Molecular Dimensions MD1-25 MemSys

Molecular Dimensions MD1-25 MemSys

A systematic screen spanning the key values of pH, precipitant type / concentration and salts for alpha-type transmembrane proteins using vapour diffusion methods. 48 conditions allowing the pH range, precipitants and salts used in membrane protein crystallization to be screened with detergent containing protein drops. The reagents can be easily arranged in systematic array to facilitate the interpretation of results and the design of further optimization experiments.

MemSys™

Features of MemSys™:

  • A systematic approach to screening for initial crystallization conditions for membrane proteins using vapour diffusion methods.
  • Membrane protein solubility is pushed to the limit to provide more information than previous sparse matrix type screens.
  • Includes the pH, precipitant concentration and type, and salts found to be successful.
  • Primarily designed for alpha-type transmembrane proteins, but has also been successfully applied to beta-type outer membrane proteins.

Developed by members of the So Iwata Lab, Imperial College, London. This product is manufactured under an exclusive licence from Imperial College of Science, Technology & Medicine, London, UK.

使用蒸汽扩散方法对 α 型跨膜蛋白的 pH 值、沉淀剂类型/浓度和盐的关键值进行系统筛选。 48 种条件允许使用含有蛋白质滴剂的洗涤剂筛选膜蛋白质结晶中使用的 pH 值范围、沉淀剂和盐。试剂可以很容易地排列在系统阵列中,以方便结果的解释和进一步优化实验的设计。

MemSys™ 的特点:

使用蒸汽扩散方法筛选膜蛋白初始结晶条件的系统方法。
膜蛋白溶解度被推到极限,以提供比以前稀疏矩阵类型屏幕更多的信息。
包括 pH 值、沉淀剂浓度和类型以及成功的盐类。
主要设计用于 α 型跨膜蛋白,但也已成功应用于 β 型外膜蛋白。
由伦敦帝国理工学院 So Iwata 实验室的成员开发。本产品在英国伦敦帝国理工学院的独家许可下生产。

References   参考文献
“Methods and Results in the crystallization of Membrane proteins” (2003) Ed. Iwata S. (International University Line)

Literature Citing use of Product
1. Gabdulkhakov AG, Fufina TY, Vasilieva LG, Mueller U, Shuvalov VA. Expression, purification, crystallization and preliminary X-ray structure analysis of wild-type and L(M196)H-mutant Rhodobacter sphaeroides reaction centres. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013; 69(Pt 5): 506-509
2. Nagarathinam K, Jaenecke F, Nakada-Nakura Y, et al. The multidrug-resistance transporter MdfA from Escherichia coli: crystallization and X-ray diffraction analysis. Acta Crystallogr F Struct Biol Commun. 2017; 73(Pt 7): 423-430
3. Zabara, A., Chong, J.T.Y., Martiel, I. et al. Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains. Nat Commun. 2018; 9: 544
4. Lin, Y. Crystallization and preliminary X-ray diffraction studies of melibiose permease. bioRxiv. 2020; 173740
Product # Product Name Unit Size
MD1-04 The Membrane Protein Combination 96 x 10 ml
MD11-60-33 Hanging Drop Starter Kit MemStart™ + MemSys™ HT-96 1 kit
MD1-25 MemSys™ 48 x 10 ml
MD1-33 MemStart™ + MemSys™ HT-96 96 x 1 ml
MDSR-25 MemSys™ Single Reagent 100 ml
MDSR-33 MemStart™ + MemSys™ HT-96 Single Reagent 100 ml