Hampton HR2-539 四水酒石酸钾钠缓冲液

Hampton 四水酒石酸钾钠缓冲液

Potassium sodium tartrate tetrahydrate  四水酒石酸钾钠

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

APPLICATIONS 应用

  • Crystallization grade Potassium sodium tartrate tetrahydrate for formulating screens or for optimization 四水酒石酸钾结晶级筛选或优化

FEATURES  特征

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤液
  • 在1+超纯水中配制:在25°C下电阻率为18.2兆,总有机碳<5 ppb,无细菌(<1细菌(CFU/ml)),无热原(<0.03内毒素(EU/ml)),无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

DESCRIPTION

Potassium sodium tartrate tetrahydrate

Synonyms: L(+)-Tartaric acid potassium sodium salt, or Rochelle salt, or Seignette salt
KOCOCH(OH)CH(OH)COONa • 4H2O
C4H4KNaO• 4H2O
Mr 282.22
CAS Number [6381-59-5]
EC Number 206-156-8
Beilstein Registry Number 6113568
MDL Number MFCD00150989
PubChem Substance ID 24881874
Purity ≥ 99.5%

Measured pH Range: 8.4 – 9.1 at 25°C
Measured Conductivity Range: 81.1 – 98.5 mS/cm at 25°C
Measured Refractive Index Range: 1.37544 – 1.37666 20°C

描述

四水酒石酸钾钠

同义词:l(+)-酒石酸钾钠盐,或罗谢尔盐,或塞格奈特盐
KOCOCH(OH)CH(OH)Coona·4H2O
C4H4KNaO·4H2O
Mr 282.22
核证机关编号[6381-59-5]
欧共体编号206-156-8
伯尔斯坦登记处编号6113568
MDL号码MFCD 00150989
PubChem物质ID 24881874
纯度≥99.5%

测量pH值范围:8.4-9.1℃,25°C
测量电导率范围:81.1-98.5ms/cm,25°C
测量折射率范围:1.37544-1.37666 20°C

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
N ≤0.002%
Ni ≤0.0005%
Pb ≤0.0005%
PO4 ≤0.001%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

HR2-539 1.5 M Potassium sodium tartrate tetrahydrate 200 mL

 

REFERENCES 参考文献

1. Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3. K. Miyazono, N. Kudo and M. Tanokura. Acta Cryst. (2004). D60, 1135-1136.

2. Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides. Johansson P, Denman S, Brumer H, Kallas AM, Henriksson H, Bergfors T, Teeri TT, Jones TA. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):535-7.

Hampton Research HR2-627 (+/-)-2-Methyl-2,4-pentanediol

Hampton Research HR2-627 (+/-)-2-Methyl-2,4-pentanediol

美国Hampton Research品牌是一家专门从事蛋白质晶体研究的生产商,上海海金畔生物代理销售Hampton蛋白结晶试剂耗材以及工具,欢迎客户Hampton官网或者咨询我们获取更多Hampton产品价格等信息。

APPLICATIONS

  • Crystallization grade (+/-)-2-Methyl-2,4-pentanediol for formulating screens or for optimization

DESCRIPTION

(+/-)-2-Methyl-2,4-pentanediol

Synonyms: MPD or Hexylene glycol
C6H14O2
CH3CH(OH)CH2C(CH3)2OH
Mr 118.18
CAS Number [107-41-5]
EC Number 203-489-0
Beilstein Registry Number 1098298
Merck 14,4710
RTECS SA0810000
MDL Number MFCD00004547
PubChem Substance ID 329760818
Purity ≥ 99.0%
Density 0.925 g/mL at 25°C(lit.)
Boiling point 197.0° C (lit.)
Vapor pressure 0.02 mm Hg at 20°C (lit.)

Measured Conductivity Range: 0.0 – 0.3 µS/cm at 25°C
Measured Refractive Index Range: 1.42725 – 1.42825 at 20°C

HR2-627 100% (+/-)-2-Methyl-2,4-pentanediol 200 mL

REFERENCES

1. Crystallization and Preliminary X-ray Analysis of a Thermophillic Bacillus Xylanase. Pickersgill, RW; Debeire, P; Debeire-Gosselin, M; Jenkins, JA. J Mol Biol 230, 664- 666, 1993.

