Looking for yet another additive to try during optimization? Try glycerol. Typical concentrations in the drop can range from 3 to 15%. Glycerol may limit non-specific aggregation and could be useful if you plan to use cryo techniques during data collection.

When adding glycerol to the vapor diffusion crystallization experiment be sure to have a higher glycerol concentration in the reservoir than the drop otherwise you may find that your drops will get biggger and not smaller. Reference: Vera,L., Czarny, B., Georgiadis, D., Dive, V., Stura, E.A. (2011) Practical Use of Glycerol in Protein Crystallization. Cryst. Growth & Des. 11 :2755–2762

甘油
正在寻找另一种在优化过程中尝试的添加剂？ 试试甘油。 液滴中的典型浓度范围为 3% 至 15%。 甘油可能会限制非特异性聚合，如果您计划在数据收集期间使用冷冻技术，它可能会很有用。

在蒸汽扩散结晶实验中添加甘油时，请确保容器中的甘油浓度高于液滴，否则您可能会发现液滴会变得更大而不是更小。 参考： Vera,L.、Czarny, B.、Georgiadis, D.、Dive, V.、Stura, E.A. (2011) 甘油在蛋白质结晶中的实际应用。 水晶。 成长与发展。 11:2755–2762