标签归档：Hampton蛋白结晶工具

Cryo buffer 冷冻缓冲液

发表于由

Cryo buffer 冷冻缓冲液

We’ve had good success using the well solution directly as the foundation of a cryobuffer in several situations where crystals cannot be grown directly in the presence of cryoprotectant, and where crystals don’t tolerate transfer to artificial mother liquors. The basic protocol is as follows:

1. Remove 100 microliters of the well solution after crystals have grown

2. Split this sample into two 50 microliter aliquots.

3. Add 7.5 mg of dextrose (glucose) to the first aliquot and 15 mg of dextrose to the second. Dissolve by gentle pipeting with a wide-bore tip. This will give two sequential well solutions that now contain 15% and 30% w/v dextrose. If all the dextrose won’t go into the second aliquot, spin hard and remove the supernatant.

4. Transfer the crystal to aliquot 1, equilibrate for 3 minutes, then to aliquot number 2, then freeze. We’ve had a few crystals that routinely crack or blow up when transfered to artificial mother liquor that behave well when transfered to well solution plus glucose. We assume that there is some aspect of the crystal drop (pH, ionic tension, precipitant concentration) that is more effectively reproduced within the well than by separately prepared mother liquors. The nice thing about the protocol above is that you don’t get much of a volume increase when dry dextrose is dissolved in the well solution, so the components in the solution are not diluted.

Finally, if you don’t get a really good freeze, you can try to add about 5% v/v glycerol to aliquot 2 in addition to the 30% w/v dextrose. Reference: Personal communication from Barry Stoddard, Fred Hutchinson Cancer Research Center

冷冻缓冲液
在几种情况下，在存在冷冻保护剂的情况下晶体不能直接生长以及晶体不能转移到人工母液中的情况下，我们直接使用井溶液作为冷冻缓冲液的基础取得了很好的成功。基本协议如下：

1. 晶体生长后取出 100 微升孔溶液

2. 将此样品分成两份 50 微升的等份。

3. 在第一个等分试样中加入 7.5 mg 葡萄糖（葡萄糖），在第二个等分试样中加入 15 mg 葡萄糖。用大口径吸头轻轻吹打溶解。这将给出两个顺序井解决方案，现在包含 15% 和 30% w/v 葡萄糖。如果所有的葡萄糖都不会进入第二个等分试样，请用力旋转并去除上清液。

4. 将晶体转移到 1 号等分试样中，平衡 3 分钟，然后转移到 2 号等分试样中，然后冷冻。我们有一些晶体在转移到人造母液中时会经常破裂或爆炸，当转移到井溶液和葡萄糖中时表现良好。我们假设晶体液滴的某些方面（pH、离子张力、沉淀剂浓度）在井内比单独制备的母液更有效地再现。上述协议的好处是，当干葡萄糖溶解在孔溶液中时，体积不会增加太多，因此溶液中的成分不会被稀释。

最后，如果您没有得到很好的冷冻效果，除了 30% w/v 葡萄糖外，您可以尝试在等分试样 2 中添加约 5% v/v 甘油。参考：来自 Fred Hutchinson 癌症研究中心的 Barry Stoddard 的个人交流

Hampton 酒石酸铵 Ammonium tartrate dibasic

发表于由

Hampton 酒石酸铵 Ammonium tartrate dibasic

Ammonium tartrate dibasic  酒石酸铵

APPLICATIONS  应用

  • Crystallization grade Ammonium tartrate dibasic for formulating screens or for optimization  结晶级酒石酸铵，用于筛选或优化

FEATURES  特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤液
  • 在1+超纯水中配制：在25°C下电阻率为18.2兆，总有机碳<5 ppb，无细菌(<1细菌(CFU/ml))，无热原(<0.03内毒素(EU/ml))，无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

DESCRIPTION

Ammonium tartrate dibasic

Synonym: L-(+)-Tartaric acid diammonium salt or Diammonium tartrate
(NH4)2C4H4O6
C4H12N2O6
Mr 184.15
CAS Number [3164-29-2]
EC Number 221-618-9
Beilstein Registry Number 6120352
RTECS WW8050000
MDL Number MFCD00013073
PubChem Substance ID 24846510
Purity ≥ 99.5%

HR2-679 Measured pH Range: 6.3 – 6.9 at 25°C
HR2-679 Measured Conductivity Range: 118.2 – 134.4 mS/cm at 25°C
HR2-679 Measured Refractive Index Range: 1.39169 – 1.39346 at 20°C

HR2-767 titrated to pH 7.0 using NaOH at 25°C
HR2-767 Measured Conductivity Range: 115.7 – 129.6 mS/cm at 25°C
HR2-767 Measured Refractive Index Range: 1.38057 – 1.38157 at 20°C

