Benzylkonium chloride-膜蛋白和可溶性蛋白用阳离子表面活性剂
Try benzylkonium chloride (Fluka 12060). This cationic surface active agent has been reported to be useful as a crystallization additive with membrane proteins and may be useful for soluble proteins. We’ve been using it in the drop between 1 and 3% w/v in water. Try a 10% w/v stock solution in water and dilute into the drop to 1-3%.
苄基氯铵
试试苄基氯铵 (Fluka 12060)。 据报道,这种阳离子表面活性剂可用作膜蛋白的结晶添加剂,并可用于可溶性蛋白。 我们一直在水中使用 1% 到 3% w/v 的浓度。 尝试在水中使用 10% w/v 的储备溶液,然后将其稀释至 1-3%。