标签归档：Hampton 蛋白质结晶试剂盒

Hampton CrystalEX Second Generation (Corning)

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Hampton CrystalEX Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton Solubility & Stability Screen

发表于由
Hampton Solubility & Stability Screen

Solubility & Stability Screen（溶解度和稳定性的屏幕）

应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。

Solubility & Stability Screen

Solubility & Stability Screen HR2-072

Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features

Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

Hampton 银弹 Silver Bullets

发表于由

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 
Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton Tacsimate pH 4, 5, 6, 7, 8, & 9试剂

发表于由

Hampton Tacsimate pH 4, 5, 6, 7, 8, & 9试剂

APPLICATIONS

  • Crystallization grade Tacsimate for formulating screens or for optimization

FEATURES

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

DESCRIPTION

To screen pH as a crystallization variable using Tacsimate one can mix together different ratios of different pH Tacsimate solutions. Refer to the pdf “Mixing Tacsimate Stocks to Screen pH” on this page. Or, one can add a buffer to the crystallizaiton reagent containing Tacsimate. Recommended final buffer concentration in the reagent well is 0.05 to 0.1 M.

Tacsimate
A pH titrated mixture of organic acids. 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic. pH adjusted using NaOH

Measured pH of 100% Tacsimate is 4.0 at 25°C
Measured pH of 100% Tacsimate is 5.0 at 25°C
Measured pH of 100% Tacsimate is 6.0 at 25°C
Measured pH of 100% Tacsimate is 7.0 at 25°C
Measured pH of 100% Tacsimate is 8.0 at 25°C
Measured pH of 100% Tacsimate is 9.0 at 25°C

CAT NO NAME DESCRIPTION
HR2-823 100% Tacsimate pH 4.0 200 mL
HR2-825 100% Tacsimate pH 5.0 200 mL
HR2-827 100% Tacsimate pH 6.0 200 mL
HR2-755 100% Tacsimate pH 7.0 200 mL
HR2-829 100% Tacsimate pH 8.0 200 mL
HR2-813 100% Tacsimate pH 9.0 200 mL

Hampton 洗涤试剂Detergent Screen

发表于由

Hampton 洗涤试剂Detergent Screen

Detergent Screen
96 detergent formulations for sample and crystal optimization

Detergent Screen

A tetragonal crystal of AppA, a photoreceptor from Rhodobacter sphaeroides, grown in Hampton Research Detergent Screen. Vladimira Dragnea, Indiana University.

Crystal of beta-lactamase grown in the presence of detergent. James Knox, University of Connecticut

Applications   应用

Manipulate sample-sample and sample-solvent interactions, including non-specific aggregation due to hydrophobic interactions, to alter sample solubility, or obtain or improve crystalline samples.
操纵样品-样品和样品-溶剂的相互作用，包括由于疏水相互作用而产生的非特异性聚集，以改变样品的溶解度，或获得或改善结晶样品。

Features 特征
Developed at Hampton Research  汉普顿公司研究开发
For use with either membrane or soluble proteins  用于膜或可溶性蛋白质
Compatible with vapor diffusion, microbatch, and free interface diffusion methods         兼容蒸汽扩散，微批和自由界面扩散方法。
96 Deep Well block format     96深井区块格式
Classification of Detergents in screen  筛分中洗涤剂的分类
Ionic  离子
Non-ionic  非离子
Zwitterionic  两性离子
Non-detergent Sulfobetaines  非洗涤剂磺酸盐

Description
Note: HR2-407 Detergent Screen is a new formulation and has replaced the original HR2-406 Detergent Screen HT.

For crystallization to take place, a protein must be soluble in aqueous solution. Ideally the sample should be homogeneous, monodisperse, and in a state of aggregation conducive to interactions which will promote the nucleation and subsequent growth of a crystal. Unfavorable aggregation can compete with, obstruct, and prevent the normal ordering or the sample into a crystal. Many proteins, typically membrane proteins, membrane associated proteins, and soluble proteins contain hydrophobic residues on the surface which can lead to non-specific aggregation, a deterrent to solubility and crystallization. Mild, biological detergents can perturb and manipulate hydrophobic sample-sample and sample-solvent interactions. Incorporating detergents into the solubilization or crystallization reagent during screening and optimization is a popular and effective strategy for identifying conditions which promote and enhance solubility and crystallization. Besides improving the crystallization properties for some proteins and nucleic acids, detergents have also been shown to alleviate problems of twinning and secondary nucleation and can also produce different crystal forms.

