分类目录归档：Hampton

Hampton HR4-217晶体探针 Crystal Probe

发表于由

Hampton HR4-217晶体探针 Crystal Probe

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Tools & Seeding > Crystal Probe™ > Crystal Probe

晶体探针

应用

  • 晶体操纵
  • 播种

特征

  • 带塑料柄的不锈钢探头

描述

一次性水晶探针机械手由0.12毫米x 30毫米不锈钢制成，尖端探头连接在20毫米塑料手柄上。用于分割叶片团簇和一般晶体操作。小尺寸和一次性的格式，使他们方便的旅行到同步加速器。

Crystal Probe

APPLICATIONS

  • Crystal manipulation
  • Seeding

FEATURES

  • Stainless steel probe with plastic handle

DESCRIPTION

Disposable Crystal Probe manipulators made from a 0.12 mm x 30 mm stainless steel, pointed-end probe attached to a 20 mm plastic handle. Useful for breaking apart blade clusters and general crystal manipulation. Small size and disposable format makes them convenient for trips to the synchrotron.

HR4-217 Crystal Probe 12 pack

Hampton HR3-172 VDX 24孔板，带密封剂 VDX Plate, with sealant

发表于由

Hampton HR3-172VDX 24孔板，带密封剂 VDX Plate, with sealant

APPLICATIONS

  • Hanging drop crystallization
  • Sitting drop crystallization with Micro-Bridges®
  • Dialysis crystallization with Dialysis Buttons™

FEATURES

  • 24 well plate with raised cover
  • Sealant applied to 24 wells, ready to seal with cover slides
  • Compatible with 22 mm diameter square and circle cover slides
  • Optically clear plastic
  • Extremely versatile and cost-effective crystallization platform
  • Compatible with UV crystal imaging when used with HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides and HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

DESCRIPTION

24 well crystallization plate for hanging drop or sitting drop vapor diffusion crystallization (when used with Micro-Bridges) or dialysis crystallization (when used with Dialysis Buttons). Stackable, optically clear plastic 24 well plates with raised covers (to allow room for cover slides) and flat bottoms for exceptional optics. Raised, wide rings around each reservoir (well) minimize cross contamination and allow each well to be individually sealed with 22 mm diameter circle or square cover slides. Plates are individually wrapped and supplied with applied sealant.

15.0 cm x 10.8 cm plate footprint is convenient for manual pipetting and well access while at the same time compatible with some automated liquid handling systems.
Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm.
Approximate well size: 1.7 cm x 1.6 cm.
Typical well volume: 500 to 1,000 µl.
Well capacity: 3.5 ml.

HR3-172 VDX™ Plate with sealant 24 plate case
HR3-171 VDX™ Plate with sealant 40 plate case
HR3-170 VDX™ Plate with sealant 100 plate case

RELATED ITEM(S)     相关产品：

22 mm Siliconized Glass Cover Slides

  • 22 mm硅化玻璃盖板滑片

OptiClear Plastic Cover Slides

应用

  • 悬滴结晶
  • 微桥坐滴结晶
  • 透析按钮™的透析结晶

特征

  • 24井板凸起盖
  • 密封胶适用于24口井，准备用盖板密封。
  • 适用于直径22 mm的方形和圆形盖板滑片。
  • 光学透明塑料
  • 用途广、成本效益高的结晶平台
  • 适用于HR3-231和HR3-233-22 mm x 0.22 mm硅化圆盖幻灯片和HR3-215和HR3-217-22 mm x 0.22 mm硅化方形覆盖片。

描述

24井结晶板，用于悬滴或坐滴蒸汽扩散结晶(用于微桥)或透析结晶(当使用与透析按钮)。可堆叠，光学清晰的塑料24井板与凸起的盖(以允许空间盖幻灯片)和平底为特殊的光学。提高，宽环周围的每个储油层(井)尽量减少交叉污染，并允许每口井分别密封22毫米直径圆或方盖幻灯片。盘子是单独包装和提供应用密封胶。

15.0cmx10.8cm板足迹便于人工打管和良好的存取，同时与一些自动液体处理系统兼容。
近似尺寸：15.0厘米x 10.6厘米x2.2厘米。
近似井大小：1.7厘米x1.6厘米。
典型井容量：500~1000升。
容量：3.5毫升。

Hampton Mounted CryoLoop – 20 micron with EasySnap MicroTube

发表于由

Hampton Mounted CryoLoop – 20 micron with EasySnap MicroTube

Applications
Cryocrystallography
Features
Synthetic CryoLoop on stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
Complete range of loop diameters from 0.025 – 1.0 mm
Compatible with all CrystalCap Systems
Description 
Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop. 

18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic

CryoLoop properly installed in a CrystalCap Magnetic

Hampton Mounted CryoLoop – 20 micron with EasySnap MicroTube

CAT NO NAME DESCRIPTION
HR4-313 Mounted CryoLoop – 20 micron 0.025 – 0.05 mm – 25 pack
HR4-625 Mounted CryoLoop – 20 micron 0.05 – 0.1 mm – 25 pack
HR4-955 Mounted CryoLoop – 20 micron 0.1 – 0.2 mm – 25 pack
HR4-957 Mounted CryoLoop – 20 micron 0.2 – 0.3 mm – 25 pack
HR4-959 Mounted CryoLoop – 20 micron 0.3 – 0.4 mm – 25 pack
HR4-961 Mounted CryoLoop – 20 micron 0.4 – 0.5 mm – 25 pack
HR4-963 Mounted CryoLoop – 20 micron 0.5 – 0.7 mm – 25 pack
HR4-965 Mounted CryoLoop – 20 micron 0.7 – 1.0 mm – 25 pack
HR4-953 Mounted CryoLoop – 20 micron Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

References  参考文献

1.FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: Structure, mechanism, and acyl carrier protein binding. Ryan M. Van Wagoner and Jon Clardy. Structure, 2006, Volume 14, Number 9, 1425-1435.

Hampton 铝盒CryoCane

发表于由

Hampton 铝盒CryoCane

Applications
Organized storage of CrystalCaps 有序的存放结晶帽

Features
5 vial holding cane
Made of aluminum
Compatible with the CrystalCap systems
Description
Aluminum CryoCane firmly hold CrystalCap™ vials for storage in dewar-type, liquid nitrogen freezers. The 5 vial CryoCane offers base tabs for proper CrystalCap seating. Length of 5 vial version: 11 5/16” (28.7 cm).

HR4-711 (front – discontinued) HR4-709 (back)

Hampton 铝盒CryoCane

CAT NO NAME DESCRIPTION
HR4-709 CryoCane 5 Vial Holder 12 pack

Hampton 蛋白质结晶试剂和耗材介绍

特色

发表于由

Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域受信赖的品牌之一。

上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Hampton research主要产品：

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类，聚合物，有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板（悬滴法和坐滴法，微量透析，微配液，自由界面扩散，凝胶以及无容器悬浮法）及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop

Crystallization Reagents

 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类，缓冲液和冷冻液的简便型试剂盒
Tools，Seeding&Resin 结晶用探针，结晶笔，seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商之一，该公司的产品目录是由上海金畔生物科技有限公司提供，该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类：Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test（PCT预结晶试验）

应用：结晶筛选，确定相应的样品浓度，PCT试验用来预结晶结晶筛选，以确定适当的蛋白质浓度

  1. Slice pH（PH切片）

应用：溶解度和结晶筛选

  1. Solubility & Stability Screen（溶解度和稳定性的屏幕）

应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。

  1. Index

应用：不同的试剂系统的结晶屏幕的蛋白质复合物，多肽，核酸，水溶性小分子。

  1. Crystal Screen（晶体屏幕）

应用：

主屏幕的可溶性肽，蛋白质，核酸，和水溶性小分子。稀疏矩阵的加法屏幕

5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质，复合物，缩氨酸，核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质，缩氨酸，核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质，缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质，缩氨酸，核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶，分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂，与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体，单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化

16.Natrix?用于核酸，蛋白核酸聚合物的结晶

17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂，用于区分蛋白晶体和盐晶体

第二类：Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类：Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体

2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类

5.Optimize-Buffers缓冲液

6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物

8.Optimize-Cryoprotectants

9.Optimize-Oils用于结晶的油类

10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类：StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类：Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶

8.Micro-Bridges?微桥-坐滴结晶的支撑物

9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂，油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类：Tools, Seeding & Resin（结晶工具、树脂）

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool

第七类：Cryocrystallography

  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类：Capillary Mounts & Supplies（毛细管座椅支架供应）

第九类：Goniometer Heads & Supplies（测角头及用品）

第十类：Xenon Derivatization（氙气衍生物）

第十一类：Labels & Pens（标签和记录笔）

第十二类：Protein Crystallization Standards（蛋白质结晶的标准）

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。

Hampton代理，欢迎访问Hampton官网或者咨询我们获取更多相关产品信息。

上海金畔生物科技有限公司（www.jinpanbio.cn）提供生命科学研究领域系列产品，包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；WAKO日本和光纯药、修饰性PEG（Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、小鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。

Hampton research代理 HR8-133 Seeding Tool

发表于由

Hampton research代理 HR8-133 Seeding Tool

Seeding Tool

APPLICATIONS

  • Streak Seeding

FEATURES

  • Natural fiber
  • Built-in handle/cover

DESCRIPTION

During streak seeding, one touches the Seeding Tool to crystalline material to dislodge, remove and transfer small crystals (seeds) to a drop that will support the growth of potentially larger and more perfect crystals.

A seed can provide a template on which additional macromolecules can assemble and under the proper conditions, grow to form a large single crystal. Using seeding can avoid problems associated with growing crystals from spontaneous nucleation. Seeds can grow into larger crystals in the metastable region of the solubility curve, which is a region of lower, relative supersaturation. One can also streak seed from phase separation or amorphous material as a diagnostic to confirm whether the material is crystalline.

The Seeding Tool is a 1 cm natural fiber attached to a stainless steel pin and plastic handle. The Seeding Tool is supplied with a cover to protect the fiber and when the cover is placed on the back side of the Seeding Tool, it makes an excellent handle.

播种工具

应用

  • 条播

特征

  • 天然纤维
  • 内置手柄/盖子

描述

在条纹播种过程中，一个人将播种工具接触到水晶材料上，将小晶体(种子)移走，并将其转移到一滴将支持潜在更大、更完美晶体生长的滴中。

种子可以提供一个模板，使更多的大分子可以在适当的条件下聚集和生长，形成一个大的单晶。使用种子可以避免与自发成核晶体生长有关的问题。种子在溶解度曲线的亚稳区可以生长成较大的晶体，这是一个较低、相对过饱和的区域。还可以从相分离或非晶态材料中剥离种子，以确定该材料是否为晶态材料。

播种工具是一个1厘米的天然纤维连接到不锈钢销和塑料手柄。所述播种工具具有保护纤维的罩，当所述覆盖被放置在所述播种工具的背面时，使其具有极好的手柄。

HR8-133 Seeding Tool 5 pack

REFERENCES

1. Seeds to crystals. Terese Bergfors. Journal of Structrual Biology 142 (2003) 66-76.

2. Applications of the streak seeding technique in protein crystallization. Stura and Wilson. Journal of Crystal Growth, 110 (1991) 270-282. 110 (1991) 270-282.

3. Applications of the streak seeding technique in protein crystallization. Stura and Wilson. Journal of CrystaL Growth, 110 (1991) 270-282.

4. Crystallization of pig mitochondrial aspartate aminotransferase by seeding with crystals of the chicken mitochondrial isoenzyme. Eichele G, Ford GC, Jansonius JN. J Mol Biol. 1979 Dec 5;135(2):513-6. PubMed PMID: 537086.

Hampton research代理 HR2-801 TCEP hydrochloride

发表于由

Hampton research代理 HR2-801 TCEP hydrochloride

TCEP hydrochloride

APPLICATIONS

  • Stable reducing agent for crystallization trials

FEATURES

  • Highly soluble
  • Odorless and non-volatile
  • Effective at acidic and alkaline pH

DESCRIPTION

TCEP hydrochloride is Tri(2-carboxyethyl)phosphine hydro-chloride.

TCEP hydrochloride is an odorless, non-volatile reducing agent that is more stable and effective than DTT or 2-Mercaptoethanol. Unlike DTT, TCEP hydrochloride retains its reducing power at acid pHs (pH 5) and at pHs above 7.5 and is stable in air. TCEP hydrochloride is soluble in water to 310 grams per liter. For most applications, 5-50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature. TCEP hydrochloride is unreactive toward other functional groups found in proteins. Unlike DTT (dithiothreitol), TCEP hydrochloride does not contain a free thiol, and therefore does not require removal before reaction with thiol-reactive reagents. TCEP reduces disulfides but apparently not mercury-thiol bonds. TCEP hydrochloride is compatible with many heavy atoms and may be used during heavy atom derivatization. TCEP hydrochloride is more stable at a higher pH and at higher temperatures than is DTT and for a longer period of time in buffers without metal chelators such as EGTA. With TCEP, removal of the reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, protein crystallization).

TCEP is typically very soluble in aqueous buffers at nearly any pH. Therefore, working concentrations and 10X stock solutions may be readily prepared in most aqueous buffers. TCEP is stable in aqueous, acidic, and basic solutions. When TCEP is dissolved directly in water, the resulting pH is approximately 2.5. TCEP is not very stable in phosphate buffers, especially at neutral pH. Therefore, if TCEP is to be used in PBS buffers, prepare the working solution immediately before use. TCEP may be used as a substitute loading buffer for SDS-PAGE; use a final concentration of 50 mM TCEP. Since TCEP is charged in solution, it is not compatible for use in isoelectric focusing.

A 10 mM TCEP hydrochloride concentration can reduce the pH of a 100 mM buffer by approximately 0.5 pH units; 1 mM TCEP hydrochloride concentration reducing the pH of a 100 mM buffer by 0.05 pH units.

Stability in Solution
TCEP is stable in aqueous acidic and basic solutions. When TCEP is dissolved directly in water the resulting pH is 2.5. Various buffers may be used for the reductions. Studies indicate that no change in concentration occurs after 24 hour incubation at room temperature in 100 mM HCL, 100 mM NaOH, or any of the following 50 mM buffers Tris HCl (pH 7.5, 8.5, 9.5), HEPES(pH 6.8, 8.2), Borate(pH 8.2, 10.2), and CAPS (pH 9.7, 11.1). Even after weeks in these buffers, less than 20% of the TCEP was oxidized.

TCEP is not particularly stable in phosphate buffers, especially at neutral pH. Experiments indicate that TCEP completely oxidizes within 72 hours in 0.35M phosphate-buffered saline (PBS), pH 7.0. Approximately 50% oxidation occurs in the same amount of time in 0.15M PBS, pH 8.0. Only minimal oxidation occurs in PBS at pH > 10.5 or < 6.0. Therefore, if TCEP is to be used in PBS buffers, prepare the working solution immediately before use.

Solid TCEP hydrochloride is supplied packaged in argon to minimize oxidation.

Molecular formula C9H15O6P • HCl
Molecular weight 286.65
CAS [51805-45-9]
Beilstein Registry Number 3724376
Purity ≥99.0%

Stable at room temperature for up to 2 years after receipt.

TCEP盐酸盐

应用

  • 结晶试验用稳定还原剂

特征

  • 高可溶性
  • 无臭非挥发性
  • 在酸性和碱性pH下有效

描述

盐酸TCEP为三(2-羧乙基)膦盐酸盐.

盐酸TCEP是一种无臭、非挥发性的还原剂，比DTT或2-巯基乙醇更稳定有效.与DTT不同，盐酸TCEP在酸性PHS(PH 5)和PHS高于7.5时仍保持其还原能力，且在空气中稳定。盐酸TCEP可溶于水至每升310克。在大多数应用中，5-50毫米TCEP提供了足够的摩尔过量，可以在室温下几分钟内有效地减少肽或蛋白质二硫键。盐酸TCEP对蛋白质中的其他官能团没有反应。与DTT(二硫苏糖醇)不同，TCEP盐酸盐不含游离硫醇，因此在与硫醇反应试剂反应前不需要去除。TCEP减少了二硫键，但明显没有汞-硫醇键。盐酸TCEP与许多重原子相容，可用于重原子衍生。与DTT相比，盐酸TCEP在较高的pH值和较高的温度下更稳定，且在没有EGTA等金属螯合剂的缓冲液中具有更长的稳定性。使用TCEP时，在大多数应用之前不需要去除还原剂(例如组氨酸标记的蛋白质纯化、蛋白质结晶)。

TCEP通常在几乎任何pH值下都能溶于水缓冲液中。因此，工作浓度和10X储存溶液可以很容易地在大多数水缓冲器中制备。TCEP在水溶液、酸性和碱性溶液中是稳定的。当TCEP直接溶于水中时，其pH值约为2.5。TCEP在磷酸盐缓冲液中不太稳定，特别是在中性pH条件下。因此，如果要在PBS缓冲器中使用TCEP，请在使用前立即准备工作溶液。TCEP可作为SDS-PAGE的替代加载缓冲液；最终浓度为50 mm TCEP。由于TCEP是在溶液中充电的，所以它不适合用于等电聚焦。

10 mmTCEP盐酸盐浓度可使100 mm缓冲液的pH降低约0.5个pH单位；1mm TCEP盐酸TCEP浓度可使100 mm缓冲液的pH降低0.05个pH单位。

溶液稳定性
TCEP在酸性和碱性水溶液中是稳定的。当TCEP直接溶于水中时，其pH值为2.5。各种缓冲器可用于减缩。研究表明，在100 mm HCL、100 mm NaOH或以下任何50 mm缓冲介质Tris HCl(pH 7.5、8.5、9.5)、HEPES(pH 6.8、8.2)、硼酸盐(pH 8.2、10.2)和CAPS(pH 9.7，11.1)中，室温孵育24小时后，浓度不会发生变化。即使在这些缓冲的几个星期后，只有不到20%的TCEP被氧化。

TCEP在磷酸盐缓冲液中不特别稳定，特别是在中性pH条件下。实验表明，在pH7.0的0.35m磷酸盐缓冲溶液(PBS)中，TCEP在72h内完全氧化。约50%的氧化发生在相同的时间内，在0.15mPBS，pH8.0。PH>10.5或<6.0时，PBS只发生极少量的氧化。因此，如果要在PBS缓冲器中使用TCEP，请在使用前立即准备工作溶液。

固体TCEP盐酸盐被包装在氩气中，以减少氧化。

分子式C9H15O6P·HCl
分子量286.65
核证机关[51805-45-9]
伯尔斯坦登记处编号3724376
纯度≥99.0%

在室温下稳定，在收到后长达2年。

HR2-651 TCEP hydrochloride 1 gram
HR2-801 TCEP hydrochloride 10 grams

Hampton research代理 HR2-128 Crystal Screen Lite

发表于由

Hampton research代理 HR2-128 Crystal Screen Lite

MembFac • Crystal Screen Lite • MembFac HT

APPLICATIONS

  • Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility

FEATURES

  • Membrane protein sparse matrix screens
  • Sparse matrix formula efficiently samples salts, polymers, organics and pH
  • pH range 4.6 – 8.5
  • Formulated for use with detergents
  • Tube or Deep Well block format
  • Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

DESCRIPTION

MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins.

MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2 Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format

Hampton research HR2-213 Grid Screen PEG 6000

发表于由

Hampton research HR2-213 Grid Screen PEG 6000

APPLICATIONS

  • Primary or secondary, Polyethylene glycol 6,000 versus pH grid crystallization screen for biological macromolecules

FEATURES

  • A systematic grid screen varying Polyethylene glycol 6,000 concentration versus pH
  • Samples pH 4 to 9
  • Samples four concentrations of Polyethylene glycol 6,000
  • Precise pH versus Polyethylene glycol 6,000 screen
    • pH adjusted after salt addition; 25°Celsius
  • Combine reagents between rows or columns to create expanded grid screens

DESCRIPTION

Grid Screen™ PEG 6000 is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Polyethylene glycol 6,000 between pH 4.0 and 9.0.

Grid Screen PEG 6000 evaluates Polyethylene glycol at four concentrations (5, 10, 20, 30 %w/v) versus six pH levels (4, 5, 6, 7, 8, 9).

Grid Screen PEG 6000 consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity polymer and buffers. Individual reagents are available through the Hampton Research Custom Shop.

HR2-213 Grid Screen PEG 6000 10 ml, tube format

Hampton research HR2-211 Grid Screen Ammonium Sulfate

发表于由

Hampton research HR2-211 Grid Screen Ammonium Sulfate

APPLICATIONS

  • Primary or secondary, Ammonium sulfate versus pH grid crystallization screen for biological macromolecules

FEATURES

  • A systematic grid screen varying Ammonium sulfate concentration versus pH
  • Samples pH 4 to 9
  • Samples four concentrations of Ammonium sulfate
  • Precise pH versus Ammonium sulfate screen
    • pH adjusted after salt addition; 25°Celsius
  • Combine reagents between rows or columns to create expanded grid screens

DESCRIPTION

Grid Screen™ Ammonium Sulfate is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Ammonium sulfate between pH 4.0 and 9.0. Grid Screen Ammonium Sulfate systematically evaluates Ammonium sulfate at four concentrations (0.8, 1.6, 2.4, 3.0 M) versus 6 pH levels (4, 5, 6, 7, 8, 9).

Grid Screen Ammonium sulfate consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salt and buffers. Individual reagents are available through the Hampton Research Custom Shop.

HR2-211 Grid Screen Ammonium Sulfate 10 ml, tube format