Hampton 蛋白质结晶试剂和耗材介绍

特色

Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域受信赖的品牌之一。

上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Hampton research主要产品:

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop

Crystallization Reagents

定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商之一,该公司的产品目录是由上海金畔生物科技有限公司提供,该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类:Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test(PCT预结晶试验)

应用:结晶筛选,确定相应的样品浓度,PCT试验用来预结晶结晶筛选,以确定适当的蛋白质浓度

  1. Slice pH(PH切片)

应用:溶解度和结晶筛选

  1. Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

  1. Index

应用:不同的试剂系统的结晶屏幕的蛋白质复合物,多肽,核酸,水溶性小分子。

  1. Crystal Screen(晶体屏幕)

应用:

主屏幕的可溶性肽,蛋白质,核酸,和水溶性小分子。稀疏矩阵的加法屏幕

5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质,复合物,缩氨酸,核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质,缩氨酸,核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质,缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质,缩氨酸,核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶,分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂,与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体,单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化

16.Natrix?用于核酸,蛋白核酸聚合物的结晶

17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂,用于区分蛋白晶体和盐晶体

第二类:Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类:Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体

2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类

5.Optimize-Buffers缓冲液

6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物

8.Optimize-Cryoprotectants

9.Optimize-Oils用于结晶的油类

10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类:StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类:Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶

8.Micro-Bridges?微桥-坐滴结晶的支撑物

9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂,油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类:Tools, Seeding & Resin(结晶工具、树脂)

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool

第七类:Cryocrystallography

  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类:Capillary Mounts & Supplies(毛细管座椅支架供应)

第九类:Goniometer Heads & Supplies(测角头及用品)

第十类:Xenon Derivatization(氙气衍生物)

第十一类:Labels & Pens(标签和记录笔)

第十二类:Protein Crystallization Standards(蛋白质结晶的标准)

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

Hampton代理,欢迎访问Hampton官网或者咨询我们获取更多相关产品信息。

上海金畔生物科技有限公司(www.jinpanbio.cn)提供生命科学研究领域系列产品,包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;WAKO日本和光纯药、修饰性PEG(Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、小鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

Hampton蛋白结晶工具及耗材

特色

Hampton蛋白结晶工具及耗材

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™ – 20 micron

HR4-974 18 mm Mounted CryoLoop 0.7 - 1.0 mm

HR4-974

Mounted CryoLoop™ – 20 micron

APPLICATIONS

  • Cryocrystallography

HR4-965 Mounted CryoLoop 0.7 - 1.0 mm

HR4-965

Hampton 蛋白结晶loop环 Mounted CryoLoop 10 micron with EasySnap MicroTube

HR4-994 Mounted CryoLoop 0.025 - 0.05 mm

CAT NO NAME DESCRIPTION
HR4-994 Mounted CryoLoop – 10 micron 0.025-0.05 mm – 25 pack
HR4-995 Mounted CryoLoop – 10 micron 0.05 – 0.1 mm – 25 pack
HR4-997 Mounted CryoLoop – 10 micron 0.1 – 0.2 mm – 25 pack
HR4-999 Mounted CryoLoop – 10 micron 0.2 – 0.3 mm – 25 pack

Adjustable Mounted CryoLoop™ with EasySnap MicroTube

HR8-110 Adjustable Mounted CryoLoop 0.7 - 1.0 mm

Applications
Cryocrystallography
Features
Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

Description 
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.

Using forceps or a probe one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.

CAT NO NAME DESCRIPTION
HR4-338 Adjustable Mounted CryoLoop 0.05 – 0.1 mm – 25 pack
HR5-900 Adjustable Mounted CryoLoop Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)
HR4-336 Adjustable Mounted CryoLoop 0.025 – 0.05 mm – 25 pack
HR8-102 Adjustable Mounted CryoLoop 0.1 – 0.2 mm – 25 pack
HR8-072 Adjustable Mounted CryoLoop 0.2 – 0.3 mm – 25 pack
HR8-104 Adjustable Mounted CryoLoop 0.3 – 0.4 mm – 25 pack
HR8-106 Adjustable Mounted CryoLoop 0.4 – 0.5 mm – 25 pack
HR8-108 Adjustable Mounted CryoLoop 0.5 – 0.7 mm – 25 pack
HR8-110 Adjustable Mounted CryoLoop 0.7 – 1.0 mm – 25 pack

Hampton蛋白结晶小瓶和结晶帽CrystalCap™

CrystalCap™ ALS

Applications
Cryocrystallography
Features
ALS style crystal mount for cryocrystallography
CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
Description 
The CrystalCap ALS is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

CrystalCap ALS (HR4-779) is supplied without a vial (HR4-904).

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS is compatible with ALS style grippers, auto mounters and sample handlers.

ALS style sample mounters accept the CrystalCap ALS.

