Hampton research HR2-102 StockOptions Hepes

发表于由

Hampton research HR2-102 StockOptions Hepes

StockOptions Hepes Buffer Kit

APPLICATIONS

  • Focused, titrated buffer stocks for crystal screening, optimization, & production

FEATURES

  • pH 6.8 to 8.2 (15 reagents)
  • 0.1 pH unit increments
  • 1.0 M concentrated stocks

DESCRIPTION

StockOptions Buffer kits are reagent toolboxes for the macromolecular crystal grower. Stock-Options reagent kits offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in StockOptions kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.

Each 1.0 M Hepes solution has been titrated using NaOH.
HR2-102 contains fifteen unique 10 milliliter volumes of 1.0 M Hepes buffer solution titrated to the indicated pH using NaOH.

HEPES
Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry 883043
Merck 14,4654
RTECS TL6809000
Purity > 99.0%
pKa 7.5

Useful pH range of HEPES buffer 6.8-8.2

HR2-102 StockOptions™ Hepes pH 6.8 to 8.2 in 0.1 pH unit increments

RELATED ITEM(S)

REFERENCES

1. The expression, purification and crystallization of the epsilon subunit of the F1 portion of the ATPase of Escherichia coli. Codd, R; Cox, GB; Guss, JM; Solomon, RG; Webb, D. J Mol Biol 228, 306- 309, 1992.

2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.

3. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens