Swissci Diaplate 96 Experiment 3.2μl Capacity Dialyser 96孔板 3.2μl 容量

Swissci Diaplate 96 Experiment 3.2μl Capacity Dialyser 96孔板 3.2μl 容量

The disposable Diaplate™ is a 96-well micro-dialysis plate ideal for the desalting of protein from very small volumes up to 3.2μl.

Facts

  • ANSI/SLAS 1-2004 Standard
  • 96 individual membranes
  • 3.2 μl capacity

Key Features

  • Multiple application
  • Complete kit
  • High throughput use

Diaplate 96 Experiment 3.2μl 容量透析器

一次性 Diaplate™ 是一种 96 孔微量透析板,适用于从非常小的体积到 3.2μl 的蛋白质脱盐。

事实

  • ANSI/SLAS 1-2004 标准
  • 96 个单独的膜
  • 3.2 微升容量

主要特征

  • 多次申请
  • 完整套件
  • 高通量使用
  • Description

About

The disposable Diaplate ™ is a 96-well micro-dialysis plate ideal for the dialysis of protein from very small volumes of up to a maximum of 3.2μl.
Each of the 96 wells has a separate regenerated cellulose membrane. ANSI/ SLAS 1-2004 standards provide high-throughput use with robotic dispensing.
The SWISSCI  Diaplate™ comes with a choice of 3, 5, 10, or 30 KDa MWCO membranes. The Diaplate™ kit includes Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron UVP Cover Film, UVXPO evaporation cover plate, and a Sealing Paddle.

 

Applications

 

• Protein and peptide purification/concentration
• Cryo-EM Sample preparation
• Variation of crystallization conditions for crystal growth and Growth of micro-crystals for serial crystallography
• Sample preparation for biophysical assays i.e ThermoFlour®
• Removal of dyes after protein labeling
• Electro-elution of proteins
• Protein and DNA desalting/buffer exchange
• Protein in vitro translation
• Glycoprotein modification and engineering
• Enzyme activity/binding assays/detergent removal

 

Product Specifications

• Simple handling, ready to use, easy sample recovery
• Complies with ANSI/SLAS standards for automation
• Pipetting can be performed manually or using a robot
• Ideal for the desalting of up to 3.2μl protein solution
• No cross-contamination or leakage between wells guaranteed
• 96 individual regenerated cellulose membranes; sulfur and heavy metal-free
• A choice of different MW cut-off membranes
• Protein crystallization applications allow for unique matrix changing
• Low protein binding and high protein retention
• Dialysis complete in 2-4 hours
• Gentle and efficient desalting without disturbing valuable protein complexes

Order Information

Item: Diaplate
Code: W82003; W82005; W82010; W82030.
Details: Pack of 3

  • 描述

关于

一次性 Diaplate™ 是一种 96 孔微量透析板,非常适合透析从非常小体积到最大 3.2μl 的蛋白质。
96 个孔中的每个孔都有单独的再生纤维素膜。ANSI/ SLAS 1-2004 标准提供了机器人点胶的高通量使用。
SWISSCI Diaplate™ 可选择 3、5、10 或 30 KDa MWCO 膜。Diaplate™ 套件包括带 200 微米压力粘合剂垫片的 Diaplate、200 微米 UVP 覆盖膜、UVXPO 蒸发盖板和密封桨。

 

应用

 

• 蛋白质和多肽纯化/浓缩
• 冷冻电镜样品制备
• 晶体生长和微晶生长的结晶条件变化用于连续晶体学 •
生物物理分析样品制备,即 ThermoFlour®
• 蛋白质标记后去除染料
• 电洗脱蛋白质
• 蛋白质和 DNA 脱盐/缓冲液交换
• 蛋白质体外翻译
• 糖蛋白修饰和工程
• 酶活性/结合分析/去垢剂去除

 

