标签归档：Hampton 蛋白质结晶

A is for Aggregation 阻止包括蛋白质、肽和核酸在内的生物大分子的结晶

发表于由

A is for Aggregation  A 代表聚合

Aggregation can be a deterrent to the crystallization of biological macromolecules including proteins, peptides, and nucleic acids. The presence of sample aggregation can be detected by either dynamic light scattering or native gel electrophoresis. Aggregation might be caused by hydrophobic patches on the surface of the sample, differently charged isoforms, differently phosphorylated isoforms, mixtures of methylated and non-methylated samples, glycosylation, as well as electrostatic interactions. Aggregation can be due to autologous aggregation where the protein is aggregating with itself or heterologous contamination where the sample is aggregating with other proteins. In the case of heterologous contamination, further purification of the sample should be seriously considered. In the case of autologous aggregation that precludes crystallization one might consider:

Using molecular biology to manipulate intra and inter molecule interactions by modifying the sample sequence (alter, add, or delete residues).

Use chemical additives to manipulate sample-sample and sample solvent interactions.
Detergents
Chaotropes (urea, guanidine hydrochloride, hydrochloric acid, etc)
Electrostatic agents
Alcohols (isopropanol, methanol, ethanol, etc)
Salts (sodium chloride, potassium chloride, sodium fluoride, etc)
Polyols (glycerol, PEG 400, etc)
Ligands, inhibitors, co-factors, and metals
Use temperature to prevent aggregation (0°C and 60°C)
Consider a fusion protein
Remove C-terminus or N-terminus
Truncate domains
Remove His-tag

In some cases aggregates can be removed by centrifugation or filtration.

In some cases the aggregates can be removed by mixing the sample with the crystallization reagent, allowing the sample to incubate for 15 minutes, centrifuging the sample/reagent mixture, removing the precipitate and setting the drop with the supernatant.

聚集可以阻止包括蛋白质、肽和核酸在内的生物大分子的结晶。可以通过动态光散射或天然凝胶电泳检测样品聚集的存在。聚集可能是由样品表面的疏水斑块、不同电荷的异构体、不同的磷酸化异构体、甲基化和非甲基化样本的混合物、糖基化以及静电相互作用引起的。聚集可能是由于蛋白质与自身聚集的自体聚集或样品与其他蛋白质聚集的异源污染。在异源污染的情况下，应认真考虑样品的进一步纯化。在排除结晶的自体聚集的情况下，可以考虑：

使用分子生物学通过修改样品序列（改变、添加或删除残基）来操纵分子内和分子间的相互作用。

使用化学添加剂来控制样品-样品和样品溶剂的相互作用。
洗涤剂
离液剂（尿素、盐酸胍、盐酸等）
静电剂
醇类（异丙醇、甲醇、乙醇等）
盐类（氯化钠、氯化钾、氟化钠等）
多元醇（甘油、PEG 400 等）
配体、抑制剂、辅因子和金属
使用温度防止聚集（0°C 和 60°C）
考虑融合蛋白
移除 C 端或 N 端
截断域
删除 His 标签

在某些情况下，可以通过离心或过滤去除聚集体。

在某些情况下，可以通过将样品与结晶试剂混合、让样品孵育 15 分钟、离心样品/试剂混合物、去除沉淀物并用上清液放置液滴来去除聚集体。

Crystal Screen as an additive screen 附加屏幕

发表于由

Crystal Screen as an additive screen 附加屏幕

Although Hampton Research does offer a specifically formulated Additive Screen (HR2-428), here is a tip when one already has a sparse matrix screen or two laying about the lab. When screening additives try adding 50 microliters of each Crystal Screen reagent to 950 microliters the “best” crystallization conditions thus far in order to see if any of the reagents in Crystal Screen might serve as good additives. Crystal Screen 2 is an especially good kit for this technique since Crystal Screen 2 contains numerous divalent cations, Jeffamine® Reagent, and a few other “novel” agents. Jeffamine® is a registered trademark of the Huntsman Petrochemical Corporation

Reference 参考文献

Crystallization of foot-and-mouth disease virus 3C protease: surface mutagenesis and a novel crystal-optimization strategy. Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):646-50. Epub 2005 Apr 20. Birtley JR1, Curry S.

水晶屏幕作为附加屏幕
尽管 Hampton Research 确实提供了一种专门配制的添加剂屏幕 (HR2-428)，但当一个人在实验室周围已经有一个或两个稀疏矩阵屏幕时，这里有一个提示。 当筛选添加剂时，尝试将 50 微升的每种 Crystal Screen 试剂添加到迄今为止“最佳”结晶条件的 950 微升中，以查看 Crystal Screen 中的任何试剂是否可以作为良好的添加剂。 Crystal Screen 2 是该技术特别好的套件，因为 Crystal Screen 2 包含大量二价阳离子、Jeffamine® 试剂和一些其他“新型”试剂。 Jeffamine® 是 Huntsman Petrochemical Corporation 的注册商标。

 

Hampton HR2-795 1.0 M BIS-TRIS propane pH 7.0

发表于由

Hampton HR2-795 1.0 M BIS-TRIS propane pH 7.0

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

BIS-TRIS propane Buffer

APPLICATIONS  应用

  • Crystallization grade BIS-TRIS propane pH 7.0 for formulating screens or for optimization   结晶级 BIS-TRIS 丙烷 pH 7.0，用于配制筛网或优化

FEATURES   特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

DESCRIPTION

BIS-TRIS propane

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
Mr 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C

HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C

HR2-795        1.0 M BIS-TRIS propane pH 7.0         100 mL

RELATED ITEM(S) 相关产品

REFERENCES  参考文献

1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.

2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.

Hampton 丙二酸钠 3.4 M Sodium malonate pH 4.0

发表于由

Hampton 丙二酸钠 3.4 M Sodium malonate pH 4.0

Sodium malonate  丙二酸钠

APPLICATIONS  应用

  • Crystallization grade Sodium malonate for formulating screens or for optimization用于筛选或优化的结晶级丙二酸钠

FEATURES  特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤液
  • 在1+超纯水中配制：在25°C下电阻率为18.2兆，总有机碳<5 ppb，无细菌(<1细菌(CFU/ml))，无热原(<0.03内毒素(EU/ml))，无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

DESCRIPTION

The Sodium malonate offered by Hampton Research is Malonic acid (CAS Number 141-82-2) titrated to pH 4.0-8.0 using Sodium hydroxide as described in the publication “A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.”. In this same publication, Sodium malonate was reported to be more successful than any other salt, resulting in the crystallization of 19 of the 23 macromolecules, almost twice as effective as the next most successful salt, which was a draw between sodium acetate, sodium tartrate, sodium formate, and ammonium sulfate. The high success rate of Sodium malonate in producing crystals was even more impressive when an overall unique success rate with individual macromolecules was considered.

