Molecular Dimensions 蛋白结晶培养箱(立式)

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Temperature controlled cabinets specifically designed for crystal growth.

All have temperature programming and can be further customised with accessories such as lighting, glazed door, extra shelves, instrument cable ports and humidity control. These incubators need only a small floor space. They have a white interior with easily-cleaned plastic-coated wire shelves, adjustable at 35mm intervals to provide maximum flexibility of positioning. Temperature control is via an electronic controller with digital display, over/under temperature safety cut-outs and warning alarms. The doors are lockable and have a magnetic seal. The large workspace door can be opened wide to provide full-width access.

Molecular Dimensions Cooled Crystallisation Incubators are an economical alternative to building temperature-controlled rooms, and provide much more precise control of crystal growth temperature.

Features:

• 100, 160, 230, and 400-litre models
• Temperature range 0°C to 50°C
• Temperature accuracy ± 0.5°C for precise crystal growth conditions
• Virtually vibration free for undisturbed experiments
• Easy-to-use control panel
• Small foot-print maximises precious floor space in the laboratory

专为晶体生长而设计的温控柜。
所有产品都具有温度程序，并可进一步定制照明、玻璃门、额外搁板、仪器电缆端口和湿度控制等配件。这些孵化器只需要很小的占地面积。它们的内部为白色，带有易于清洁的塑料涂层金属丝架，可按 35 毫米的间隔进行调节，以提供最大的定位灵活性。温度控制是通过带有数字显示、超温/超温安全切断和警告警报的电子控制器进行的。门可上锁并带有磁性密封。大工作区门可以打开很宽，以提供全宽的访问。

分子尺寸冷却结晶培养箱是建造温控室的经济替代方案，可以更精确地控制晶体生长温度。

特征：

100、160、230 和 400 升型号
温度范围 0°C 至 50°C
温度精度 ± 0.5°C，适用于精确的晶体生长条件
对于不受干扰的实验几乎没有振动
易于使用的控制面板
占地面积小，可最大限度地利用实验室宝贵的占地面积

400 litre internal volume cooled crystallization incubator.

Precise and constant temperature
Temperature is controlled electronically. A continuously-running fan guarantees high accuracy of temperature control throughout the cabinet interior and over time, according to DIN-58945.
Heating and cooling
The continuously adjustable fan-mounted electrical resistance heater has low thermal “drag”. It is controlled by a solid-state relay, avoiding steps and relay contact breaking. This design provides maximal accuracy of temperature with “coasting” practically eliminated. The air-cooled refrigeration unit uses CFC-free refrigerant and has a highly efficient heat exchanger. Solenoid valve-bypass operation ensures smooth and continuous running, with minimal vibration.
Easy-to-use, programmable temperature control
A temperature program controller allowing up to 70 set points is fitted as standard. Programing is simple, with a clear digital display of set and actual temperature. Both real time and process time controlled dual set-point temperature programs are available. Set point values or a freely programable ramp function can be selected.
Double security for sample and incubator
The incubators cannot over-heat or over-cool. In the event of a failure, a standby controller with a separate sensor takes over temperature control. Additionally an alarm is sounded. A mechanical safety switch with factory-adjusted release temperature (TWB DIN12880, class 2) protects the incubator against incorrect adjustment of the temperature selector.

400 升内部容积冷却结晶培养箱。

精确恒温
温度由电子控制。根据 DIN-58945，连续运行的风扇可确保整个机柜内部和随时间推移的高精度温度控制。
加热和冷却
连续可调的风扇安装电阻加热器具有低热“阻力”。它由固态继电器控制，避免了步骤和继电器触点断开。这种设计提供了最大的温度精度，实际上消除了“惯性”。风冷式制冷机组采用无CFC制冷剂，具有高效换热器。电磁阀旁通操作确保平稳连续运行，振动最小。
易于使用的可编程温度控制
标配最多可设置 70 个设定点的温度程序控制器。编程简单，设置和实际温度的数字显示清晰。提供实时和过程时间控制的双设定点温度程序。可以选择设定点值或可自由编程的斜坡功能。
样品和培养箱双重安全
培养箱不能过热或过冷。在发生故障时，带有独立传感器的备用控制器接管温度控制。此外还会发出警报。带有出厂调节释放温度（TWB DIN12880，2 级）的机械安全开关可保护培养箱免受温度选择器的错误调节。

Molecular Dimensions MD1-13 3D Structure Screen 48 x 10 mL

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The 3D Structure Screen is formulated for growing protein crystals in the metastable zone using the vapour diffusion technique.

This method is presented in the 3D Structure Screen as an optimized sparse matrix crystal growth screen which allows the 24 “nucleation” conditions of the Mini Screen and 24 “backed-off” conditions for crystal growth to be tried. Formatted for HT blocks as the Heavy + Light (3D Structure Screen) Twin Pack HT-96.

Features of 3D Structure Screen:

Separate nucleation from crystal growth.
A simple method for growing crystals in the metastable zone.
Obtain much larger and better diffracting crystals compared to conventional methods.
This product is manufactured under an exclusive licence from Imperial College of Science, Technology and Medicine, London U.K.

3D Structure Screen 专为使用蒸汽扩散技术在亚稳态区域中生长蛋白质晶体而设计。

这种方法在 3D 结构屏幕中显示为优化的稀疏矩阵晶体生长屏幕，它允许尝试使用 Mini Screen 的 24 个“成核”条件和 24 个“回退”条件进行晶体生长。 为 HT 块格式化为 Heavy + Light（3D 结构屏幕）Twin Pack HT-96。

3D结构画面的特点：

将成核与晶体生长分开。
在亚稳区中生长晶体的简单方法。
与传统方法相比，获得更大更好的衍射晶体。
本产品是在英国伦敦帝国理工学院的独家许可下生产的。

References:  参考文献
Chayen, N. & Saridakis, E. Protein crystallization for genomics: towards high-throughout optimization techniques. Acta Cryst. D58: 921-927 (2002)
Chayen, N. & Saridakis, E. Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion. Protein Science 9(4): 755-757 (2000)
Charron, C et al. Crystallization and preliminary X-ray diffraction data of the second and archaebacterial-type aspartyl-tRNA synthetase from Thermus thermophilus. Acta Cryst. D57: 1177-1179 (2001)

Molecular Dimensions MD15-W12-T Wizard Classic 1 & 2

规格：96 x 10 ml

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The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof). The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.

Wizard™ Classic 系列随机稀疏矩阵筛选旨在提高在粗筛选阶段在结晶生物大分子（蛋白质、核酸、肽及其组合）时产生晶体的可能性。 事实证明，Wizard Classic 试剂是筛选生物大分子的高效起点。 Wizard Classic 配方包括多种结晶剂、缓冲剂和盐，涵盖了从 pH 4.5 到 pH 10.5 的广泛结晶空间。

Overall structure of the EcFtsZ(L178E)–GDP complex obtained with Wizard Classic 3 condition 354

Molecular Dimensions distributes Wizard Crystallisation kits on behalf of Rigaku Reagents.

References:
1. Ueda M, Shimosaka M, Arai R. Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis. Acta Crystallogr F Struct Biol Commun. 2015; 71(Pt 12): 1516-1520
2. Moriyama S, Nishio K, Mizushima, T. Structure of glyoxysomal malate dehydrogenase (MDH3) from Saccharomyces cerevisiae. Acta Cryst. 2018; F74: 617-624
3. Jeong, Y.C., Lee, K.S. Overexpression, purification, crystallization and preliminary X-ray crystallographic characterization of the receiver domain of the response regulator PhoP from Enterococcus faecalis ATCC 29212. Appl Biol Chem. 2019; 62, 66
4. Schumacher, MA, Ohashi, T, Corbin, L & Erickson, HP. High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP. Acta Cryst. 2020; 76(2): 94-102