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.0005%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%

描述

酒石酸铵

同义词：l-(+)-酒石酸二胺盐或酒石酸二铵
(NH)4)2C4H4O6
C4H12N2O6
Mr 184.15
核证机关编号[3164-29-2]
教统会编号221-618-9
伯尔斯坦登记处编号6120352
RTECS WW8050000
MDL号码MFCD00013073
PubChem物质ID 24846510
纯度≥99.5%

HR2-679测定pH值范围：6.3~6.9℃，25°C
HR2-679在25°C下的电导率范围：118.2~134.4 ms/cm。
HR2-679测量折射率范围：1.39169~1.39346℃，20°C

HR2-767在25℃NaOH滴定至pH7.0
HR2-767测量电导率范围：25°C下115.7~129.6 ms/cm。
HR2-767测量折射率范围：1.38057~1.38157℃，20°C

Al≤0.0005%
AS≤0.00001%
BA≤0.0005%
Bi≤0.0005%
CA≤0.0005%
CD≤0.0005%
CL≤0.005%
CO≤0.0005%
Cr≤0.0005%
Cu≤0.0005%
Fe≤0.0005%
K≤0.005%
Li≤0.0005%
Mg≤0.0005%
Mn≤0.0005%
Mo≤0.0005%
NA≤0.005%
Ni≤0.0005%
PB≤0.0005%
所以4≤0.005%
Sr≤0.0005%
Zn≤0.0005%

HR2-679 2.0 M Ammonium tartrate dibasic 200 mL
HR2-767 1.6 M Ammonium tartrate dibasic pH 7.0 200 mL

REFERENCES  参考文献

1. Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa. J. Y. Ha, J. H. Lee, K. H. Kim, D. J. Kim, H. H. Lee, H.-K. Kim, H.-J. Yoon and S. W. Suh. Acta Cryst. (2006). F62, 139-141.

Hampton盖玻片 Cover Slides（Siliconized Glass & Plastic Slides）

发表于由

Hampton盖玻片 Cover Slides（Siliconized Glass & Plastic Slides）

Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools >

12 mm Siliconized Glass Cover Slides

Images

12 mm硅化玻璃盖板滑片

应用

  • 用于悬挂和坐滴结晶的硅化玻璃盖板滑片
  • 悬滴结晶
  • 坐滴结晶
  • 夹层滴结晶

特征

  • 高纯玻璃
  • 硅化的、疏水性玻璃表面
  • 0.22毫米及0.96毫米玻璃厚度
  • 12毫米玻璃直径
  • 圆
  • 0.22毫米玻璃厚度与紫外线兼容(透射率>85%)

描述

硅化玻璃覆盖幻灯片允许一个液滴悬浮在一个位置，为周围的储油层溶液的蒸汽扩散提供了近乎佳的条件。

这些硅化玻璃覆盖幻灯片提供了一个一致的，高质量的完成结晶实验。疏水性表面会产生一滴“很好”的水滴，在玻璃上不会变平。硅化表面防止晶体粘附并沉淀到玻璃表面。使用陶氏康宁真空润滑脂(HR3-510)或陶氏康宁7释放化合物(HR3-508)密封滑动板。可在12，18或22毫米直径圆和22毫米直径方格。有0.22毫米或0.96毫米玻璃厚度。0.96毫米厚的幻灯片在晶化处理过程中几乎是不可破碎的。

临界表面张力未处理玻璃78 dynes/cm硅化玻璃31 dynes/cm表面电阻率未处理玻璃1×1012欧姆硅化玻璃1.2×1013欧姆摩擦系数静态，玻璃玻片上未经处理的0.9-1.0硅化0.2-0.3蛋白吸附(100小时)未处理玻璃0.13mg/mm2硅化玻璃0.01-0.02 mg/mm2

以下幻灯片为紫外线可传输(>85%)：
HR3-277和HR3-279-12毫米x 0.22毫米硅化圆盖幻灯片
HR3-231和HR3-233-22毫米x 0.22毫米硅化圆盖幻灯片
HR3-239和HR3-241-18毫米x 0.22毫米硅化圆盖幻灯片
HR3-215和HR3-217-22毫米x 0.22毫米硅化方盖幻灯片

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

FEATURES

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 12 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

DESCRIPTION

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

The following slides are UV transmissible (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

CAT NO NAME DESCRIPTION
HR3-278T Siliconized circle cover slides for Tecan robot 0.5 oz pack (~240 slides)
HR3-277 12 mm x 0.22 mm Siliconized circle cover slides 0.5 ounce pack (~240 slides)
HR3-279 12 mm x 0.22 mm Siliconized circle cover slides 5.0 ounce case (~2,400 slides)
HR8-088 12mmx0.96mmThick Siliconized circle cover slides 1.0 ounce pack (~106 slides)
HR8-090 12mmx0.96mm Thick Siliconized circle cover slides 10.0 ounce case (~1,060 slides)
18 mm Siliconized Glass Cover Slides