The use of Sodium malonate in place of Ammonium sulfate has been reported in one instance to improve the reproducibility of crystals and improve resistance to physicochemical shocks. Sodium malonate also acted as a good cryoprotectant (Xing and Xu 2003).

One may combine the four different sodium malonate stocks to create a pH versus sodium malonate concentration grid for screening or optimization. Refer to the pH/Dilution Table for Sodium malonate.

Based on the structure of malonic acid titrated with sodium hydroxide, there are no chiral carbons so it is not characterized as having enantiomeric structures, such as D or L.

Synonyms: Propanedioic acid or Malonic acid
C3H4O4 (before titration with NaOH)
Mr 104.06 (before titration with NaOH)
CAS Number 141-82-2
EC Number 205-503-0
Beilstein Registry Number 1751370
Merck 14,5710
RTECS OO0175000
MDL Number MFCD00002707
Purity > 99.0%
pKa1 2.8
pKa2 5.7

描述

Hampton研究提供的丙二酸钠是苹果酸(化学文摘社编号141-82-2)，使用氢氧化钠滴定至pH4.0-8.0，载于出版物“大分子结晶盐的比较”，Alexander McPherson，“蛋白质科学”(2001)，10：418-422。在同一份出版物中，据报道，丙二酸钠比任何其他盐都更成功，导致23种大分子中的19种结晶，几乎是第二种最成功的盐的两倍，即乙酸钠、酒石酸钠、甲酸钠和硫酸铵之间的结晶。当考虑到单个大分子的整体独特成功率时，丙二酸钠在晶体生产中的高成功率更令人印象深刻。

有报道称，用丙二酸钠代替硫酸铵可提高晶体的重现性，提高其抗物理化学冲击的能力。丙二酸钠也是一种良好的冷冻保护剂(邢和旭，2003年)。

一种方法是将四种不同的丙二酸钠组合起来，形成一个pH值相对于丙二酸钠浓度的网格，用于筛选或优化。请参阅丙二酸钠的pH/稀释表。

根据氢氧化钠滴定丙二酸的结构，不存在手性碳，不具有对映体结构，如D或L。

同义词：丙二酸或丙二酸
C3H4O4(用氢氧化钠滴定前)
Mr104.06(用氢氧化钠滴定前)
核证机关编号141-82-2
欧共体号码205-503-0
伯尔斯坦登记处编号1751370
默克14,5710
RTECS OO 0175000
MDL号码MFCD 00002707
纯度>99.0%
PKA 1 2.8
PKa25.7

HR2-747 Measured Conductivity Range: 73.3 – 75.3 mS/cm at 25°C
HR2-747 Measured Refractive Index Range: 1.38365 – 1.38425 at 20°C

HR2-749 Measured Conductivity Range: 77.2 – 78.0 mS/cm at 25°C
HR2-749 Measured Refractive Index Range: 1.39264 – 1.39300 at 20°C

HR2-751 Measured Conductivity Range: 73.4 – 75.2 mS/cm at 25°C
HR2-751 Measured Refractive Index Range: 1.39938 – 1.39973 at 20°C

HR2-707 Measured Conductivity Range: 71.0 – 73.7 mS/cm at 25°C
HR2-707 Measured Refractive Index Range: 1.40090 – 1.40098 at 20°C

HR2-807 Measured Conductivity Range: 71.8 – 75.0 mS/cm at 25°C
HR2-807 Measured Refractive Index Range: 1.40106 – 1.40115 at 20°C

HR2-747测量电导率范围：25°C下73.3~75.3 ms/cm
HR2-747测量折射率范围：1.38365~1.38425℃，20°C

HR2-749在25°C下的电导率范围：77.2~78.0 ms/cm
HR2-749测量折射率范围：1.39264~1.39300℃，20°C

HR2-751测量电导率范围：25°C下73.4~75.2 ms/cm
HR2-751测量折射率范围：1.39938~1.39973℃，20°C

HR2-707测量电导率范围：25°C下71.0~73.7 ms/cm
HR2-707测量折射率范围：1.40090~1.40098℃，20°C

HR2-807测量电导率范围：25°C下71.8~75.0 ms/cm
HR2-807测量折射率范围：1.40106~1.40115℃，20°C

HR2-747 3.4 M Sodium malonate pH 4.0 200 mL
HR2-749 3.4 M Sodium malonate pH 5.0 200 mL
HR2-751 3.4 M Sodium malonate pH 6.0 200 mL
HR2-707 3.4 M Sodium malonate pH 7.0 200 mL
HR2-807 3.4 M Sodium malonate pH 8.0 200 mL

REFERENCES  参考文献

1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

2. Crystallization of the PX domain of cytokine-independent survival kinase (CISK): improvement of crystal quality for X-ray diffraction with sodium malonate. Yi Xing and Wenqing Xu. Acta Cryst. (2003). D59, 1816-1818.

3. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko. Acta Cryst. (2003). D59, 2356-2358.

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase. Kim et al Acta Cryst. (2007). F63, 579–581.

