Got ice rings and want to get rid of them?

While the crystal is in the beam, carefully try the following.

Try annealing the crystal by blocking the stream for a few seconds, allowing the crystal to thaw, then unblock the stream to cool the crystal again. (Reference: New techniques in macromolecular cryocrystallography: macromolecular crystal annealing and cryogenic helium, B. Leif Hansona, Constance A. Schallb and Gerard J. Bunick, Journal of Structural Biology
Volume 142, Issue 1, April 2003, Pages 77-87)

Try washing the crystal with liquid nitrogen. Using a pipette, carefully aspirate liquid nitrogen into the pipette, then dispense several drops or even a gentle stream over the crystal and mount. A liquid nitrogen wash can also be used to remove creeping ice from caps, pins and loops.

有冰环并想摆脱它们？

当晶体在光束中时，请仔细尝试以下操作。

尝试通过阻塞流几秒钟来使晶体退火，让晶体解冻，然后解除阻塞流以再次冷却晶体。 （参考：高分子低温晶体学新技术：高分子晶体退火和低温氦，B. Leif Hansona，Constance A. Schallb 和 Gerard J. Bunick，Journal of Structural Biology
第 142 卷，第 1 期，2003 年 4 月，第 77-87 页）

尝试用液氮清洗晶体。 使用移液器小心地将液氮吸入移液器中，然后在晶体上滴几滴甚至是轻柔的液流并安装。 液氮清洗也可用于去除盖子、销钉和环上的爬行冰。