Molecular Dimensions MD1-46 Morpheus 96 x 10 ml

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A 96 condition protein crystallization screen with original chemistry incorporating a range of low molecular weight ligands found ordered in more than 33000 PDB structures. Developed and utilized in the world-renowned Laboratory of Molecular Biology, (Cambridge, UK) – Morpheus® carries on the tradition of being original and dynamic. This unique screen aims to unlock novel chemical space previously inaccessible using conventional screens.

96 条件蛋白质结晶筛选与原始化学结合发现在超过 33000 个 PDB 结构中发现的一系列低分子量配体。 在世界著名的分子生物学实验室（英国剑桥）开发和使用 – Morpheus® 继承了原创和动态的传统。 这种独特的屏幕旨在解锁以前使用传统屏幕无法进入的新型化学空间。

An IUCr Webinar describing the formulation of the Morpheus® screens is available hereMolecular Dimensions, as well as a webinar describing the four-corner method for optimizing crystals obtained in Morpheus® conditions. The kit is presented as a 96 condition screen.

Features of Morpheus®:

• Simple and effective 3D grid design covering a range of pH, PEGs and salt additives.
• 49 low molecular weight ligands promote both initial crystal formation and lattice stability.
• Ideal for membrane proteins (contains PEGS and low salt)
• Reduced crystal “stress” – all conditions are cryo-protected.
• Derived from extensive data mining of over 33,000 PDB entries.
• Available in both 10 mL tube and 96 condition deep well block formats.

This product has been designed and developed by Fabrice Gorrec, in collaboration with the scientists at the Medical Research Council Laboratory of Molecular Biology (LMB) at Cambridge and is manufactured exclusively under license by Molecular Dimensions Limited.

References  参考文献

Witek, W., Sliwiak, J. & Ruszkowski, M. Structural and mechanistic insights into the bifunctional HISN2 enzyme catalyzing the second and third steps of histidine biosynthesis in plants. Sci Rep 11: 9647 (2021)

Kozak, S et al. Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis D76: 1244-1255 (2020)

Gorrec, F., & Löwe, J. Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology 131: 55790 (2018)

Sonani, Ravi R et al. An improved crystal structure of C-phycoerythrin from the marine cyanobacterium Phormidium sp. A09DM. 135(1): 65-78 (2018)

Gorrec, F. Protein crystallization screens developed at the MRC Laboratory of Molecular Biology 21(5): 819-825 (2016)
Gorrec, F. The MORPHEUS protein crystallization screen. Journal Applied Crystallography 42: 1035-42 (2009)

Molecular Dimensions MD1-106 MIDASplus

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MIDASplus™ is an updated version of the original MIDAS screen with new precipitants so let you explore chemical space even further. Developed in the laboratory of Dr Clemens Grimm at the University of Wurzburg, Germany.

MIDASplus™ 是原始 MIDAS 屏幕的更新版本，带有新的沉淀剂，让您进一步探索化学空间。 由德国维尔茨堡大学 Clemens Grimm 博士的实验室开发。

Examples of protein crystals grown using conditions from MIDASplus.

Features of MIDASplus™:

• Now includes the addition of PPGBA’s to increase the diversity of polymers.
• Ideal for both protein, protein/protein complexes, protein / nucleic acid complexes, and sensitive macromolecular complexes.
• Narrow range of pH and salt concentrations centered on physiological values.
• Every condition contains at least one alternative polymeric precipitant.
• Compatible with liquid handling robots.

MIDASplus™ is manufactured and distributed under an exclusive license with Dr. C. Grimm and Prof. Dr. U. Fischer.

References

Grimm, C., Chari, A., Reuter, K. & Fischer, U. (2010). Acta Cryst. D66, 685-697