Since it can be difficult to impossible a priori to predict and select which detergent or solubilizing agent will minimize the general nonspecific interactions yet at the same time sustain the specific interactions necessary for crystallization, the screening of a portfolio of detergent and solubilizing reagents is an efficient and effective strategy to identify the appropriate and best detergent reagent. The Detergent Screen is a set of solubilization and crystallization specific, mild, biological detergent reagents in a ready to use format at concentrations appropriate for solubility and crystallization screens. The Detergent Screen format allows one to identify a detergent effect as well as help select the best detergent reagent.

Each Detergent Screen kit contains 96 unique detergents. 0.25 ml of detergent solution is formulated at 10 times the reported CMC (unless otherwise noted) in sterile filtered deionized water. The Detergent Screen kit contains 96 solutions in a single Deep Well block, a single sheet of AlumaSeal II Sealing Film, User Guide, Formulations, and Scoring Sheet.

CAT NO NAME DESCRIPTION
HR2-407 Detergent Screen 0.25 ml, Deep Well block format

Detergent Screen HT Individual Reagents  0.5 ml

Crystal of alpha amylase

Membrane protein crystallized with beta D-glucopyranoside. Karl Brillet, IREBS, Illkirch, France.

Membrane protein crystallized with CHESO (2- hydroxyethyloctylsulfoxide. Karl Brillet, IREBS, Illkirch, France.

Applications

Individual Detergent Screen reagents for screening, optimization

Features
Individual reagents from the original HR2-406 Detergent Screen HT
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

0.5 ml volume
Description
Individual reagents from the original HR2-406 Detergent Screen HT supplied in a volume of 0.5 milliliter in a sterile polypropylene tune with o-ring seal. All detergents formulated in deionized, sterile filtered Type 1 Laboratory Water. Container is purged with argon prior to closure.