Images

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization
CAT NO NAME DESCRIPTION
HR3-239 18 mm x 0.22 mm Siliconized circle cover slides 0.5 ounce pack (~100 slides)
HR3-241 18 mm x 0.22 mm Siliconized circle cover slides 5.0 ounce case (~1,000 slides)
HR3-515 18mmx0.96mm Thick Siliconized circle cover slides 1 oz pack (~45 slides)
HR3-517 18mmx0.96mm Thick Siliconized circle cover slides 10 oz case (~450 slides)
22 mm Siliconized Glass Cover Slides

Images

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization.
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization
CAT NO NAME DESCRIPTION
HR3-231 22 mm x 0.22 mm Siliconized circle cover slides 1.0 ounce pack (~120 slides)
HR3-233 22 mm x 0.22 mm Siliconized circle cover slides 10.0 ounce case (~1,200 slides)
HR3-247 22 mm x 0.96 mm Thick Siliconized circle cover slides 3 ounce pack (~75 slides)
HR3-249 22mmx 0.96mm Thick Siliconized circle cover slides 30.0 ounce case (~750 slides)
HR3-215 22 mm x 0.22 mm Siliconized square cover slides 1.0 ounce pack (~100 slides)
HR3-217 22 mm x 0.22 mm Siliconized square cover slides 10.0 ounce case (~1,000 slides)
HR3-223 22mmx0.96mm Thick Siliconized square cover slides 4.0 ounce pack (~75 slides)
HR3-225 22mmx0.96mm Thick Siliconized square cover slides 40.0 ounce case (~750 slides)

Images

Glass Cover Slide Gizmo Dispenser

Images

Siliconized Glass Coverslips (Greiner)

Images

OptiClear Plastic Cover Slides

Images

PEN-VAC Kit

Images

Cover Slide Vacuum Gadget

Hampton HR3-508 Dow Corning 7 Release Compound Grease

发表于由

Hampton HR3-508 Dow Corning 7 Release Compound Grease

APPLICATIONS

  • Grease sealant for hanging, sitting, and sandwich drop vapor diffusion set ups.

应用
用于悬挂式、坐式和夹层式液滴蒸汽扩散装置的润滑脂密封剂。

DESCRIPTION

The Dow Corning® 7 Release Compound Grease sealant is similar to vacuum grease but is less viscous. Being less viscous, it is easier to push through a syringe when applying the sealant manually. Squeeze the grease into a 10 cc syringe fitted with a 200 µl pipet tip trimmed to desired diameter and you are ready to grease.

描述
道康宁® 7 离型复合润滑脂密封剂类似于真空润滑脂，但粘性较低。 由于粘性较小，手动涂抹密封剂时更容易推动注射器。 将润滑脂挤入装有 200 µl 移液管尖端的 10 cc 注射器中，并修剪到所需的直径，您就可以润滑了。

HR3-508 Dow Corning 7 Release Compound Grease 150 g tube

Hampton 96孔蛋白结晶板Crystallization Plate (Swissci)

发表于由

Hampton 96孔蛋白结晶板Crystallization Plate (Swissci)

Crystallization Plates, Hardware & Accessories | 96 Well Crystallization Plates

Images

MRC Under Oil 96 Well

Images

MRC 2 Well UVP

Images

MRC 2 Well UVXPO
3 Well Midi Crystallization Plate

Images

3 Well Low Profile Crystallization Plate
LCP Lipidic Cubic Phase Screening
Diaplate

Hampton 96 孔蛋白结晶板Crystallization Plate (Swissci)

Diaplate
96 well 3.2 µl capacity dialysis plate

CrystalEX (Corning)
Sitting drop crystallization

CrystalEX Second Generation (Corning)
Sitting drop crystallization

CrystalQuick 96 Well Sitting Drop Plate (Greiner)
Sitting drop crystallization

Intelli-Plate 96 (Art Robbins Instruments)
Sitting drop crystallization

MRC 2 Well Crystallization Plate (Swissci) UVXPO
Sitting drop crystallization UVXPO

MRC 2 Well Crystallization Plate (Swissci) UVP
Sitting drop crystallization UVP

3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

FRAP Sandwich Set
FRAP Sandwich Set

LCP Sandwich Set
Crystallization screening of membrane proteins in lipidic mesophase as well as bicelle method and batch method

LCP Lipidic Cubic Phase Screening plate (Swissci)
Lipidic cubic phase crystallization

