标签归档：Hampton蛋白结晶工具

Hampton HR4-126 X-Acto Gripster Knife 密封胶带和薄膜割刀

发表于由

Hampton HR4-126 X-Acto Gripster Knife 密封胶带和薄膜割刀

APPLICATIONS

  • Cutting and removing sealing tape and film from crystallization plates

FEATURES

  • Sharp stainless steel blade
  • Safety cap for blade
  • Soft grip barrel
  • Anti-roll design – will not roll off table or microscope

DESCRIPTION

Precisely cuts tapes and films around reservoir and drops from crystallization plates, safely and easily. Ergonomic soft rubberized barrel is firm and comfortable for better control. Easy to clean handle. Rear blade release is for easier and safer blade changing. Rear blade release makes blade changing safer, easier, and faster. Safety cap cover for blade. Anti-roll stop design – knife will not roll off table or microscope and stab you in the toe. Supplied with one #11 blade. Replacement blades available separately.

#11 Replacement Blade – Extremely sharp point for fine angle cutting. Stainless steel. Sold in a package of five blades.

X-Acto® is a registered trademark of Elmer’s Products, Inc.

CAT NO

HR4-124     X-Acto Gripster Knife     Each

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents单独成分

发表于由

Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents单独成分

APPLICATIONS

  • Individual reagents from SaltRx 1, SaltRx 2, and SaltRx HT for screening, optimization and production

FEATURES

  • 185 ml volume
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

HR2-907-** and HR2-909-**
** Refers to the reagent number in the kit.
For example, SaltRx 1 reagent number 1 = SaltRx HT reagent A1 = HR2-907-01
For example, SaltRx 2 reagent number 1 = SaltRx HT reagent E1 = HR2-909-01

CAT NO

HR2-907-01

NAME

SaltRx 1 #01 / SaltRx HT A1 185ml

DESCRIPTION

1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-02

NAME

SaltRx 1 #02 / SaltRx HT A2 185ml

DESCRIPTION

2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-03

NAME

SaltRx 1 #03 / SaltRx HT A3 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-04

NAME

SaltRx 1 #04 / SaltRx HT A4 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-05

NAME

SaltRx 1 #05 / SaltRx HT A5 185ml

DESCRIPTION

1.5 M Ammonium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-06

NAME

SaltRx 1 #06 / SaltRx HT A6 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-07

NAME

SaltRx 1 #07 / SaltRx HT A7 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-08

NAME

SaltRx 1 #08 / SaltRx HT A8 185ml

DESCRIPTION

3.5 M Ammonium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-09

NAME

SaltRx 1 #09 / SaltRx HT A9 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-10

NAME

SaltRx 1 #10 / SaltRx HT A10 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-11

NAME

SaltRx 1 #11 / SaltRx HT A11 185ml

DESCRIPTION

2.2 M Sodium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-12

NAME

SaltRx 1 #12 / SaltRx HT A12 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-13

NAME

SaltRx 1 #13 / SaltRx HT B1 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-14

NAME

SaltRx 1 #14 / SaltRx HT B2 185ml

DESCRIPTION

3.2 M Sodium chloride, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-15

NAME

SaltRx 1 #15 / SaltRx HT B3 185ml

DESCRIPTION

1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-16

NAME

SaltRx 1 #16 / SaltRx HT B4 185ml

DESCRIPTION

1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-17

NAME

SaltRx 1 #17 / SaltRx HT B5 185ml

DESCRIPTION

1.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-18

NAME

SaltRx 1 #18 / SaltRx HT B6 185ml

DESCRIPTION

2.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-19

NAME

SaltRx 1 #19 / SaltRx HT B7 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-20

NAME

SaltRx 1 #20 / SaltRx HT B8 185ml

DESCRIPTION

0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-21

NAME

SaltRx 1 #21 / SaltRx HT B9 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-22

NAME

SaltRx 1 #22 / SaltRx HT B10 185ml

DESCRIPTION

1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-23

NAME

SaltRx 1 #23 / SaltRx HT B11 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-24

NAME

SaltRx 1 #24 / SaltRx HT B12 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-25

NAME

SaltRx 1 #25 / SaltRx HT C1 185ml

DESCRIPTION

0.4 M Magnesium formate dihydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-26

NAME

SaltRx 1 #26 / SaltRx HT C2 185ml

DESCRIPTION

0.7 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-27

NAME

SaltRx 1 #27 / SaltRx HT C3 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-28

NAME

SaltRx 1 #28 / SaltRx HT C4 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-29

NAME

SaltRx 1 #29 / SaltRx HT C5 185ml

DESCRIPTION

2.0 M Sodium formate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-30

NAME

SaltRx 1 #30 / SaltRx HT C6 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-31

NAME

SaltRx 1 #31 / SaltRx HT C7 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-32

NAME

SaltRx 1 #32 / SaltRx HT C8 185ml

DESCRIPTION

3.5 M Sodium formate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-33

NAME

SaltRx 1 #33 / SaltRx HT C9 185ml

DESCRIPTION

1.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-34

NAME

SaltRx 1 #34 / SaltRx HT C10 185ml

DESCRIPTION

2.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-35

NAME

SaltRx 1 #35 / SaltRx HT C11 185ml

DESCRIPTION

1.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-36

NAME

SaltRx 1 #36 / SaltRx HT C12 185ml

DESCRIPTION

2.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-37

NAME

SaltRx 1 #37 / SaltRx HT D1 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-38

NAME

SaltRx 1 #38 / SaltRx HT D2 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-39

NAME

SaltRx 1 #39 / SaltRx HT D3 185ml

DESCRIPTION

2.5 M Ammonium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-40

NAME

SaltRx 1 #40 / SaltRx HT D4 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-41