CAT NO NAME DESCRIPTION
HR2-406-01 Detergent Screen 01 (A1) 0.5ml 10% w/v BAM
HR2-406-02A Detergent Screen 02 (A2) 0.5ml 50.0 mM n-Dodecyl-ß-iminodipropionic acid, monosodium salt
HR2-406-02B Detergent Screen 02 (A2) 0.5ml 0.10 mM Lauryl maltose neopentyl glycol
HR2-406-03 Detergent Screen 03 (A3) 0.5ml 0.46 mM Dodecyltrimethylammonium chloride
HR2-406-04 Detergent Screen 04 (A4) 0.5ml 10.0 mM CTAB
HR2-406-05 Detergent Screen 05 (A5) 0.5ml 60.0 mM Deoxycholic acid, sodium salt
HR2-406-06 Detergent Screen 06 (A6) 0.5ml 80.0 mM Sodium dodecyl sulfate
HR2-406-07 Detergent Screen 07 (A7) 0.5ml 140.0 mM Sodium cholate
HR2-406-08 Detergent Screen 08 (A8) 0.5ml 144.0 mM Sodium dodecanoyl sarcosine
HR2-406-09 Detergent Screen 09 (A9) 0.5ml 10% w/v ANAPOE®-X-305
HR2-406-10 Detergent Screen 10 (A10) 0.5ml 10% w/v IPTG
HR2-406-11 Detergent Screen 11 (A11) 0.5ml 0.006 mM n-Hexadecyl-ß-D-maltoside
HR2-406-12 Detergent Screen 12 (A12) 0.5ml 10% w/v ANAPOE®-58
HR2-406-13 Detergent Screen 13 (B1) 0.5ml 0.10 mM n-Tetradecyl-ß-D-maltoside
HR2-406-14 Detergent Screen 14 (B2) 0.5ml 10% w/v ANAPOE®-80
HR2-406-15 Detergent Screen 15 (B3) 0.5ml 0.33 mM n-Tridecyl-ß-D-maltoside
HR2-406-16 Detergent Screen 16 (B4) 0.5ml 0.50 mM ANAPOE®-C12E9
HR2-406-17 Detergent Screen 17 (B5) 0.5ml 10% w/v ANAPOE®-20
HR2-406-18 Detergent Screen 18 (B6) 0.5ml 0.90 mM Thesit®
HR2-406-19 Detergent Screen 19 (B7) 0.5ml 10% w/v ANAPOE®-35
HR2-406-20 Detergent Screen 20 (B8) 0.5ml 10% w/v ANAPOE®-C13E8
HR2-406-21 Detergent Screen 21 (B9) 0.5ml 1.1 mM ANAPOE®-C12E8
HR2-406-22 Detergent Screen 22 (B10) 0.5ml 1.7 mM n-Dodecyl-ß-D-maltoside
HR2-406-23 Detergent Screen 23 (B11) 0.5ml 1.9 mM CYMAL®-7
HR2-406-24 Detergent Screen 24 (B12) 0.5ml 10% w/v ANAPOE®-X-114
HR2-406-25 Detergent Screen 25 (C1) 0.5ml 10% w/v ANAPOE®-C12E10
HR2-406-26 Detergent Screen 26 (C2) 0.5ml 3.0 mM Sucrose monolaurate
HR2-406-27 Detergent Screen 27 (C3) 0.5ml 5.6 mM CYMAL®-6
HR2-406-28 Detergent Screen 28 (C4) 0.5ml 5.9 mM n-Undecyl-ß-D-maltoside
HR2-406-29 Detergent Screen 29 (C5) 0.5ml 10% w/v ANAPOE®-X-405
HR2-406-30 Detergent Screen 30 (C6) 0.5ml 9.0 mM TRITON® X-100
HR2-406-31 Detergent Screen 31 (C7) 0.5ml 10% w/v ANAPOE®-C10E6
HR2-406-32 Detergent Screen 32 (C8) 0.5ml 9.0 mM n-Decyl-ß-D-thiomaltoside
HR2-406-33 Detergent Screen 33 (C9) 0.5ml 10.2 mM Octyl maltoside, fluorinated
HR2-406-34 Detergent Screen 34 (C10) 0.5ml 10% w/v ANAPOE®-C10E9
HR2-406-35 Detergent Screen 35 (C11) 0.5ml 14.0 mM Big CHAP, deoxy
HR2-406-36 Detergent Screen 36 (C12) 0.5ml 18.0 mM n-Decyl-ß-D-maltoside
HR2-406-37 Detergent Screen 37 (D1) 0.5ml 20.0 mM LDAO
HR2-406-38 Detergent Screen 38 (D2) 0.5ml 25.0 mM n-Decanoylsucrose
HR2-406-39 Detergent Screen 39 (D3) 0.5ml 1.5 mM n-Nonyl-ß-D-thioglucoside
HR2-406-40 Detergent Screen 40 (D4) 0.5ml 32.0 mM n-Nonyl-ß-D-thiomaltoside
HR2-406-41 Detergent Screen 41 (D5) 0.5ml 50.0 mM CYMAL®-5
HR2-406-42 Detergent Screen 42 (D6) 0.5ml 60.0 mM n-Nonyl-ß-D-maltoside
HR2-406-43 Detergent Screen 43 (D7) 0.5ml 65.0 mM n-Nonyl-ß-D-glucoside
HR2-406-44 Detergent Screen 44 (D8) 0.5ml 70.0 mM HEGA®-10
HR2-406-45 Detergent Screen 45 (D9) 0.5ml 70.0 mM MEGA-10
HR2-406-46 Detergent Screen 46 (D10) 0.5ml 71.0 mM C8E5
HR2-406-47 Detergent Screen 47 (D11) 0.5ml 76.0 mM CYMAL®-4
HR2-406-48 Detergent Screen 48 (D12) 0.5ml 80.0 mM C8E4
HR2-406-49 Detergent Screen 49 (E1) 0.5ml 85.0 mM n-Octyl-ß-D-thiomaltoside