Hampton HR2-100 StockOptions Tris Buffer Kit

Hampton HR2-100 StockOptions Tris Buffer Kit

美国Hampton Research品牌是一家专门从事蛋白质晶体研究的生产商,上海海金畔生物代理销售Hampton蛋白结晶试剂耗材以及工具,欢迎客户Hampton官网或者咨询我们获取更多Hampton产品价格等信息。

APPLICATIONS

  • Focused, titrated buffer stocks for crystal screening, optimization, & production

FEATURES

  • pH 7.0 – 9.0 (21 reagents)
  • 0.1 pH increments
  • 1.0 M concentrated stocks
  • Tris pKa = 8.1

DESCRIPTION

StockOptions Tris buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The StockOptions Tris buffer stock reagents are supplied as 1.0 M stock solutions in 10 ml volumes. Each StockOptions Tris buffer reagent is carefully titrated using hydrochloric acid. StockOptions Tris is comprised of 21 unique reagents covering the pH range of 7.0 to 9.0 in 0.1 pH unit increments.

Tris
Synonym: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3 NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity =99.8%
pKa = 8.1 at 25°C
Useful pH range of Tris = 7.0 – 9.0

Hampton HR2-428 Additive Screen • Additive Screen HT

Hampton HR2-428 Additive Screen • Additive Screen HT

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

APPLICATIONS  应用

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

操纵样品-样品和样品-溶剂相互作用以改善晶体或改变样品溶解度

FEATURES  特征

  • 18 classes of reagents
  • Highly concentrated (10x) reagent formulation
  • 96 unique reagents and excipients
  • Tube or Deep Well block format
  • Crystallization or sample solubility optimization

18类试剂
高浓度 (10x) 试剂配方
96 种独特的试剂和赋形剂
管或深井块格式
结晶或样品溶解度优化

DESCRIPTION  描述

Additive Screen is a library of reagents that can affect the solubility and crystallization of biological macromolecules, including both soluble and membrane proteins.

These reagents can perturb and manipulate sample-sample and sample-solvent interactions, as well as perturb water structure which can alter and improve both the solubility and crystallization of a sample. Additives can stabilize or engender conformity by specific interaction with the macromolecules. There are numerous reports of the use of additives to improve the quality and size of macromolecular crystals.1-5

Additive screen reagent classifications include multivalent, salt, amino acid, dissociating agent, linker, polyamine, osmolyte, chaotrope, co-factor, reducing agent, chelating agent, polymer, carbohydrate, non-detergent, amphiphile, detergent, non-volatile organic and volatile organic.

Additive Screen contains 96 unique reagents, 1 ml each.

Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block.

Additive Screen 是一个试剂库,可以影响生物大分子的溶解度和结晶,包括可溶性蛋白和膜蛋白。

这些试剂可以干扰和操纵样品-样品和样品-溶剂的相互作用,以及可以改变和改善样品溶解度和结晶的水结构。添加剂可以通过与大分子的特定相互作用来稳定或产生一致性。有许多关于使用添加剂来改善大分子晶体的质量和尺寸的报道。 1-5

添加剂筛选试剂分类包括多价、盐、氨基酸、解离剂、连接剂、多胺、渗透剂、离液剂、辅助因子、还原剂、螯合剂、聚合物、碳水化合物、非去污剂、两亲物、去污剂、非挥发性有机物和挥发性有机物。

Additive Screen 包含 96 种独特的试剂,每种试剂 1 ml。

Additive Screen HT 在单个 Deep Well 模块中包含 96 种独特的试剂,每种试剂 1 ml。

HR2-428 Additive Screen 1 ml, tube format
HR2-138 Additive Screen HT 1 ml, Deep Well block format

Hampton HR2-122 Crystal Screen Cryo

Hampton HR2-122 Crystal Screen Cryo
Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS

  • Primary biased sparse matrix screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules.