HR4-779    CrystalCap ALS     without Vial – 60 pack

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers

CrystalCap

APPLICATIONS

  • Cryocrystallography

FEATURES

  • SSRL style sample mount for cryocrystallography
  • CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers
CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

CrystalCap & Vial

Hampton CrystalCap™ SPINE HT

Applications
Cryocrystallography

Features  
SPINE style sample mount for cryocrystallography
CrystalCap SPINE HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base

Hollowed out design compatible with SPINE style grippers, auto mounters and sample handlers

2D alphanumeric coding for sample tracking & management
Bar coded, color coded, alphanumeric, magnetic cap
Color coded CryoLoop size
Description
The CrystalCap SPINE HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SPINE style caps. The CrystalCap SPINE HT attaches to a magnetic CrystalCap SPINE Vial and magnetic goniometer base. The tip of the CrystalCap SPINE HT accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap SPINE Vial is a 1.8 ml cryo vial featuring two small vents and is compatible with the CrystalCap SPINE HT. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The CrystalCap SPINE Vial features chamfered edges for enhanced cap positioning as well as a magnetic alloy bottom for stability. The HR8-114 CrystalCap SPINE Vial does have a magnet on the bottom of the vial.

The hollowed out design of the CrystalCap SPINE HT is compatible with SPINE style grippers, auto mounters and sample handlers.

The CrystalCap SPINE HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CAT NO NAME DESCRIPTION
HR8-118 CrystalCap SPINE HT 0.025-0.05 mm CryoLoop, without Vial – 30 pack
HR8-120 CrystalCap SPINE HT 0.05-0.1 mm CryoLoop, without Vial – 30 pack
HR8-122 CrystalCap SPINE HT 0.1-0.2 mm CryoLoop, without Vial – 30 pack
HR8-124 CrystalCap SPINE HT 0.2-0.3 mm CryoLoop, without Vial – 30 pack
HR8-126 CrystalCap SPINE HT 0.3-0.4 mm CryoLoop, without Vial – 30 pack
HR8-128 CrystalCap SPINE HT 0.4-0.5 mm CryoLoop, without Vial – 30 pack
HR8-130 CrystalCap SPINE HT 0.5-0.7mm CryoLoop, without Vial – 30 pack
HR8-132 CrystalCap SPINE HT 0.7-1.0 mm CryoLoop, without Vial – 30 pack
HR8-112 CrystalCap SPINE HT Cap only – 30 pack
HR8-116 CrystalCap SPINE HT Cap/Vial Cap/Vial only – 30 pack
HR8-094 CrystalCap SPINE without 2D code/printing Cap only – 30 pack
HR8-114 CrystalCap SPINE Vial Vial only – 30 pack

CrystalCap™ ALS HT

Applications

Cryocrystallography

Features
ALS style crystal mount for cryocrystallography
CrystalCap ALS HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
2D alphanumeric coding for sample tracking & management
Color coded CryoLoop size
Description
The CrystalCap ALS HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS HT attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS HT is compatible with ALS style grippers, auto mounters and sample handlers.

The CrystalCap ALS HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CAT NO NAME DESCRIPTION
HR8-200 CrystalCap ALS HT 0.025-0.05 mm CryoLoop – 30 pack
HR8-202 CrystalCap ALS HT 0.05-0.1 mm CryoLoop – 30 pack
HR8-204 CrystalCap ALS HT 0.1-0.2 mm CryoLoop – 30 pack
HR8-206 CrystalCap ALS HT 0.2-0.3 mm CryoLoop – 30 pack
HR8-208 CrystalCap ALS HT 0.3-0.4 mm CryoLoop – 30 pack

Hampton 石英毛细管 Quartz Capillaries

Applications

X-ray data collection
Liquid-liquid diffusion crystallization
Gel acupuncture crystallization

Features
Quartz
Thin walled – 10 micron

CAT NO NAME DESCRIPTION
HR6-128 Quartz Capillary Size: 0.1 mm – 25 pack
HR6-130 Quartz Capillary Size: 0.2 mm – 25 pack
HR6-132 Quartz Capillary Size: 0.3 mm – 25 pack
HR6-134 Quartz Capillary Size: 0.4 mm – 25 pack
HR6-136 Quartz Capillary Size: 0.5 mm – 25 pack
HR6-138 Quartz Capillary Size: 0.6 mm – 25 pack
HR6-140 Quartz Capillary Size: 0.7 mm – 25 pack
HR6-142 Quartz Capillary Size: 0.8 mm – 25 pack
HR6-144 Quartz Capillary Size: 0.9 mm – 25 pack
HR6-146 Quartz Capillary Size: 1.0 mm – 25 pack
HR6-148 Quartz Capillary Size: 1.5 mm – 25 pack
HR6-150 Quartz Capillary Size: 2.0 mm – 25 pack
HR6-151 Quartz Capillary Size: 2.5 mm – 15 pack
HR6-175 Quartz Capillary Size: 3.0 mm – 15 pack
HR6-177 Quartz Capillary Size: 4.0 mm – 5 pack
HR6-179 Quartz Capillary Size: 5.0 mm – 5 pack
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Spearlab Dewars
HR4-662 Cryogenic Foam Dewar (500 ml) – Small  500ml规格
HR4-675 – Spearlab Cryogenic Foam Dewar (2 liter)  2L规格

Applications 应用

Cryocrystallography 蛋白结晶学

Hampton 结晶板封口膜ClearSeal Film™ and Applicator

Applications 应用

Sealing film used to seal sitting drop crystallization experiments  使用密封膜来密封座滴结晶实验

Features  特征
1.High optical clarity, low auto-fluorescence
2.Easy to handle film; easy to remove liner
3.Pressure sensitive adhesive
4.For best results use specially designed applicator
5.UV transmissable, compatible with UV imaging systems
6.Low birefringence
7.Certified DNase-, RNase-, and nucleic-acid-free
8.Silicone adhesive is non-tacky until pressed against the sealing surface with the applicator
9.Chemically inert; no extractable except at extreme pH
10.Excellent chemical resistance (e.g. DMSO, Methanol, Acetonitrile)
11.Heat and cold resistant, recommended for temperatures from -70°C and 110°C