HR2-406-50 Detergent Screen 50 (E2) 0.5ml 90.0 mM n-Octyl-ß-D-thioglucoside
HR2-406-51 Detergent Screen 51 (E3) 0.5ml 100.0 mM Hexaethylene glycol monooctyl ether
HR2-406-52 Detergent Screen 52 (E4) 0.5ml 104.0 mM DDAO
HR2-406-53 Detergent Screen 53 (E5) 0.5ml 115.0 mM C-HEGA®-11
HR2-406-54 Detergent Screen 54 (E6) 0.5ml 10% w/v Pluronic® F-68
HR2-406-55 Detergent Screen 55 (E7) 0.5ml 195.0 mM HECAMEG®
HR2-406-56 Detergent Screen 56 (E8) 0.5ml 200.0 mM n-Octyl-ß-D-glucoside
HR2-406-57A Detergent Screen 57 (E9) 0.5ml 244.0 mM n-Octanoylsucrose
HR2-406-57B Detergent Screen 57 (E9) 0.5ml 250.0 mM Sulfobetaine 3-10
HR2-406-58 Detergent Screen 58 (E10) 0.5ml 250.0 mM MEGA-9
HR2-406-59 Detergent Screen 59 (E11) 0.5ml 275.0 mM 2,6-Dimethyl-4-heptyl-ß-D-maltopyranoside
HR2-406-60 Detergent Screen 60 (E12) 0.5ml 290.0 mM n-Heptyl-ß-D-thioglucopyranoside
HR2-406-61 Detergent Screen 61 (F1) 0.5ml 0.1% w/v n-Octyl-ß-D-galactopyranoside
HR2-406-62 Detergent Screen 62 (F2) 0.5ml 345.0 mM CYMAL®-3
HR2-406-63 Detergent Screen 63 (F3) 0.5ml 350.0 mM C-HEGA®-10
HR2-406-64 Detergent Screen 64 (F4) 0.5ml 390.0 mM HEGA®-9
HR2-406-65 Detergent Screen 65 (F5) 0.5ml 400.0 mM Dimethyloctylphosphine oxide
HR2-406-66 Detergent Screen 66 (F6) 0.5ml 790.0 mM MEGA-8
HR2-406-67 Detergent Screen 67 (F7) 0.5ml 1.08 M C-HEGA®-9
HR2-406-68 Detergent Screen 68 (F8) 0.5ml 1.09 M HEGA®-8
HR2-406-69 Detergent Screen 69 (F9) 0.5ml 1.20 M CYMAL®-2
HR2-406-70 Detergent Screen 70 (F10) 0.5ml 2.50 M n-Hexyl-ß-D-glucopyranoside
HR2-406-71A Detergent Screen 71 (F11) 0.5ml 6.6% w/v C-HEGA®-8
HR2-406-71B Detergent Screen 71 (F11) 0.5ml 45 mM Tripao
HR2-406-72 Detergent Screen 72 (F12) 0.5ml 10% w/v CYMAL®-1
HR2-406-73 Detergent Screen 73 (G1) 0.5ml 0.5 M NDSB-195
HR2-406-74 Detergent Screen 74 (G2) 0.5ml 0.5 M NDSB-201
HR2-406-75 Detergent Screen 75 (G3) 0.5ml 0.5 M NDSB-211
HR2-406-76 Detergent Screen 76 (G4) 0.5ml 0.5 M NDSB-221
HR2-406-77 Detergent Screen 77 (G5) 0.5ml 0.5 M NDSB-256
HR2-406-78 Detergent Screen 78 (G6) 0.5ml 4.0 mM ZWITTERGENT® 3-14
HR2-406-79 Detergent Screen 79 (G7) 0.5ml 15.0 mM n-Dodecyl-N,N-dimethylglycine
HR2-406-80 Detergent Screen 80 (G8) 0.5ml 15.0 mM FOS-Choline®-12
HR2-406-81 Detergent Screen 81 (G9) 0.5ml 22.0 mM FOS-Choline®-8, fluorinated
HR2-406-82 Detergent Screen 82 (G10) 0.5ml 32.10 mM n-Undecyl-N,N-Dimethylamine-oxide
HR2-406-83 Detergent Screen 83 (G11) 0.5ml 40.0 mM ZWITTERGENT® 3-12
HR2-406-84 Detergent Screen 84 (G12) 0.5ml 43.0 mM DDMAB
HR2-406-85 Detergent Screen 85 (H1) 0.5ml 52.5 mM FOS-MEA®-10
HR2-406-86 Detergent Screen 86 (H2) 0.5ml 80.0 mM CHAPS
HR2-406-87 Detergent Screen 87 (H3) 0.5ml 80.0 mM CHAPSO
HR2-406-88 Detergent Screen 88 (H4) 0.5ml 110.0 mM FOS-Choline®-10
HR2-406-89 Detergent Screen 89 (H5) 0.5ml 190.0 mM n-Decyl-N,N-dimethylglycine
HR2-406-90 Detergent Screen 90 (H6) 0.5ml 395.0 mM FOS-Choline®-9
HR2-406-91 Detergent Screen 91 (H7) 0.5ml 400.0 mM ZWITTERGENT® 3-10
HR2-406-92 Detergent Screen 92 (H8) 0.5ml 430.0 mM CYCLOFOS™-3
HR2-406-93 Detergent Screen 93 (H9) 0.5ml 1.14 M FOS-Choline®-8
HR2-406-94 Detergent Screen 94 (H10) 0.5ml 10% w/v ZWITTERGENT® 3-08
HR2-406-95 Detergent Screen 95 (H11) 0.5ml 7.0 mM LysoFos™ Choline 12
HR2-406-96A Detergent Screen 96 (H12) 0.5ml 70.0 mM LysoFos™ Choline 10
HR2-406-96B HR2-406-96 – Detergent Screen 96 (H12) 0.5ml 0.36 mM LysoFos™ Choline 14