产品规格

• 操作简单,随时可用,易于样品回收
• 符合 ANSI/SLAS 自动化标准
• 可手动或使用机器人进行移液
• 最适合 3.2μl 蛋白质溶液脱盐
• 之间无交叉污染或泄漏孔得到保证
• 96 个单独的再生纤维素膜;不含硫和重金属
• 可选择不同的 MW 截止膜
• 蛋白质结晶应用允许独特的基质变化
• 低蛋白质结合和高蛋白质保留
• 透析在 2-4 小时内完成
• 温和高效的脱盐,不会干扰有价值的蛋白质情结

订单信息

项目:  Diaplate
货号: W82003;W82005;W82010;W82030。

DIAPLATE™ Instructions For Use

Preparing the protein
Step 1. Prepare the protein in buffer at the desired concentration and centrifuge (>16,000 g, 10 min) to pellet any unwanted material.
Step 2. Aliquot the protein into 200-μL PCR tubes or similar.
Setting up the DIAPLATE™
Step 3. Peel back and remove the red adhesive cover tape from the 200-μm pressure adhesive spacer. Take note of the
position of A1.
Step 4. Using a multi-channel pipette, load up to a maximum of 3.2 μL of protein into each well
Step 5. Position the 200-μm UV Cover Film onto the 96 wells and check integrity. Please ensure the protective film is
facing up.
Step 6. Use the included sealing paddle to press down over the UV Cover Film to activate and seal the pressure adhesive.
Step 7. Invert the plate and take note of the position of A1. The well positions are now mirrored.
Step 8. Load up to a maximum of 0.35 mL of dialysis solution into each of the wells.
Step 9. Carefully seal the wells with the UVXPO Evaporation Cover Plate by peeling away the red protective film, revealing the adhesive layer. Carefully line up the cover and press down against the wells using the provided paddle
(A) Sealing Plate. Note: Pressure adhesive must be fully activated via paddle. Ensure the evaporation cover is
flush to plate. Alternatively, leave the red protective layer on and use the UVXPO Evaporation Cover Plate as
dust protec-tion during dialysis by placing onto the wells with the red adhesive facing up (recommended if
stepwise dialysis is required) (B) Non Sealed Plate.
Step 10. Place the plate (inverted) in a suitable temperature-controlled incubator for Dialysis/Crystal Growth
DS214.V2
(A) Sealing plate (B) Non Sealed Plate

准备蛋白质
步骤1。在所需浓度的缓冲液中制备蛋白质,然后离心(> 16000 g, 10分钟)以使任何不需要的物质成球。
步骤2。将蛋白分装到200 μ l或类似的PCR管中。
设置diapplate™
步骤3。揭下200 μm压力胶垫片上的红色胶皮。注意
A1的位置。
步骤4。使用多通道移液器,在每孔中最多加载3.2 μL的蛋白质
第5步。将200 μm UV Cover Film放置在96口井上,并检查完整性。请确保保护膜是
面对。
步骤6。使用附带的密封桨压在UV覆盖膜上,以激活和密封压力粘合剂。
步骤7。把钢板倒过来,注意A1的位置。现在,井的位置是镜像的。
步骤8。每个孔中最多装入0.35 mL透析液。
第9步。用UVXPO蒸发盖板小心地密封孔,剥去红色保护膜,露出粘合层。小心地将阀盖对齐,并使用所提供的桨向下压
(A)密封板。注意:压力胶必须通过桨片完全激活。确保蒸发盖是
与盘子平齐。或者,保留红色保护层,使用UVXPO蒸发盖板作为
在透析过程中,通过将红色粘合剂朝上放置在孔上进行防尘保护(建议如果
(B)非密封板。
第10步。将平板(倒置)置于合适的温控培养箱中进行透析/晶体生长
DS214。V2
(A)密封板(B)非密封板

Hampton 9 Well Glass Plate & Sandwich Box Setup

Hampton 9 Well Glass Plate & Sandwich Box Setup

APPLICATIONS

  • Sitting drop crystallization
  • Seeding
  • Heavy atom soaks
  • Cryo transfers
  • Large drops to produce large crystals for neutron diffraction studies

应用程序
静滴结晶
播种
沉重的原子泡
低温转移
用于中子衍射研究的大液滴产生大晶体

FEATURES 特性

  • Siliconized, 9 well glass plates
  • Concave wells
  • Polystyrene box and support

硅化,9孔玻璃板
凹井
聚苯乙烯盒和支架

DESCRIPTION

When setting up a Sandwich Box, one pipets 25 ml of crystallization reagent or dehydrant into the bottom of the Sandwich Box, then places a support into the box. The 9 well depression plate is placed upon the support. Drops of sample and reagent are pipetted into the depressions and the lid of the Sandwich Box is sealed closed with vacuum grease.

Why Sandwich Boxes? They allow one to use very, very large drops. They are optically superior to plastic plates and glass slides, and offer different equilibration kinetics than other crystallization plates. Each of the Sandwich Box components can be washed and reused so there is little waste with the Sandwich Box Setup. These plates can be used during screening and optimization, but are best suited for final optimization and production of crystals. Since the glass plates can be removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual components so one can customize the system to meet their needs. Square cover slides can be used to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box Setup consists of a square plastic box (4 5/16” x 4 5/16” x 1 1/8”), a polystyrene plate support, and a siliconized, glass plate (4” L x 3 3/8” W (100 mm x 85 mm)) with nine concave depressions (7/8” O.D. x 1/4” D (22 mm x 7 mm)).

Drop sizes up to 800 microliters are possible with the Sandwich Box Setup.

描述
当设置一个三明治盒,一个移液管25毫升的结晶试剂或脱水剂到三明治盒的底部,然后放置一个支撑在盒子里。9孔压板放置在支架上。样品和试剂滴入凹陷处,三明治盒的盖子用真空润滑脂密封。

为什么是三明治盒?它们允许人们使用非常非常大的水滴。它们在光学上优于塑料板和玻璃载玻片,并提供不同于其他晶化板的平衡动力学。每个三明治盒组件都可以清洗和重复使用,因此三明治盒设置几乎没有浪费。这些板可用于筛选和优化,但最适合最终优化和生产晶体。由于玻璃板可以从盒子上拆下来,水晶安装很方便。硅化9孔凹板也适用于重原子浸泡,冷溶液稀释和转移,和播种实验,其中涉及一系列稀释。三明治盒设置可作为一个完整的集合或作为单独的组件,因此可以自定义系统,以满足他们的需求。方形盖滑块可用于密封单个储层,但通常不用于结晶装置。三明治盒设置包括一个方形塑料盒(4 5/16“x 4 5/16“x 1 1/8“),一个聚苯乙烯板支撑,和一个硅化,玻璃板(4“长x 3 3/8“宽(100毫米x 85毫米))与九个凹凹陷(7/8“od x 1/4“D(22毫米x 7毫米))。

滴大小可达800微升与三明治盒设置。

RELATED ITEM(S)

REFERENCES 参考文献

1. In situ X-ray crystallography. A. McPherson. J. Appl. Cryst. (2000). 33, 397-400.

2. Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum. Kovalevsky, A. Y., Hanson, B. L., Seaver, S., Fisher, S. Z., Mustyakimov, M. & Langan, P. (2011). Acta Cryst. F67, 283-286.

3. Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments. Acta Crystallographica Section D Structural Biology. 75. 10.1107/S2059798319012695.   Andersson, Rebecka & Safari, Cecilia & Båth, Petra & Bosman, Robert & Shilova, Anastasya & Dahl, Peter & Ghosh, Swagatha & Dunge, Andreas & Kjeldsen-Jensen, Rasmus & Nan, Jie & Shoeman, Robert & Kloos, Marco & Doak, R. & Mueller, Uwe & Neutze, Richard & Brändén, Gisela. (2019).

Hampton HR6-104 0.1毫米50号玻璃毛细管

Hampton HR6-104 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用

X射线数据采集

液-液扩散结晶

凝胶针状结晶

特征

50号硼硅玻璃

壁厚 10微米

描述

硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。

请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。

0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。

Hampton HR6-104 0.1毫米50号玻璃毛细管 其他相关产品:

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

Hampton 50号毛细管Glass Number 50 Capillaries产品详细说明

Hampton 50号毛细管Glass Number 50 Capillaries

Glass Number 50 Capillaries

应用:

  X-ray data collection

  Liquid-liquid diffusion crystallization

  Gel acupuncture crystallization

Features

  Glass Number 50 (borosilicate)

  Thin walled – 10 micron

      上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Description 描述
Glass Number 50 (borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.

