标签归档：Hampton 蛋白质结晶试剂盒

Hampton GRAS 1 • GRAS 2 Screens

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Hampton GRAS 1 • GRAS 2 Screens

Applications

GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9

Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens

 

Features

Generally Recognized As Safe reagent formulationDeveloped at Hampton Research

Samples pH 4.0 – 9.0 without an added buffer

Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600

GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000

Secondary crystallization reagent:

24 unique salts

Compatible with Vapor diffusion, microbatch, free interface diffusion

Reagents soluble between 4°C and 30°C

Antibody crystallization screen

Membrane protein crystallization screen

LCP compatible

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
HR2-452 GRAS Screen 2 1 ml, Deep Well block format

Hampton 透析按钮 Dialysis Buttons

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Hampton 透析按钮 Dialysis Buttons

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

Hampton 透析按钮 Dialysis Buttons

Applications
Crystallization by dialysis, protein folding, & small volume sample dialysis

Features
Low volume dialysis
Fits in 24 well plates
Use over and over again

Methodologies
Controlled crystal dehydration.4

Description 
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3 Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

应用

透析结晶，蛋白质折叠，小容量样品透析

特征

低容量透析
适用于24孔板
一遍又一遍地使用

方法

控制晶体脱水
描述 
在透析法中，样品被半透膜从“沉淀剂”中分离出来，这种膜允许小的试剂分子通过，但防止生物大分子穿过膜。1-3透析按钮是注射成型的聚苯乙烯，大小与一个小按钮。样品被放置在这个房间，以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部，并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米，外径约为10毫米而没有O形环，以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米，外径约为15毫米，没有O形环，而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头，以协助将透析膜应用到按钮上，并与所提供的o-环固定在一起。

Hampton 透析按钮 Dialysis Buttons

CAT NO NAME DESCRIPTION
HR3-336 Dialysis Buttons Sampler 5 of each size listed below
HR3-314 5 µl Dialysis Button 50 pack
HR3-316 10 µl Dialysis Button 50 pack
HR3-318 15 µl Dialysis Button 50 pack
HR3-320 20 µl Dialysis Button 50 pack
HR3-326 50 µl Dialysis Button 50 pack
HR3-328 100 µl Dialysis Button 50 pack
HR3-330 200 µl Dialysis Button 50 pack
HR3-332 350 µl Dialysis Button 50 pack

1 Related Item  相关产品信息
Applicator for Dialysis Buttons

References  参考文献
1.McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2.Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3.Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4.Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

Hampton research Additive Screen • Additive Screen HT

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Hampton research Additive Screen • Additive Screen HT

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > Additive Screen > Additive Screen • Additive Screen HT

APPLICATIONS

  • Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

FEATURES

  • 18 classes of reagents
  • Highly concentrated (10x) reagent formulation
  • 96 unique reagents and excipients
  • Tube or Deep Well block format
  • Crystallization or sample solubility optimization

DESCRIPTION

Additive Screen is a library of reagents that can affect the solubility and crystallization of biological macromolecules, including both soluble and membrane proteins.

These reagents can perturb and manipulate sample-sample and sample-solvent interactions, as well as perturb water structure which can alter and improve both the solubility and crystallization of a sample. Additives can stabilize or engender conformity by specific interaction with the macromolecules. There are numerous reports of the use of additives to improve the quality and size of macromolecular crystals.1-5

Additive screen reagent classifications include multivalent, salt, amino acid, dissociating agent, linker, polyamine, osmolyte, chaotrope, co-factor, reducing agent, chelating agent, polymer, carbohydrate, non-detergent, amphiphile, detergent, non-volatile organic and volatile organic.

Additive Screen contains 96 unique reagents, 1 ml each.

Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block.