FEATURES

  • Developed at Hampton Research
  • Cryo ready screening reagents for cryocrystallography
  • Sparse matrix formula efficiently samples salts, polymers, organics, & pH
  • Tube or Deep Well block format
  • Screen for cryo and crystallization conditions using only one screen

DESCRIPTION

Determining initial and optimal cryoprotectant concentration is often a process of trial and error. One must find suitable cryoprotectant concentrations which stabilize the crystal while at the same time combine with the crystallization reagent to form an amorphous glass.

The Crystal Screen Cryo kits removes the guess work from determining the preliminary glycerol concentration required to mix with the crystallization reagent to form an amorphous glass. Crystal Screen Cryo reagents are formulated with the appropriate amount of glycerol required to form an amorphous glass with each unique reagent composition. Crystals obtained in Crystal Screen Cryo kits will already have a suitable concentration of glycerol in the reagent. This avoids exhaustive empirical searches for preliminary cryoprotectant conditions. From the preliminary cryoprotectant conditions, cryoprotectant concentration can be optimized to minimize mosaic spread and maximize diffraction resolution.

Crystal Screen Cryo kits are biased sparse matrices designed for rapid screening of crystallization conditions of biological macromolecules in the presence of glycerol, the most frequently utilized cryoprotectant. The Crystal Screen Cryo kits utilize the original Crystal Screen™ and Crystal Screen 2™ protocol optimized to include the appropriate concentration of glycerol required to form an amorphous glass at 100°K. The primary screen variables are salt, pH, precipitant (salts, polymers, volatile organics, and non-volatile organics), and cryoprotectant concentration. The screen is a straightforward, effective, and practical kit for determining preliminary crystallization conditions and provides a good starting point for finding suitable cryoprotectant conditions for macromolecular crystals grown in a wide range of reagents. Crystal Screen Cryo is also effective in determining the solubility of a macromolecule in a wide range of precipitants and pH.

Crystal Screen Cryo contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991 and refined for cryo by Garman in 1996.

Crystal Screen 2 Cryo contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994 and refined for cryo by Snell et al in 2002.

Crystal Screen Cryo HT contains 1 ml each of reagents 1-48 from Crystal Screen Cryo and 48 reagents from Crystal Screen 2 Cryo in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers.

HR2-122 Crystal Screen Cryo 10 ml, tube format
HR2-121 Crystal Screen 2 Cryo 10 ml, tube format
HR2-133 Crystal Screen Cryo HT 1 ml, Deep Well block format

Hampton HR2-597 Jeffamine® ED-2001 pH 7.0

Hampton HR2-597 Jeffamine® ED-2001 pH 7.0

APPLICATIONS

  • Crystallization grade Jeffamine ED-2001 pH 7.0 for formulating screens or for optimization

FEATURES

  • pH adjusted to 7 using HCl
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

Jeffamine ED series are polyether diamines based on a predominantly PEG backbone. PEG imparts complete water solubility. The Jeffamine® ED-2001 have a representative structure illustrated in the accompanying Figure.

Jeffamine® ED-2001
Synonym: O,O′-Bis(2-aminopropyl) polypropylene glycol-block-polyethylene glycol-block-polypropylene glycol 1,900, Jeffamine® ED-2003
Mr ~2,000
CH3CH(NH2)CH2(OCH2CH2)nOCH2CH(NH2)CH3
CAS Number [65605-36-9]
MDL Number MFCD00197920
PubChem Substance ID 24848714
pH before titration with hydrochloric acid is > 10
Final measured pH of HR2-597 is 7.0
Appearance: Colorless to Very light yellow tint to yellow, clear solution
50% w/v in deionized water, sterile filtered (0.22 micron)

Measured Conductivity Range: 4.0 – 5.7 mS/cm at 25°C
Measured Refractive Index Range: 1.40572 – 1.40725 at 20°C

Jeffamine is a registered trademark of the Huntsman Petrochemical Corporation.

