Hampton Santovac Cryo Oil替代HR2-643 Parabar10312

Hampton Santovac Cryo Oil替代HR2-643 Parabar10312

APPLICATIONS

  • Oil based cryoprotectant

应用
油基冷冻保护剂

FEATURES

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant

特征
低粘度
低表面张力
可溶性蛋白和膜蛋白晶体的冷冻保护剂
与大多数溶剂不混溶
无毒、非渗透性冷冻保护剂

DESCRIPTION

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

描述
在冷冻晶体学期间用于在晶体安装在冷冻环中后置换和减少晶体上的水(母液、试剂)的量。用油涂覆晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)对晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将镶嵌好的晶体浸入油中以置换地表水。或在安装晶体之前向结晶滴中添加少量 Santovac。添加 Santovac 后,使用 Mounted Cryoloop 安装晶体。从液滴中取出已安装的晶体,Santovac 将涂覆已安装的晶体。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种具有极低蒸气压和低反应性的五环聚苯醚 (PPE)。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全且无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

HR2-861 Santovac Cryo Oil 1 mL

RELATED ITEM(S)

REFERENCES

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势:货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

 Optimize Reagents Optimize – Cryoprotectants Santovac Cryo Oil

APPLICATIONS

  • Oil based cryoprotectant

应用
油基防冻剂

FEATURES

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant

特征
低粘度
低表面张力
可溶性和膜蛋白晶体的冷冻保护剂
不与大多数溶剂混溶
无毒、非渗透性冷冻保护剂

DESCRIPTION

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

HR2-861  Santovac Cryo Oil 1 mL

描述
在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气(氧气)向晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后,使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶,Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE),具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

HR2-861 Santovac 冷冻油 1 毫升

RELATED ITEM(S) 相关项目
CryoPro

Hampton HR2-861 Santovac Cryo Oil 油基防冻剂

REFERENCES  参考文献
1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

Rigaku–Tubes, Wizard Classic 1

Rigaku–Tubes, Wizard Classic 1

Tubes, Wizard Classic 1

  • Tubes, Wizard Classic 3

  • Tubes, Wizard Classic 2

  • Tubes, Wizard Classic 1

  • Wizard Classic 4, condition 48.

  • Wizard Classic 4, condition 47.

  • Wizard Classic 4, condition 46.

  • Wizard Classic 4, condition 45.

  • Wizard Classic 4, condition 44.

  • Wizard Classic 4, condition 43.

Rigaku Reagents蛋白结晶试剂耗材

Rigaku Reagents蛋白结晶试剂耗材

上海金畔生物代理Rigaku品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Rigaku官网或者咨询我们获取更多相关Rigaku品牌产品信息。

Rigaku Reagents是蛋白质科学的提供商,为蛋白质研究人员提供蛋白质研究试剂盒、试剂、储液、和相关配件。

作为创新者,Rigaku Reagents团队不断开发蛋白质研究工具,以加速蛋白质研究发现。

Rigaku Reagents蛋白结晶部分相关产品:

货号 产品名称
1009530 Wizard Classic 1
1009531 Wizard Classic 2
1009533 Wizard Classic 3
1009534 Wizard Classic 4
1009532 Wizard Classic 1 & 2
1009535 Wizard Classic 3 & 4

Molecular Dimensions 蛋白结晶培养箱(台式)

Molecular Dimensions 蛋白结晶培养箱(台式)

Bench-Top Incubators

A simple, cost-effective bench top solution for crystal growth and temperature screening.

Key Features:

  • Ultra-low vibration Peltier-based. No compressors, no CFCs. Energy-efficient
  • Temperature range from 4.0°C to 70.0°C, with an accuracy ± 0.5°C.
  • Choose between fully programmable or non- programmable controls.
  • 27.5L, 55 L or new 100L volumes
  • Timer with alarm and Auto-Off
  • Electronic calibration and power failure protection
  • RS232 serial port interface
  • 12-month warranty, UL, CSA and CE listed

Plate Capacity (approx max):
27.5 L model: 60 x SBS, 25 x XRL/Linbro
55.0 L model: 140 x SBS, 58 x XRL/Linbro
100  L model: 250 x SBS, 112 x XRL/Linbro

“Nicely built product helpful in crystallization of proteins. It is light weight and have ergonomic design. Peltier based cooling keeps it vibration free.”
Suman Kumar Mandal, Shiv Nadar University, India

用于晶体生长和温度筛选的简单、经济高效的台式解决方案。

主要特点:

  • 基于 Peltier 的超低振动。没有压缩机,没有 CFC。高效节能
  • 温度范围为 4.0°C 至 70.0°C,精度为 ± 0.5°C。
  • 在完全可编程或非可编程控制之间进行选择。
  • 27.5L、55L 或新的 100L 容积
  • 带闹钟和自动关闭功能的定时器
  • 电子校准和断电保护
  • RS232串口接口
  • 12 个月保修,通过 UL、CSA 和 CE 认证