Hampton HR2-539 四水酒石酸钾钠缓冲液

发表于由

Hampton 四水酒石酸钾钠缓冲液

Potassium sodium tartrate tetrahydrate  四水酒石酸钾钠

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

APPLICATIONS 应用

  • Crystallization grade Potassium sodium tartrate tetrahydrate for formulating screens or for optimization 四水酒石酸钾结晶级筛选或优化

FEATURES  特征

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤液
  • 在1+超纯水中配制：在25°C下电阻率为18.2兆，总有机碳<5 ppb，无细菌(<1细菌(CFU/ml))，无热原(<0.03内毒素(EU/ml))，无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

DESCRIPTION

Potassium sodium tartrate tetrahydrate

Synonyms: L(+)-Tartaric acid potassium sodium salt, or Rochelle salt, or Seignette salt
KOCOCH(OH)CH(OH)COONa • 4H2O
C4H4KNaO6 • 4H2O
Mr 282.22
CAS Number [6381-59-5]
EC Number 206-156-8
Beilstein Registry Number 6113568
MDL Number MFCD00150989
PubChem Substance ID 24881874
Purity ≥ 99.5%

Measured pH Range: 8.4 – 9.1 at 25°C
Measured Conductivity Range: 81.1 – 98.5 mS/cm at 25°C
Measured Refractive Index Range: 1.37544 – 1.37666 20°C

描述

四水酒石酸钾钠

同义词：l(+)-酒石酸钾钠盐，或罗谢尔盐，或塞格奈特盐
KOCOCH(OH)CH(OH)Coona·4H2O
C4H4KNaO6 ·4H2O
Mr 282.22
核证机关编号[6381-59-5]
欧共体编号206-156-8
伯尔斯坦登记处编号6113568
MDL号码MFCD 00150989
PubChem物质ID 24881874
纯度≥99.5%

测量pH值范围：8.4-9.1℃，25°C
测量电导率范围：81.1-98.5ms/cm，25°C
测量折射率范围：1.37544-1.37666 20°C

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
N ≤0.002%
Ni ≤0.0005%
Pb ≤0.0005%
PO4 ≤0.001%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

HR2-539 1.5 M Potassium sodium tartrate tetrahydrate 200 mL

 

REFERENCES 参考文献

1. Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3. K. Miyazono, N. Kudo and M. Tanokura. Acta Cryst. (2004). D60, 1135-1136.

2. Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides. Johansson P, Denman S, Brumer H, Kallas AM, Henriksson H, Bergfors T, Teeri TT, Jones TA. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):535-7.

Hampton 酒石酸铵 Ammonium tartrate dibasic

发表于由

Hampton 酒石酸铵 Ammonium tartrate dibasic

Ammonium tartrate dibasic  酒石酸铵

APPLICATIONS  应用

  • Crystallization grade Ammonium tartrate dibasic for formulating screens or for optimization  结晶级酒石酸铵，用于筛选或优化

FEATURES  特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤液
  • 在1+超纯水中配制：在25°C下电阻率为18.2兆，总有机碳<5 ppb，无细菌(<1细菌(CFU/ml))，无热原(<0.03内毒素(EU/ml))，无RNase(<0.01 ng/mL)和无DNase(<4 pg/L)

DESCRIPTION

Ammonium tartrate dibasic

Synonym: L-(+)-Tartaric acid diammonium salt or Diammonium tartrate
(NH4)2C4H4O6
C4H12N2O6
Mr 184.15
CAS Number [3164-29-2]
EC Number 221-618-9
Beilstein Registry Number 6120352
RTECS WW8050000
MDL Number MFCD00013073
PubChem Substance ID 24846510
Purity ≥ 99.5%

HR2-679 Measured pH Range: 6.3 – 6.9 at 25°C
HR2-679 Measured Conductivity Range: 118.2 – 134.4 mS/cm at 25°C
HR2-679 Measured Refractive Index Range: 1.39169 – 1.39346 at 20°C

HR2-767 titrated to pH 7.0 using NaOH at 25°C
HR2-767 Measured Conductivity Range: 115.7 – 129.6 mS/cm at 25°C
HR2-767 Measured Refractive Index Range: 1.38057 – 1.38157 at 20°C

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.0005%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%

描述

酒石酸铵

同义词：l-(+)-酒石酸二胺盐或酒石酸二铵
(NH)4)2C4H4O6
C4H12N2O6
Mr 184.15
核证机关编号[3164-29-2]
教统会编号221-618-9
伯尔斯坦登记处编号6120352
RTECS WW8050000
MDL号码MFCD00013073
PubChem物质ID 24846510
纯度≥99.5%

HR2-679测定pH值范围：6.3~6.9℃，25°C
HR2-679在25°C下的电导率范围：118.2~134.4 ms/cm。
HR2-679测量折射率范围：1.39169~1.39346℃，20°C

HR2-767在25℃NaOH滴定至pH7.0
HR2-767测量电导率范围：25°C下115.7~129.6 ms/cm。
HR2-767测量折射率范围：1.38057~1.38157℃，20°C

Al≤0.0005%
AS≤0.00001%
BA≤0.0005%
Bi≤0.0005%
CA≤0.0005%
CD≤0.0005%
CL≤0.005%
CO≤0.0005%
Cr≤0.0005%
Cu≤0.0005%
Fe≤0.0005%
K≤0.005%
Li≤0.0005%
Mg≤0.0005%
Mn≤0.0005%
Mo≤0.0005%
NA≤0.005%
Ni≤0.0005%
PB≤0.0005%
所以4≤0.005%
Sr≤0.0005%
Zn≤0.0005%

HR2-679 2.0 M Ammonium tartrate dibasic 200 mL
HR2-767 1.6 M Ammonium tartrate dibasic pH 7.0 200 mL

REFERENCES  参考文献

1. Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa. J. Y. Ha, J. H. Lee, K. H. Kim, D. J. Kim, H. H. Lee, H.-K. Kim, H.-J. Yoon and S. W. Suh. Acta Cryst. (2006). F62, 139-141.

Hampton盖玻片 Cover Slides（Siliconized Glass & Plastic Slides）

发表于由

Hampton盖玻片 Cover Slides（Siliconized Glass & Plastic Slides）

Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools >

12 mm Siliconized Glass Cover Slides

Images

12 mm硅化玻璃盖板滑片

应用

  • 用于悬挂和坐滴结晶的硅化玻璃盖板滑片
  • 悬滴结晶
  • 坐滴结晶
  • 夹层滴结晶

特征

  • 高纯玻璃
  • 硅化的、疏水性玻璃表面
  • 0.22毫米及0.96毫米玻璃厚度
  • 12毫米玻璃直径
  • 圆
  • 0.22毫米玻璃厚度与紫外线兼容(透射率>85%)

描述

硅化玻璃覆盖幻灯片允许一个液滴悬浮在一个位置，为周围的储油层溶液的蒸汽扩散提供了近乎佳的条件。

这些硅化玻璃覆盖幻灯片提供了一个一致的，高质量的完成结晶实验。疏水性表面会产生一滴“很好”的水滴，在玻璃上不会变平。硅化表面防止晶体粘附并沉淀到玻璃表面。使用陶氏康宁真空润滑脂(HR3-510)或陶氏康宁7释放化合物(HR3-508)密封滑动板。可在12，18或22毫米直径圆和22毫米直径方格。有0.22毫米或0.96毫米玻璃厚度。0.96毫米厚的幻灯片在晶化处理过程中几乎是不可破碎的。