特征
高光学透明度、低自体荧光
膜易于处理 ;易拆卸衬垫
压力敏感粘合剂
为了获得佳效果 , 请使用专门设计的涂抹器
传染性紫外、紫外成像系统兼容
低双折射
认证 DNase – , RNase – , 和核酸的游离
硅酮粘合剂是无粘性的 , 直到压在密封表面与涂抹器
化学惰性的 ;除了极端 pH 不能提取优异的耐化学性 (如。DMSO 、甲醇、乙腈)
耐热耐寒 , 温度从 – 70 ° C 至 110 ° C

ClearSeal Film Before and After

Description
ClearSeal Film fits all SBS format 96 well plates. Minimal evaporation, minimal cross-well contamination, easy to handle, and optically clear. Single coated, 2 mil (0.05 mm), optically clear polyolefin film with pressure-sensitive silicone-based adhesive. Supplied as a 141 mm x 78 mm film with white polyester release liner (backing). Actual seal area after end tabs removed is 120 x 78 mm Seals well to polypropylene, polystyrene, and polycarbonate plates. Compatible with aqueous and organic solvents. Suitable for use between -70 and 110°C. For best seal, the plate must be sealed with the Film Sealing Paddle HR4-513. ClearSeal Film has a limited shelf life. Must be used before expiration date printed on box. Each pack of film (HR4-521) includes one free Film Sealing Paddle (available separately as HR4-413).

HR4-413 Film Sealing Paddle, 3 7/8″ x 2 15/16″, polyethylene, white. 5 paddles per pack. An alternative to the plate roller or brayer for pressing films to assure a secure uniform seal around all wells on 96-well, 384-well, and 1536-well plates. These paddles are especially recommended for sealing films on raised-rim plates because they fit within the rim of the plate.

Store ClearSeal Filmm at 20°C +/- 10 degrees at 50% RH +/- 10%.

Hampton 石蜡和硅润滑油Paraffin & Silicon Oil

Applications  应用
Microbatch crystallization    microbatch 结晶
Features  特征
Under oil crystallization 在油中结晶
Protect the sample from oxidation  保护样品免于氧化
Screen different temperatures without condensation   屏幕不同温度下不凝结
Description   描述 
Oils used for microbatch and modified microbatch crystallization under oil.

使用的油和改性 microbatch microbatch 结晶油。

Al’s Oil is a 50:50 (volume:volume) mixture of Paraffin Oil and Silicon Oil. Al’s Oil is named after it’s inventor, Allan D’Arcy.

Al 是机油的 50 ∶ 50 (体积 ∶ 体积) 混合物的石蜡油和硅油。Al 的石油是其发明者的名字命名 , Allan D ‘Arcy) 。

Silicon Oil

Paraffin oil
Synonym: Mineral oil
CAS Number [8042-47-5]
EC Number 232-455-8
RTECS PY8047000
MDL Number MFCD00131611
Appearance Colorless, clear, viscous liquid
Refractive Index n20/D 1.467 (literature) n20/D 1.468
IR Spectroscopy Grade
Density 0.838 g/mL at 25°C (literature) 0.85 g/ml at 20°C
Viscosity 40-42 CST at 25°C

Silicon Oil 
Synonyms: Octamethyltrisiloxane
CAS Number: 107-51-7
Appearance: Colorless, clear viscous liquid
Melting Point: -86°C
Boiling Point: 152°C
Refractive Index: 1.38 at 25°C
Viscosity: 1 CST at 25°C
Specific Gravity: 0.82 at 25°C
Density: 0.82 g/mL at 25°C
Surface Tension: 17.4 mN/m

CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
HR4-670 Vial Clamp - Straight
HR4-670
CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, Straight HR4-315 CrystalWand Magnetic, 45° (McMiken Tool)

图片

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Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°图片

CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool
Cap Container (empty)
HR8-016 Cap Container Opened
HR8-016

Applications

Holds 30 CrystalCap caps for storage or transport
Features
Black polypropylene box with overlap snap closure
Holds 30 caps with or without Mounted CryoLoops™
Description 
Empty black plastic box designed to hold 30 CrystalCap Copper Magnetic HT, CrystalCap Copper Magnetic ALS HT, CrystalCap HT (SPINE), CrystalCap Magnetic or CrystalCap Copper Magnetic without vials. Useful for shipping caps that do not need to be frozen or returning used caps home after a synchrotron trip. The Cap Container is designed for use with Caps only and does not accommodate vials.

Hampton 水晶透明密封带Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Hampton Tungsten Carbide Scribe & Glass Cutter with Replaceable Tips

Hampton Tungsten Carbide Scribe & Glass Cutter with Replaceable Tips

碳化钨刀头&可更换刀尖的玻璃切割机

APPLICATIONS

  • Useful for scribing, cutting and removal of the upper coverglass that seals the LCP Sandwich Set.

