分类目录归档：Hampton 蛋白结晶删选试剂-Crystallization Screens

Hampton GRAS 5 • GRAS 6 Screens

发表于由

Hampton GRAS 5 • GRAS 6 Screens

Applications

 GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary reagent, sampling pH 4.5 to 10

. Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.5 to 10; 8 unique buffers
Primary crystallization reagent:

GRAS 5: PEG 300, 400, MME 550, & 600
GRAS 6: PEG 1,000, MME 2,000, 3,350 & 4,000

 
Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description

GRAS Screen™ 5 and 6 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 5 and 6 has been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 5 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 5 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 5 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 6 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) at three concentrations versus eight unique buffers encompassing pH 4.5 to 10. GRAS Screen 6 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 6 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

Hampton GRAS 3 • GRAS 4 Screens

发表于由

Hampton GRAS 3 • GRAS 4 Screens

Applications

a.GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Salt is the primary, and Polyethylene glycol the secondary reagent, sampling pH 4-9

b.Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

Features

 Developed at Hampton Research

 Generally Recognized As Safe reagent formulation

 Samples pH 4.0 – 9.0 without an added buffer

 Primary crystallization reagent:

  24 unique Salts

  Secondary crystallization reagent:

  GRAS Screen 3: PEG 300, 400, MME 550, & 600

 GRAS Screen 4: PEG 1,000, MME 2,000, 3,350 & 4,000

 Compatible with Vapor diffusion, microbatch, free interface diffusion

 Reagents soluble between 4°C and 30°C

 Description
GRAS Screen™ 3 and GRAS Screen™ 4 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 3 and 4 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 3 samples twenty four salts, encompassing pH 4-9 versus four low molecular weight Polyethylene glycols (300, 400, MME 550, & 600). GRAS Screen 3 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 3 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 4 samples twenty four salts, encompassing pH 4-9 versus four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350 & 4,000). GRAS Screen 4 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 4 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-453 GRAS Screen 3 1 ml, Deep Well block format
HR2-454 GRAS Screen 4 1 ml, Deep Well block format

Hampton GRAS 1 • GRAS 2 Screens

发表于由

Hampton GRAS 1 • GRAS 2 Screens

Applications

GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9

Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens

 

Features

Generally Recognized As Safe reagent formulationDeveloped at Hampton Research

Samples pH 4.0 – 9.0 without an added buffer

Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600

GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000

Secondary crystallization reagent:

24 unique salts

Compatible with Vapor diffusion, microbatch, free interface diffusion

Reagents soluble between 4°C and 30°C

Antibody crystallization screen

Membrane protein crystallization screen

LCP compatible

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
HR2-452 GRAS Screen 2 1 ml, Deep Well block format

Hampton Pct™预结晶试验 PCT™ Pre-Crystallization Test

发表于由

Hampton Pct™预结晶试验 PCT™ Pre-Crystallization Test

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test

APPLICATIONS

  • Determine the appropriate sample concentration for crystallization screening

FEATURES

  • Conserve sample
  • Optimize sample concentration prior to initial screens
  • Provide insight to sample homogeneity

DESCRIPTION

The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.

The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.

Each PCT kit contains 4 unique reagents, 50 ml each. This kit is useful for researchers who already have crystallization plates and cover slides

Each PCT kit (with plates) contains 4 unique reagents, 30 ml each, plus 24 well VDX Plates with sealant and siliconized cover slides. This kit is useful for labs without access to crystallization plates and cover slides.

应用

  • 确定适宜的结晶筛选样品浓度

特征

  • 保存样本
  • 初始筛选前优化样品浓度
  • 提供样本同质性的洞察力

描述

采用PCT预结晶试验，确定适合结晶筛选的蛋白质浓度.样品浓度是一个重要的结晶变量。样品浓度过高会导致无定形沉淀，而样品过稀则会产生明显的落点。沉淀和澄清滴是典型的结晶筛选结果，在试剂条件下不促进结晶，是每个结晶筛的一部分。然而，通过优化屏幕上的蛋白质浓度，可以减少清晰和沉淀结果的数量，从而提高样品的利用率，同时增加结晶的机会。Pct可以最大限度地减少或防止屏幕导致大量沉淀或清除滴落的情况。

