Hampton 洗涤试剂Detergent Screen

Detergent Screen
96 detergent formulations for sample and crystal optimization

Detergent Screen

A tetragonal crystal of AppA, a photoreceptor from Rhodobacter sphaeroides, grown in Hampton Research Detergent Screen. Vladimira Dragnea, Indiana University.

Crystal of beta-lactamase grown in the presence of detergent. James Knox, University of Connecticut

Applications   应用

Manipulate sample-sample and sample-solvent interactions, including non-specific aggregation due to hydrophobic interactions, to alter sample solubility, or obtain or improve crystalline samples.
操纵样品-样品和样品-溶剂的相互作用，包括由于疏水相互作用而产生的非特异性聚集，以改变样品的溶解度，或获得或改善结晶样品。

Features 特征
Developed at Hampton Research  汉普顿公司研究开发
For use with either membrane or soluble proteins  用于膜或可溶性蛋白质
Compatible with vapor diffusion, microbatch, and free interface diffusion methods         兼容蒸汽扩散，微批和自由界面扩散方法。
96 Deep Well block format     96深井区块格式
Classification of Detergents in screen  筛分中洗涤剂的分类
Ionic  离子
Non-ionic  非离子
Zwitterionic  两性离子
Non-detergent Sulfobetaines  非洗涤剂磺酸盐

Description
Note: HR2-407 Detergent Screen is a new formulation and has replaced the original HR2-406 Detergent Screen HT.

For crystallization to take place, a protein must be soluble in aqueous solution. Ideally the sample should be homogeneous, monodisperse, and in a state of aggregation conducive to interactions which will promote the nucleation and subsequent growth of a crystal. Unfavorable aggregation can compete with, obstruct, and prevent the normal ordering or the sample into a crystal. Many proteins, typically membrane proteins, membrane associated proteins, and soluble proteins contain hydrophobic residues on the surface which can lead to non-specific aggregation, a deterrent to solubility and crystallization. Mild, biological detergents can perturb and manipulate hydrophobic sample-sample and sample-solvent interactions. Incorporating detergents into the solubilization or crystallization reagent during screening and optimization is a popular and effective strategy for identifying conditions which promote and enhance solubility and crystallization. Besides improving the crystallization properties for some proteins and nucleic acids, detergents have also been shown to alleviate problems of twinning and secondary nucleation and can also produce different crystal forms.

Since it can be difficult to impossible a priori to predict and select which detergent or solubilizing agent will minimize the general nonspecific interactions yet at the same time sustain the specific interactions necessary for crystallization, the screening of a portfolio of detergent and solubilizing reagents is an efficient and effective strategy to identify the appropriate and best detergent reagent. The Detergent Screen is a set of solubilization and crystallization specific, mild, biological detergent reagents in a ready to use format at concentrations appropriate for solubility and crystallization screens. The Detergent Screen format allows one to identify a detergent effect as well as help select the best detergent reagent.

Each Detergent Screen kit contains 96 unique detergents. 0.25 ml of detergent solution is formulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered deionized water. The Detergent Screen kit contains 96 solutions in a single Deep Well block, a single sheet of AlumaSeal II Sealing Film, User Guide, Formulations, and Scoring Sheet.

Detergent Screen HT Individual Reagents  0.5 ml

Crystal of alpha amylase

Membrane protein crystallized with beta D-glucopyranoside. Karl Brillet, IREBS, Illkirch, France.

Membrane protein crystallized with CHESO (2- hydroxyethyloctylsulfoxide. Karl Brillet, IREBS, Illkirch, France.

Applications

Individual Detergent Screen reagents for screening, optimization

Features
Individual reagents from the original HR2-406 Detergent Screen HT
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

0.5 ml volume
Description
Individual reagents from the original HR2-406 Detergent Screen HT supplied in a volume of 0.5 milliliter in a sterile polypropylene tune with o-ring seal. All detergents formulated in deionized, sterile filtered Type 1 Laboratory Water. Container is purged with argon prior to closure.

Detergent Screen Individual Reagents
0.5 ml
MembFac • Crystal Screen Lite • MembFac HT
Primary biased sparse matrix crystallization screen for membrane proteins and proteins with limited solubility