FEATURES

  • Machined, lightweight aluminum handle
  • Replaceable tungsten carbide scribe tip
  • Diamond knurling for comfortable and sure grip
  • Pocket clip
  • Size: 8 mm diameter, 130 mm length
  • Reversible tip protects tip and user

DESCRIPTION

Tungsten Carbide scribe and glass cutter with replaceable tungsten carbide tip. Tungsten Carbide Tip can be removed and inverted to protect tip and user. Knurled aluminum alloy body and pocket clip. Overall length 130 mm. Useful for cutting and removal of the upper coverglass that seals the HR3-151 LCP Sandwich Set.

Tungsten carbide scribe tips are not threaded and are held in place with a threaded tip chuck. To remove tip, turn the tip chuck counterclockwise one turn (without completely removing the chuck) while holding the aluminum handle stationary, and pull the tip from the chuck and handle. To install a new tip, slide the new tip into the chuck and handle and tighten the tip chuck clockwise to secure the new tip into the handle. Loosen tip chuck, remove tip, reserve and install with the tip inverted to protect user and the tip when not in use.

Tungsten Carbide Scribe is supplied with one tip installed. Replacement tips sold separately. Tungsten Carbide Scribe Tip is a set of 3 replacement tips designed for the Tungsten Carbide Scribe HR4-702. Tungsten carbide Tip in steel shank to strengthen and protect carbide from damage. Scribe Tip Diameter x Length: 3 mm X 32 mm.

See the JOVE video for more details about using the Tungsten Carbide glass cutter to open LCP Sandwich Set experiments:

HR4-702 Tungsten Carbide Scribe Each
HR4-703 Tungsten Carbide Scribe Tip Pack of 3 tips

应用

  • 适用于划线,切割和拆卸上盖板,密封LCP三明治套。

特征

  • 机加工的,轻铝手柄
  • 可更换碳化钨针尖
  • 钻石滚花舒适可靠的抓地力
  • 袖珍夹
  • 尺寸:直径8毫米,长度130毫米
  • 可逆提示保护提示和用户

描述

碳化钨切割机和可更换碳化钨针尖的玻璃切割机。碳化钨针尖可以拆卸和倒置,以保护针尖和使用者。滚花铝合金阀体和口袋夹。总长130毫米。用于切割和移除上盖板,密封HR3-151 LCP夹层套。

碳化钨刀尖不是螺纹的,而是用螺纹夹头固定在适当的位置。若要移除夹头,请在保持铝制手柄固定的同时逆时针旋转一次(不完全移除卡盘),并从卡盘和手柄上拉出夹头。若要安装新的尖端,请将新的尖端滑动到卡盘中,并按顺时针方向拧紧夹头卡盘,以将新的尖端固定到手柄中。松开刀尖卡盘,拆下针尖,保留并安装倒装,以保护使用者和未使用时的针尖。

碳化钨Scribe是提供与一个尖端安装。更换提示单独出售。碳化钨刀尖是为碳化钨Scribe HR4-702设计的一套3种替代尖端。钢柄碳化钨针尖强化和防止碳化物损坏。划线头直径x长度:3毫米x 32毫米。

RELATED ITEM(S)

REFERENCES

1. Li, D., Boland, C., Walsh, K., Caffrey, M. Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases. J. Vis. Exp. (67), e4000, DOI: 10.3791/4000 (2012).

2. Cherezov, V., and M. Caffrey. (2003) Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. J. Appl. Cryst. 36: 1372-1377.

3. Cherezov, V., Peddi, A., Muthusubramaniam, L., Zheng, Y.F., and M. Caffrey. (2004) A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr. D Biol. Crystallogr. 60: 1795-1807.

4. Caffrey, M., Li, D., & Dukkipati, A. Membrane protein structure determination using crystallography and lipidic mesophases – recent advances and successes. Biochemistry. DOI: 10.1021/bj300010w, 2012.

Hampton Capillary Cutting Stone

Hampton Capillary Cutting Stone

APPLICATIONS

  • The easiest way to cut capillaries

FEATURES

  • Score and snap.
  • Ceramic cutting stone

DESCRIPTION

These small (25 mm x 25 mm x 0.65 mm) cutting stones are used to make clean cuts of glass and quartz capillaries. Use the edge of the stone to etch the capillary then gently snap the capillary in two. This is useful for cutting the tips off closed capillaries used for mounting single crystals for x-ray diffraction analysis or for cutting capillaries to the desired length.

Capillary Cutting Procedure

1. Gently place the capillary on a clean, flat surface.
2. Gently holding the stone at approximately 30° angle to the capillary tube, starting near one edge of the stone, proceeding in a single, smooth motion, draw the length of the non-serrated edge of the stone across the capillary one time . Apply just enough pressure to score the capillary, do not attempt to cut through the capillary.
3. Pull the capillary axially until it breaks. If it won’t break, the score was not deep enough. Repeat the above steps, pressing down with slightly more force while drawing the cleaving stone the capillary.
4. Once cut, inspect the end finish to ensure the cut quality meets the application requirements.

