Hampton 透析按钮 Dialysis Buttons

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Hampton 透析按钮 Dialysis Buttons

Applications
Crystallization by dialysis, protein folding, & small volume sample dialysis

Features
Low volume dialysis
Fits in 24 well plates
Use over and over again

Methodologies
Controlled crystal dehydration.4

Description 
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3 Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

应用

透析结晶，蛋白质折叠，小容量样品透析

特征

低容量透析
适用于24孔板
一遍又一遍地使用

方法

控制晶体脱水
描述 
在透析法中，样品被半透膜从“沉淀剂”中分离出来，这种膜允许小的试剂分子通过，但防止生物大分子穿过膜。1-3透析按钮是注射成型的聚苯乙烯，大小与一个小按钮。样品被放置在这个房间，以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部，并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米，外径约为10毫米而没有O形环，以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米，外径约为15毫米，没有O形环，而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头，以协助将透析膜应用到按钮上，并与所提供的o-环固定在一起。

Hampton 透析按钮 Dialysis Buttons

1 Related Item  相关产品信息
Applicator for Dialysis Buttons

References  参考文献
1.McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2.Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3.Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4.Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.