分类目录归档：Hampton Optimization Screen

Hampton 蛋白结晶优化试剂 Optimization Screens

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Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性

Protein specific agents for optimization of solubility & stability  

2、Solubility & Stability Screen 2   离子缓冲液

Buffer, pH & ionic strength screen for optimization of solubility & stability

3、Slice pH    PH缓冲液

pH & buffer screen for optimization of crystals or sample solubility & stability

4、Additive Screen  96孔板添加剂试剂盒

96 compound screen for optimization of crystal size & quality

5、Silver Bullets • Silver Bullets Bio

Small molecule additive screen for optimization of crystal size & quality

6、Detergent Screen

Detergent screen for optimization of crystals or sample solubility & stability

7、Ionic Liquid Screen

Ionic liquid additive screen for optimization of crystal size & quality

8、StockOptions Buffer Kits

Titrated buffer sets for optimization of crystals or sample solubility & stability

9、StockOptions Polymer

n of crystal size & quality

10、StockOptions Salt

Salt reagent kit for optimization of crystal size & qualit

11、Polymer reagent kit for optimizatioy

Proti-Ace

In situ proteolysis kit protein for crystallization screening & optimization

12、Reductive Alkylation

Reductive alkylation kit for crystallization screening & optimization

13、Silica Hydrogel Kit

pH neutral silica hydrogel matrix crystallization

14、Low Melting Agarose

Agarose gel matrix crystallization

15、CryoPro

Water soluble cryoprotectant reagent set

16、Heavy Atom Screens

Heavy atom kits for multiple isomorphous replacement

17、I3C Phasing Kit

Produce heavy atom derivative of biological macromolecules for phasing

Hampton StockOptions pH Buffer Kit

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Hampton StockOptions pH Buffer Kit

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > StockOptions Buffer Kits > StockOptions pH Buffer Kit

APPLICATIONS

  • Crystallization grade buffer stocks for crystal screening, optimization, & production

FEATURES

  • Titrated, preformulated, & ready-to-use buffers
  • pH 2.2 – 11.0
  • 1.0 M concentrated stocks (0.5 M for CAPSO and CAPS due to solubility limits)
  • Citric acid, Sodium acetate trihydrate, Sodium citrate tribasic dihydrate, Sodium cacodylate trihydrate, HEPES sodium, Tris hydrochloride, CAPSO, and CAPS buffers

DESCRIPTION

StockOptions reagent kits are reagent tool boxes for the macromolecular crystal grower. They offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in these kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits such as Crystal Screen™ and Crystal Screen 2™. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

StockOptions pH is a convenient and complete kit with buffers pHed from 2.2 to 11.0 in increments of 0.2 pH units. Each reagent is available in convenient concentration, making crystal setups quick and easy. Gone is the tedious task of finding and sourcing reagents, as well as costly and time-consuming reagent formulation.

The generation of custom screens or optimized conditions now simply involves pipetting StockOptions pH reagents from one convenient kit. This portfolio of buffers between pH 2.2 and 11, along with wide arrays of salts, polymers, and organic solvents also stimulates creativity since all of the tools are readily and conveniently available. All one needs to do is select the reagent and pipet.

The convenience also reduces the chance of errors. Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.

StockOptions pH is cost-effective. When a StockOptions kit is purchased, one is using reagents as preformulated stocks in reasonable volumes. You buy only the reagents you need, not a large container of raw material that may sit out on shelves for years to come. When you need larger volumes, Hampton Research offers individual, preformulated, sterile filtered Optimize™ crystallization reagents which include salts, polymers, organics solvents, and buffers. Also, one can special order crystallization reagents through the Hampton Research Custom Shop. Optimize, Custom Shop, StockOptions, and all Hampton Research kits are synergistic research tools.

HR2-241 StockOptions pH Kit pH 2.2 to 11.0 in 0.2 pH unit increments

RELATED ITEM(S)
Individual StockOptions pH Reagents

Hampton HR2-859 聚乙二醇 600 Polyethylene glycol 600

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Hampton HR2-859 聚乙二醇 600 Polyethylene glycol 600

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimize Reagents > Optimize – Polymers > Polyethylene glycol 600

APPLICATIONS

  • Crystallization grade Polyethylene glycol 600 for formulating screens or for optimization

FEATURES

  • HR2-859 100% Polyethylene glycol is a waxy solid at room temperature
  • HR2-860 80% w/v Polyethylene glycol is a solution at room temperature
  • HR2-860 is sterile filtered and formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

Polyethylene glycol 600

Synonyms: PEG 600
H(OCH2CH2)nOH
Mr 570-630
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24888888
Vapor density >1 (vs air)
Vapor pressure <0.01 mmHg (20°C)
Autoignition temperature 581°F
Refractive index (lit.) n20/D 1.468-1.470
Melting point 17-22°C
HR2-859 100% Polyethylene glycol is a waxy solid at room temperature
HR2-860 80% w/v Polyethylene glycol is a solution at room temperature
Solubility in water: soluble to 80% v/v at 25°C, clear, colorless
Density (100%) 1.13 g/mL at 20°C

HR2-859 Measured Refractive Index Range: 1.46874 – 1.46951 at 20°C
HR2-859 Measured Conductivity Range: 2.9 – 3.6 µS/cm at 25°C

HR2-860 Measured Refractive Index Range: 1.43676 – 1.46951 at 20°C
HR2-860 Measured Conductivity Range: 21.5 – 24.6 µS/cm at 25°C

Total impurities as insoluble matter: passes filter test
Peroxides (as H2O2) ≤0.001%
Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K  ≤0.02%
Li  ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.02%
Ni  ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

HR2-859 100% Polyethylene glycol 600 100 mL
HR2-860 80% w/v Polyethylene glycol 600 100 mL

Hampton Solubility & Stability Screen

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Hampton Solubility & Stability Screen

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > Solubility & Stability Screen > Solubility & Stability Screen

APPLICATIONS

  • A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
  • Preformulation screening
  • Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR

FEATURES

  • Compatible with Thermofluor, Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
  • 96 sterile filtered reagents
  • 17 classes of reagents and excipients
  • Highly concentrated (10x) reagent formulation
  • Developed at Hampton Research

DESCRIPTION

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11 The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15 Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

HR2-072 Solubility & Stability Screen 0.5 ml, Deep Well block format

Hampton research HR2-096 Silver Bullets

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Hampton research HR2-096 Silver Bullets

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > Silver Bullets > Silver Bullets

应用

  • 蛋白质晶体优化的添加剂筛选
  • 用于可溶性蛋白和膜蛋白
  • 添加屏幕以发现不同的晶体形式
  • 传统筛网不成功时的二次或正交结晶筛
  • 蛋白质溶解度和稳定性优化的添加剂筛选

特征

  • 汉普顿研究公司
  • 筛选一系列小分子和辅料，以确定它们是否有能力建立稳定、分子间、氢键、疏水和静电相互作用，从而促进晶格的形成和结晶。
    • 小有机盐和有机酸
    • 生物活性小分子
    • 氨基酸肽
    • 大分子消化
    • C0-因子及配体
    • 生化途径中间体
    • 核苷酸，药典，碳水化合物

描述

银子弹筛选一组小分子的能力，以建立稳定，分子间，氢键，疏水和静电相互作用，可以促进稳定性，晶格形成和结晶。1-3

银子弹已经发表的结果是非常令人鼓舞的，超过两倍以上的蛋白质被结晶的整体是不受任何小分子控制的结晶。1-3

X射线衍射分析揭示了晶格中的小分子银子弹存在于氢键网络中心和静电相互作用中心。1-3银弹适用于悬挂式、坐式和三明治式滴水蒸气扩散法、微批法、自由界面法和微透析晶化法。银子弹可用于动态光散射(DLS)、热荧光和尺寸排除色谱分析。

银子弹试剂组合
有机盐和酸
生物活性小分子
·氨基酸和肽
·大分子消化

银子弹试剂盒是一个小分子库，已经被证明可以促进晶格的形成。X射线衍射分析表明，该试剂具有：
·稳定蛋白质的构象
·干扰蛋白质与溶剂的相互作用
·参与形成重要的晶格接触
·通过在晶体中的大分子间形成可逆的交联来建立晶格

银子弹试剂盒是由96种溶液组成的单深井块(Greiner 786261块规格：总体积：0.5mL工作体积：0.03-0.7mL，室温0.03-0.55mL，在-20ďż˝C，井剖面：锥形(V)，底部：固体，板色：半透明)，HT格式。

在0.02MHEPES钠pH6.8缓冲液中，每种银子弹试剂都是小分子或大分子消化的混合物。每个溶液包含2到20个小分子。

银子弹试剂体积为0.5ml(每口井)。

热氟是强生公司的注册商标。

APPLICATIONS

  • Additive screen for the optimization of protein crystals
  • For use with soluble proteins and membrane proteins
  • Additive screen to discover different crystal forms
  • Secondary or orthogonal crystallization screen when traditional screens are not successful
  • Additive screen for the optimization of protein solubility and stability

FEATURES

  • Developed at Hampton Research
  • Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization
    • Small organic salts and organic acids
    • Biologically active small molecules
    • Amino acids & peptides
    • Macromolecular digests
    • C0-factors & ligands
    • Biochemical pathway intermediates
    • Nucleotides, pharmacaphores, & carbohydrates

DESCRIPTION

Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ďż˝C, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

HR2-096    Silver Bullets 0.5 ml,    Deep Well block format

RELATED ITEM(S)
Individual Silver Bullets Reagents

Hampton research Additive Screen • Additive Screen HT

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Hampton research Additive Screen • Additive Screen HT

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > Additive Screen > Additive Screen • Additive Screen HT

APPLICATIONS

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

FEATURES

  • 18 classes of reagents
  • Highly concentrated (10x) reagent formulation
  • 96 unique reagents and excipients
  • Tube or Deep Well block format
  • Crystallization or sample solubility optimization

DESCRIPTION

Additive Screen is a library of reagents that can affect the solubility and crystallization of biological macromolecules, including both soluble and membrane proteins.