临界表面张力未处理玻璃78 dynes/cm硅化玻璃31 dynes/cm表面电阻率未处理玻璃1×1012欧姆硅化玻璃1.2×1013欧姆摩擦系数静态，玻璃玻片上未经处理的0.9-1.0硅化0.2-0.3蛋白吸附(100小时)未处理玻璃0.13mg/mm2硅化玻璃0.01-0.02 mg/mm2

以下幻灯片为紫外线可传输(>85%)：
HR3-277和HR3-279-12毫米x 0.22毫米硅化圆盖幻灯片
HR3-231和HR3-233-22毫米x 0.22毫米硅化圆盖幻灯片
HR3-239和HR3-241-18毫米x 0.22毫米硅化圆盖幻灯片
HR3-215和HR3-217-22毫米x 0.22毫米硅化方盖幻灯片

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

FEATURES

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 12 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

DESCRIPTION

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

The following slides are UV transmissible (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

CAT NO NAME DESCRIPTION
HR3-278T Siliconized circle cover slides for Tecan robot 0.5 oz pack (~240 slides)
HR3-277 12 mm x 0.22 mm Siliconized circle cover slides 0.5 ounce pack (~240 slides)
HR3-279 12 mm x 0.22 mm Siliconized circle cover slides 5.0 ounce case (~2,400 slides)
HR8-088 12mmx0.96mmThick Siliconized circle cover slides 1.0 ounce pack (~106 slides)
HR8-090 12mmx0.96mm Thick Siliconized circle cover slides 10.0 ounce case (~1,060 slides)
18 mm Siliconized Glass Cover Slides

Images

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization
CAT NO NAME DESCRIPTION
HR3-239 18 mm x 0.22 mm Siliconized circle cover slides 0.5 ounce pack (~100 slides)
HR3-241 18 mm x 0.22 mm Siliconized circle cover slides 5.0 ounce case (~1,000 slides)
HR3-515 18mmx0.96mm Thick Siliconized circle cover slides 1 oz pack (~45 slides)
HR3-517 18mmx0.96mm Thick Siliconized circle cover slides 10 oz case (~450 slides)
22 mm Siliconized Glass Cover Slides

Images

APPLICATIONS

  • Siliconized glass cover slides for hanging & sitting drop crystallization.
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization
CAT NO NAME DESCRIPTION
HR3-231 22 mm x 0.22 mm Siliconized circle cover slides 1.0 ounce pack (~120 slides)
HR3-233 22 mm x 0.22 mm Siliconized circle cover slides 10.0 ounce case (~1,200 slides)
HR3-247 22 mm x 0.96 mm Thick Siliconized circle cover slides 3 ounce pack (~75 slides)
HR3-249 22mmx 0.96mm Thick Siliconized circle cover slides 30.0 ounce case (~750 slides)
HR3-215 22 mm x 0.22 mm Siliconized square cover slides 1.0 ounce pack (~100 slides)
HR3-217 22 mm x 0.22 mm Siliconized square cover slides 10.0 ounce case (~1,000 slides)
HR3-223 22mmx0.96mm Thick Siliconized square cover slides 4.0 ounce pack (~75 slides)
HR3-225 22mmx0.96mm Thick Siliconized square cover slides 40.0 ounce case (~750 slides)

Images

Glass Cover Slide Gizmo Dispenser

Images

Siliconized Glass Coverslips (Greiner)

Images

OptiClear Plastic Cover Slides

Images

PEN-VAC Kit

Images

Cover Slide Vacuum Gadget

Hampton 96孔蛋白结晶板Crystallization Plate (Swissci)

发表于由

Hampton 96孔蛋白结晶板Crystallization Plate (Swissci)

Crystallization Plates, Hardware & Accessories | 96 Well Crystallization Plates

Images

MRC Under Oil 96 Well

Images

MRC 2 Well UVP

Images

MRC 2 Well UVXPO
3 Well Midi Crystallization Plate

Images

3 Well Low Profile Crystallization Plate
LCP Lipidic Cubic Phase Screening
Diaplate

Hampton 96 孔蛋白结晶板Crystallization Plate (Swissci)

Diaplate
96 well 3.2 µl capacity dialysis plate

CrystalEX (Corning)
Sitting drop crystallization

CrystalEX Second Generation (Corning)
Sitting drop crystallization

CrystalQuick 96 Well Sitting Drop Plate (Greiner)
Sitting drop crystallization

Intelli-Plate 96 (Art Robbins Instruments)
Sitting drop crystallization

MRC 2 Well Crystallization Plate (Swissci) UVXPO
Sitting drop crystallization UVXPO

MRC 2 Well Crystallization Plate (Swissci) UVP
Sitting drop crystallization UVP

3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

FRAP Sandwich Set
FRAP Sandwich Set

LCP Sandwich Set
Crystallization screening of membrane proteins in lipidic mesophase as well as bicelle method and batch method

LCP Lipidic Cubic Phase Screening plate (Swissci)
Lipidic cubic phase crystallization

MRC Under Oil 96 Well Crystallization Plate (Swissci)
Microbatch crystallization

Imp@ct Plate without reservoir (Greiner)
Microbatch crystallization

MASTERBLOCK® 96 Deep Well Plate (Greiner)
Reagent storage

Microplate Lid
Cover for 96 well HT Screens and Masterblock

Hampton 96 孔蛋白结晶板Crystallization Plate (Swissci)

3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

CAT NO NAME DESCRIPTION
HR3-123 3 Well Midi Crystallization Plate (Swissci) 10 plate case
HR3-125 3 Well Midi Crystallization Plate (Swissci) 40 plate case
HR3-205 3 Well Low Profile Crystallization Plate (Swissci) 10 plate case
HR3-206 3 Well Low Profile Crystallization Plate (Swissci) 40 plate case

MRC 2 Well Crystallization Plate (Swissci) UVP

Sitting drop crystallization UVP

CAT NO NAME DESCRIPTION
HR3-082 MRC 2 Well Crystallization Plate 10 plate case
HR3-083 MRC 2 Well Crystallization Plate 40 plate case

MRC 2 Well Crystallization Plate (Swissci) UVXPO
Sitting drop crystallization UVXPO

CAT NO NAME DESCRIPTION
HR3-106 MRC 2 Well Crystallization Plate in UVXPO 10 plate case
HR3-107 MRC 2 Well Crystallization Plate in UVXPO 40 plate case

MRC Under Oil 96 Well Crystallization Plate (Swissci)
Microbatch crystallization

Applications
Microbatch crystallization

Features
Easy crystal retrieval
Easy viewing
UV imaging compatible
Drop Volume 100 nanoliter to 4 microliter
Oil Volume 20 µl
SBS Standard
Ultra low binding polymer, no static

Description 
The MRC Under Oil 96 Well Crystallization Plate is designed for microbatch crystallization.

The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, United Kingdom) in collaboration with Dr. Jan Löwe and colleagues. It is a result of many years of experience in successful robotic high-throughput crystallization and combines many of the features not earlier available to the crystallographer.

The new MRC Under Oil 96 Well Crystallization Plate is designed for microbatch crystallization using paraffin, silicon oil, or a mixture of the two. Following the initial experiment, evaporation of the drop through the oil allows for second crystallization stage, enabling further crystal growth as a consequence of concentration. This is different from other experiments in that the conditions are then extreme in nature and permit new conditions to arise.

The new MRC Under Oil 96 Well Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from an optically superior polymer and with a new design of the wells, the plate allows easy crystal viewing and retrieval.

The advantages of the new MRC Under Oil 96 Well Crystallization Plate.

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.

Easy Viewing
The wells are wide conical and have a polished surface on both sides important for perfect illumination. The micro numbering ensures that you will never get lost again (visible by microscope). The optically superior polymer is even UV transmissible and may be used to differentiate between salt and crystals.

Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape for the initial experiments of microbatch. No central bending occurs in this very robust structure.

Recommended Volumes
Typical volumes validated for these plates are 20 µl of oil with a shot through sample delivery of 100 nanoliter to 4 microliter.

SBS Standard
The plates are designed to the 96 well SBS standard for all common holders and external numbering (A – H, 1 – 12) with corner location that make the plate easy to use in a robotic sampler. The plate can also be centrifuged for better results. The unique MRC Under Oil 96 well Crystallization plate offers a new way of microbatch crystallography. The 96 wells are optically perfect, designed to observe crystals under a microscope.

Unique Polymer
The proprietary polymer is optically perfect – ultra low binding and guaranteed to have central drop location in the well. There are no static effects and thus micro-droplet jumping is avoided.

CAT NO NAME DESCRIPTION
HR3-102 MRC Under Oil Crystallization Plate 10 plate case
HR3-104 MRC Under Oil Crystallization Plate 40 plate case

Hampton 结晶Loop环底座 CrystalCap

发表于由
Hampton 结晶Loop环底座 CrystalCap
CrystalCap™ SPINE HT
Images

CrystalCap™ SPINE HT

APPLICATIONS

  • Cryocrystallography

FEATURES

  • SPINE style sample mount for cryocrystallography
  • CrystalCap SPINE HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Hollowed out design compatible with SPINE style grippers, auto mounters and sample handlers
  • Compatible with automated sample handlers
  • 2D alphanumeric coding for sample tracking & management
  • Bar coded, color coded, alphanumeric, magnetic cap
  • Color coded CryoLoop size

CrystalCap™ SPINE HT
应用
低温晶体学
特征
用于冷冻晶体学的 SPINE 型样品架
CrystalCap SPINE HT 以磁性方式连接到 CrystalCap 样品瓶和磁性测角仪底座
镂空设计与 SPINE 型夹持器、自动贴片机和样品处理器兼容
与自动化样品处理器兼容
用于样本跟踪和管理的 2D 字母数字编码
条形码、颜色编码、字母数字、磁性帽
颜色编码的 CryoLoop 尺寸

CrystalCap™
Images
  • Cryocrystallography

FEATURES

  • SSRL style sample mount for cryocrystallography
  • CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

低温晶体学
特征
用于低温晶体学的 SSRL 型样品架
CrystalCap 磁性连接到 CrystalCap 小瓶和磁性测角仪底座
圆锥形状，带有与 SSRL 型夹持器、自动贴片机和样品处理器兼容的壁架

CrystalCap™ ALS
Images

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers

应用
低温晶体学
特征
用于低温晶体学的 ALS 型晶体支架
CrystalCap ALS 以磁性方式连接到 CrystalCap 样品瓶和磁性测角仪底座
与 ALS 型夹持器、自动贴片机和样品处理器兼容的无边缘圆锥形状

CrystalCap™ ALS HT
Images

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
  • 2D alphanumeric coding for sample tracking & management
  • Color coded CryoLoop size

应用
低温晶体学
特征
用于低温晶体学的 ALS 型晶体支架
CrystalCap ALS HT 磁性连接到 CrystalCap 样品瓶和磁性测角仪底座
与 ALS 型夹持器、自动贴片机和样品处理器兼容的无边缘圆锥形状
用于样本跟踪和管理的 2D 字母数字编码
颜色编码的 CryoLoop 尺寸

Cap Container empty

Sample Containers:

A: SSRL cassette

 

B: ACTOR magazine

 

C: Cryo-vial basket or EMBL/ESRF cryo-vial basket

 

D: ALS puck

E: Uni-puck

F: miniSPINE puck

Sample Pin Bases:

A: Hampton magnetic

 

B: Hampton copper magnetic

 

C: ALS-style

 

C: ALS-style

 

D: Mitegen B3S

E: SPINE

F: miniSPINE

Hampton蛋白结晶工具及耗材

特色

发表于由

Hampton蛋白结晶工具及耗材

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Hampton蛋白质结晶捞针（结晶环）18 mm Mounted CryoLoop™ – 20 micron

HR4-974 18 mm Mounted CryoLoop 0.7 - 1.0 mm

HR4-974

Mounted CryoLoop™ – 20 micron

APPLICATIONS

  • Cryocrystallography

HR4-965 Mounted CryoLoop 0.7 - 1.0 mm

HR4-965

Hampton 蛋白结晶loop环 Mounted CryoLoop 10 micron with EasySnap MicroTube

HR4-994 Mounted CryoLoop 0.025 - 0.05 mm

CAT NO NAME DESCRIPTION
HR4-994 Mounted CryoLoop – 10 micron 0.025-0.05 mm – 25 pack
HR4-995 Mounted CryoLoop – 10 micron 0.05 – 0.1 mm – 25 pack
HR4-997 Mounted CryoLoop – 10 micron 0.1 – 0.2 mm – 25 pack
HR4-999 Mounted CryoLoop – 10 micron 0.2 – 0.3 mm – 25 pack

Adjustable Mounted CryoLoop™ with EasySnap MicroTube

HR8-110 Adjustable Mounted CryoLoop 0.7 - 1.0 mm

Applications
Cryocrystallography
Features
Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop

Description 
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.