MRC Under Oil 96 Well Crystallization Plate (Swissci)
Microbatch crystallization

Imp@ct Plate without reservoir (Greiner)
Microbatch crystallization

MASTERBLOCK® 96 Deep Well Plate (Greiner)
Reagent storage

Microplate Lid
Cover for 96 well HT Screens and Masterblock

Hampton 96 孔蛋白结晶板Crystallization Plate (Swissci)

3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

CAT NO NAME DESCRIPTION
HR3-123 3 Well Midi Crystallization Plate (Swissci) 10 plate case
HR3-125 3 Well Midi Crystallization Plate (Swissci) 40 plate case
HR3-205 3 Well Low Profile Crystallization Plate (Swissci) 10 plate case
HR3-206 3 Well Low Profile Crystallization Plate (Swissci) 40 plate case

MRC 2 Well Crystallization Plate (Swissci) UVP

Sitting drop crystallization UVP

CAT NO NAME DESCRIPTION
HR3-082 MRC 2 Well Crystallization Plate 10 plate case
HR3-083 MRC 2 Well Crystallization Plate 40 plate case

MRC 2 Well Crystallization Plate (Swissci) UVXPO
Sitting drop crystallization UVXPO

CAT NO NAME DESCRIPTION
HR3-106 MRC 2 Well Crystallization Plate in UVXPO 10 plate case
HR3-107 MRC 2 Well Crystallization Plate in UVXPO 40 plate case

MRC Under Oil 96 Well Crystallization Plate (Swissci)
Microbatch crystallization

Applications
Microbatch crystallization

Features
Easy crystal retrieval
Easy viewing
UV imaging compatible
Drop Volume 100 nanoliter to 4 microliter
Oil Volume 20 µl
SBS Standard
Ultra low binding polymer, no static

Description 
The MRC Under Oil 96 Well Crystallization Plate is designed for microbatch crystallization.

The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, United Kingdom) in collaboration with Dr. Jan Löwe and colleagues. It is a result of many years of experience in successful robotic high-throughput crystallization and combines many of the features not earlier available to the crystallographer.

The new MRC Under Oil 96 Well Crystallization Plate is designed for microbatch crystallization using paraffin, silicon oil, or a mixture of the two. Following the initial experiment, evaporation of the drop through the oil allows for second crystallization stage, enabling further crystal growth as a consequence of concentration. This is different from other experiments in that the conditions are then extreme in nature and permit new conditions to arise.

The new MRC Under Oil 96 Well Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from an optically superior polymer and with a new design of the wells, the plate allows easy crystal viewing and retrieval.

The advantages of the new MRC Under Oil 96 Well Crystallization Plate.

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.

Easy Viewing
The wells are wide conical and have a polished surface on both sides important for perfect illumination. The micro numbering ensures that you will never get lost again (visible by microscope). The optically superior polymer is even UV transmissible and may be used to differentiate between salt and crystals.

Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape for the initial experiments of microbatch. No central bending occurs in this very robust structure.

Recommended Volumes
Typical volumes validated for these plates are 20 µl of oil with a shot through sample delivery of 100 nanoliter to 4 microliter.

SBS Standard
The plates are designed to the 96 well SBS standard for all common holders and external numbering (A – H, 1 – 12) with corner location that make the plate easy to use in a robotic sampler. The plate can also be centrifuged for better results. The unique MRC Under Oil 96 well Crystallization plate offers a new way of microbatch crystallography. The 96 wells are optically perfect, designed to observe crystals under a microscope.

Unique Polymer
The proprietary polymer is optically perfect – ultra low binding and guaranteed to have central drop location in the well. There are no static effects and thus micro-droplet jumping is avoided.

CAT NO NAME DESCRIPTION
HR3-102 MRC Under Oil Crystallization Plate 10 plate case
HR3-104 MRC Under Oil Crystallization Plate 40 plate case

Universal V1-Puck (Uni-Puck) 使用说明

发表于由

Universal V1-Puck (Uni-Puck)

The Universal V1-Puck (Unipuck) is a sample pin storage and shipping container compatible with many automated sample mounting systems. The V1-puck holds 16 MiTeGen sample bases/pins. It consists of two parts shown in the image: 1) the sample enclosure (left) and 2) the sample base piece (right).

Each part of the V1-puck has a unique serial number for identification. Custom serial numbers are available if requested at time of order.

Universal V1-Puck (Unipuck) 是一种样品针存储和运输容器，与许多自动样品安装系统兼容。 V1-puck 可容纳 16 个 MiTeGen 样品底座/引脚。 它由图像中显示的两部分组成：1) 样品外壳（左）和 2）样品底座（右）。

V1-puck 的每个部件都有一个唯一的序列号用于识别。 如果在订购时要求，可提供自定义序列号。

Product Information  其他相关产品：

The tools for handling Universal V1-pucks are available individually or in our convenient Uni-puck Starter Kits.  Looking for a set of pucks, or outfitting a facility? You can see our bundle options available here: Bulk pricing options

  • Uni-Puck Multi Color Puck Option

    Get your Uni-pucks set in a series of unique colors. Help identify which puck you are working with. Available…

  • Universal Puck (Uni-Puck) Starter Kits

    To get you up–and–running to the beamline All the components you
    The Universal Puck Project

    A collaboration to develop the uni-puck, a new standard sample pin storage and shipping container.