添加剂筛·添加剂筛HT

应用

  • 操纵样品-样品和样品-溶剂的相互作用，以改善晶体或改变样品的溶解度。

特征

  • 18类试剂
  • 高浓度(10倍)试剂配方
  • 96种独特试剂和辅料
  • 管式或深井块格式
  • 结晶或样品溶解度优化

描述

添加剂筛选是影响生物大分子(包括可溶性蛋白质和膜蛋白)溶解度和结晶性的试剂库。

这些试剂可以干扰和操纵样品-样品和样品-溶剂的相互作用，也可以改变和改善样品的溶解度和结晶性的扰动水结构。添加剂可以通过与大分子的特定相互作用来稳定或产生整合。有许多关于使用添加剂来提高大分子晶体质量和尺寸的报道。1-5

添加剂筛选试剂可分为多价、盐、氨基酸、解离剂、连接剂、多胺、渗透压剂、壳聚糖、辅助因子、还原剂、螯合剂、聚合物、碳水化合物、非洗涤剂、两亲性试剂、洗涤剂、非挥发性有机和挥发性有机化合物.

添加剂筛选包含96个独特的试剂，每个1毫升。

添加剂筛选HT包含96个独特的试剂，每个1毫升，在一个单一的深井区块。

HR2-428 Additive Screen 1 ml, tube format
HR2-138 Additive Screen HT 1 ml, Deep Well block format

RELATED ITEM(S)
Individual Additive Screen Reagents

Hampton research HR2-245 StockOptions Salt

发表于由

Hampton research HR2-245 StockOptions Salt

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimization Screens > StockOptions Salt > StockOptions Salt

应用领域

  • 用于筛分配方和优化的结晶级盐试剂原液

特征

  • 预先配制和无菌过滤
  • 从筛选到优化的轻松过渡
  • 与Hampton Research筛选，试剂盒和试剂协同作用
  • 49种独特的盐，包括有机酸
  • 高度浓缩，随时可以稀释

描述

StockOptions试剂盒是用于大分子晶体种植者的试剂工具箱。他们以方便，经济的试剂盒提供精确配制的高质量结晶级试剂储备。这些试剂盒中使用的化学药品是汉普顿研究试剂盒中使用的相同结晶度，超高纯度化学药品，例如Crystal Screen™和Crystal Screen 2™。 StockOptions试剂在严格的质量标准下精心配制，以确保可靠的性能和批次间的一致性。

StockOptions Salt

APPLICATIONS

  • Crystallization grade salt reagent stock solutions for screen formulation and optimization

FEATURES

  • Preformulated and sterile filtered
  • Easy transition from screening to optimization
  • Synergistic with Hampton Research screens, kits, & reagents
  • 49 unique salts, including organic acids
  • Highly concentrated, ready to dilute

DESCRIPTION

StockOptions reagent kits are reagent tool boxes for the macromolecular crystal grower. They offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in these kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits such as Crystal Screen™ and Crystal Screen 2™. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

HR2-245 StockOptions Salt Kit 10 ml, tube format

Hampton 蛋白结晶96孔板Swissci-MRC 2 Well UVP

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Hampton 蛋白结晶96孔板Swissci-MRC 2 Well UVP

Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。

APPLICATIONS

  • Sitting drop crystallization

应用：
坐滴结晶

FEATURES

  • Two drop wells per reservoir
  • UV compatible (UVP)
  • Standard ANSI/SLAS 1-2004 form for automation (SBS)
  • Drop volume: 100 nl to 5 µl
  • Lens effect drop wells for enhanced optics
  • Reservoir volume: 50 to 100 µl
  • Micro-numbering alongside drop volumes
  • Rigid plate structure
  • Drop well allows easier crystal harvesting
  • Wide partition walls between wells improve sealing

特征：
每个水库两口井
紫外线兼容 (UVP)
用于自动化 (SBS) 的标准 ANSI/SLAS 1-2004 表格
液滴体积：100 nl 至 5 µl
用于增强光学性能的镜头效果下降井
水库体积：50 至 100 µl
与液滴体积一起进行微编号
刚性板结构
下降井允许更容易的晶体收获
井之间的宽隔墙提高了密封性

DESCRIPTION

The MRC 2 Well Crystallization plate manufactured by Swissci is a 2 drop chamber, 96 well crystallization plate for sitting drop vapor diffusion. Molded from a UVP polymer, the plate is UV compatible.

The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, United Kingdom) in collaboration with Dr. Jan Löwe. Designed to meet the stringent requirements specified by crystallographers, and made of proprietary new materials that facilitate enhanced imaging, the MRC Crystallization Plate provides easier access and removal of crystals, improved mixing of reagent and sample, and reduced sample and reagent volumes.

Easy Crystal Retrieval Raised wide wells make the crystal mounting especially easy.

Easy Viewing The wells are a wide conical shape and have a lens effect for perfect illumination. The micro-numbering ensures you will never get lost again when using the microscope. The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.

Better Sealing Wide partition walls between the wells give plenty of area for sealing with tape or film. No central bending occurs in this very robust structure.

Wide Range of Volumes Typical volumes are 50 to 100 µl of reservoir and 100 nl to 5 µl drop size. The 192 optical wells offer twice the number of experiments of experimental constructions.

SBS Standard The plate is designed to the 96 well SBS standard for all common holders and external alphanumeric identification. The MRC Crystallization Plate is suitable for centrifugation.

The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

描述
Swissci 制造的 MRC 2 孔结晶板是一个 2 滴室、96 孔结晶板，用于坐滴蒸汽扩散。该板由 UVP 聚合物模制而成，与 UV 兼容。

该板是在 MRC 分子生物学实验室（英国剑桥）与 Jan Löwe 博士合作开发的。 MRC 结晶板专为满足晶体学家指定的严格要求而设计，由促进增强成像的专有新材料制成，可更轻松地接触和去除晶体，改善试剂和样品的混合，并减少样品和试剂体积。

轻松取回水晶 凸起的宽孔使水晶安装特别容易。

易于观察 孔为宽锥形，具有透镜效果，可实现完美照明。微编号确保您在使用显微镜时不会再迷路。光学优异的聚合物可透过紫外线，可用于区分盐和蛋白质晶体。

更好的密封 孔之间的宽隔墙为用胶带或薄膜密封提供了足够的空间。在这种非常坚固的结构中不会发生中心弯曲。

广泛的体积范围 典型的体积是 50 到 100 µl 的容器和 100 nl 到 5 µl 的液滴大小。 192 个光学井提供了两倍于实验结构的实验数量。

SBS 标准板设计符合 96 孔 SBS 标准，适用于所有常见支架和外部字母数字识别。 MRC 结晶板适用于离心。

可以使用 Crystal Clear Sealing Film (HR3-609)、3 英寸宽的 Crystal Clear Sealing Tape (HR4-506) 或 ClearSeal Film™ (HR4-521) 密封板。

HR3-082/HR3-083 MRC 2 Well Crystallization Plate
HR3-082 MRC 2 Well Crystallization Plate 10 plate case
HR3-083 MRC 2 Well Crystallization Plate 40 plate case

RELATED ITEM(S)

Hampton Applicator for Dialysis Buttons

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Hampton Applicator for Dialysis Buttons

Applications
Application of dialysis membrane to Dialysis Buttons
Features
Manufactured of glass
Small applicator – fits 5 to 100 µl Dialysis Buttons

Description
The Applicator makes easier work of applying the dialysis membrane and O-ring to Dialysis Buttons. It positions and holds the dialysis membrane on the buttons and also allows the easy application and position of the O-ring to secure the dialysis membrane onto the Dialysis Button. The Applicator for Dialysis Buttons is manufactured of glass and is available in one size. The Small Applicator (HR4-348) is designed for use with 5 to 100 µl Dialysis Buttons.

应用
透析膜在透析按钮中的应用
特征
玻璃制
小喷头-适合5至100升透析按钮
描述 
该应用程序使透析膜和O环更容易地应用到透析按钮上.它在按钮上放置和保持透析膜，并允许O环的简单应用和位置将透析膜固定在透析按钮上。透析按钮的应用程序是由玻璃制造的，只有一种尺寸。该小型应用程序(HR4-348)是专为使用5至100升透析按钮.

Applicator for Dialysis Buttons

Dialysis Crystallization

Applicator for Dialysis Button

Using the applicator for Dialysis Buttons    透析按钮应用程序

CAT NO NAME DESCRIPTION
HR4-348 Small Applicator use with small Dialysis Buttons (5-100 ul)

1 Related Item   相关产品：
Dialysis Buttons