These reagents can perturb and manipulate sample-sample and sample-solvent interactions, as well as perturb water structure which can alter and improve both the solubility and crystallization of a sample. Additives can stabilize or engender conformity by specific interaction with the macromolecules. There are numerous reports of the use of additives to improve the quality and size of macromolecular crystals.1-5

Additive screen reagent classifications include multivalent, salt, amino acid, dissociating agent, linker, polyamine, osmolyte, chaotrope, co-factor, reducing agent, chelating agent, polymer, carbohydrate, non-detergent, amphiphile, detergent, non-volatile organic and volatile organic.

Additive Screen contains 96 unique reagents, 1 ml each.

Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block.

添加剂筛·添加剂筛HT

应用

  • 操纵样品-样品和样品-溶剂的相互作用，以改善晶体或改变样品的溶解度。

特征

  • 18类试剂
  • 高浓度(10倍)试剂配方
  • 96种独特试剂和辅料
  • 管式或深井块格式
  • 结晶或样品溶解度优化

描述

添加剂筛选是影响生物大分子(包括可溶性蛋白质和膜蛋白)溶解度和结晶性的试剂库。

这些试剂可以干扰和操纵样品-样品和样品-溶剂的相互作用，也可以改变和改善样品的溶解度和结晶性的扰动水结构。添加剂可以通过与大分子的特定相互作用来稳定或产生整合。有许多关于使用添加剂来提高大分子晶体质量和尺寸的报道。1-5

添加剂筛选试剂可分为多价、盐、氨基酸、解离剂、连接剂、多胺、渗透压剂、壳聚糖、辅助因子、还原剂、螯合剂、聚合物、碳水化合物、非洗涤剂、两亲性试剂、洗涤剂、非挥发性有机和挥发性有机化合物.

添加剂筛选包含96个独特的试剂，每个1毫升。

添加剂筛选HT包含96个独特的试剂，每个1毫升，在一个单一的深井区块。

HR2-428 Additive Screen 1 ml, tube format
HR2-138 Additive Screen HT 1 ml, Deep Well block format

RELATED ITEM(S)
Individual Additive Screen Reagents

Hampton research HR2-245 StockOptions Salt

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Hampton research HR2-245 StockOptions Salt

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > StockOptions Salt > StockOptions Salt

应用领域

  • 用于筛分配方和优化的结晶级盐试剂原液

特征

  • 预先配制和无菌过滤
  • 从筛选到优化的轻松过渡
  • 与Hampton Research筛选，试剂盒和试剂协同作用
  • 49种独特的盐，包括有机酸
  • 高度浓缩，随时可以稀释

描述

StockOptions试剂盒是用于大分子晶体种植者的试剂工具箱。他们以方便，经济的试剂盒提供精确配制的高质量结晶级试剂储备。这些试剂盒中使用的化学药品是汉普顿研究试剂盒中使用的相同结晶度，超高纯度化学药品，例如Crystal Screen™和Crystal Screen 2™。 StockOptions试剂在严格的质量标准下精心配制，以确保可靠的性能和批次间的一致性。

StockOptions Salt

APPLICATIONS

  • Crystallization grade salt reagent stock solutions for screen formulation and optimization

FEATURES

  • Preformulated and sterile filtered
  • Easy transition from screening to optimization
  • Synergistic with Hampton Research screens, kits, & reagents
  • 49 unique salts, including organic acids
  • Highly concentrated, ready to dilute

DESCRIPTION

StockOptions reagent kits are reagent tool boxes for the macromolecular crystal grower. They offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in these kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits such as Crystal Screen™ and Crystal Screen 2™. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

HR2-245 StockOptions Salt Kit 10 ml, tube format

Hampton HR2-523 Polyethylene glycol 1000

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Hampton HR2-523 Polyethylene glycol 1000

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimize Reagents > Optimize – Polymers > Polyethylene glycol 1,000

应用

  • 结晶级聚乙二醇1,000用于筛选或优化

特征

  • 无菌过滤液
  • 在1+超纯水中配制：在25°C下电阻率为18.2兆，总有机碳<5 ppb，无细菌(<1细菌(CFU/ml))，无热原(<0.03内毒素(EU/ml))，无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

描述

聚乙二醇1,000

APPLICATIONS

  • Crystallization grade Polyethylene glycol 1,000 for formulating screens or for optimization

FEATURES

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

Polyethylene glycol 1,000

HR2-523 50% w/v Polyethylene glycol 1,000 200 mL