Using forceps or a probe one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.

CAT NO NAME DESCRIPTION
HR4-338 Adjustable Mounted CryoLoop 0.05 – 0.1 mm – 25 pack
HR5-900 Adjustable Mounted CryoLoop Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)
HR4-336 Adjustable Mounted CryoLoop 0.025 – 0.05 mm – 25 pack
HR8-102 Adjustable Mounted CryoLoop 0.1 – 0.2 mm – 25 pack
HR8-072 Adjustable Mounted CryoLoop 0.2 – 0.3 mm – 25 pack
HR8-104 Adjustable Mounted CryoLoop 0.3 – 0.4 mm – 25 pack
HR8-106 Adjustable Mounted CryoLoop 0.4 – 0.5 mm – 25 pack
HR8-108 Adjustable Mounted CryoLoop 0.5 – 0.7 mm – 25 pack
HR8-110 Adjustable Mounted CryoLoop 0.7 – 1.0 mm – 25 pack

Hampton蛋白结晶小瓶和结晶瓶帽 CrystalCap™

CrystalCap™ ALS

Applications  应用
Cryocrystallography   低温晶体学
Features  特征
ALS style crystal mount for cryocrystallography
CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
用于低温晶体学的 ALS 型晶体支架
CrystalCap ALS 以磁性方式连接到 CrystalCap 样品瓶和磁性测角仪底座
与 ALS 型夹持器、自动贴片机和样品处理器兼容的无边缘圆锥形状

Description 
The CrystalCap ALS is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

CrystalCap ALS (HR4-779) is supplied without a vial (HR4-904).

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS is compatible with ALS style grippers, auto mounters and sample handlers.

ALS style sample mounters accept the CrystalCap ALS.

HR4-779    CrystalCap ALS     without Vial – 60 pack

APPLICATIONS

  • Cryocrystallography

FEATURES

  • ALS style crystal mount for cryocrystallography
  • CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers

CrystalCap

APPLICATIONS

  • Cryocrystallography

FEATURES

  • SSRL style sample mount for cryocrystallography
  • CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
  • Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers
CAT NO NAME DESCRIPTION
HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

CrystalCap & Vial

Hampton CrystalCap™ SPINE HT

Applications  应用
Cryocrystallography   低温晶体学

Features   特征
SPINE style sample mount for cryocrystallography
CrystalCap SPINE HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base

Hollowed out design compatible with SPINE style grippers, auto mounters and sample handlers

2D alphanumeric coding for sample tracking & management
Bar coded, color coded, alphanumeric, magnetic cap
Color coded CryoLoop size

用于低温晶体学的 SPINE 型样品架
CrystalCap SPINE HT 以磁性方式连接到 CrystalCap 样品瓶和磁性测角仪底座

镂空设计与 SPINE 型夹持器、自动贴片机和样品处理器兼容

用于样本跟踪和管理的 2D 字母数字编码
条形码、颜色编码、字母数字、磁性帽
颜色编码的 CryoLoop 尺寸

Description
The CrystalCap SPINE HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SPINE style caps. The CrystalCap SPINE HT attaches to a magnetic CrystalCap SPINE Vial and magnetic goniometer base. The tip of the CrystalCap SPINE HT accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap SPINE Vial is a 1.8 ml cryo vial featuring two small vents and is compatible with the CrystalCap SPINE HT. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The CrystalCap SPINE Vial features chamfered edges for enhanced cap positioning as well as a magnetic alloy bottom for stability. The HR8-114 CrystalCap SPINE Vial does have a magnet on the bottom of the vial.

The hollowed out design of the CrystalCap SPINE HT is compatible with SPINE style grippers, auto mounters and sample handlers.

The CrystalCap SPINE HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CAT NO NAME DESCRIPTION
HR8-118 CrystalCap SPINE HT 0.025-0.05 mm CryoLoop, without Vial – 30 pack
HR8-120 CrystalCap SPINE HT 0.05-0.1 mm CryoLoop, without Vial – 30 pack
HR8-122 CrystalCap SPINE HT 0.1-0.2 mm CryoLoop, without Vial – 30 pack
HR8-124 CrystalCap SPINE HT 0.2-0.3 mm CryoLoop, without Vial – 30 pack
HR8-126 CrystalCap SPINE HT 0.3-0.4 mm CryoLoop, without Vial – 30 pack
HR8-128 CrystalCap SPINE HT 0.4-0.5 mm CryoLoop, without Vial – 30 pack
HR8-130 CrystalCap SPINE HT 0.5-0.7mm CryoLoop, without Vial – 30 pack
HR8-132 CrystalCap SPINE HT 0.7-1.0 mm CryoLoop, without Vial – 30 pack
HR8-112 CrystalCap SPINE HT Cap only – 30 pack
HR8-116 CrystalCap SPINE HT Cap/Vial Cap/Vial only – 30 pack
HR8-094 CrystalCap SPINE without 2D code/printing Cap only – 30 pack
HR8-114 CrystalCap SPINE Vial Vial only – 30 pack

CrystalCap™ ALS HT

Applications  应用

Cryocrystallography  低温晶体学

Features  特征
ALS style crystal mount for cryocrystallography
CrystalCap ALS HT attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers
2D alphanumeric coding for sample tracking & management
Color coded CryoLoop size

用于低温晶体学的 ALS 型晶体支架
CrystalCap ALS HT 磁性连接到 CrystalCap 样品瓶和磁性测角仪底座
与 ALS 型夹持器、自动贴片机和样品处理器兼容的无边缘圆锥形状
用于样本跟踪和管理的 2D 字母数字编码
颜色编码的 CryoLoop 尺寸


Description
The CrystalCap ALS HT is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS HT attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS HT is compatible with ALS style grippers, auto mounters and sample handlers.

