。

0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。

Hampton HR6-104 0.1毫米50号玻璃毛细管 其他相关产品:

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm