上海同步辐射装置 (SSRF) –蛋白解决loop环、底座、puck选型指南

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上海同步辐射装置 (SSRF) –蛋白解决loop环、底座、puck选型指南

BEAMLINE GUIDE  选型指南
SSRF BL17U1

Shanghai Synchrotron Radiation Facility
Pudong, Shanghai, China
ACTOR Automounter

BL17U at Shanghai Synchrotron Radiation Facility (SSRF) is a PX beamline designed to high resolution and high throughput structural determination of macromolecules and their complexes. The energy range for BL17U is 5-18 keV. The sample changing robot, ACTOR of Rigaku, is installed. The cassette is Uni-Puck and one puck can holds 16 protein crystals.1

上海同步辐射设施
浦东，上海，中国
自动加载器的演员

上海同步辐射设施(SSRF)的BL17U是一种用于高分辨率、高通量大分子及其配合物结构测定的PX光束线。BL17U的能量范围为5- 18kev。安装了Rigaku的ACTOR换样机器人。盒式是Uni-Puck，一个puck可以容纳16个蛋白质晶体

OTHER LINKS

  • Beamline Website
  • Contact Beamline
  • Contact MiTeGen

Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style Uni-Puck Uni-Puck, ALS, ACTOR†
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B1A-Reusable B1A-Reusable, B1-R, B3-R, B3S-R, B5-R
*Remote Access of the beamline BL17U at Shanghai Synchrotron Radiation Facility†To purchase ACTOR pucks, please contact us.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

光束线样品制备套件可根据您的晶体尺寸提供3种孔径尺寸组合，包括:

100水晶安装组件，18毫米引脚，可在以下分类:
20 x双厚度MicroLoops™LD(孔径尺寸分别为10、20、30和50 μm各5个)
40 x双厚度MicroMount™(各10个75,100,150和200 μm孔径尺寸)
20 x双厚度MicroLoops™(孔径大小分别为300、400、500和600 μm各5个)
20 x MicroMeshes™(孔径尺寸分别为10、25、50和25L μm各5个)
安装在100个B5-R可重复使用基地或100个常规基地
包装和存储在5个MiTeGen基础持有人

 

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.