The CrystalCap ALS HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CAT NO NAME DESCRIPTION
HR8-200 CrystalCap ALS HT 0.025-0.05 mm CryoLoop – 30 pack
HR8-202 CrystalCap ALS HT 0.05-0.1 mm CryoLoop – 30 pack
HR8-204 CrystalCap ALS HT 0.1-0.2 mm CryoLoop – 30 pack
HR8-206 CrystalCap ALS HT 0.2-0.3 mm CryoLoop – 30 pack
HR8-208 CrystalCap ALS HT 0.3-0.4 mm CryoLoop – 30 pack

Hampton 石英毛细管 Quartz Capillaries

Applications  应用

X-ray data collection   X射线数据采集
Liquid-liquid diffusion crystallization  液-液扩散结晶
Gel acupuncture crystallization  凝胶针灸结晶

Features  特征
Quartz  石英
Thin walled – 10 micron  薄壁 – 10 微米

CAT NO NAME DESCRIPTION
HR6-128 Quartz Capillary Size: 0.1 mm – 25 pack
HR6-130 Quartz Capillary Size: 0.2 mm – 25 pack
HR6-132 Quartz Capillary Size: 0.3 mm – 25 pack
HR6-134 Quartz Capillary Size: 0.4 mm – 25 pack
HR6-136 Quartz Capillary Size: 0.5 mm – 25 pack
HR6-138 Quartz Capillary Size: 0.6 mm – 25 pack
HR6-140 Quartz Capillary Size: 0.7 mm – 25 pack
HR6-142 Quartz Capillary Size: 0.8 mm – 25 pack
HR6-144 Quartz Capillary Size: 0.9 mm – 25 pack
HR6-146 Quartz Capillary Size: 1.0 mm – 25 pack
HR6-148 Quartz Capillary Size: 1.5 mm – 25 pack
HR6-150 Quartz Capillary Size: 2.0 mm – 25 pack
HR6-151 Quartz Capillary Size: 2.5 mm – 15 pack
HR6-175 Quartz Capillary Size: 3.0 mm – 15 pack
HR6-177 Quartz Capillary Size: 4.0 mm – 5 pack
HR6-179 Quartz Capillary Size: 5.0 mm – 5 pack
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

Applications 应用

Cryocrystallography 蛋白结晶学

Spearlab Dewars
HR4-676 Cryogenic Foam Dewar (130 ml) – Mini each
HR4-662 Cryogenic Foam Dewar (500 ml) – Small each
HR4-673 Cryogenic Foam Dewar (800 ml) – Standard each
HR4-674 Cryogenic Foam Dewar (1400 ml) – Large each
HR4-675 Cryogenic Foam Dewar (2 liter) – Tall each

Hampton 结晶板封口膜 ClearSeal Film™ and Applicator

Applications 应用

Sealing film used to seal sitting drop crystallization experiments  使用密封膜来密封座滴板结晶实验

Features  特征
1.High optical clarity, low auto-fluorescence
2.Easy to handle film; easy to remove liner
3.Pressure sensitive adhesive
4.For best results use specially designed applicator
5.UV transmissable, compatible with UV imaging systems
6.Low birefringence
7.Certified DNase-, RNase-, and nucleic-acid-free
8.Silicone adhesive is non-tacky until pressed against the sealing surface with the applicator
9.Chemically inert; no extractable except at extreme pH
10.Excellent chemical resistance (e.g. DMSO, Methanol, Acetonitrile)
11.Heat and cold resistant, recommended for temperatures from -70°C and 110°C

特征
高光学透明度、低自体荧光
膜易于处理 ；易拆卸衬垫
压力敏感粘合剂
为了获得佳效果 ， 请使用专门设计的涂抹器
传染性紫外、紫外成像系统兼容
低双折射
认证 DNase – ， RNase – ， 和核酸的游离
硅酮粘合剂是无粘性的 ， 直到压在密封表面与涂抹器
化学惰性的 ；除了极端 pH 不能提取优异的耐化学性 (如。DMSO 、甲醇、乙腈)
耐热耐寒 ， 温度从 – 70 ° C 至 110 ° C

Crystal Clear Sealing Film

APPLICATIONS  应用

  • Sealing film used to seal sitting drop crystallization experiments

密封膜用于密封坐滴结晶实验

FEATURES   特征

  • Fits SBS format microplates
  • No special applicator required
  • Optically clear

适合 SBS 格式的微孔板
无需特殊涂抹器
光学透明

DESCRIPTION  描述

Crystal Clear Sealing Film is an optically transparent sealing film for SBS format 24, 48, 96 and 384 well plates. The 2 mil +/-10% (0.05 mm) thick, optically clear, non-fluorescing polyester film with 1.0 – 1.3 mil (0.025 mm) thick acrylic custom adhesive is designed for the convenient and secure sealing of crystallization plates. A split, optically opaque 2.0 mil +/-10% polyester backing with two end tabs assures a clean uniform adhesive layer and allows for easy, accurate positioning on the plate. Before use, peel off the opaque center protective polyester backing to reveal the optically clear sealing film. Film length: 5.625″ +/- 0.015 Film width: 3.125″ +/- 0.015 End tabs: 0.375″ +/- 0.015 Perforations: 0.415″ +/- 0.015 from edge of film Corner radius: 3/32″. Free of detectable levels of DNase-, RNase- and Human/Mouse DNA contamination (PCR Certified), non-sterile. Recommended temperatures from -40 to +120°C. Not recommended for UV imaging.

Crystal Clear Sealing Film 是一种光学透明密封膜，适用于 SBS 格式 24、48、96 和 384 孔板。 2 mil +/-10% (0.05 mm) 厚、光学透明、无荧光的聚酯薄膜和 1.0 – 1.3 mil (0.025 mm) 厚的丙烯酸定制粘合剂专为方便和安全地密封结晶板而设计。带有两个末端标签的分离、光学不透明的 2.0 mil +/-10% 聚酯背衬可确保清洁均匀的粘合剂层，并允许在板上轻松、准确地定位。使用前，撕下不透明的中央保护聚酯背衬，露出光学透明的密封膜。胶片长度：5.625″ +/- 0.015 胶片宽度：3.125″ +/- 0.015 端片：0.375″ +/- 0.015 穿孔：距胶片边缘 0.415″ +/- 0.015 角半径：3/32″。不可检测DNase-、RNase- 和人类/小鼠 DNA 污染（PCR 认证）的水平，非无菌。推荐温度为 -40 至 +120°C。不推荐用于紫外线成像。