Detergent Screen Individual Reagents
0.5 ml
MembFac • Crystal Screen Lite • MembFac HT
Primary biased sparse matrix crystallization screen for membrane proteins and proteins with limited solubility

Hampton 石蜡和硅润滑油Paraffin & Silicon Oil

发表于由

Hampton 石蜡和硅润滑油Paraffin & Silicon Oil

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Optimize Reagents > Optimize – Oils for Crystallization > Paraffin & Silicon Oil

Applications  应用
Microbatch crystallization    microbatch 结晶
Features  特征
Under oil crystallization 在油中结晶
Protect the sample from oxidation  保护样品免于氧化
Screen different temperatures without condensation   屏幕不同温度下不凝结
Description   描述 
Oils used for microbatch and modified microbatch crystallization under oil.

使用的油和改性 microbatch microbatch 结晶油。

Al’s Oil is a 50:50 (volume:volume) mixture of Paraffin Oil and Silicon Oil. Al’s Oil is named after it’s inventor, Allan D’Arcy.

Al 是机油的 50 ∶ 50 (体积 ∶ 体积) 混合物的石蜡油和硅油。Al 的石油是其发明者的名字命名 ， Allan D ‘Arcy) 。

Paraffin oil
Synonym: Mineral oil
CAS Number [8042-47-5]
EC Number 232-455-8
RTECS PY8047000
MDL Number MFCD00131611
Appearance Colorless, clear, viscous liquid
Refractive Index n20/D 1.467 (literature) n20/D 1.468
IR Spectroscopy Grade
Density 0.838 g/mL at 25°C (literature) 0.85 g/ml at 20°C
Viscosity 40-42 CST at 25°C

Silicon Oil 
Synonyms: Octamethyltrisiloxane
CAS Number: 107-51-7
Appearance: Colorless, clear viscous liquid
Melting Point: -86°C
Boiling Point: 152°C
Refractive Index: 1.38 at 25°C
Viscosity: 1 CST at 25°C
Specific Gravity: 0.82 at 25°C
Density: 0.82 g/mL at 25°C
Surface Tension: 17.4 mN/m

Hampton 石蜡和硅润滑油Paraffin & Silicon Oil

CAT NO NAME DESCRIPTION
HR3-411 Paraffin Oil 100% – 250 ml
HR3-421 Paraffin Oil 100% – 1 L
HR3-415 Silicon Oil 100% – 250 ml
HR3-423 Silicon Oil 100% – 1 L
HR3-413 Al’s Oil (50:50 Paraffin:Silicon) – 250 ml
HR3-417 Combo Oil Pack (Paraffin, Al’s, & Silicon Oil) – 250 ml of each

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正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。

Hampton 产生蛋白质晶体的种子试剂盒 Seed Bead™ Kits

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Hampton 产生蛋白质晶体的种子试剂盒 Seed Bead™ Kits

Hampton research蛋白结晶试剂代理–上海金畔生物
代理优势：货期3-4周。
欢迎访问Hampton research官网或者咨询我们获取更多产品信息。

Tools & Seeding > Seed Bead > Seed Bead™ Kits

应用

  • 产生蛋白质晶体的种子

特征

  • 容易产生一致的种子
  • 用连续稀释法控制种子进入滴滴的数量。
  • 微种基质筛选(MMS)种子库
  • 聚四氟乙烯、不锈钢、硅酸锆陶瓷或玻璃种子珠

描述

种子珠套件用于创建种子库，以执行后续的种子试验。种子珠袋包含24个种子珠管，这是一种特殊的1.5毫升微离心管，形状可以在混合过程中将种子保持在样品中。每个管配置聚四氟乙烯，不锈钢，锆硅酸盐陶瓷，或玻璃种子珠。

种子允许在亚稳态区域生长晶体。在这个区域的结晶提供了控制，重现性和提高成功结晶实验的可能性。另外，晶体可以从种子中生长，但不能自发成核。通过将种子或溶液放置在一滴饱和到亚稳态区域的种子中，人们可以利用种子生长更大的单晶。通过控制引入液滴的种子数量，就可以控制生长的晶体数量。实际上不可能测量和知道引入一滴种子的数量，但通过对浓缩种子进行连续稀释，就可以控制滴滴中生长的晶体数量。

利用种子珠套件，我们可以为后续的播种实验创建水晶种子库。晶体被放置在带有种子珠的微离心管中，可以是涡状的，也可以是声纳的，以产生种子。然后，通过进行系列稀释，可以控制实验中晶体的数量和尺寸。

种子珠工具包有助于为自动化和半自动微型播种准备种子储存(D‘Arcy，2007年；Harlos，2008年)。

各种子珠袋(HR2-320)包含24个特殊的微离心管，每管包含一个单一的3mm聚四氟乙烯种子珠。

各种子珠钢盒(HR4-780)包含24根特殊的微离心管，每管含有6颗1.6毫米不锈钢种子珠。

各种子珠陶瓷盒(HR4-781)包含24个特殊的微离心管，每管含有10颗1.0毫米硅酸锆陶瓷种子珠。

各种子珠玻璃盒(HR4-782)包含24个特殊的微离心管，每管含有10颗1.0毫米的玻璃种子珠。

APPLICATIONS

  • Generate seeds of protein crystals

FEATURES

  • Easily generate consistent seed stocks
  • Use serial dilution to control the number of seeds introduced into the drop
  • Microseed matrix screening (MMS) seed stock
  • PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Bead

DESCRIPTION

The Seed Bead kits are used to create a seed stock for performing subsequent seeding experiments. The Seed Bead kits contains 24 Seed Bead Tubes, a special 1.5 ml microcentrifuge tube, shaped to maintain the seed in the sample during mixing. Each tube is configured with a PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Beads.

Seeding allows one to grow crystals in the metastable zone. Crystallization in this zone provides control, reproducibility and an improved likelihood of a successful crystallization experiment. Also, crystals can grow from seeds but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated to the metastable zone, one can use the seeds to grow larger single crystals. By controlling the number of seeds introduced into the drop, one can control the number of crystals grown. It is not practically possible to measure and know the number of seeds introduced to a drop, but by performing serial dilutions from a concentrated seed stock, one can control the number of crystals grown in the drop.

Using the Seed Bead kits, one can create crystal seed stock for subsequent seeding experiments. Crystals are placed in the microcentrifuge tube with the Seed Bead(s) and either vortexed or sonicated to generate a seed stock. Then by performing serial dilutions, one can control the number and size of crystals in the experiment.

The Seed Bead kit is useful for the preparation of seed stocks for automated and semi-automated microseeding (D’Arcy 2007, Harlos 2008).

Each Seed Bead Kit (HR2-320) contains 24 special microcentrifuge tubes, each tube containing a single 3mm PTFE Seed Bead.

Each Seed Bead Steel Kit (HR4-780) contains 24 special microcentrifuge tubes, each tube containing a six 1.6 mm Stainless Steel Seed Beads.

Each Seed Bead Ceramic Kit (HR4-781) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Zirconium Silicate Ceramic Seed Beads.

Each Seed Bead Glass Kit (HR4-782)

PTFE - Steel - Ceramic - Glass

HR2-320 Seed Bead Kit 24 tubes with PTFE Seed Bead
HR4-780 Seed Bead Steel Kit 24 tubes with Steel Seed Beads
HR4-781 Seed Bead Ceramic Kit 24 tubes with Ceramic Seed Beads
HR4-782 Seed Bead Glass Kit 24 tubes with Glass Seed Beads

Hampton Dialysis Buttons 透析按钮

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Hampton Dialysis Buttons 透析按钮

透析按钮

应用

  • 透析结晶，蛋白质折叠，小容量样品透析

特征

  • 低容量透析
  • 适用于24个井板
  • 一遍又一遍地使用

描述

在透析法中，样品被半透膜从“沉淀剂”中分离出来，这种膜允许小的试剂分子通过，但防止生物大分子穿过膜。1-3

透析按钮是注射成型的聚苯乙烯，大小与一个小按钮。样品被放置在这个房间，以便在按钮顶部形成一个微小的液体穹顶。透析膜被放置在按钮/样本的顶部，并以O形环保持在适当的位置。O形环被透析按钮中的凹槽固定在适当的位置.透析按钮配有O形环.5公分至100升大小的透析纽扣的总高度约为6毫米，外径约为10毫米而没有O形环，以及直径约为12毫米的O形环。200毫米和350毫升级透析按钮的总高度约为8毫米，外径约为15毫米，没有O形环，而O形环的直径约为17毫米。每个透析按钮包括一个塑料高尔夫球三通形喷头，以协助将透析膜应用到按钮上，并与所提供的o-环固定在一起。

Dialysis Buttons

APPLICATIONS

  • Crystallization by dialysis, protein folding, & small volume sample dialysis

FEATURES

  • Low volume dialysis
  • Fits in 24 well plates
  • Use over and over again

DESCRIPTION

In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3

Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

CAT NO NAME DESCRIPTION
HR3-336 Dialysis Buttons Sampler 5 of each size listed below
HR3-314 5 µl Dialysis Button 50 pack
HR3-316 10 µl Dialysis Button 50 pack
HR3-318 15 µl Dialysis Button 50 pack
HR3-320 20 µl Dialysis Button 50 pack
HR3-326 50 µl Dialysis Button 50 pack
HR3-328 100 µl Dialysis Button 50 pack
HR3-330 200 µl Dialysis Button 50 pack
HR3-332 350 µl Dialysis Button 50 pack

RELATED ITEM(S)

REFERENCES

1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).

2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).

3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.

4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

Hampton 结晶工具 CryoCane Color Coders

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Hampton 结晶工具 CryoCane Color Coders

Applications
Identify & organize CryoCanes
Features
Made of aluminum
Compatible with the CrystalCap systems
Description 
Colored aluminum tabs slip into the ends of CryoCane holders to identify a set of vials, a researcher’s work, or sample. Flat writing surface allows for additional identification. Available in white, dark blue, green, red or a sampler. The Sample HR4-722 contains 12 each of white, yellow, light blue, green and red for a total of 60 CryoCane Color Coders.

Hampton 结晶工具 CryoCane Color Coders

CAT NO NAME DESCRIPTION
HR4-713 CryoCane Color Coder – White 12 pack
HR4-717 CryoCane Color Coder – Dark Blue 12 pack
HR4-719 CryoCane Color Coder – Green 12 pack
HR4-721 CryoCane Color Coder – Red 12 pack
HR4-722 CryoCane Color Coder – Sampler 60 pack (12 of each color)

Hampton 蛋白结晶优化试剂 Optimization Screens

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Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性

Protein specific agents for optimization of solubility & stability  

2、Solubility & Stability Screen 2   离子缓冲液

Buffer, pH & ionic strength screen for optimization of solubility & stability

3、Slice pH    PH缓冲液

pH & buffer screen for optimization of crystals or sample solubility & stability

4、Additive Screen  96孔板添加剂试剂盒

96 compound screen for optimization of crystal size & quality

5、Silver Bullets • Silver Bullets Bio

Small molecule additive screen for optimization of crystal size & quality

6、Detergent Screen

Detergent screen for optimization of crystals or sample solubility & stability

7、Ionic Liquid Screen

Ionic liquid additive screen for optimization of crystal size & quality

8、StockOptions Buffer Kits

Titrated buffer sets for optimization of crystals or sample solubility & stability

9、StockOptions Polymer

n of crystal size & quality

10、StockOptions Salt

Salt reagent kit for optimization of crystal size & qualit

11、Polymer reagent kit for optimizatioy

Proti-Ace

In situ proteolysis kit protein for crystallization screening & optimization

12、Reductive Alkylation

Reductive alkylation kit for crystallization screening & optimization

13、Silica Hydrogel Kit

pH neutral silica hydrogel matrix crystallization

14、Low Melting Agarose

Agarose gel matrix crystallization

15、CryoPro

Water soluble cryoprotectant reagent set

16、Heavy Atom Screens

Heavy atom kits for multiple isomorphous replacement

17、I3C Phasing Kit

Produce heavy atom derivative of biological macromolecules for phasing