印版容量(大约最大):
27.5 L 型号:60 x SBS、25 x XRL/Linbro
55.0 L 型号:140 x SBS、58 x XRL/Linbro
100 L 型号:250 x SBS、112 x XRL/

Linbro有助于蛋白质结晶。重量轻,符合人体工程学设计。基于 Peltier 的冷却使其无振动。
Suman Kumar Mandal,印度希夫纳达尔大学

Product # Product Name
MD5-600 27.5L Bench Top Incubator (Non-programmable)
MD5-601 27.5L Bench Top Incubator (Fully-programmable)
MD5-602 55L Bench Top Incubator (Non-programmable)
MD5-603 55L Bench Top Incubator (Fully-programmable)
MD5-604 100L Bench Top Incubator (Non-programmable)
MD5-605 100L Bench Top Incubator (Fully-programmable)
MD5-606 Bench Top Incubator Stacker
产品名称 单位尺寸
MD5-600 27.5L 台式培养箱(不可编程)
MD5-601 27.5L 台式培养箱(完全可编程)
MD5-602 55L 台式培养箱(不可编程)
MD5-603 55L 台式培养箱(完全可编程)
MD5-604 100L 台式培养箱(不可编程)
MD5-605 100L 台式培养箱(完全可编程)
MD5-606 台式培养箱堆垛机

使用盐作为结晶试剂

Cryosalts   使用盐作为结晶试剂

Using salt as a crystallization reagent?

Many salts are cryosalts, including malonate, formate, citrate, tartrate, acetate, Tacsimate and other organic acids, ammonium sulfate (>3.5 M), lithium sulfate, lithium chloride and other alkylammonium salts. For cryo try increasing your salt concentration by 20%. (Cryosalts: suppression of ice formation in macromolecular crystallography, K. A. Rubinson et al, Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587.

冰晶盐
使用盐作为结晶试剂?

很多盐是冰晶盐,包括丙二酸盐、甲酸盐、柠檬酸盐、酒石酸盐、乙酸盐、Tacsimate和其他有机酸、硫酸铵(>3.5 M)、硫酸锂、氯化锂和其他烷基铵盐。 对于冷冻,尝试将盐浓度提高 20%。 (冷冻盐:大分子晶体学中冰形成的抑制,K. A. Rubinson 等人,Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587。

更换CryoLoop环的方法与建议

更换CryoLoop环的方法与建议

Replacing a Mounted CryoLoop in a CrystalCap 更换 CrystalCap 中已安装的 CryoLoop

Removal 
With one hand, using pliers, grasp the Mounted CryoLoop. Using your the other hand, hold the CrystalCap between your thumb and finger(s). With gentle authority twist the Mounted CryoLoop and Cap in opposing directions and pull the Mounted CryoLoop from the CrystalCap.

Replacement 
Select a Mounted CryoLoop with the desired CryoLoop size. Notice the notches on the Mounted CryoLoop. A standard length Mounted CryoLoop is created by snapping the MicroTube at the second notch from the bottom. Remove the end using your fingers or diagonal cutter. Apply a drop of Super Glue to the top opening of the CrystalCap. Insert the Mounted CryoLoop into this opening and push until the Mounted CryoLoop is stopped by the bottom of the CrystalCap. Epoxy may be used instead of Super Glue for a more permanent fixation of the Mounted CryoLoop to the CrystalCap.

移动
用一只手用钳子抓住已安装的 CryoLoop。 用另一只手将 CrystalCap 夹在拇指和其他手指之间。 以温和的权威将已安装的 CryoLoop 和 Cap 朝相反方向扭转,然后将已安装的 CryoLoop 从 CrystalCap 中拉出。

替代品
选择具有所需 CryoLoop 尺寸的已安装 CryoLoop。 注意已安装的 CryoLoop 上的槽口。 标准长度的已安装 CryoLoop 是通过在从底部开始的第二个凹口处卡入 MicroTube 来创建的。 用手指或斜切刀去除末端。 在 CrystalCap 的顶部开口处涂抹一滴强力胶。 将已安装的 CryoLoop 插入此开口并推动,直到已安装的 CryoLoop 被 CrystalCap 的底部挡住。 可以使用环氧树脂代替强力胶,以便将已安装的 CryoLoop 更永久地固定在 CrystalCap 上。

蛋白晶体出现冰环解决方案

蛋白晶体出现冰环解决方案

Ice Rings 冰环

Got ice rings and want to get rid of them?

While the crystal is in the beam, carefully try the following.

Try annealing the crystal by blocking the stream for a few seconds, allowing the crystal to thaw, then unblock the stream to cool the crystal again. (Reference: New techniques in macromolecular cryocrystallography: macromolecular crystal annealing and cryogenic helium, B. Leif Hansona, Constance A. Schallb and Gerard J. Bunick, Journal of Structural Biology
Volume 142, Issue 1, April 2003, Pages 77-87)

Try washing the crystal with liquid nitrogen. Using a pipette, carefully aspirate liquid nitrogen into the pipette, then dispense several drops or even a gentle stream over the crystal and mount. A liquid nitrogen wash can also be used to remove creeping ice from caps, pins and loops.