CAT NO NAME DESCRIPTION
HR2-597 50% w/v Jeffamine  ED-2001 pH 7.0 200 mL

Hampton 聚乙二醇单甲醚 Polyethylene glycol monomethyl ether 2,000

Hampton 聚乙二醇单甲醚 Polyethylene glycol monomethyl ether 2,000

APPLICATIONS

  • Crystallization grade Polyethylene glycol monomethyl ether 2,000 for formulating screens or for optimization

FEATURES

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

Polyethylene glycol monomethyl ether 2,000

Synonyms: Methoxy poly(ethylene glycol) 2,000 or mono-Methyl polyethylene glycol 2,000 or PEG MME 2,000, or MPEG
CH3O(CH2CH2O)nH
Formula Weight (Mw) ~ 2,000
CAS Number [9004-74-4]
EC Number 215-801-2
RTECS PE8271000
MDL Number MFCD00084416
Melting Point (starting material) 52-56°C
Appearance (Solution): Colorless to Light Yellow to Light Brown, Clear

Aldehyde None detected
Peroxide None detected

Measured Conductivity Range: 28.0 – 60.2 µS/cm at 25°C
Measured Refractive Index Range: 1.40105 – 1.40205 at 20°C

Sterile filtered solution (0.22 micron) in sterile PETG bottle, purged with dry argon

CAT NO NAME DESCRIPTION
HR2-613 50% w/v Polyethylene glycol monomethyl ether 2,000 200 mL

Hampton热销产品

Hampton热销产品

Hampton Research

在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton高品质的产品和服务,欢迎广大新老客户来电咨询。‘

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域受信赖的品牌之一。

Hampton research主要产品:

Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液

Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件

Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops

Goniometer Heads&Supplies 测角器头和配件

Protein Crystallization Standards 结晶级别标准蛋白

Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分

StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒

Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具

Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton热销产品

  • StockOptions pH Buffer Kit HR2-241Crystallization grade buffer stocks for crystal screening, optimization, & productionCrystal Screen Cryo HR2-122Primary screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules
  • Natrix HR2-116Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes
  • MembFac HR2-114Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility
  • SaltRx HR2-136Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
  • Grid Screens HR2-248Primary or secondary, salt, polymer, organic and pH grid crystallization screen for biological macromolecules
  • PEG/Ion HR2-126Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
  • PCT™ Pre-Crystallization Test HR2-140Determine the appropriate sample concentration for crystallization screening
  • Index HR2-144Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules
  • Crystal Screen HR2-110Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules;Sparse matrix additive screen
  • PEGRx HR2-082Primary and secondary, polymer and pH based crystallization screen for biological macromolecules
  • Additive Screen HR2-428Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
  • Detergent Screen HR2-408Prevent and manipulate non-specific aggregation due to hydrophobic interactions
  • CryoPro HR2-073Water soluble cryoprotectant reagent set designed for the cryopreservation of biological macromolecular crystals
  • StockOptions Salt HR2-245Crystallization grade salt reagent stock solutions for screen formulation and optimization
  • Siliconized Glass Cover Slides HR3-217Hanging, sitting or sandwich drop crystallization
  • Intelli-Plate 96 HR3-185蛋白结晶座滴板
  • CrystalCap HR4-733
  • CryoLoop HR4-623

    • CryoTong™ – Long HR5-104Crystal transfer under cryo temperature
    • CrystalWand™ Magnetic HR4-729CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool
    • Vial Clamp™ – Straight HR4-670Vial support and manipulation
    • CrystalCap™ Holder HR4-707
    • CryoCane HR4-709

      Organized storage of CrystalCaps

Hampton蛋白结晶产品目录

Hampton蛋白结晶产品目录

产品编号 中文品名 英文品名
HR4-126 11 刀片替换装 Replacement #11 Blade
HR4-904 CrystalCap Magnetic Vial CrystalCap™, Vial only
HR4-729 CrystalWand Magnetic, Straight CrystalWand™ Magnetic, Straigh
HR4-837 Micro-Tools II Set Micro-Tools II™ Set
HR4-811 Micro-Tools Set Micro-Tools™ Set
HR3-172 VDX 24孔板,带密封剂 VDX™ Plate, with sealant
HR4-902 带磁力底座 CrystalCap™, without Vial
HR4-733 带磁力底座 CrystalCap™, with Vial
HR4-745 带铜管磁力底座 CrystalCap™ Copper 18 mm, with
HR4-708 冻存套 CryoSleeve™
HR3-217 硅化方形盖玻片22mmx0.22mm 22 mm x 0.22 mm Siliconized Sq
HR4-671 夹钳-弯 Vial Clamp™ – Curved, 45°/135°
HR4-670 夹钳-直 Vial Clamp™ – Straight
HR4-316 Super Glue
HR4-506 胶带 3 inch wide x 55 yard Crystal
HR3-083 结晶板 2孔 MRC 2 Well Crystallization Pla
HR3-125 结晶板 3孔 3 Well Crystallization Plate (
HR4-217 晶体探针 Crystal Probe
HR4-662 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-673 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-674 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-675 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR1-008 液氮罐 CX100 (含运输箱) CX100 Cryo-Express Dry Shipper
HR2-912-37 Crystal Screen 2™ #37
HR2-501 50% v/v Jeffamine® M-600® pH 7
HR2-509 1.0 M BICINE
HR2-517 100% Polyethylene glycol 300
HR2-523 50% w/v Polyethylene glycol 1,
HR2-525 50% w/v Polyethylene glycol 1,
HR2-529 50% w/v Polyethylene glycol 4,
HR2-533 50% w/v Polyethylene glycol 6,
HR2-537 1.0 M Magnesium formate dihydr
HR2-541 3.5 M Ammonium sulfate
HR2-545 2.0 M Lithium sulfate monohydr
HR2-547 7.0 M Sodium formate
HR2-549 1.6 M Sodium citrate tribasic
HR2-551 4.0 M Sodium phosphate monobas
HR2-557 2.0 M Calcium chloride dihydra
HR2-563 1.0 M Zinc acetate dihydrate
HR2-102 StockOptions™ Hepes
HR2-103 StockOptions™ Bis-Tris Propane
HR2-104 StockOptions™ Citric Acid
HR2-106 StockOptions™ Bis-Tris
HR2-107 SaltRx™ 1
HR2-109 SaltRx™ 2
HR2-110 Crystal Screen™
HR2-112 Crystal Screen 2™
HR2-114 MembFac™
HR2-116 Natrix™
HR2-117 Natrix™ 2
HR2-118 Nucleic Acid Mini Screen™
HR2-126 PEG/Ion Screen™
HR2-235 StockOptions™ Sodium Citrate
HR2-237 StockOptions™ Tris Hydrochlori
HR2-239 StockOptions™ Sodium Cacodylat
HR2-241 StockOptions™ pH
HR2-245 StockOptions™ Salt
HR2-249 StockOptions™ Succinic Acid
HR2-320 Seed Bead™
HR2-406 Detergent Screen HT™
HR2-428 Additive Screen™
HR2-434 Reductive Alylation Kit
HR2-442 Heavy Atom Screen™ Pt
HR2-444 Heavy Atom Screen™ Au
HR2-446 Heavy Atom Screen™ Hg
HR2-448 Heavy Atom Screen M1™
HR2-450 Heavy Atom Screen M2™
HR2-082 PEGRx™ 1
HR2-084 PEGRx™ 2
HR2-096 Silver Bullets™
HR2-098 PEG/Ion 2 Screen™
HR4-315 CrystalWand™ Magnetic, 45° McM
HR4-350 Large Applicator (200 & 350 µl
HR4-625 Mounted CryoLoop™ – 20 micron,
HR4-637 CryoTong™ 18 mm (standard 110
HR4-709 CryoCane™ 5 Vial Holder
HR4-711 CryoCane™ 6 Vial Holder
HR2-211 Grid Screen™ Ammonium Sulfate
HR2-213 Grid Screen™ PEG 6000
HR2-227 StockOptions™ Polymer
HR2-233 StockOptions™ Sodium Acetate
HR4-931 CryoLoop™ – 20 micron, 0.2 – 0
HR4-945 Mounted CryoLoop™ – 20 micron,
HR4-947 Mounted CryoLoop™ – 20 micron,
HR4-953 Mounted CryoLoop™ Sampler – 20
HR4-955 Mounted CryoLoop™ – 20 micron,
HR4-957 Mounted CryoLoop™ – 20 micron,
HR4-959 Mounted CryoLoop™ – 20 micron,
HR4-961 Mounted CryoLoop™ – 20 micron,
HR4-963 Mounted CryoLoop™ – 20 micron,
HR4-970 Mounted CryoLoop™ – 20 micron,
HR4-971 Mounted CryoLoop™ – 20 micron,
HR4-989 CryoLoop™ – 10 micron, 0.3 – 0
HR2-128 Crystal Screen Lite™
HR2-138 Additive Screen HT™
HR2-140 PCT™ – Pre-Crystallization T
HR4-313 Mounted CryoLoop™ – 20 micron,
HR2-601 100% Polyethylene glycol 200
HR2-607 50% w/v Polyethylene glycol 10
HR2-609 30% w/v Polyethylene glycol 20
HR2-611 100% Polyethylene glycol monom
HR2-613 50% w/v Polyethylene glycol mo
HR2-615 50% w/v Polyethylene glycol mo
HR2-625 6.0 M 1,6-Hexanediol
HR2-637 5.0 M Sodium chloride
HR2-723 1.0 M BICINE pH 9.0
HR2-755 100% Tacsimate™ pH 7.0
HR2-761 3.0 M DL-Malic acid pH 7.0
HR2-801 TCEP hydrochloride
HR2-821 2.0 M Magnesium sulfate heptah
HR2-829 100% Tacsimate™ pH 8.0
HR2-857 4.0 M TMAO
HR2-859 100% Polyethylene glycol 600
HR2-910-08 Crystal Screen™ #08
HR2-144 Index™
HR4-993 Mounted CryoLoop™ Sampler – 10
HR4-995 Mounted CryoLoop™ – 10 micron,
HR4-999 Mounted CryoLoop™ – 10 micron,
HR6-146 1.0 mm Quartz Capillary
HR8-034 Cryo Puck Carrier
HR8-035 Porcupine Push Tool
HR8-036 Puck Wand
HR8-037 Cryo Puck Base Removal Platfor
HR8-038 Angled Cryo Puck Tong
HR8-039 Cryo Puck Dewar Loading Tool
HR8-114 CrystalCap HT™, Vial only
HR8-133 Seeding Tool
HR8-173 CrystalCap™ Copper HT, 0.025 –
HR8-174 CrystalCap™ Copper HT, 0.05 –
HR8-175 CrystalCap™ Copper HT, 0.1 – 0
HR8-176 CrystalCap™ Copper HT, 0.2 – 0
HR2-575 1.0 M Sodium cacodylate trihyd
HR2-581 1.0 M Hydrochloric acid