NAME

SaltRx 1 #41 / SaltRx HT D5 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-42

NAME

SaltRx 1 #42 / SaltRx HT D6 185ml

DESCRIPTION

6.0 M Ammonium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-43

NAME

SaltRx 1 #43 / SaltRx HT D7 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-44

NAME

SaltRx 1 #44 / SaltRx HT D8 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-45

NAME

SaltRx 1 #45 / SaltRx HT D9 185ml

DESCRIPTION

1.5 M Sodium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-907-46

NAME

SaltRx 1 #46 / SaltRx HT D10 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-907-47

NAME

SaltRx 1 #47 / SaltRx HT D11 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-907-48

NAME

SaltRx 1 #48 / SaltRx HT D12 185ml

DESCRIPTION

4.0 M Sodium nitrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-01

NAME

SaltRx 2 #01 / SaltRx HT E1 185ml

DESCRIPTION

1.0 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-02

NAME

SaltRx 2 #02 / SaltRx HT E2 185ml

DESCRIPTION

1.8 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-03

NAME

SaltRx 2 #03 / SaltRx HT E3 185ml

DESCRIPTION

1.5 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-04

NAME

SaltRx 2 #04 / SaltRx HT E4 185ml

DESCRIPTION

2.4 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-05

NAME

SaltRx 2 #05 / SaltRx HT E5 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 (0.98 M Sodium phosphate monobasic monohydrate, 0.02 M Potassium phosphate dibasic)

 CAT NO

HR2-909-06

NAME

SaltRx 2 #06 / SaltRx HT E6 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 (0.35 M Sodium phosphate monobasic monohydrate, 0.65 M Potassium phosphate dibasic)

 CAT NO

HR2-909-07

NAME

SaltRx 2 #07 / SaltRx HT E7 185ml

DESCRIPTION

1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 (0.04 M Sodium phosphate monobasic monohydrate, 0.96 M Potassium phosphate dibasic)

 CAT NO

HR2-909-08

NAME

SaltRx 2 #08 / SaltRx HT E8 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 (1.764 M Sodium phosphate monobasic monohydrate, 0.036 M Potassium phosphate dibasic)

 CAT NO

HR2-909-09

NAME

SaltRx 2 #09 / SaltRx HT E9 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 (0.63 M Sodium phosphate monobasic monohydrate, 1.17 M Potassium phosphate dibasic)

 CAT NO

HR2-909-10

NAME

SaltRx 2 #10 / SaltRx HT E10 185ml

DESCRIPTION

1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 (0.072 M Sodium phosphate monobasic monohydrate, 1.728 M Potassium phosphate dibasic)

 CAT NO

HR2-909-11

NAME

SaltRx 2 #11 / SaltRx HT E11 185ml

DESCRIPTION

0.5 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-12

NAME

SaltRx 2 #12 / SaltRx HT E12 185ml

DESCRIPTION

1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-13

NAME

SaltRx 2 #13 / SaltRx HT F1 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-14

NAME

SaltRx 2 #14 / SaltRx HT F2 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-15

NAME

SaltRx 2 #15 / SaltRx HT F3 185ml

DESCRIPTION

1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-16

NAME

SaltRx 2 #16 / SaltRx HT F4 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-17

NAME

SaltRx 2 #17 / SaltRx HT F5 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-18

NAME

SaltRx 2 #18 / SaltRx HT F6 185ml

DESCRIPTION

2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-19

NAME

SaltRx 2 #19 / SaltRx HT F7 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-20

NAME

SaltRx 2 #20 / SaltRx HT F8 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-21

NAME

SaltRx 2 #21 / SaltRx HT F9 185ml

DESCRIPTION

0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-22

NAME

SaltRx 2 #22 / SaltRx HT F10 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-23

NAME

SaltRx 2 #23 / SaltRx HT F11 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-24

NAME

SaltRx 2 #24 / SaltRx HT F12 185ml

DESCRIPTION

1.5 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-25

NAME

SaltRx 2 #25 / SaltRx HT G1 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-26

NAME

SaltRx 2 #26 / SaltRx HT G2 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-27

NAME

SaltRx 2 #27 / SaltRx HT G3 185ml

DESCRIPTION

1.0 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-28

NAME

SaltRx 2 #28 / SaltRx HT G4 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-29

NAME

SaltRx 2 #29 / SaltRx HT G5 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-30

NAME

SaltRx 2 #30 / SaltRx HT G6 185ml

DESCRIPTION

1.8 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-31

NAME

SaltRx 2 #31 / SaltRx HT G7 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-32

NAME

SaltRx 2 #32 / SaltRx HT G8 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-33

NAME

SaltRx 2 #33 / SaltRx HT G9 185ml

DESCRIPTION

0.7 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-34

NAME

SaltRx 2 #34 / SaltRx HT G10 185ml

DESCRIPTION

1.0 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-35

NAME

SaltRx 2 #35 / SaltRx HT G11 185ml

DESCRIPTION

1.3 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-36

NAME

SaltRx 2 #36 / SaltRx HT G12 185ml

DESCRIPTION

1.4 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-37

NAME

SaltRx 2 #37 / SaltRx HT H1 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-38

NAME

SaltRx 2 #38 / SaltRx HT H2 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-39

NAME

SaltRx 2 #39 / SaltRx HT H3 185ml

DESCRIPTION

0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-40

NAME

SaltRx 2 #40 / SaltRx HT H4 185ml

DESCRIPTION

1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-41

NAME

SaltRx 2 #41 / SaltRx HT H5 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-42

NAME

SaltRx 2 #42 / SaltRx HT H6 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-43

NAME

SaltRx 2 #43 / SaltRx HT H7 185ml

DESCRIPTION

0.5 M Potassium thiocyanate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-44

NAME

SaltRx 2 #44 / SaltRx HT H8 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6

 CAT NO

HR2-909-45

NAME

SaltRx 2 #45 / SaltRx HT H9 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-46

NAME

SaltRx 2 #46 / SaltRx HT H10 185ml

DESCRIPTION

4.0 M Ammonium acetate, 0.1 M Tris pH 8.5

 CAT NO

HR2-909-47

NAME

SaltRx 2 #47 / SaltRx HT H11 185ml

DESCRIPTION

35% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

 CAT NO

HR2-909-48

NAME

SaltRx 2 #48 / SaltRx HT H12 185ml

DESCRIPTION

60% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0

Hampton 带磁力结晶环(ALS型)底座 CrystalCap™ ALS HT

发表于由

Hampton 带磁力结晶环(ALS型)底座 CrystalCap™ ALS HT

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
  • 2D alphanumeric coding for sample tracking & management
  • Color coded CryoLoop size

DESCRIPTION

The CrystalCap ALS HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS HT attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS HT is compatible with ALS style grippers, auto mounters and sample handlers.

The CrystalCap ALS HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

Note: CrystalCap ALS HT with Mounted CryoLoops are sold without vials. Vials HR4-904 sold separately.

Color Coded Cap…………CryoLoop Size
Red…………………………….0.025-0.05 mm
Green…………………………….0.05-0.1 mm
Blue………………………………..0.2-0.3 mm
Blue/Red………………………….0.3-0.4 mm
Green/Red………………………..0.4-0.5 mm
Yellow/Green……………………..0.7-1.0 mm

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

结晶帽™ALS HT

应用

  • 冰晶术

特征

  • AlS型结晶架
  • 结晶帽ALS HT磁性附着在结晶帽Vial磁力仪基座
  • 可与ALS风格的夹持器、自动装配器和示例处理程序兼容的自由锥形形状
  • 用于样本跟踪和管理的二维字母数字编码
  • 彩色编码冷冻环尺寸

描述

结晶帽ALS HT是一个磁性样品安装(也称为盖子，引脚或测角器底座)，为接受ALS型样品安装的冰晶系统设计。结晶帽ALS HT附着在一个磁晶帽和磁测角仪的基座上。水晶帽的顶端接受安装的冷冻环™。

水晶瓶盖是一个1.8毫升的冰瓶，有两个小通风口。将环形磁铁模压到瓶的开口端，以便当盖被放置在瓶中时，环磁铁在低温储存期间将盖保持在瓶上。HR4-904水晶帽瓶底部没有磁铁。

水晶帽ALS HT的无棱镜锥形形状与ALS风格的夹持器、自动装配机和样品处理程序兼容。

水晶帽ALS HT的特点是一个二维字母数字16×16数据矩阵代码在帽子的底部。每个帽子的颜色也编码为Cryoloop大小。

注：水晶帽ALS HT与安装超低温是出售没有小瓶。小瓶HR4-904单独出售.

颜色编码，第.章，冷冻环尺寸
Red..0.025-0.05 mm
Green..0.05-0.1 mm
Blue..0.2-0.3 mm
Blue/Red..0.3-0.4 mm
绿/红.0.4-0.5毫米
黄/绿.0.7至1.0毫米

与下列同步辐射光束线兼容

北美和南美洲
·高级光源，加州伯克利，ALS 4.2.2，ALS 5.0.1，ALS 5.0.2，ALS 5.0.3，ALS 8.2.1，ALS 8.3.1，ALS 11.3.1，ALS 12.2.2，ALS 12.3.2，ALS 12.3.1-APX
*高级光子源，阿贡，伊利诺斯州APS 14-BM-C BioCARS，APS 14-ID-B BioCARS，APS 17-ID IMCA-CAT，APS 19-BM，APS 19-ID，APS 22-BM SER-CAT，APS 22-ID SER-CAT，PS 23-BM-B GA/CA，APS 23-ID-B GA/CA，APS 23-ID-D GA/CA，APS 24-ID-C NE-CAT，APS 24 ID-E NE-CAT，APS 31-ID LR-CAT
·先进微结构和器件中心，巴吞鲁日，路易斯安那州CAMD GCPCC
·康奈尔高能，同步加速器源，伊萨卡，纽约国际象棋A1，国际象棋F1
加拿大光源，加拿大萨斯喀彻温省，CLS 08ID-1，CLS 08B1-1
*巴西同步加速器，光实验室，巴西圣保罗，LNLS D03B-MX1，LNLS W01B-MX2
·斯坦福同步辐射实验室，加州门罗公园，SSRL BL 7-1，SSRL BL 9-1，SSRL BL 9-2，SSRL BL 11-1，SSRL BL 12-2，SSRL BL 14-1

欧洲
·库尔恰托夫同步辐射和纳米技术中心，俄罗斯莫斯科KCSRNT K4.4
·瑞典隆德大学实验室，Max II I 711，Max II I 911-2，Max II I 911-3
*MPG/DESY，德国汉堡，MPGDESY BW6
太阳、圣奥宾、法国太阳PROXIMA 1、太阳PROXIMA 2

亚洲和澳大利亚
·上海同步辐射装置，中国上海SSRF BL17U1
·国家同步辐射研究中心，台湾NSRRC BL13B1，NSRRC BL13C1
·波航加速器实验室，韩国POHANG，PAL 2D，PAL 5C，PAL 7A
·光子工厂，筑波，日本PF BL-5A，PF BL-17A，PF AR-NW12A
超级光子环-8 GeV，日本Spring-8 BL12B2，Spring-8 BL24XU，Spring-8 BL26B1，Spring-8 BL26B2，Spring-8 BL32B2，Spring-8 BL32XU，Spring-8 BL38B1，Spring-8 BL41XU，Spring-8 BL44B2，Spring-8 BL44XU