The Diameter is measured 40 mm from the closed end. The tolerances are as follows

0.1 mm -0.075 / +0.075 mm
0.2 mm -0.050 / +0.050 mm
0.3 mm -0.050 / +0.100 mm
0.4 mm -0.100 / +0.100 mm
0.5 mm -0.100 / +0.100 mm
0.6 mm -0.100 / +0.100 mm
0.7 mm -0.150 / +0.150 mm
0.8 mm -0.150 / +0.150 mm
0.9 mm -0.150 / +0.150 mm
1.0 mm -0.150 / +0.250 mm
1.5 mm -0.250 / +0.250 mm
2.0 mm -0.250 / +0.250 mm

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

上海金畔生物科技有限公司(www.jinpanbio.cn)提供生命科学研究领域系列产品,包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;WAKO日本和光纯药、修饰性PEG(Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、小鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

Hampton Santovac Cryo Oil替代HR2-643 Parabar10312

Hampton Santovac Cryo Oil替代HR2-643 Parabar10312

APPLICATIONS

  • Oil based cryoprotectant

应用
油基冷冻保护剂

FEATURES

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant

特征
低粘度
低表面张力
可溶性蛋白和膜蛋白晶体的冷冻保护剂
与大多数溶剂不混溶
无毒、非渗透性冷冻保护剂

DESCRIPTION

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

描述
在冷冻晶体学期间用于在晶体安装在冷冻环中后置换和减少晶体上的水(母液、试剂)的量。用油涂覆晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)对晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将镶嵌好的晶体浸入油中以置换地表水。或在安装晶体之前向结晶滴中添加少量 Santovac。添加 Santovac 后,使用 Mounted Cryoloop 安装晶体。从液滴中取出已安装的晶体,Santovac 将涂覆已安装的晶体。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种具有极低蒸气压和低反应性的五环聚苯醚 (PPE)。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全且无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

HR2-861 Santovac Cryo Oil 1 mL

RELATED ITEM(S)

REFERENCES

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势:货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

 Optimize Reagents Optimize – Cryoprotectants Santovac Cryo Oil

APPLICATIONS

  • Oil based cryoprotectant

应用
油基防冻剂

FEATURES

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant

特征
低粘度
低表面张力
可溶性和膜蛋白晶体的冷冻保护剂
不与大多数溶剂混溶
无毒、非渗透性冷冻保护剂

DESCRIPTION

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

HR2-861  Santovac Cryo Oil 1 mL

描述
在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气(氧气)向晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后,使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶,Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE),具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

HR2-861 Santovac 冷冻油 1 毫升

RELATED ITEM(S) 相关项目
CryoPro

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

REFERENCES  参考文献
1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

Rigaku–Tubes, Wizard Classic 1

Rigaku–Tubes, Wizard Classic 1

Tubes, Wizard Classic 1

  • Tubes, Wizard Classic 3

  • Tubes, Wizard Classic 2

  • Tubes, Wizard Classic 1

  • Wizard Classic 4, condition 48.

  • Wizard Classic 4, condition 47.

  • Wizard Classic 4, condition 46.

  • Wizard Classic 4, condition 45.

  • Wizard Classic 4, condition 44.

  • Wizard Classic 4, condition 43.

Rigaku Reagents蛋白结晶试剂耗材

Rigaku Reagents蛋白结晶试剂耗材

上海金畔生物代理Rigaku品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Rigaku官网或者咨询我们获取更多相关Rigaku品牌产品信息。

Rigaku Reagents是蛋白质科学的提供商,为蛋白质研究人员提供蛋白质研究试剂盒、试剂、储液、和相关配件。

作为创新者,Rigaku Reagents团队不断开发蛋白质研究工具,以加速蛋白质研究发现。

Rigaku Reagents蛋白结晶部分相关产品:

货号 产品名称
1009530 Wizard Classic 1
1009531 Wizard Classic 2
1009533 Wizard Classic 3
1009534 Wizard Classic 4
1009532 Wizard Classic 1 & 2
1009535 Wizard Classic 3 & 4

Molecular Dimensions 蛋白结晶培养箱(台式)

Molecular Dimensions 蛋白结晶培养箱(台式)

Bench-Top Incubators

A simple, cost-effective bench top solution for crystal growth and temperature screening.