    The uni-puck has been designed to be compatible with all robotic mounting systems currently in use at synchrotrons in the United States and many systems used abroad, requiring minimal changes to the robotic hardware of each system.

    Introduction

    The availability of robotic sample mounting systems continues to increase at synchrotron sources. (See smb.slac.stanford.edu/robosyncfor a list of available devices). Although the majority of these systems are compatible with Hampton-style magnetic cryo-pins, many types of containers are used for sample pin transportation and storage.

    Currently at synchrotron stations in the United States there are three main sample storage containers used: the ALS puck, the SSRL cassette, and the MSC magazine. The costs to purchase these containers and associated cryo-tools and the effort required to master their use has become a significant impediment to groups seeking to take advantage of more than one of these facilities.

    In an effort to minimize this burden, a collaboration was formed between the ALS, APS SBC-CAT, and SSRL staff. This group first proposed construction of a sorting system that would transfer sample pins between the most commonly used containers. However, it was observed that it could be possible to develop a sample storage container that would be compatible with all the sample mounting systems currently in use in the United States and many of the systems used abroad. Therefore, during a meeting at the 2004 ACA conference in Chicago the group decided to develop a universal container. We have received a lot of positive feedback in response to this effort. In particular, many have joined this collaboration including developers at the NSLS, APS, and CHESS and of commercial systems. These developers were asked to test the V1 uni-puck with their automated mounting systems and provide feedback on the design with the goal of cross compatibility and reliability. Through the collaborative efforts of all involved, a first version of the universal container, the V1 uni-puck has been completed. The V1 uni-puck was released for use at the ALS sector 5.0 beam lines and at the SSRL macromolecular crystallography beam lines with test users. Once groups have more experience using the V1 uni-puck, a second version (V2) may be released if necessary for improved performance.

    Design

    The outside form factor of the new universal container (uni-puck), resembles a standard ALS puck and for this reason will be compatible with the standard ALS cryo-tools and fit inside the ALS shipping dewar canister. Unlike the ALS puck, the pins are held inside the container using rare earth magnets which are more durable and more strongly and reliably hold the pins in place. Magnets are used in both the enclosure piece and base piece of the uni-puck. The uni-puck enclosure resembles an SSRL cassette as it contains ports that are nearly identical to those in an SSRL cassette. The uni-puck also provides the same clearance around the sides and bottom of the sample pin as an MSC magazine and the base piece shares a similar hole and slot for alignment inside the dispensing dewar.

    The universal puck consists of two parts: 1) the base piece and 2) the sample enclosure. Some key differences between the original ALS puck and universal puck are illustrated in the figures below.

    Both the universal puck enclosure and the base contain magnets and the assembly will hold together by magnetic force. Therefore, the side clips used on the ALS puck for this purpose are not necessary. However to enable the universal puck parts to fit interchangeably with ALS puck parts, clearance and mounting holes for these clips are provided. Interchangeability of the universal puck base and enclosure with the ALS puck parts enable rapid transfer of samples between these two containers.
    Compatibility of Specific Robotic Systems

    The ALS Robot (Berkeley Automated Mounting system)

    The uni-puck base piece is used inside the ALS dispensing dewar. This universal base will work in the ALS robotic systems interchangeably with the original ALS puck base. Because the sample pins are held in the same position, minimal hardware modifications or reprogramming is required.

    Because the outside form factor is similar, the V1 uni-puck is compatible with the standard ALS cryo-tools and shipping dewar canister. Additionally, the uni-puck base and enclosure pieces fit on the original ALS puck enabling rapid transfer between the two types of containers.

    Members of the ALS beam line development group are taking part in the universal puck project and will ensure compatibility and reliability with their automounter.

     

    The Stanford Automated Mounting System (SAM)

    The enclosure piece of the universal puck is used inside the SAM dispensing dewar. This piece contains magnetic cavities nearly identical to those in a SSRL cassette. To adapt the enclosure piece to the standard SAM setup an adaptor cassette has been developed. Four universal pucks may be inserted into one adaptor cassette. The adaptor is then inserted inside the robot dispensing dewar using the cassette transport handle (see below).

    The original SSRL cassettes will continue to work with the SAM system. Within the SSRL dispensing dewar the 3 cassette locations are each capable of interchangeably holding an SSRL cassette or an adaptor cassette containing 4 universal pucks.

    Once the universal container project and adaptor cassette development is complete, SSRL users will have the option to use cassettes or uni-pucks and the SAM system will be programmed to sort samples between these two options.
    APS SBC-CAT Automatic Mounting System

    Currently the SBC-CAT system works with MSC magazines. To work with the uni-puck, the system will be reprogrammed and a new dewar base plate will be developed that can accommodate both MSC magazines and universal puck bases. Users will then have the option to use either container.

    For added reliability when picking up a sample pin, the SBC-CAT robot grippers surround the bottom edge of the pin trapping it in place (rather than gripping the side of the pin and relying on friction to hold it). To accommodate this gripper, the uni-puck base provides additional clearance around the bottom and side of the pin base. This clearance is equal to that provided by the MSC magazine.

    Members of the APS SBC-CAT development group have been instrumental in the universal puck project and will ensure compatibility and reliability with their automounter.

    Commercially Available Systems

    This universal container concept has received positive feedback from the commercial manufacturers of robotic sample mounting systems (MSC, Bruker, and Mar). Moreover, MSC and Bruker representatives feel that it would be rather straight-forward for them to offer an alternative dewar base plate and programming to accommodate the proposed universal puck design.

    Fabrication of an alternative enclosure, that would contain traditional cryo-vials and mate with the universal puck base, will make it easy to transfer samples from vials into pucks and enable compatibility with even more robotic mounting systems.

Taylor Wharton CX100 液氮罐（带运输箱）

发表于由

Taylor Wharton CX100 液氮罐（带运输箱）

Taylor Wharton CX100 Dry Shipper, with Canister & Shipping Case

Cryo Express (CX). Cryo Express (CX) “dry shippers” are designed to safely transport a variety of materials at cryogenic temperatures. The unique adsorbent material prevents a liquid spill if the unit is tipped over. Storage temperature inside the shipping cavity remains at approximately -190˚C until the liquid nitrogen evaporates from the absorbent material.

冷冻快递 (CX)。 Cryo Express (CX)“干货运输机”旨在在低温下安全运输各种材料。 如果装置翻倒，独特的吸附材料可防止液体溢出。 运输腔内的储存温度保持在大约 -190˚C，直到液氮从吸收材料中蒸发。

SKU: CP-111-049

Taylor Wharton CX100 液氮罐

发表于由

Taylor Wharton CX100 液氮罐

液氮罐 CX100简单介绍：

泰来华顿国际是一家传统型生产制造企业 主要从事于广泛的气体应用领域 被称为其行业内的风向标。美国哈斯科集团旗下的泰来华顿气体设备有限公司创建于1776年，是世界上早和大的一家生产低温设备的公司。.泰来华顿国际出售的产品显著的特点就是能够满足全世界对于新产品 新技术 新应用日益不断的需求。泰来华顿国际旗下拥有4个互为补充的生产制造与售后服务为一体的子公司 形成了世界上在气体应用领域内集技术 服务 生产制造于一身的体系 其中包括各种压力容器，低温容器比如液氮罐CX100和高压气体阀门。 泰来华顿国际在全球4大洲6个设有20个包括生产 销售 仓储 服务在内的厂房 其出售的产品遍布世界80多个. 优势产品：泰来华顿液氮罐，泰来华顿缩气体储存容器 泰来华顿低温液体储存容器 泰来华顿生物运输容器 泰来华顿生物低温储存容器 泰来华顿液化天然气储存容器 泰来华顿车用燃料储存容器泰来华顿压缩天然气储存容器 泰来华顿及加工半导体芯片储存容器。

Worthington Industries 沃辛顿工业，是全球知名的平轧钢和压力容器制造商，其深低温存储设备源自Taylor-Wharton泰莱华顿。

Taylor-Wharton泰莱华顿是美国历史最悠久的，仍在运营的金属制品公司，其历史可以追溯到1742年。早期的产品包括有给乔治华盛顿军队生产的炮弹; 美国登月宇航员背负设备中所用的罐体及管道。其 CryoScience深冷科技部门早在1957就开始生产液氮生物深低温容器。经过一系列的技术发展和时间的检验，现在产品系列涵盖深低温气相/液相液氮存储罐，干式液氮运输罐，液氮杜瓦补充罐等所有行业需求类别。

2015年底,  沃辛顿工业与泰莱华顿CryoScience深冷科技部门强强联合，完成了优势重组。现在历史悠久，科技先进和资金雄厚的Worthington Industries – Taylor Wharton Cryoscience  WI-TWC邀您一起加入生命科学探索之旅。

液氮罐CX100详细参数：
CX系列液氮罐、液氮容器产品用于运送低温保护下的精子，胚胎组织等，其容器内放有干燥剂及吸附材料，因此在容器倾倒时具有液态氮不会流出的特点，该容器适用于空运时运送标本，也可在出差时携带，具有体积小，运输方便的特点，此外该容器亦采用一字型焊接，具有蒸发率低、外观优美，弧线结构等特点。液氮罐CX100的CXR系列产品，可以更换内胆。

型号：CX100
静态储存天数：24天
工作天数：17天
蒸发率：0.18升/天
液氮容量：4.4升
空重：5.3Kg
满重：8.9Kg
颈部直径：71mm
高度：467mm
直径：234mm
提筒数量：1
2.0ml冻存管容量：1820支
0.5ml麦管：490支

型号：CXR100
静态储存天数：16天
工作天数：11天
蒸发率：0.20升/天
液氮容量：3.3升
空重：5.3Kg
满重：8.9Kg

液氮罐(含提桶一只)

货号：CX100B-11M

CX系列适用于空运携带标本、血袋及其它须低温存放样本。

CX系列参数

品牌：Taylor-Wharton 型号：CX系列 容积：  
重量：5.3-13.6（kg） 适用物料：液氮  
型号 CX100 CXR100 CXR500
静态保存天数 (1) 22 16 11
工作天数 (2) 17 11 7
蒸发率(1)    公升/天 0.19 0.23 0.64
液氮容量      公升 4.1 3.7 7
空罐重量 磅 11.7

5.3

 11.7

5.3

 30

13.6
公斤
满罐重量  (3) 磅 19

8.6

 18.3

8.3

 42.5

19.3
公斤
颈部直径 英寸 2.78

71

 3.58

91

 8.5

216
毫米
总高度 英寸 18.4

467

 19.4

493

 26.9

683
毫米
总直径 英寸 9.2

234

 9.2

234

 15.5

391
毫米
 小桶数量 1 1 N/A
小桶尺寸

 

 英寸 2.64*11.0

67*2.79

 2.64*11.0

67*2.79

 N/A

N/A
毫米
1.2 升 & 2.0 升小瓶数量(5/铁罐) (4) 85 85 500
1.2 升 & 2.0 升小瓶数量(6/铁罐) (4) 102 102 500
1/2 cc 径管数量

(10/铁罐)

 280 280 N/A
1/2 cc 径管数量

散装(2 层)

 980 980 N/A
装运箱 CX10-8C00 C10-8C00 CP19-8C00
填充纸板箱 3701-9Z77 N/A N/A
5 层架子 N/A N/A RS30-9C44
100格聚乙烯盒 N/A N/A R24K-9C44

Hampton 硅化玻璃盖玻片Siliconized Glass Coverslips (Greiner)

发表于由

Hampton 硅化玻璃盖玻片Siliconized Glass Coverslips (Greiner)

APPLICATIONS

  • Hanging or sitting drop vapor diffusion crystallization

FEATURES

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.19-0.22mm glass thickness
  • 18 & 22 mm glass diameter
  • Circle glass cover slip
  • UV imaging compatible (>85% transmission)

DESCRIPTION

Siliconized glass coverslips allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use vacuum grease or oil to seal cover slide to plate. Available in 18 or 22 mm diameter circles.

HR3-254 is Greiner Bio-One 503870 siliconized glass coverslip, round, 22mm diameter, 0.19-0.22mm thick. Non-sterile. 100 pieces per inner pack. Ten inner packs, totaling 1,000 pieces per packing unit. Fit VDX Plate.

HR3-255 is Greiner Bio-One 501870 siliconized glass coverslip, round, 18mm diameter, 0.19-0.22mm thick. Non-sterile. 100 pieces per inner pack. Ten inner packs, totaling 1,000 pieces per packing unit. Fits VDXm Plate and ComboPlate (Greiner 662050 and 662150).

应用

  • 悬吊或坐滴蒸汽扩散结晶

特征

  • 高纯玻璃
  • 硅化的、疏水性玻璃表面
  • 0.19-0.22mm玻璃厚度
  • 18&22毫米玻璃直径
  • 圆玻璃盖滑
  • 紫外成像兼容(透射率>85%)

描述

硅化玻璃圆柱体允许一个液滴悬浮在一个位置，这为周围的储油层溶液的蒸汽扩散提供了近乎最佳的条件。这些硅化玻璃覆盖幻灯片提供了一个一致的，高质量的完成结晶实验。疏水性表面会产生一滴“很好”的水滴，在玻璃上不会变平。硅化表面防止晶体粘附并沉淀到玻璃表面。用真空润滑脂或油密封盖子、滑动板。可分为18或22毫米直径圆。

HR3-254是Greiner Bio-one 503870硅化玻璃圆孔，直径22毫米，厚0.19-0.22mm.不不育。每个内包装100件。10个内包装，每包装1,000件。安装VDX板。