HR8-200 CrystalCap ALS HT 0.025-0.05 mm CryoLoop – 30 pack
HR8-202 CrystalCap ALS HT 0.05-0.1 mm CryoLoop – 30 pack
HR8-204 CrystalCap ALS HT 0.1-0.2 mm CryoLoop – 30 pack
HR8-206 CrystalCap ALS HT 0.2-0.3 mm CryoLoop – 30 pack
HR8-208 CrystalCap ALS HT 0.3-0.4 mm CryoLoop – 30 pack

RELATED ITEM(S)

Hampton 9 Well Glass Plate & Sandwich Box Setup

发表于由

Hampton 9 Well Glass Plate & Sandwich Box Setup

APPLICATIONS

  • Sitting drop crystallization
  • Seeding
  • Heavy atom soaks
  • Cryo transfers
  • Large drops to produce large crystals for neutron diffraction studies

应用程序
静滴结晶
播种
沉重的原子泡
低温转移
用于中子衍射研究的大液滴产生大晶体

FEATURES 特性

  • Siliconized, 9 well glass plates
  • Concave wells
  • Polystyrene box and support

硅化，9孔玻璃板
凹井
聚苯乙烯盒和支架

DESCRIPTION

When setting up a Sandwich Box, one pipets 25 ml of crystallization reagent or dehydrant into the bottom of the Sandwich Box, then places a support into the box. The 9 well depression plate is placed upon the support. Drops of sample and reagent are pipetted into the depressions and the lid of the Sandwich Box is sealed closed with vacuum grease.

Why Sandwich Boxes? They allow one to use very, very large drops. They are optically superior to plastic plates and glass slides, and offer different equilibration kinetics than other crystallization plates. Each of the Sandwich Box components can be washed and reused so there is little waste with the Sandwich Box Setup. These plates can be used during screening and optimization, but are best suited for final optimization and production of crystals. Since the glass plates can be removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual components so one can customize the system to meet their needs. Square cover slides can be used to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box Setup consists of a square plastic box (4 5/16” x 4 5/16” x 1 1/8”), a polystyrene plate support, and a siliconized, glass plate (4” L x 3 3/8” W (100 mm x 85 mm)) with nine concave depressions (7/8” O.D. x 1/4” D (22 mm x 7 mm)).

Drop sizes up to 800 microliters are possible with the Sandwich Box Setup.

描述
当设置一个三明治盒，一个移液管25毫升的结晶试剂或脱水剂到三明治盒的底部，然后放置一个支撑在盒子里。9孔压板放置在支架上。样品和试剂滴入凹陷处，三明治盒的盖子用真空润滑脂密封。

为什么是三明治盒?它们允许人们使用非常非常大的水滴。它们在光学上优于塑料板和玻璃载玻片，并提供不同于其他晶化板的平衡动力学。每个三明治盒组件都可以清洗和重复使用，因此三明治盒设置几乎没有浪费。这些板可用于筛选和优化，但最适合最终优化和生产晶体。由于玻璃板可以从盒子上拆下来，水晶安装很方便。硅化9孔凹板也适用于重原子浸泡，冷溶液稀释和转移，和播种实验，其中涉及一系列稀释。三明治盒设置可作为一个完整的集合或作为单独的组件，因此可以自定义系统，以满足他们的需求。方形盖滑块可用于密封单个储层，但通常不用于结晶装置。三明治盒设置包括一个方形塑料盒(4 5/16“x 4 5/16“x 1 1/8“)，一个聚苯乙烯板支撑，和一个硅化，玻璃板(4“长x 3 3/8“宽(100毫米x 85毫米))与九个凹凹陷(7/8“od x 1/4“D(22毫米x 7毫米))。

滴大小可达800微升与三明治盒设置。

RELATED ITEM(S)

REFERENCES 参考文献

1. In situ X-ray crystallography. A. McPherson. J. Appl. Cryst. (2000). 33, 397-400.

2. Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum. Kovalevsky, A. Y., Hanson, B. L., Seaver, S., Fisher, S. Z., Mustyakimov, M. & Langan, P. (2011). Acta Cryst. F67, 283-286.

3. Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments. Acta Crystallographica Section D Structural Biology. 75. 10.1107/S2059798319012695.   Andersson, Rebecka & Safari, Cecilia & Båth, Petra & Bosman, Robert & Shilova, Anastasya & Dahl, Peter & Ghosh, Swagatha & Dunge, Andreas & Kjeldsen-Jensen, Rasmus & Nan, Jie & Shoeman, Robert & Kloos, Marco & Doak, R. & Mueller, Uwe & Neutze, Richard & Brändén, Gisela. (2019).

Hampton 50号毛细管Glass Number 50 Capillaries产品详细说明

发表于由

Hampton 50号毛细管Glass Number 50 Capillaries

Glass Number 50 Capillaries

应用：

  X-ray data collection

  Liquid-liquid diffusion crystallization

  Gel acupuncture crystallization

Features

  Glass Number 50 (borosilicate)

  Thin walled – 10 micron

      上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Description 描述
Glass Number 50 (borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.