毛细管切割石材™

应用

  • 切毛细血管最简单的方法

特征

  • 分分秒秒。
  • 陶瓷切割石

描述

这些小切割石(25毫米x25毫米x 0.65毫米)被用来切割玻璃和石英毛细血管。用石头的边缘蚀刻毛细,然后轻轻地把毛细拧成两半。这是有用的切尖关闭关闭毛细管用于安装单晶x射线衍射分析或切割毛细血管到所需的长度。

毛细管切割程序

1.轻轻地将毛细放在干净、平坦的表面上。
2.以大约30°的角度轻轻握住毛细管,从石的边缘开始,以一次平滑的方式进行,一次画出石的非锯齿状边缘的长度。施加足够的压力来打分毛细血管,不要试图切断毛细血管。
3.轴向拉毛,直至毛细血管断裂。如果它不能打破,比分是不够深的。重复上面的步骤,用稍微大一点的力量按压下来,同时把切割的石头拉到毛细上。
4.切割后,检查成品,确保切割质量符合使用要求。

RELATED IT

HR4-334 Capillary Cutting Stone each、

EM(S)

Hampton LCP Sandwich Set

Hampton LCP Sandwich Set

LCP夹层集

应用

  • Lipidic中间相膜蛋白的结晶筛选及二段法和分批法

特征

  • 小体积LCP、双胶束和微间歇结晶
  • 高质量玻璃光学
  • 由于中间相团被物理地夹在两个光学清晰的表面之间,消除了中间相/水介质界面和相应的粗糙度,因此具有优良的滴光光学性能。
  • 玻璃板允许使用交叉偏振片之间的无双折射检查来显示微晶。
  • 超疏水玻璃表面
  • 光场、紫外光和荧光显微镜的优化
  • 稳定性好,耐蒸发

描述

LCP夹芯片组由一个底座玻璃滑块和一个优化的盖板滑块组成。LCP三明治集与著名的斯克里普斯研究所共同开发,位于美国加利福尼亚州拉霍拉,由保罗马里恩菲尔德(0890003)制造。

LCP玻璃夹芯片组是一种专门设计的平板,可用于96 Lipidic立方相矩阵筛选实验的自动或手动设置。本发明还可用于二段法和分批法。薄片(<2mm高)具有优良的光学性能,非常适合于微晶体的探测和交叉偏振片之间的无双折射成像。该平板允许在中间结晶试验中,50纳米色素,蛋白质/脂质中间相和1微升沉淀剂溶液每次试验。

96井LCP玻璃夹层板由一个127.8×85.5x1mm玻璃基板和一个带有SBS兼容板足迹的140米厚的双粘性间隔板和一个0.2毫米厚的玻璃盖板组成。双粘垫片已粘在基板上。牛皮纸衬垫覆盖和保护双粘性间隔和基板顶部。0.2毫米厚的玻璃盖板适合和密封整个96井下板。另外,一系列的24硅化18x18mm正方形盖板(HR3-152)可以用来密封整个96井下板。每个18×18毫米1盖滑动密封4井。条形码的空间可用在板的左端。每口井可含立方相纳米粒子50粒,结晶试剂1微升。

18x18mm 1号硅化盖板由第一级水解级的抗化学腐蚀硼硅酸盐玻璃D 263 M制成。该幻灯片无色,清晰,适用于荧光显微镜。幻灯片的两侧都有一个超疏水的表面。幻灯片是没有的。厚度(0.13ro 0.16 mm)。幻灯片以两个隔间塑料箱提供,每箱100张幻灯片,每箱10盒,HR3-152型1,000张幻灯片。

碳化钨玻璃切割机可用于切割和拆卸密封LCP夹层套的上盖板。单独出售。

APPLICATIONS

  • Crystallization screening of membrane proteins in lipidic mesophase as well as bicelle method and batch method

FEATURES

  • Small volume LCP, bicelle and microbatch crystallization
  • High quality glass optics
  • Excellent drop optics since mesophase bolus is physically sandwiched between two optically clear surfaces eliminating the mesophase / aqueous medium interface and the corresponding roughness
  • Glass plate allows for visualization of microcrystals using birefringence-free examination between crossed polarizers
  • Superhydrophobic glass surfaces
  • Optimized for bright field, UV and fluorescent microscopy
  • Excellent stability, resistant to evaporation

DESCRIPTION

The LCP Sandwich Set consists of a base glass slide and an optimized cover slip. LCP Sandwich Set developed jointly with the renowned Scripps Research Institute in La Jolla, California, USA and is manufactured by Paul Marienfeld (0890003).

The LCP Glass Sandwich Set is a specially designed plate for either automated or manual setting of 96 Lipidic Cubic Phase matrix screening experiments. The plate can also be used for the bicelle method and batch method. The thin (< 2 mm high) plates have exquisite optical properties and are well suited for the detection of microcrystals and for birefringence-free imaging between crossed polarizers. The plates allow for in meso crystallization trials with 50 nanoliters protein/lipid mesophase and 1 microliter precipitant solution per trial.

The 96 well LCP Glass Sandwich Plate consists of a) 127.8 x 85.5 x 1 mm glass base plate with the footprint of an SBS-compliant plate, a 140 µm thick double sticky spacer with 96 punched out holes and b) a 0.2 mm thick glass coverslip. The double sticky spacer is already adhered to the base plate. A brown paper liner covers and protects the top of the double sticky spacer and base plate. The 0.2 mm thick glass coverslip fits over and seals the entire 96 well lower plate. Alternatively a series of twenty-four siliconized 18 x 18 mm No. 1 square cover slides (HR3-152) can be used to seal the entire 96 well lower plate. Each 18 x 18 mm No. 1 cover slide seals 4 wells. Space for a bar code is available at the left end of the plate. Each well can contain 50 nanoliters of cubic phase and 1 microliter of crystallization reagent.