HR3-255是Greiner Bio-one 501870硅化玻璃圆孔，直径18毫米，厚0.19-0.22mm.不不育。每个内包装100件。10个内包装，每包装1,000件。适合VDXM板和组合板(Greiner 662050和662150)。

HR3-254 22mm Siliconized Circle Coverslips Pack (10×100 = 1,000)
HR3-255 18mm Siliconized Circle Coverslips Pack (10×100 = 1,000)

Hampton 结晶loop环底座 CrystalCap ALS

发表于由

Hampton 结晶loop环底座 CrystalCap ALS

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers

DESCRIPTION

The CrystalCap ALS is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™.

CrystalCap ALS (HR4-779) is supplied without a vial (HR4-904).

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS is compatible with ALS style grippers, auto mounters and sample handlers.

ALS style sample mounters accept the CrystalCap ALS.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A • Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

应用

  • 冰晶术

特征

  • AlS型结晶架
  • 结晶帽ALS磁性附着在结晶帽Vial磁力仪基座
  • 可与ALS风格的夹持器、自动装配器和示例处理程序兼容的自由锥形形状

描述

结晶帽ALS是一个磁性样品安装(也称为盖子，引脚或测角器底座)，为接受ALS型样品安装的晶晶系统设计。水晶帽ALS附着在磁性水晶帽的气门和磁测角仪的基座上。水晶帽的顶端接受安装的冷冻环™。

晶体帽ALS(HR4-779)是在没有小瓶(HR4-904).

水晶瓶盖是一个1.8毫升的冰瓶，有两个小通风口。将环形磁铁模压到瓶的开口端，以便当盖被放置在瓶中时，环磁铁在低温储存期间将盖保持在瓶上。HR4-904水晶帽瓶底部没有磁铁。

水晶帽ALS的无棱镜锥形形状与ALS风格的夹持器、自动装配器和样品处理程序兼容。

ALS风格的样品贴片机接受水晶帽ALS。

与下列同步辐射光束线兼容

北美和南美洲
·高级光源，加州伯克利，ALS 4.2.2，ALS 5.0.1，ALS 5.0.2，ALS 5.0.3，ALS 8.2.1，ALS 8.3.1，ALS 11.3.1，ALS 12.2.2，ALS 12.3.2，ALS 12.3.1-APX
*高级光子源，阿贡，伊利诺斯州APS 14-BM-C BioCARS，APS 14-ID-B BioCARS，APS 17-ID IMCA-CAT，APS 19-BM，APS 19-ID，APS 22-BM SER-CAT，APS 22-ID SER-CAT，PS 23-BM-B GA/CA，APS 23-ID-B GA/CA，APS 23-ID-D GA/CA，APS 24-ID-C NE-CAT，APS 24 ID-E NE-CAT，APS 31-ID LR-CAT
·先进微结构和器件中心，巴吞鲁日，路易斯安那州CAMD GCPCC
·康奈尔高能，同步加速器源，伊萨卡，纽约国际象棋A1，国际象棋F1
加拿大光源，加拿大萨斯喀彻温省，CLS 08ID-1，CLS 08B1-1
*巴西同步加速器，光实验室，巴西圣保罗，LNLS D03B-MX1，LNLS W01B-MX2
·斯坦福同步辐射实验室，加州门罗公园，SSRL BL 7-1，SSRL BL 9-1，SSRL BL 9-2，SSRL BL 11-1，SSRL BL 12-2，SSRL BL 14-1

欧洲
·库尔恰托夫同步辐射和纳米技术中心，俄罗斯莫斯科KCSRNT K4.4
·瑞典隆德大学实验室，Max II I 711，Max II I 911-2，Max II I 911-3
*MPG/DESY，德国汉堡，MPGDESY BW6
太阳、圣奥宾、法国太阳PROXIMA 1、太阳PROXIMA 2

亚洲和澳大利亚
·上海同步辐射装置，中国上海SSRF BL17U1
·国家同步辐射研究中心，台湾NSRRC BL13B1，NSRRC BL13C1
·波航加速器实验室，韩国POHANG，PAL 2D，PAL 5C，PAL 7A

HR4-779 CrystalCap ALS without Vial – 60 pack

RELATED ITEM(S)

Sample Containers:

A: SSRL cassette

 

B: ACTOR magazine

 

C: Cryo-vial basket or EMBL/ESRF cryo-vial basket

 

D: ALS puck

E: Uni-puck

F: miniSPINE puck

Sample Pin Bases:

A: Hampton magnetic

 

B: Hampton copper magnetic

 

C: ALS-style

 

C: ALS-style

 

D: Mitegen B3S

E: SPINE

F: miniSPINE