The Diameter is measured 40 mm from the closed end. The tolerances are as follows

0.1 mm -0.075 / +0.075 mm
0.2 mm -0.050 / +0.050 mm
0.3 mm -0.050 / +0.100 mm
0.4 mm -0.100 / +0.100 mm
0.5 mm -0.100 / +0.100 mm
0.6 mm -0.100 / +0.100 mm
0.7 mm -0.150 / +0.150 mm
0.8 mm -0.150 / +0.150 mm
0.9 mm -0.150 / +0.150 mm
1.0 mm -0.150 / +0.250 mm
1.5 mm -0.250 / +0.250 mm
2.0 mm -0.250 / +0.250 mm

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

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更换CryoLoop环的方法与建议

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更换CryoLoop环的方法与建议

Replacing a Mounted CryoLoop in a CrystalCap 更换 CrystalCap 中已安装的 CryoLoop

Removal 
With one hand, using pliers, grasp the Mounted CryoLoop. Using your the other hand, hold the CrystalCap between your thumb and finger(s). With gentle authority twist the Mounted CryoLoop and Cap in opposing directions and pull the Mounted CryoLoop from the CrystalCap.

Replacement 
Select a Mounted CryoLoop with the desired CryoLoop size. Notice the notches on the Mounted CryoLoop. A standard length Mounted CryoLoop is created by snapping the MicroTube at the second notch from the bottom. Remove the end using your fingers or diagonal cutter. Apply a drop of Super Glue to the top opening of the CrystalCap. Insert the Mounted CryoLoop into this opening and push until the Mounted CryoLoop is stopped by the bottom of the CrystalCap. Epoxy may be used instead of Super Glue for a more permanent fixation of the Mounted CryoLoop to the CrystalCap.

移动
用一只手用钳子抓住已安装的 CryoLoop。 用另一只手将 CrystalCap 夹在拇指和其他手指之间。 以温和的权威将已安装的 CryoLoop 和 Cap 朝相反方向扭转，然后将已安装的 CryoLoop 从 CrystalCap 中拉出。

替代品
选择具有所需 CryoLoop 尺寸的已安装 CryoLoop。 注意已安装的 CryoLoop 上的槽口。 标准长度的已安装 CryoLoop 是通过在从底部开始的第二个凹口处卡入 MicroTube 来创建的。 用手指或斜切刀去除末端。 在 CrystalCap 的顶部开口处涂抹一滴强力胶。 将已安装的 CryoLoop 插入此开口并推动，直到已安装的 CryoLoop 被 CrystalCap 的底部挡住。 可以使用环氧树脂代替强力胶，以便将已安装的 CryoLoop 更永久地固定在 CrystalCap 上。

在普通结晶试剂添加硫酸铵和盐

发表于由

Cryo for Ammonium sulfate and salt based crystallization reagents   用于硫酸铵和盐基结晶试剂的 Cryo

Increase the concentration of ammonium sulfate (cryosalt) (or primary salt acting as a precipitant) in increments of 10% in the presence of 5 to 10% v/v Glycerol.

Evaluate 25-30% w/v glucose, or trehalose or sucrose as cryoprotectants for crystals grown in Ammonium sulfate. Acta Cryst. (2002). D58, 1664-1669. Crystallization of RNA/protein complexes. M. Garber, G. Gongadze, V. Meshcheryakov, O. Nikonov, A. Nikulin, A. Perederina, W. Piendl, A. Serganov and S. Tishchenko.

15 to 30% v/v Ethylene glycol, DMSO, or Glycerol.

Try 1 – 2 M Sodium malonate as a cryoprotectant. Acta Cryst. (2003). D59, 2356-2358. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko.

Place a drop of 75% v/v Paratone-N, 25% v/v Paraffin Oil next to the drop containing the crystal. Remove the crystal from the drop using a CryoLoop and dip the crystal into the oil. Keeping the CryoLoop and crystal immersed, gently move the CryoLoop containing the crystal from the oil into the reagent, back into the oil, back into the reagent, repeating this several times to remove some of the aqueous (reagent) layer from the crystal surface. Cryo cool the crystal.

在 5 至 10% v/v 甘油存在的情况下，以 10% 的增量增加硫酸铵（冰晶盐）（或用作沉淀剂的初级盐）的浓度。

评估 25-30% w/v 葡萄糖、海藻糖或蔗糖作为在硫酸铵中生长的晶体的冷冻保护剂。晶体学报。 （2002 年）。 D58，1664-1669。 RNA/蛋白质复合物的结晶。 M. Garber、G. Gongadze、V. Meshcheryakov、O. Nikonov、A. Nikulin、A. Perederina、W. Piendl、A. Serganov 和 S. Tishchenko。

15 至 30% v/v 乙二醇、DMSO 或甘油。

尝试 1 – 2 M 丙二酸钠作为冷冻保护剂。晶体学报。 （2003 年）。 D59，2356-2358。丙二酸盐：一种用于盐生大分子晶体的多功能冷冻保护剂和稳定溶液。 T. Holyoak、T. D. Fenn、M. A. Wilson、A. G. Moulin、D. Ringe 和 G. A. Petsko。

在含有晶体的液滴旁边放置一滴 75% v/v Paratone-N、25% v/v 石蜡油。使用 CryoLoop 从液滴中取出晶体并将晶体浸入油中。保持 CryoLoop 和晶体浸没，轻轻地将含有晶体的 CryoLoop 从油中移入试剂中，再移回油中，再移回试剂中，重复几次以去除晶体表面的一些水（试剂）层。低温冷却晶体。

Cleaning a CryoLoop   CryoLoop环的清洗方法

发表于由

Cleaning a CryoLoop   CryoLoop环的清洗方法

Soak the CryoLoop in a 0.5% detergent solution in deionized water for 15 minutes. Gently rinse the CryoLoop in deionized water. Allow the CryoLoop to air dry.

If more rigorous cleaning is desired or one does not wish to risk the presence of trace amounts of residual detergent, soak the CryoLoop in 5.0 M Urea formulated in deionized water for 15 minutes. Gently rinse the CryoLoop in deionized water. Repeat three times. Allow the CryoLoop to air dry.

将 CryoLoop 浸泡在 0.5% 去离子水中的清洁剂溶液中 15 分钟。 在去离子水中轻轻冲洗 CryoLoop。 让 CryoLoop 风干。

如果需要更严格的清洁或不想冒残留洗涤剂残留的风险，请将 CryoLoop 浸泡在 5.0 M 去离子水中配制的尿素中 15 分钟。 在去离子水中轻轻冲洗 CryoLoop。 重复三遍。 让 CryoLoop 风干。

冷冻具体问题与结晶方案

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冷冻具体问题与结晶方案

Cryo trouble?

Cryo Trouble? Give the following tips a try next time your crystal is fussy about freezing.

Try X-ray data collection at room temperature.

Evaluate other cryoprotectants. Try CryoPro from Hampton Research, which contains 36 unique cryopreservation reagents.
For more information visit http://hamptonresearch.com/product_detail.aspx?cid=30&sid=189&pid=30

Mixing of different cryoprotectants can have a superior protective effect over single component cryoprotectants of the same total concentration.

冷冻麻烦？ 下次当您的水晶对冷冻很挑剔时，请尝试以下提示。

尝试在室温下收集 X 射线数据。

评估其他冷冻保护剂。 试试 Hampton Research 的 CryoPro，它包含 36 种独特的冷冻保存试剂。
欲了解更多信息，请访问 http://hamptonresearch.com/product_detail.aspx?cid=30&sid=189&pid=30

不同冷冻保护剂的混合比相同总浓度的单组分冷冻保护剂具有更好的保护效果。

Change the rate of cooling.  改变冷却速度。

a) Accelerate the rate of cooling. The fastest cooling rates have been achieved by blowing off the gas layer on liquid nitrogen during plunge cooling. (Hyperquenching for protein cryocrystallography, M. Warkentin et al, J. Appl. Cryst. (2006). 39, 805-811)

b) Slow the rate of cooling. Perhaps better suited to crystals with smaller solvent channels. The key to successful slow cooling of protein crystals is to carefully and completely remove all of the solvent from the surface of the crystal using oil such as Paratone-N, Perfluoropolyether, Mineral, Silicon, NVH or other. Remove ALL of the liquid from the surface of the crystals when using oil. (Slow cooling and temperature-controlled protein crystallography, Warkentin et al 10.1007/s10969-009-9074-y and Slow cooling of protein crystals, Warkentin et al, Volume 42, Part 5, Pages 944-952, October 2009) For more information visit http://hamptonresearch.com/menus.aspx?id=3&sid=138

a) 加快冷却速度。 通过在骤冷期间吹掉液氮上的气体层，可以实现最快的冷却速度。 （蛋白质低温晶体学的超淬灭，M. Warkentin 等人，J. Appl. Cryst. (2006). 39, 805-811）

b) 减慢冷却速度。 也许更适合溶剂通道较小的晶体。 成功缓慢冷却蛋白质晶体的关键是使用 Paratone-N、全氟聚醚、矿物、硅、NVH 或其他油等仔细彻底地去除晶体表面的所有溶剂。 使用油时，除去晶体表面的所有液体。 （缓慢冷却和温度控制的蛋白质晶体学，Warkentin 等人 10.1007/s10969-009-9074-y 和蛋白质晶体的缓慢冷却，Warkentin 等人，第 42 卷，第 5 部分，第 944-952 页，2009 年 10 月） 访问 http://hamptonresearch.com/menus.aspx?id=3&sid=138

Using salt as a crystallization reagent? Many salts are cryosalts, including malonate, formate, citrate, tartrate, acetate, Tacsimate and other organic acids, ammonium sulfate (>3.5 M), lithium sulfate, lithium chloride and other alkylammonium salts. For cryo try increasing your salt concentration by 20%. (Cryosalts: suppression of ice formation in macromolecular crystallography, K. A. Rubinson et al, Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587.

Try the identical cryo procedure again with another crystal.

Vary the time and temperature of the crystal handling steps.

Check the liquid nitrogen level in your dewar and maintain a consistent level, day to day, week to week, month to month, year to year.

Try annealing. (Macromolecular crystal annealing: Techniques and cases studies. Bunick et al, The Rigaku Journal Vol. 15/ number 2/ 1998 and Macromolecular crystal annealing: overcoming increased mosaicity associated with cryocrystallography, Harp et al. (1998). Acta Cryst. D54, 622-8

使用盐作为结晶试剂？很多盐是冰晶盐，包括丙二酸盐、甲酸盐、柠檬酸盐、酒石酸盐、乙酸盐、Tacsimate和其他有机酸、硫酸铵（>3.5 M）、硫酸锂、氯化锂和其他烷基铵盐。对于冷冻，尝试将盐浓度提高 20%。 （冷冻盐：大分子晶体学中冰形成的抑制，K. A. Rubinson 等人，Acta Cryst. (2000). D56, 996-1001 doi:10.1107/S0907444900007587。

用另一个晶体再次尝试相同的冷冻程序。

改变晶体处理步骤的时间和温度。

检查杜瓦瓶中的液氮水平，并每天、每周、每月、每年保持一致的水平。

尝试退火。 （高分子晶体退火：技术和案例研究。Bunick 等人，理学杂志第 15 卷/第 2 期/1998 年和高分子晶体退火：克服与低温晶体学相关的镶嵌性增加，Harp 等人（1998 年）。Acta Cryst. D54， 622-8

Match the osmotic pressure of your cryoprotectant to the osmotic pressure of the reagent producing the crystal. Crystallization reagents with lower salt concentrations require a higher percentage of cryoprotectant for cryo protection than crystallization reagents with higher salt solutions (Cool data: quantity and quality. Elspeth Garman. Acta Cryst. (1999). D55, 1641-1653.). Osmolality tables (Weast, R. C. (1988-1989). Editor. Handbook of Chemistry; Physics, 69th ed. Boca Raton, Florida: CRC Press) can be used to estimate the osmolality of reagents. Another trick is to slowly concentrate a drop of the mother liquor by leaving the drop open to air and allowing the drop to slowly dry down, checking the mother liquor for clear glass freeze every few minutes. If you do not have X-rays to check for clear glass freeze, you can guesstimate by carefully placing the dewar under a dissecting microscope with overhead lighting. Focus on the surface of the liquid nitrogen, and bring the cooled loop into view just above the surface of the liquid nitrogen, where it is cold enough for guesstimate freezing.

使冷冻保护剂的渗透压与产生晶体的试剂的渗透压相匹配。与具有较高盐溶液的结晶试剂相比，具有较低盐浓度的结晶试剂需要更高百分比的冷冻保护剂来进行冷冻保护（酷数据：数量和质量。Elspeth Garman. Acta Cryst. (1999). D55, 1641-1653.）。渗透压表 (West, R. C. (1988-1989). Editor. Handbook of Chemistry; Physics, 69th ed. Boca Raton, Florida: CRC Press) 可用于估计试剂的渗透压。另一个技巧是慢慢浓缩一滴母液，方法是让一滴母液对空气敞开，让液滴慢慢干燥，每隔几分钟检查一次母液是否有透明玻璃结冰。如果您没有 X 射线来检查透明玻璃是否冻结，您可以通过将杜瓦瓶小心地放在带顶灯的解剖显微镜下进行猜测。专注于液氮的表面，将冷却回路置于液氮表面上方的视野中，那里足够冷，可以推测冻结。

Try high pressure cooling. (High-pressure cooling of protein crystals without cryoprotectants, Kim et al, Acta Cryst. (2005). D61, 881–890)

Cryoprotection of delicate crystals – Artem Evdokimov’s humble recipe.
http://www.xtals.org/crystal_cryo.pdf

Two words. Liquid propane.
http://cars9.uchicago.edu/biocars/pages/flashcooling.shtml

Roger S. Rowlett’s Cryoprotectant Strategy
1. Transfer crystals to mother liquor plus 30%v/v glycerol or ethylene glycol (sometimes lower depending on crystallization
reagent).
2. Transfer crystals to mother liquor plus 30%w/v glucose (or try sequential soaks in mother liquor plus 15%w/v and then 30%w/v glucose. Just a few seconds or minutes is usually enough). Glucose or other sugars often work when glycerol or ethylene glycol fails.
3. Try the “no-fail” in situ cryo method, which is a gradual buildup of cryoprotectant. This method is especially appropriate for crystals that cannot tolerate direct transfer to cryoprotectant solution, or for crystals that are especially sensitive to concentration changes in the mother liquor driven by drop evaporation. In our laboratory this method is routinely used with success on otherwise very sensitive crystals. This particular method is adapted for hanging drop crystallization. Ligands can be soaked in at the same time as cryopreservation if included in the cryoprotectant solution at 125% of the final, desired concentration.
a) Prepare a solution of artificial mother liquor plus 30% w/v glucose (40% v/v glycerol or another cryoprotectant can be substituted)
b) Remove a coverslip containing a drop with crystals to be cryoprotected and add 0.25 drop volume (DV) of cryoprotectant solutions (e.g. for a 4 uL drop add 1 uL of cryoprotectant solution). Replace coverslip on well and let stand for 5 minutes. Examine the crystals for cracking and/or dissolution.
c) Repeat the previous step with the following additional cryoprotectant additions: 0.25 DV, 0.50 DV, 1.00 DV, 2.00 DV. After each addition replace the coverslip over the well and let stand for 5 minutes. Examine crystals for cracking and/or dissolution.
d) After the last addition and 5 minute incubation, remove coverlip, fish out crystals with mounting loops and freeze directly in liquid nitrogen. The final glucose concentration will be 24%, sufficient to protect most crystallization solutions from ice formation upon freezing in liquid nitrogen.
This is very gentle, and often works when #1 and #2 does not, but in our hands nearly always increases mosaicity. (But mosaic is better than no diffraction.)
4. Try dragging crystals though Paratone-N to remove surface water from the crystal. This actually nearly always works for us, but is more fussy than #1 or #2, and it is easier to damage crystals during manipulation because of the viscosity of the oil.
I normally plunge protected crystals into liquid nitrogen after mounting.
Ice rings are a good indication of poor cryoprotection, but lack of diffraction could just be your crystals, too. For our latest dataset, we just sorted through 38 crystals until we found a good one. The key, as it turned out, is that all of our beautiful large crystals were apparently difficult to visualize disordered stacks of plates (we didn’t notice this until some fractured during cryo-soaks) whereas some of the small crystals were actually single crystals. We selected a decently diffracting small one and took loooooooooooong frames to get a good data set. (Roger S. Rowlett, Professor, Colgate University Presidential Scholar, Department of Chemistry, Colgate University, NY, USA)

Dig around in the cryoprotectant database for ideas. http://idb.exst.jaxa.jp/db_data/protein/search-e.php

Try 10% glycerol. (Jim Pflugrath)

Try 10 to 20% 2,3-butanediol. It can also reduce mosaicity. Try a quick dip, 30 to 60 seconds.