18 x 18 mm No. 1 Siliconized Cover Slides are made from chemically resistant borosilicate glass D 263 M of the first hydrolytic class. The slides are colorless, clear, and suitable for fluorescence microscopy. The slides feature a super hydrophobic surface on both sides. The slides are No. thickness (0.13 ro 0.16 mm). The slides are supplied in a two compartment plastic box, 100 slides per box, 10 boxes per case, 1,000 slides total for HR3-152.

The Tungsten-carbide glass cutter can be used for cutting and removal of the upper coverglass that seals the LCP Sandwich Set. Sold separately.

HR3-151 LCP Sandwich Set Pack of 20
HR3-152 18 x 18 mm No. 1 Siliconized Cover Slides, Squares Case of 10 packs、

ELATED ITEM(S)

REFERENCES

1. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. Vadim Cherezov and Martin Caffrey. J. Appl. Cryst. (2003). 36, 1372-1377.

2. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Vadim Cherezov, Avinash Peddi, Lalitha Muthusubramaniam, Yuan F. Zheng and Martin Caffrey. Acta Cryst. (2004). D60, 1795-1807 DOI: 10.1107/S0907444904019109.

3. Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. Faham, S. & Bowie, J. U. (2002). J. Mol. Biol. 316, 1-6.

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Hampton蛋白质结晶捞针(结晶环)18 mm Mounted CryoLoop™

Applications :
Cryocrystallography

应用

  • 冰晶术

Features:
Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

特征

  • 准备使用18毫米长度的冷冻环微管
  • 不锈钢微管上的合成冷冻环
  • 环形直径范围为0.025~1.0mm
  • 兼容水晶帽、冰帽脊骨

Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.

Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.

The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.

18 mm Mounted CryoLoop

18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic

Crystal in CryoLoop

描述

18毫米装有20微米直径尼龙的冷冻鞋。尼龙环是钉在空心的不锈钢微管™,用于安装,冻结,并确保晶体在冰晶过程和X射线数据收集。微管上的18毫米安装超低温是18毫米的长度,并准备插入到水晶帽,磁性和水晶帽HT。该20微米直径尼龙环显示最小的衍射,是薄的快速冷冷却晶体,强大,空气动力学。

适用于水晶帽、水晶帽和水晶帽脊柱的HT系统。不兼容水晶帽铜,水晶帽铜HT,或水晶帽ALS HT。

CAT NO NAME DESCRIPTION
HR4-932 18 mm Mounted CryoLoop – 20 micron 0.025 – 0.05 mm – 25 pack
HR4-945 18 mm Mounted CryoLoop – 20 micron 0.05 – 0.1 mm – 25 pack
HR4-947 18 mm Mounted CryoLoop – 20 micron 0.1 – 0.2 mm – 25 pack
HR4-970 18 mm Mounted CryoLoop – 20 micron 0.2 – 0.3 mm – 25 pack
HR4-971 18 mm Mounted CryoLoop – 20 micron 0.3 – 0.4 mm – 25 pack
HR4-972 18 mm Mounted CryoLoop – 20 micron 0.4 – 0.5 mm – 25 pack
HR4-973 18 mm Mounted CryoLoop – 20 micron 0.5 – 0.7 mm – 25 pack
HR4-974 18 mm Mounted CryoLoop – 20 micron 0.7 – 1.0 mm – 25 pack
HR4-918 18 mm Mounted CryoLoop – 20 micron Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

 4 Related Items  相关产品
CrystalCap™
CrystalCap™ ALS
CrystalCap™ SPINE HT
Epoxy

Hampton Mounted CryoLoop – 20 micron with EasySnap MicroTube

Hampton 蛋白结晶loop环 Mounted CryoLoop 10 micron with EasySnap MicroTube

Hampton蛋白结晶柱 CrystalCap™ SPINE HT

Hampton蛋白结晶小瓶和结晶帽CrystalCap™

Hampton HR3-251 PEN-VAC Kit 使用说明

Hampton HR3-251 PEN-VAC Kit 使用说明

APPLICATIONS

  • Holding and manipulating small, flat surfaces such as glass cover slides

FEATURES

  • Self-contained vacuum
  • No power needed
  • Compact

DESCRIPTION

PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.

HR3-251 PEN-VAC Kit each

笔-VAC试剂盒

应用

  • 保持和操纵小的、平坦的表面,如玻璃盖板滑梯

特征

  • 自给真空
  • 不需要电力
  • 紧凑型

描述

笔-VAC是一个伟大的工具,处理小的,平面表面的物体,如平原和硅化覆盖幻灯片,以及塑料幻灯片。只需按下侧面按钮,按一下要提起的物体的尖端,然后松开按钮。笔-VAC创造了一个完全独立的真空,提升高达50克,长达一分钟。不到一盎司,轻巧的铝制机身就可以装在口袋里了。不需要电源。该试剂盒包括一个5 3/4“蓝色车身的笔-VAC,(3)角度和(3)直探头,每一个1/8”,1/4“,3/8”直径的蓝色硅真空杯,它被包装在一个清晰的塑料储存箱中。

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Hampton HR3-105 MASTERBLOCK® 96 Deep Well (Greiner)

Hampton HR3-105 MASTERBLOCK® 96 Deep Well (Greiner)

APPLICATIONS

  • 96 Deep Well block for crystallization screen reagents

FEATURES

  • SBS format, 96 well
  • Sterile, individually sealed plates
  • Polypropylene
  • Seal with AlumaSeal II, Crystal Clear Sealing Film, or Cap Mats

DESCRIPTION

The Greiner 780261 MASTERBLOCK® is a 96 Deep Well polypropylene block (plate). Each well has a fill volume of 1.2 ml. The MASTERBLOCK® is useful for holding crystallization screen reagents when using liquid handling automation or multi channel pipets. Each plate is sterile and packaged individually. Round bottom well for complete reagent recovery. ClearChoice™ virgin polypropylene resin (autoclavable to 121°C). SBS recommended dimensions. Alphanumerically coded wells with chimney-style, round well top.

The Microplate Lid is a temporary cover for the crystallization screens in 96 deep well format. The single position, high profile cover with individual condensation rings over each reagent well can help minimize reagent evaporation from 96 deep well reagent plates as well as protect the reagents from particulate and contamination. The individually wrapped, sterile Microplate Lid is made from optically clear polystyrene. Greiner 656171.

The Cap Mat is made of ethyl vinyl acetate (EVA). Resistant to DMSO and can be used in a temperature range between -20 and +60 degrees Celsius. Suitable for sealing Greiner 780261 1 milliliter Deep Well Plate (HR3-105). These cap mats are not piercable. Supplied sterile. Greiner part number 381061.

应用

  • 96块深井结晶筛试剂

特征

  • SBS格式,96井
  • 无菌的,单独密封的板
  • 聚丙烯
  • 用铝封II、水晶透明密封膜或帽垫密封

描述

这个Greiner 780261 MASTERBLOCK是一个96深井聚丙烯块(板)。每口井的填充量为1.2ml。MASTERBLOCK适用于在使用液体处理自动化或多通道感应器时保持晶化屏幕试剂。每个盘子是无菌的,单独包装。圆形底井,完全回收试剂。™纯聚丙烯树脂(蒸压至121°C)。SBS推荐尺寸。字母数字编码井与烟囱式,圆形井顶。

这个微板盖是96深井格式结晶屏的临时掩护。在每个试剂井上设置单独的冷凝环的单位置、高剖面覆盖,可以帮助减少96个深井试剂板上的试剂蒸发,并保护试剂免受微粒和污染。该单独包裹,无菌微板盖子是由光学透明的聚苯乙烯。格雷纳656171。

这个帽垫由醋酸乙烯乙酯(EVA)制成。耐DMSO,可在摄氏-20至+60度的温度范围内使用。适用于密封格雷纳780261 1毫升深井板(HR3-105).这些帽垫是不可穿的。供应无菌。格雷纳零件编号381061。

HR3-105 MASTERBLOCK® 96 Deep Well polypropylene plate 50 plate case
HR4-527 Microplate Lid 10 plate case
HR3-103 Cap Mat for MASTERBLOCK® pack of 50

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Hampton HR4-671夹钳-弯 Vial Clamp – Curved, 45°/135°

Hampton HR4-671夹钳-弯 Vial Clamp – Curved, 45°/135°

Applications
Vial support & manipulation
Features
Curved end tip
Hemostat style closure
Description 
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.

应用

  • 小瓶支撑与操纵

特征

  • 弯曲端部
  • 恒流器式关闭

描述

Vial钳-弯是镀铬,止血风格的工具.它有一个尖端形状,当夹子关闭时,可以以一个角度将所有水晶帽系统的存储瓶保持在一个角度上。该夹子可以锁定使用止血方式关闭。夹持小瓶的夹子末端以45°/135°或110°/70°角弯曲。当小瓶放置在夹子中时,它的长度以相同的角度放置在夹子上。每个夹子的总长度是相同的,195毫米。

HR4-671 – Vial Clamp – Curved 45°/135° (front);

HR4-672 – Vial Clamp – Curved 110°/70° (rear)

Hampton HR4-671夹钳-弯 Vial Clamp – Curved, 45°/135°

CAT NO NAME DESCRIPTION
HR4-671 Vial Clamp – Curved 45°/135° each
HR4-672 Vial Clamp – Curved 110°/70° each

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Hampton HR4-733带磁力底座 CrystalCap, with Vial

Hampton HR4-733带磁力底座 CrystalCap, with Vial

CrystalCap™

APPLICATIONS

  • Cryocrystallography

FEATURES

  • SSRL style sample mount for cryocrystallography
  • CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

DESCRIPTION

The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

晶体帽™

应用

  • 冰晶术

特征

  • SSRL型单晶样品架
  • 结晶帽附着在晶体盖和磁干涉仪基座上
  • 与SSRL风格的夹持器、自动装配机和样本处理程序兼容的锥形形状

描述

水晶帽是一个磁性样品安装(也称为盖子,引脚或测角器底座),为接受SSRL型样品安装的晶晶系统设计。晶体帽附着在磁性晶体帽和磁干涉仪基座上。水晶帽的顶端接受装有冷冻环™的微型管™或装有冷冻环™的微管™。

水晶瓶盖是一个1.8毫升的冰瓶,有两个小通风口。将环形磁铁模压到瓶的开口端,以便当瓶盖放置在小瓶中时,环磁铁在低温储存过程中将瓶盖固定在瓶上。HR4-904水晶帽Vial在瓶底没有磁铁。

圆锥形状与平台是兼容的SSRL风格的夹持器,自动装配机和样本处理程序。

与下列同步辐射光束线兼容

北美和南美洲
·高级光源,加州伯克利,ALS 4.2.2,ALS 11.3.1,ALS 12.2.2,ALS 12.3.2
·高级光子源,阿贡,伊利诺伊州APS 14-BM-C生物CARS,APS 14-ID-B生物CARS
·康奈尔高能,同步加速器源,伊萨卡,纽约国际象棋A1,国际象棋F1
加拿大光源,加拿大萨斯喀彻温省,CLS 08ID-1,CLS 08B1-1
·斯坦福同步辐射实验室,加州门罗公园,SSRL BL 7-1,SSRL BL 9-1,SSRL BL 9-2,SSRL BL 11-1,SSRL BL 12-2,SSRL BL 14-1

亚洲和澳大利亚
·上海同步辐射装置,中国上海SSRF BL17U1
·国家同步辐射研究中心,台湾NSRRC BL13B1,NSRRC BL13C1
·波航加速器实验室,韩国POHANG,PAL 2D,PAL 5C,PAL 7A
·光子工厂,筑波,日本PF BL-5A,PF BL-17A,PF AR-NW12A
超级光子环-8 GeV,日本Spring-8 BL12B2,Spring-8 BL24XU,Spring-8 BL26B1,Spring-8 BL26B2,Spring-8 BL32B2,Spring-8 BL32XU,Spring-8 BL38B1,Spring-8 BL41XU,Spring-8 BL44B2,Spring-8 BL44XU

HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial 结晶小瓶 Vial only – 30 pack

Hampton 蛋白结晶试剂–Crystal Screen

Hampton 蛋白结晶试剂–Crystal Screen

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

应用

  • 蛋白质、可溶性肽、核酸和水溶性小分子的初级筛选
  • 稀疏矩阵加性筛

特征

  • 原始稀疏矩阵屏幕
  • 稀疏矩阵公式有效取样盐、聚合物、有机物和pH
  • 对1,000多个生物大分子有效
  • 管式或深井块格式

描述

设计了晶体屏和晶体屏2试剂试剂盒,为大分子结晶提供了一种高效、快速的筛选方法。该筛具有简单实用的特点,可用于寻找初始结晶条件。对1000多个蛋白质、多肽、寡核苷酸和小分子的初始结晶条件进行了晶体筛选。

克服穷尽寻找合适结晶条件的一种非常有效的方法是使用稀疏矩阵试制条件法,这种方法是偏倚的,并从已知的大分子结晶条件中选择。在晶体屏幕和晶体屏幕2中使用的配方评估了96种独特的pH、盐类、聚合物和有机物的混合物,以及它们促进晶体生长的能力。

水晶屏幕包含50种独特的试剂,每个10毫升,是基于稀疏矩阵公式首次描述由Jancarik和Kim在1991年。

水晶屏幕2,一个水晶屏幕的延伸,包含48种独特的试剂,每个10毫升,是基于1994年由Cudney等人首次描述的配方。

晶体屏幕HT包含1ml的每个试剂1-48从水晶屏幕和所有48试剂从水晶屏幕2在一个单一的深井块格式。

准备使用的试剂是无菌过滤和配制超纯1型水,使用最高纯度的盐类,聚合物,有机物和缓冲液.个别试剂可通过汉普顿研究定制商店获得。

Applications

Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules

Sparse matrix additive screen

Features

The original sparse matrix screen

Sparse matrix formula efficiently samples salts, polymers, organics, & pH

Proven effective with more than 1,000 biological macromolecules

Tube or Deep Well block format

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.

Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

CAT NO NAME DESCRIPTION
HR2-110 Crystal Screen 10 ml, tube format
HR2-112 Crystal Screen 2 10 ml, tube format
HR2-130 Crystal Screen HT 1 ml, Deep Well block format

Hampton Research HR2-627 (+/-)-2-Methyl-2,4-pentanediol

Hampton Research HR2-627 (+/-)-2-Methyl-2,4-pentanediol

美国Hampton Research品牌是一家专门从事蛋白质晶体研究的生产商,上海海金畔生物代理销售Hampton蛋白结晶试剂耗材以及工具,欢迎客户Hampton官网或者咨询我们获取更多Hampton产品价格等信息。

APPLICATIONS

  • Crystallization grade (+/-)-2-Methyl-2,4-pentanediol for formulating screens or for optimization

DESCRIPTION

(+/-)-2-Methyl-2,4-pentanediol

Synonyms: MPD or Hexylene glycol
C6H14O2
CH3CH(OH)CH2C(CH3)2OH
Mr 118.18
CAS Number [107-41-5]
EC Number 203-489-0
Beilstein Registry Number 1098298
Merck 14,4710
RTECS SA0810000
MDL Number MFCD00004547
PubChem Substance ID 329760818
Purity ≥ 99.0%
Density 0.925 g/mL at 25°C(lit.)
Boiling point 197.0° C (lit.)
Vapor pressure 0.02 mm Hg at 20°C (lit.)

Measured Conductivity Range: 0.0 – 0.3 µS/cm at 25°C
Measured Refractive Index Range: 1.42725 – 1.42825 at 20°C

HR2-627 100% (+/-)-2-Methyl-2,4-pentanediol 200 mL

REFERENCES

1. Crystallization and Preliminary X-ray Analysis of a Thermophillic Bacillus Xylanase. Pickersgill, RW; Debeire, P; Debeire-Gosselin, M; Jenkins, JA. J Mol Biol 230, 664- 666, 1993.