Hampton 透析按钮 Dialysis Buttons

Hampton 透析按钮 Dialysis Buttons

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Hampton 透析按钮 Dialysis Buttons

Applications
Crystallization by dialysis, protein folding, & small volume sample dialysis

Features
Low volume dialysis
Fits in 24 well plates
Use over and over again

Methodologies
Controlled crystal dehydration.4

Description 
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3 Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

应用

透析结晶,蛋白质折叠,小容量样品透析

特征

低容量透析
适用于24孔板
一遍又一遍地使用

方法

控制晶体脱水
描述 
在透析法中,样品被半透膜从“沉淀剂”中分离出来,这种膜允许小的试剂分子通过,但防止生物大分子穿过膜。1-3透析按钮是注射成型的聚苯乙烯,大小与一个小按钮。样品被放置在这个房间,以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部,并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米,外径约为10毫米而没有O形环,以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米,外径约为15毫米,没有O形环,而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头,以协助将透析膜应用到按钮上,并与所提供的o-环固定在一起。

Hampton 透析按钮 Dialysis Buttons

CAT NO NAME DESCRIPTION
HR3-336 Dialysis Buttons Sampler 5 of each size listed below
HR3-314 5 µl Dialysis Button 50 pack
HR3-316 10 µl Dialysis Button 50 pack
HR3-318 15 µl Dialysis Button 50 pack
HR3-320 20 µl Dialysis Button 50 pack
HR3-326 50 µl Dialysis Button 50 pack
HR3-328 100 µl Dialysis Button 50 pack
HR3-330 200 µl Dialysis Button 50 pack
HR3-332 350 µl Dialysis Button 50 pack

1 Related Item  相关产品信息
Applicator for Dialysis Buttons

References  参考文献
1.McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2.Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3.Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4.Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.