Fine tune the cryo concentration by screening 2.5% concentration increments, dipping a loop in the cryo buffer, freezing it and collecting X-Ray diffraction data to find the minimum concentration required that produces a clear glass and no ice rings.

Try dragging the crystal through a 1:1 mix of Paratone-N and Mineral oil until most or all the mother liquor from surrounding the crystal has been removed. (David Briggs)

Look back in your screen plates for a different crystal in different reagent, do not pass go, do not collect $200.00 and start over at the top of this tip.

Roger S. Rowlett 的冷冻保护剂策略
1. 将晶体转移到母液中，加入 30%v/v 甘油或乙二醇（有时根据结晶情况降低
试剂）。
2. 将晶体转移到加 30%w/v 葡萄糖的母液中（或尝试在母液中依次浸泡加 15%w/v 和 30%w/v 葡萄糖。通常只需几秒钟或几分钟就足够了）。当甘油或乙二醇失效时，葡萄糖或其他糖通常会起作用。
3. 尝试“不失败”的原位冷冻方法，这是一种逐渐积累的冷冻保护剂。这种方法特别适用于不能直接转移到冷冻保护剂溶液中的晶体，或对液滴蒸发驱动的母液浓度变化特别敏感的晶体。在我们的实验室中，这种方法通常成功地用于其他非常敏感的晶体。这种特殊的方法适用于悬滴结晶。如果在冷冻保护剂溶液中以最终所需浓度的 125% 包含配体，则可以在冷冻保存的同时浸泡配体。
a) 制备人工母液加 30% w/v 葡萄糖的溶液（可以用 40% v/v 甘油或其他冷冻保护剂代替）
b) 取出含有一滴需要冷冻保护的晶体的盖玻片，加入 0.25 滴体积 (DV) 的冷冻保护剂溶液（例如，对于 4 uL 的液滴，加入 1 uL 的冷冻保护剂溶液）。盖好盖玻片，静置 5 分钟。检查晶体是否破裂和/或溶解。
c) 重复上一步，添加以下额外的冷冻保护剂：0.25 DV、0.50 DV、1.00 DV、2.00 DV。每次添加后，更换孔上的盖玻片并静置 5 分钟。检查晶体是否开裂和/或溶解。
d) 最后一次添加和孵育 5 分钟后，取下盖玻片，用固定环捞出晶体并直接在液氮中冷冻。最终的葡萄糖浓度为 24%，足以保护大多数结晶溶液在液氮中冷冻时不会结冰。
这是非常温和的，并且通常在 #1 和 #2 不起作用时起作用，但在我们手中几乎总是会增加镶嵌性。 （但马赛克总比没有衍射要好。）
4. 尝试通过 Paratone-N 拖动晶体以去除晶体中的表面水。这实际上几乎总是对我们有用，但比 #1 或 #2 更挑剔，并且由于油的粘度，在操作过程中更容易损坏晶体。
我通常在安装后将受保护的晶体放入液氮中。
冰环是低温保护不良的一个很好的迹象，但缺乏衍射也可能只是你的晶体。对于我们最新的数据集，我们只是对 38 个晶体进行分类，直到找到一个好的晶体。事实证明，关键是我们所有美丽的大晶体显然很难看到无序的叠层板（直到在低温浸泡过程中有些破裂我们才注意到这一点），而一些小晶体实际上是单晶.我们选择了一个像样的衍射小帧，并用了 looooooooooooong 帧来获得一个好的数据集。 （Roger S. Rowlett，美国纽约州科尔盖特大学化学系科尔盖特大学校长学者教授）

在冷冻保护剂数据库中四处寻找想法。 http://idb.exst.jaxa.jp/db_data/protein/search-e.php

试试 10% 的甘油。 （吉姆·普弗拉格拉斯）

尝试使用 10 至 20% 的 2,3-丁二醇。它还可以减少镶嵌性。尝试快速浸泡 30 到 60 秒。

通过筛选 2.5% 的浓度增量来微调低温浓度，在低温缓冲液中浸一个循环，将其冷冻并收集 X 射线衍射数据，以找到产生透明玻璃和无冰环所需的最低浓度。

尝试将晶体拖过 Paratone-N 和矿物油的 1:1 混合物，直到去除晶体周围的大部分或全部母液。 （大卫布里格斯）

在您的筛板中查看不同试剂中的不同晶体，不要错过，不要收取 200.00 美元，然后从本提示的顶部重新开始。

Mounting thin crystals 安装晶体环方法

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Mounting thin crystals 安装晶体环方法

To mount very thin crystals onto cryoloops, first dip the nylon loop into 0.5% Formvar solution (Fluka # 09819) to form a thin film. The film provides extra support for fragile crystals, and can result in much sharper reflections with just slightly higher background. To clean the loop, dip it in alcohol to dissolve the support. Two notes: (1) the technique works only for crystals grown without organic solvents, and (2) take precautions not to breathe vapor from the formvar solution–the solvent is 1,2 dichloroethane. Formvar is a standard support for electron microscopy grids.

要将非常薄的晶体安装到冷冻环上，首先将尼龙环浸入 0.5% Formvar 溶液 (Fluka # 09819) 中以形成薄膜。 该薄膜为易碎的晶体提供了额外的支撑，并且可以在背景稍高的情况下产生更清晰的反射。 要清洁环，请将其浸入酒精中以溶解支撑物。 两个注意事项：(1) 该技术仅适用于在没有有机溶剂的情况下生长的晶体，以及 (2) 采取预防措施，不要从 formvar 溶液中吸入蒸汽——溶剂是 1,2 二氯乙烷。 Formvar 是电子显微镜网格的标准支持。