Hampton Pct™预结晶试验 PCT™ Pre-Crystallization Test

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Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test

APPLICATIONS

Determine the appropriate sample concentration for crystallization screening

FEATURES

Conserve sample
Optimize sample concentration prior to initial screens
Provide insight to sample homogeneity

DESCRIPTION

The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.

The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.

Each PCT kit contains 4 unique reagents, 50 ml each. This kit is useful for researchers who already have crystallization plates and cover slides

Each PCT kit (with plates) contains 4 unique reagents, 30 ml each, plus 24 well VDX Plates with sealant and siliconized cover slides. This kit is useful for labs without access to crystallization plates and cover slides.

应用

确定适宜的结晶筛选样品浓度

特征

保存样本
初始筛选前优化样品浓度
提供样本同质性的洞察力

描述

采用PCT预结晶试验，确定适合结晶筛选的蛋白质浓度.样品浓度是一个重要的结晶变量。样品浓度过高会导致无定形沉淀，而样品过稀则会产生明显的落点。沉淀和澄清滴是典型的结晶筛选结果，在试剂条件下不促进结晶，是每个结晶筛的一部分。然而，通过优化屏幕上的蛋白质浓度，可以减少清晰和沉淀结果的数量，从而提高样品的利用率，同时增加结晶的机会。Pct可以最大限度地减少或防止屏幕导致大量沉淀或清除滴落的情况。

PCT试剂盒包含4种独特的、预先配制的、无菌过滤试剂，用于评估用于结晶筛选的蛋白质浓度。最初，样品蛋白与两种试剂混合，以确定蛋白质浓度是否适合结晶筛选。如果蛋白质对盐和聚合物浓度非常敏感，根据最初的PCT结果，可以使用第二组PCT试剂对该蛋白质进行评估。然后，pct结果将提供对适当样品浓度的洞察力，或表明其他诊断测试，如自然凝胶电泳或动态光散射，应用于证明适合结晶的样品均匀性。

每个PCT试剂盒包含4种独特的试剂，每个50毫升。这个工具包对于已经有晶化板和盖片的研究人员是有用的。

每个PCT试剂盒(带板)包含4种独特的试剂，每个30毫升，加上24孔VDX板，带有密封剂和硅化盖板。这个试剂盒是有用的实验室，没有接触结晶板和盖幻灯片。

HR2-140 PCT 50 ml bottles (4 ea), cover slides (1 pk)
HR2-142 PCT (with plates) 30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)