有冰环并想摆脱它们?

当晶体在光束中时,请仔细尝试以下操作。

尝试通过阻塞流几秒钟来使晶体退火,让晶体解冻,然后解除阻塞流以再次冷却晶体。 (参考:高分子低温晶体学新技术:高分子晶体退火和低温氦,B. Leif Hansona,Constance A. Schallb 和 Gerard J. Bunick,Journal of Structural Biology
第 142 卷,第 1 期,2003 年 4 月,第 77-87 页)

尝试用液氮清洗晶体。 使用移液器小心地将液氮吸入移液器中,然后在晶体上滴几滴甚至是轻柔的液流并安装。 液氮清洗也可用于去除盖子、销钉和环上的爬行冰。

在普通结晶试剂添加硫酸铵和盐

Cryo for Ammonium sulfate and salt based crystallization reagents   用于硫酸铵和盐基结晶试剂的 Cryo

Increase the concentration of ammonium sulfate (cryosalt) (or primary salt acting as a precipitant) in increments of 10% in the presence of 5 to 10% v/v Glycerol.

Evaluate 25-30% w/v glucose, or trehalose or sucrose as cryoprotectants for crystals grown in Ammonium sulfate. Acta Cryst. (2002). D58, 1664-1669. Crystallization of RNA/protein complexes. M. Garber, G. Gongadze, V. Meshcheryakov, O. Nikonov, A. Nikulin, A. Perederina, W. Piendl, A. Serganov and S. Tishchenko.

15 to 30% v/v Ethylene glycol, DMSO, or Glycerol.

Try 1 – 2 M Sodium malonate as a cryoprotectant. Acta Cryst. (2003). D59, 2356-2358. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko.

Place a drop of 75% v/v Paratone-N, 25% v/v Paraffin Oil next to the drop containing the crystal. Remove the crystal from the drop using a CryoLoop and dip the crystal into the oil. Keeping the CryoLoop and crystal immersed, gently move the CryoLoop containing the crystal from the oil into the reagent, back into the oil, back into the reagent, repeating this several times to remove some of the aqueous (reagent) layer from the crystal surface. Cryo cool the crystal.

在 5 至 10% v/v 甘油存在的情况下,以 10% 的增量增加硫酸铵(冰晶盐)(或用作沉淀剂的初级盐)的浓度。

评估 25-30% w/v 葡萄糖、海藻糖或蔗糖作为在硫酸铵中生长的晶体的冷冻保护剂。晶体学报。 (2002 年)。 D58,1664-1669。 RNA/蛋白质复合物的结晶。 M. Garber、G. Gongadze、V. Meshcheryakov、O. Nikonov、A. Nikulin、A. Perederina、W. Piendl、A. Serganov 和 S. Tishchenko。

15 至 30% v/v 乙二醇、DMSO 或甘油。

尝试 1 – 2 M 丙二酸钠作为冷冻保护剂。晶体学报。 (2003 年)。 D59,2356-2358。丙二酸盐:一种用于盐生大分子晶体的多功能冷冻保护剂和稳定溶液。 T. Holyoak、T. D. Fenn、M. A. Wilson、A. G. Moulin、D. Ringe 和 G. A. Petsko。

在含有晶体的液滴旁边放置一滴 75% v/v Paratone-N、25% v/v 石蜡油。使用 CryoLoop 从液滴中取出晶体并将晶体浸入油中。保持 CryoLoop 和晶体浸没,轻轻地将含有晶体的 CryoLoop 从油中移入试剂中,再移回油中,再移回试剂中,重复几次以去除晶体表面的一些水(试剂)层。低温冷却晶体。

Cleaning a CryoLoop   CryoLoop环的清洗方法

Cleaning a CryoLoop   CryoLoop环的清洗方法

Soak the CryoLoop in a 0.5% detergent solution in deionized water for 15 minutes. Gently rinse the CryoLoop in deionized water. Allow the CryoLoop to air dry.

If more rigorous cleaning is desired or one does not wish to risk the presence of trace amounts of residual detergent, soak the CryoLoop in 5.0 M Urea formulated in deionized water for 15 minutes. Gently rinse the CryoLoop in deionized water. Repeat three times. Allow the CryoLoop to air dry.

将 CryoLoop 浸泡在 0.5% 去离子水中的清洁剂溶液中 15 分钟。 在去离子水中轻轻冲洗 CryoLoop。 让 CryoLoop 风干。

如果需要更严格的清洁或不想冒残留洗涤剂残留的风险,请将 CryoLoop 浸泡在 5.0 M 去离子水中配制的尿素中 15 分钟。 在去离子水中轻轻冲洗 CryoLoop。 重复三遍。 让 CryoLoop 风干。