Key Features:

  • Ultra-low vibration Peltier-based. No compressors, no CFCs. Energy-efficient
  • Temperature range from 4.0°C to 70.0°C, with an accuracy ± 0.5°C.
  • Choose between fully programmable or non- programmable controls.
  • 27.5L, 55 L or new 100L volumes
  • Timer with alarm and Auto-Off
  • Electronic calibration and power failure protection
  • RS232 serial port interface
  • 12-month warranty, UL, CSA and CE listed

Plate Capacity (approx max):
27.5 L model: 60 x SBS, 25 x XRL/Linbro
55.0 L model: 140 x SBS, 58 x XRL/Linbro
100  L model: 250 x SBS, 112 x XRL/Linbro

“Nicely built product helpful in crystallization of proteins. It is light weight and have ergonomic design. Peltier based cooling keeps it vibration free.”
Suman Kumar Mandal, Shiv Nadar University, India

用于晶体生长和温度筛选的简单、经济高效的台式解决方案。

主要特点:

  • 基于 Peltier 的超低振动。没有压缩机,没有 CFC。高效节能
  • 温度范围为 4.0°C 至 70.0°C,精度为 ± 0.5°C。
  • 在完全可编程或非可编程控制之间进行选择。
  • 27.5L、55L 或新的 100L 容积
  • 带闹钟和自动关闭功能的定时器
  • 电子校准和断电保护
  • RS232串口接口
  • 12 个月保修,通过 UL、CSA 和 CE 认证

印版容量(大约最大):
27.5 L 型号:60 x SBS、25 x XRL/Linbro
55.0 L 型号:140 x SBS、58 x XRL/Linbro
100 L 型号:250 x SBS、112 x XRL/

Linbro有助于蛋白质结晶。重量轻,符合人体工程学设计。基于 Peltier 的冷却使其无振动。
Suman Kumar Mandal,印度希夫纳达尔大学

Product # Product Name
MD5-600 27.5L Bench Top Incubator (Non-programmable)
MD5-601 27.5L Bench Top Incubator (Fully-programmable)
MD5-602 55L Bench Top Incubator (Non-programmable)
MD5-603 55L Bench Top Incubator (Fully-programmable)
MD5-604 100L Bench Top Incubator (Non-programmable)
MD5-605 100L Bench Top Incubator (Fully-programmable)
MD5-606 Bench Top Incubator Stacker
产品名称 单位尺寸
MD5-600 27.5L 台式培养箱(不可编程)
MD5-601 27.5L 台式培养箱(完全可编程)
MD5-602 55L 台式培养箱(不可编程)
MD5-603 55L 台式培养箱(完全可编程)
MD5-604 100L 台式培养箱(不可编程)
MD5-605 100L 台式培养箱(完全可编程)
MD5-606 台式培养箱堆垛机

使用盐作为结晶试剂

Cryosalts   使用盐作为结晶试剂

Using salt as a crystallization reagent?

Many salts are cryosalts, including malonate, formate, citrate, tartrate, acetate, Tacsimate and other organic acids, ammonium sulfate (>3.5 M), lithium sulfate, lithium chloride and other alkylammonium salts. For cryo try increasing your salt concentration by 20%. (Cryosalts: suppression of ice formation in macromolecular crystallography, K. A. Rubinson et al, Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587.

冰晶盐
使用盐作为结晶试剂?

很多盐是冰晶盐,包括丙二酸盐、甲酸盐、柠檬酸盐、酒石酸盐、乙酸盐、Tacsimate和其他有机酸、硫酸铵(>3.5 M)、硫酸锂、氯化锂和其他烷基铵盐。 对于冷冻,尝试将盐浓度提高 20%。 (冷冻盐:大分子晶体学中冰形成的抑制,K. A. Rubinson 等人,Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587。