Film Sealing Paddle  薄膜密封辅助板

APPLICATIONS  应用

  • Even and secure application of sealing films to crystallization plates   将密封膜均匀且安全地应用到结晶板上

FEATURES  特征

  • White, polyethyelene plastic
    • 5 paddles per pack
    • 2.958″ short side width, 3.250″ long side width, 0.125″ thick
    • Less firm than gold paddle
  • 白色，聚乙烯塑料
    每包 5 个桨
    2.958″ 短边宽，3.250″ 长边宽，0.125″ 厚
    不如金桨结实
  • Gold, plastic, nylon reinforced edge
    • 1 paddle per pack
    • 3 7/8″ wide x 2 15/16″ tall
    • More firm than white paddle
  • 金色、塑料、尼龙加强边
    每包 1 个桨
    3 7/8 英寸宽 x 2 15/16 英寸高
    比白桨更结实

HR4-413 Film Sealing Paddle – White 5 pack
HR4-525 3M Sealing Paddle – Gold Each

Hampton 石蜡和硅润滑油 Paraffin & Silicon Oil

Applications  应用
Microbatch crystallization    microbatch 结晶
Features  特征
Under oil crystallization 在油中结晶
Protect the sample from oxidation  保护样品免于氧化
Screen different temperatures without condensation   屏幕不同温度下不凝结
Description   描述 
Oils used for microbatch and modified microbatch crystallization under oil.

使用的油和改性 microbatch microbatch 结晶油。

Al’s Oil is a 50:50 (volume:volume) mixture of Paraffin Oil and Silicon Oil. Al’s Oil is named after it’s inventor, Allan D’Arcy.

Al 是机油的 50 ∶ 50 (体积 ∶ 体积) 混合物的石蜡油和硅油。Al 的石油是其发明者的名字命名 ， Allan D ‘Arcy) 。

Silicon Oil

Paraffin oil
Synonym: Mineral oil
CAS Number [8042-47-5]
EC Number 232-455-8
RTECS PY8047000
MDL Number MFCD00131611
Appearance Colorless, clear, viscous liquid
Refractive Index n20/D 1.467 (literature) n20/D 1.468
IR Spectroscopy Grade
Density 0.838 g/mL at 25°C (literature) 0.85 g/ml at 20°C
Viscosity 40-42 CST at 25°C

Silicon Oil 
Synonyms: Octamethyltrisiloxane
CAS Number: 107-51-7
Appearance: Colorless, clear viscous liquid
Melting Point: -86°C
Boiling Point: 152°C
Refractive Index: 1.38 at 25°C
Viscosity: 1 CST at 25°C
Specific Gravity: 0.82 at 25°C
Density: 0.82 g/mL at 25°C
Surface Tension: 17.4 mN/m

Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
HR4-670 Vial Clamp - Straight
HR4-670 Vial Clamp – Straight 结晶瓶夹子 each
CrystalWand™ Magnetic
Applications
CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool
结晶帽磁性&结晶帽铜磁性处理工具
Description
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.
HR4-729 CrystalWand Magnetic, Straight
HR4-315 CrystalWand Magnetic, 45° (McMiken Tool)
 
Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.
HR4-671 Vial Clamp – Curved 45°/135°
HR4-672 Vial Clamp – Curved 110°/70°
Cap Container (empty)

Applications

Holds 30 CrystalCap caps for storage or transport
Features
Black polypropylene box with overlap snap closure
Holds 30 caps with or without Mounted CryoLoops™
Description 
Empty black plastic box designed to hold 30 CrystalCap Copper Magnetic HT, CrystalCap Copper Magnetic ALS HT, CrystalCap HT (SPINE), CrystalCap Magnetic or CrystalCap Copper Magnetic without vials. Useful for shipping caps that do not need to be frozen or returning used caps home after a synchrotron trip. The Cap Container is designed for use with Caps only and does not accommodate vials.

HR8-016 Cap Container Opened

HR8-016 Cap Container empty  底座盒子 each

18 mm Siliconized Glass Cover Slides

APPLICATIONS 应用

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

用于悬挂和坐滴结晶的硅化玻璃盖玻片
悬滴结晶
坐滴结晶
夹心滴结晶

FEATURES  特征

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 18 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

玻璃纯度高
硅化疏水玻璃表面
0.22 毫米和 0.96 毫米玻璃厚度
18 毫米玻璃直径
圆圈
0.22 毫米玻璃厚度与紫外线兼容（> 85% 透射率）

12 mm Siliconized Glass Cover Slides HR3-278T Siliconized circle cover slides for Tecan robot
HR3-277 12 mm x 0.22 mm Siliconized circle cover slides
HR3-279 12 mm x 0.22 mm Siliconized circle cover slides
HR8-088 12mmx0.96mmThick Siliconized circle cover slides
HR8-090 12mmx0.96mm Thick Siliconized circle cover slides
22 mm Siliconized Glass Cover Slides HR3-231 22 mm x 0.22 mm Siliconized circle cover slides
HR3-233 22 mm x 0.22 mm Siliconized circle cover slides
HR3-247 22 mm x 0.96 mm Thick Siliconized circle cover slides
HR3-249 22mmx 0.96mm Thick Siliconized circle cover slides
HR3-215 22 mm x 0.22 mm Siliconized square cover slides
HR3-217 22 mm x 0.22 mm Siliconized square cover slides
HR3-223 22mmx0.96mm Thick Siliconized square cover slides
HR3-225 22mmx0.96mm Thick Siliconized square cover slides

Hampton 水晶透明密封带Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Hampton HR4-316胶 Super Glue

Loctite® Easy-Squeeze Super Glue Gel

APPLICATIONS

  • Staking Glass Fibers to Brass Specimen Pins.
  • Staking MicroTube to CrystalCap

FEATURES

  • Squeeze grips allow for precise application, keeping fingers glue free
  • Locking cap collar prevents leaks and drying out
  • Bonds more surfaces than ordinary glues, including: metal, CryoLoops, wood, paper, cardboard, vinyl, leather, rubber, cork, ceramic, china, textiles, brick and fingers.

CryoTong™ – Long
Applications

Crystal transfer under cryo temperature
Features

Artery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

CrystalCap™ Holder
applications
CrystalCap support inside stainless steel dewars
Features
Adjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.
HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool