Hampton research HR2-096 Silver Bullets
Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
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Optimization Screens > Silver Bullets > Silver Bullets
应用
- 蛋白质晶体优化的添加剂筛选
- 用于可溶性蛋白和膜蛋白
- 添加屏幕以发现不同的晶体形式
- 传统筛网不成功时的二次或正交结晶筛
- 蛋白质溶解度和稳定性优化的添加剂筛选
特征
- 汉普顿研究公司
- 筛选一系列小分子和辅料，以确定它们是否有能力建立稳定、分子间、氢键、疏水和静电相互作用，从而促进晶格的形成和结晶。
- 小有机盐和有机酸
- 生物活性小分子
- 氨基酸肽
- 大分子消化
- C0-因子及配体
- 生化途径中间体
- 核苷酸，药典，碳水化合物
描述
银子弹筛选一组小分子的能力，以建立稳定，分子间，氢键，疏水和静电相互作用，可以促进稳定性，晶格形成和结晶。1-3
银子弹已经发表的结果是非常令人鼓舞的，超过两倍以上的蛋白质被结晶的整体是不受任何小分子控制的结晶。1-3
X射线衍射分析揭示了晶格中的小分子银子弹存在于氢键网络中心和静电相互作用中心。1-3银弹适用于悬挂式、坐式和三明治式滴水蒸气扩散法、微批法、自由界面法和微透析晶化法。银子弹可用于动态光散射(DLS)、热荧光和尺寸排除色谱分析。
银子弹试剂组合
有机盐和酸
生物活性小分子
·氨基酸和肽
·大分子消化
银子弹试剂盒是一个小分子库，已经被证明可以促进晶格的形成。X射线衍射分析表明，该试剂具有：
·稳定蛋白质的构象
·干扰蛋白质与溶剂的相互作用
·参与形成重要的晶格接触
·通过在晶体中的大分子间形成可逆的交联来建立晶格
银子弹试剂盒是由96种溶液组成的单深井块(Greiner 786261块规格：总体积：0.5mL工作体积：0.03-0.7mL，室温0.03-0.55mL，在-20ďż˝C，井剖面：锥形(V)，底部：固体，板色：半透明)，HT格式。
在0.02MHEPES钠pH6.8缓冲液中，每种银子弹试剂都是小分子或大分子消化的混合物。每个溶液包含2到20个小分子。
银子弹试剂体积为0.5ml(每口井)。
热氟是强生公司的注册商标。
APPLICATIONS
- Additive screen for the optimization of protein crystals
- For use with soluble proteins and membrane proteins
- Additive screen to discover different crystal forms
- Secondary or orthogonal crystallization screen when traditional screens are not successful
- Additive screen for the optimization of protein solubility and stability
FEATURES
- Developed at Hampton Research
- Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization
- Small organic salts and organic acids
- Biologically active small molecules
- Amino acids & peptides
- Macromolecular digests
- C0-factors & ligands
- Biochemical pathway intermediates
- Nucleotides, pharmacaphores, & carbohydrates
DESCRIPTION
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ďż˝C, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.
HR2-096 Silver Bullets 0.5 ml, Deep Well block format
RELATED ITEM(S)
Individual Silver Bullets Reagents