PCT试剂盒包含4种独特的、预先配制的、无菌过滤试剂，用于评估用于结晶筛选的蛋白质浓度。最初，样品蛋白与两种试剂混合，以确定蛋白质浓度是否适合结晶筛选。如果蛋白质对盐和聚合物浓度非常敏感，根据最初的PCT结果，可以使用第二组PCT试剂对该蛋白质进行评估。然后，pct结果将提供对适当样品浓度的洞察力，或表明其他诊断测试，如自然凝胶电泳或动态光散射，应用于证明适合结晶的样品均匀性。

每个PCT试剂盒包含4种独特的试剂，每个50毫升。这个工具包对于已经有晶化板和盖片的研究人员是有用的。

每个PCT试剂盒(带板)包含4种独特的试剂，每个30毫升，加上24孔VDX板，带有密封剂和硅化盖板。这个试剂盒是有用的实验室，没有接触结晶板和盖幻灯片。

HR2-140  PCT   50 ml bottles (4 ea), cover slides (1 pk)
HR2-142  PCT   (with plates) 30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)

RELATED ITEM(S)
Individual PCT Reagents

Hampton结晶用缓冲盐试剂盒– SaltRx

发表于由

Hampton结晶用缓冲盐试剂盒– SaltRx

Crystallization Screens > SaltRx 1 • SaltRx 2 > SaltRx 1 • SaltRx 2 • SaltRx HT

SaltRx 1 • SaltRx 2 • SaltRx HT

Applications

Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules

Features

Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
 Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
CAT NO NAME DESCRIPTION
HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format

RELATED ITEM(S)
Individual SaltRx1 • SaltRx2 • SaltRx HT Reagents

Hampton 乙二醇300 Polyethylene glycol 300

发表于由

Hampton 乙二醇300 Polyethylene glycol 300

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimize Reagents > Optimize – Polymers > Polyethylene glycol 300

APPLICATIONS

  • Crystallization grade Polyethylene glycol 300 for formulating screens or for optimization

DESCRIPTION

Polyethylene glycol 300

Synonyms: PEG 300
H(OCH2CH2)nOH
Mr 285-315
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24889419
Density 1.125 g/mL at 20°C

Measured Conductivity Range: 3.0 – 9.0 µS/cm at 25°C
Measured Refractive Index Range: 1.46450 – 1.46549 at 20°C

Peroxides (H2O2) ≤0.001%
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K  ≤0.02%
Li  ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.02%
Ni  ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

HR2-517 100% Polyethylene glycol 300 200 mL

RELATED ITEM(S)
PEGRx 1 • PEGRx 2 • PEGRx HT

Hampton Individual Solubility & Stability Reagents

发表于由

Hampton Individual Solubility & Stability Reagents

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Custom Shop Crystallization Reagents > Solubility & Stability > Individual Solubility & Stability Reagents

APPLICATIONS

  • Individual Solubility & Stability Screen reagents for screening, optimization and production

FEATURES

  • 1.0 ml volume in polypropylene screw cap tube.
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

HR2-072-**
** Refers to the reagent number in the kit.
For example, Solubility & Stability reagent number 1 = HR2-072-01

HR2-072-01 Solubility & Stability 01 (A01) 1.0 ml 100% Deionized water
HR2-072-02 Solubility & Stability 02 (A02) 1.0 ml 75% w/v Trichloroacetic acid
HR2-072-03 Solubility & Stability 03 (A03) 1.0 ml 250 mM L-Arginine
HR2-072-04 Solubility & Stability 04 (A04) 1.0 ml 250 mM L-Arginine, 250 mM L-Glutamic acid
HR2-072-05 Solubility & Stability 05 (A05) 1.0 ml 500 mM Glycine
HR2-072-06 Solubility & Stability 06 (A06) 1.0 ml 500 mM L-Proline
HR2-072-07 Solubility & Stability 07 (A07) 1.0 ml 120 mM L-Histidine
HR2-072-08 Solubility & Stability 08 (A08) 1.0 ml 500 mM ß-Alanine
HR2-072-09 Solubility & Stability 09 (A09) 1.0 ml 500 mM L-Serine
HR2-072-10 Solubility & Stability 10 (A10) 1.0 ml 500 mM L-Arginine ethyl ester dihydrochloride
HR2-072-11 Solubility & Stability 11 (A11) 1.0 ml 500 mM L-Argininamide dihydrochloride
HR2-072-12 Solubility & Stability 12 (A12) 1.0 ml 500 mM 6-Aminohexanoic acid
HR2-072-13 Solubility & Stability 13 (B01) 1.0 ml 500 mM Gly-gly
HR2-072-14 Solubility & Stability 14 (B02) 1.0 ml 200 mM Gly-gly-gly
HR2-072-15 Solubility & Stability 15 (B03) 1.0 ml 5% w/v Tryptone
HR2-072-16 Solubility & Stability 16 (B04) 1.0 ml 2,500 mM Betaine monohydrate
HR2-072-17 Solubility & Stability 17 (B05) 1.0 ml 750 mM D-(+)-Trehalose dihydrate
HR2-072-18 Solubility & Stability 18 (B06) 1.0 ml 2,000 mM Xylitol
HR2-072-19 Solubility & Stability 19 (B07) 1.0 ml 2,000 mM D-Sorbitol
HR2-072-20 Solubility & Stability 20 (B08) 1.0 ml 2,000 mM Sucrose
HR2-072-21 Solubility & Stability 21 (B09) 1.0 ml 500 mM Hydroxyectoine
HR2-072-22 Solubility & Stability 22 (B10) 1.0 ml 2,500 mM Trimethylamine N-oxide dihydrate
HR2-072-23 Solubility & Stability 23 (B11) 1.0 ml 2,000 mM Methyl-a-D-glucopyranoside
HR2-072-24 Solubility & Stability 24 (B12) 1.0 ml 10% v/v Triethylene glycol
HR2-072-25 Solubility & Stability 25 (C01) 1.0 ml 500 mM Spermine tetrahydrochloride
HR2-072-26 Solubility & Stability 26 (C02) 1.0 ml 500 mM Spermidine
HR2-072-27 Solubility & Stability 27 (C03) 1.0 ml 500 mM 5-Aminovaleric acid
HR2-072-28 Solubility & Stability 28 (C04) 1.0 ml 500 mM Glutaric acid
HR2-072-29 Solubility & Stability 29 (C05) 1.0 ml 80 mM Adipic acid
HR2-072-30 Solubility & Stability 30 (C06) 1.0 ml 500 mM Ethylenediamine dihydrochloride
HR2-072-31 Solubility & Stability 31 (C07) 1.0 ml 500 mM Guanidine hydrochloride
HR2-072-32 Solubility & Stability 32 (C08) 1.0 ml 500 mM Urea
HR2-072-33 Solubility & Stability 33 (C09) 1.0 ml 500 mM N-Methylurea
HR2-072-34 Solubility & Stability 34 (C10) 1.0 ml 200 mM N-Ethylurea
HR2-072-35 Solubility & Stability 35 (C11) 1.0 ml 30% w/v N-Methylformamide

Hampton 蛋白结晶删选试剂– Index HT

发表于由
Hampton 蛋白结晶删选试剂– Index HT

Applications

Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

多样结晶试剂系统删选试剂盒，用于蛋白质，复合物，肽，核酸，和水溶性的小分子。

Features

 Developed at Hampton Research

Screens classic, contemporary, & modern crystallization reagents

Samples pH 3 to 9

Compatible with microbatch, vapor diffusion, & liquid diffusion methods

Specially formulated reagent zones:

1 .Traditional salts versus pH

2.Neutralized organic acids

3.High [salt] with low [polymer]

4.High [polymer] with low [salt]

5.Low ionic strength versus pH

6.PEG & Salt versus pH

7.PEG & Salt

 Tube or Deep Well block format

Description
Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format.

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.

Index contains 96 unique reagents, 10 ml each.

Index HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C

Apo Kinase crystal. Initial hit from the Hampton Research Index Screen. Annie Hassell, Glaxo Smithkline.

Index • Index HT

Crystal of a heme-based sensor from Thermoanaerobacter Tengcongensis grown using the Hampton Research Index screen. Patricia Pellicena & John Kuriyan at University of California, Berkeley, USA.

CAT NO NAME DESCRIPTION
HR2-144 Index 10 ml, tube format
HR2-134 Index HT 1 ml, Deep Well block format

Hampton HEPES钠缓冲液 HEPES sodium Buffer

发表于由

Hampton HEPES钠缓冲液 HEPES sodium Buffer

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS  应用

  • Crystallization grade HEPES sodium buffer for formulating screens or for optimization

用于配制筛选或优化的结晶级 HEPES 钠缓冲液

FEATURES  特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

DESCRIPTION

HEPES sodium

Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C

HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

HR2-577 1.0 M HEPES sodium 100 mL
HR2-733 1.0 M HEPES sodium pH 7.5 100 mL

RELATED ITEM(S)  相关产品

REFERENCES 参考文献

1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.